Tagged Questions
-1
votes
0answers
33 views
Only the lower half of an SDS-PAGE shows visible bands
I have a polyacrylamide gel, 12%. After the run, the bands are seen only in the bottom half of the gel. Can anyone tell me why is that so ? The samples are dissolved in 8M urea.
5
votes
1answer
74 views
Does GTP-γS (GTP gamma S) bind all GTP-binding proteins?
I've just read an article Rab10 GTPase regulates ER dynamics and morphology - Nature Cell Biology 15, 169–178 (2013) doi:10.1038/ncb2647. In this paper, to identify Rab proteins in ER, first they ...
8
votes
1answer
1k views
When running gels what is the difference between constant volts or constant amps?
In general, you want to be consistent with running your gels either at constant volts or constant amps. However, it is very clear that during the progression of both PAGE and agarose gels, the free ...
7
votes
1answer
654 views
How do Proteins migrate in MES vs. MOPS
My gels look significantly different in MES (2-(N-morpholino)ethanesulfonic acid) and MOPS (3-(N-morpholino)propanesulfonic acid). That is to be expected. What I don't understand is why the simple ...
5
votes
1answer
616 views
How do SDS-PAGE gels differ in a Bis-Tris system vs. a Tris-Glycine system?
Protein migrate differently in Bis-Tris and Tris-Glycine gels. I was curious about the actual reasons why. Do certain gel systems result in a tighter resolution than others?
