Set of methods to visualize certain properties (structure, gene expression, potential changes, etc.) of organisms or single cells.

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Does methanol fixation deform cultured cells?

I use methanol fixation (@ -20⁰C for 10 minutes) when performing immunofluorescence assays on cultured cells. Generally speaking, this results in very good antibody staining. However, the cells tend ...
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Hoechst red and blue spectra - what does the red emission tell us?

I understand that the blue spectrum is emitted when Hoechst 33342 binds to nuclear DNA. But what exactly is the red spectrum telling us? I read that the red spectrum can be indicative of apoptosis, ...
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80 views

How are new staining protocols designed for microscopy?

How are new staining protocols designed for microscopy? Is it manual work or does it rely on computer simulations? Say for example I thought the H&E staining does not bring enough contrast. How ...