Questions tagged [splicing]

Splicing is a modification of an RNA after transcription, in which intervening nucleotide sequence sequences (introns) are removed and the remaining sequences (exons) are joined.

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What is the ratio of pre-mRNA to mRNA?

What is the ratio of spliced to unspliced introns in a cell? I found, that the median half-life of a human mRNA is roughly 10h [1], while transcription and splicing each take around half a minute [2, ...
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Can elementary alternative splicing events be combined in more complex events?

I'm a computer scientist who is trying to understand alternative splicing. As the title says, I'm here to ask you if, from a biological point of view, elementary/basic alternative splicing events (...
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Can you splice out the first and last exon from pre-mRNA?

I think the answer is no because there are no introns before the first exon or after the last exon, although I am not sure if this assumption is correct. Thanks!
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Can nerves in the human body be spliced with a form of electric wire?

This may be a ridiculous question, and is hypothetical, but I'd like a well-supported answer. Is it possible to splice copper wire (for example) into the human nervous system? Would the charge ...
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non-coding RNA modelling in silico

I've been trying to model a non-coding RNA molecule which binds to a RNA binding protein. I found the DNA sequence for ncRNA from NCBI. I did not find the RNA seq of the same. Hence my doubt is, ...
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How often does pre-mRNA processing lead to midstream off-frame errors in eukaryotic mRNAs?

As I understand it, the splicing of introns from pre-mRNAs is not incredibly precise. I've only ever seen nucleotide position ranges given in any description of the involved processes. Since there is ...
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What percentage of introns are spliced in polyadenylated mRNAs?

I am asking a variation of a previously asked question: What percentage of RNA splicing happens during transcription? I am planning mRNA purification using poly(A) antisense-oligo pull-down and, for ...
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Why do organisms not just have more genes to account for the possible gene products generated from alternative splicing?

Alternative splicing can generate many gene products from single genes, by combinatorial inclusion / exclusion of introns / gene segments. What is the evolutionary advantage of having a complex ...
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