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3
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2answers
32 views

Cell line immortalisation biosafety

Cell lines are often immortalised by artificial expression of proteins which specifically cause the knockout of important cancer suppression genes or the activation of proto-oncogenes. Cellular ...
4
votes
0answers
81 views

Do DNA supply houses maintain a watch list for dangerous oligos (Anthrax, Smallpox, etc.)?

The recent work by DeLoache, et al. on a synthetic opiate-precursor production pathway in yeast has generated a lot of scare stories in the media about people homebrewing heroin as easily as they ...
4
votes
1answer
28 views

Wearing sunglasses during a solar eclipse [closed]

Note: I had asked this on Physics, but it is off-topic there due to being about safety. On the BBC's guide to eclipse-watching, Dr Lucie Green says: Watching an eclipse with normal sunglasses ...
1
vote
2answers
59 views

How to safely sterilize urea-containing growth medium?

I'm using urea-containing growth medium for experiments with bacteria (1-2 l/day). After the experiment, the growth medium has to be sterilized and disposed. I did this so far by autoclaving, but our ...
3
votes
1answer
64 views

Safety of using industrial water for hand washing

Industrial water taps are present in most biology laboratories I have been too. There are accompanied by a sign stating "do not drink". So obviously it is not a good idea to drink it. However I ...
6
votes
2answers
767 views

Just how dangerous is ethidium bromide?

A common precaution for preparing EtBr-agarose gels is to add EtBr only after the gel solution has cooled somewhat, because EtBr is a dangerous mutagen. Supposedly, adding it to a hot liquid allows ...
8
votes
1answer
279 views

How do I put on a labcoat?

Let's assume I am wearing a labcoat for two reasons: Prevent the various bacteria, proteins, skin cells and substances on my clothes and my skin from contaminating my experiments. Prevent various ...
6
votes
4answers
292 views

What kind of microscope for ML/biological research?

I am a computer science student, focusing on machine learning applications. I have been always interested in biology but I lack any training in it. Now, I had an idea that I could introduce myself to ...
7
votes
2answers
494 views

Can a carnivorous animal consume plants with no adverse effects?

While I know that in nature, carnivorous animals are poorly suited to eat plants (largely due to having sharp teeth, not grinding teeth, as far as I know), I was wondering if, in an emergency ...
5
votes
1answer
298 views

Why do carrots spoil so fast?

The carrots we buy from a supermarkets spoil very fast, albeit giving many consumers a false sense of preservability due to their structural rigidity. The answer extends to many other vegetables many ...
4
votes
2answers
1k views

Neutralizing TCA washes [closed]

Trichloroacetic Acid (TCA) is commonly used for protein precipitation but the wash waste needs to be neutralized prior to disposal. What exactly is required to effectively neutralize TCA waste? Do I ...