A post-transcriptional mechanism of gene-regulation/silencing mediated by small RNAs (siRNA)
5
votes
1answer
107 views
How to Design an siRNA Experiment?
I'm going to undertake an siRNA experiment soon, but I have only read about them. I want to address the role an enzyme plays in processing a protein.
From what I understand, I will need to pick two ...
0
votes
0answers
68 views
What is RNAi and how is it useful? [closed]
My biotechnology book doesn't explain RNAi and its usefulness very well. I know that it is used to silence certain genes, but how does it do that and what can we do with that?
14
votes
1answer
318 views
Can siRNA induce DNA methylation in mammalian cells?
Some years ago Hiroaki Kawasaki and Kazunari Taira published an article called "Induction of DNA methylation and gene silencing by short interfering RNAs in human cells" in Nature:
In plants, ...
9
votes
2answers
156 views
bi-directional transcription experiment
We suspect a bi-directional transcription event is happening at a locus in our organism where two genes are directly adjacent to each other. The annotation data is not well established. The intergenic ...
