Set of methods to visualize certain properties (structure, gene expression, potential changes, etc.) of organisms or single cells.

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187 views

Hoechst red and blue spectra - what does the red emission tell us?

I understand that the blue spectrum is emitted when Hoechst 33342 binds to nuclear DNA. But what exactly is the red spectrum telling us? I read that the red spectrum can be indicative of apoptosis, ...
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85 views

How are new staining protocols designed for microscopy?

How are new staining protocols designed for microscopy? Is it manual work or does it rely on computer simulations? Say for example I thought the H&E staining does not bring enough contrast. How ...
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12 views

Diffuse DAPI staining after time in tissue

My student is using ProLong Gold with DAPI (premixed) on sheep uterine tissue. We have used this reagent for some time with great results. Now, when she finishes her immunofluorescence protocol, ...
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29 views

Does bromophenol blue cause a shadow on agarose gels stained with SYBR Gold?

Bromophenol blue in loading dye is known to cast shadows/absorb emitted light signal from DNA on Ethidium bromide gels during imaging. But if SYBR Gold is instead used as the nucleic acid stain, does ...
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100 views

Barr body Giemsa staining

Why not all the female cheek cells show Barr Bodies when stained with Giemsa stain?? only 30 to 40 % female cells showed Barr Bodies . Why?
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24 views

Why is acid alcohol used as a decorizer in the acid fast stain procedure, as opposed to a neutral alcohol?

Is it to neutralize the negative components of the cell membrane, thus preventing the positively charged dye from adhering to the cell membrane?
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11 views

choice of negative stains in general

I have isolated a large molecular complex whose integrity is very sensitive to increasing ionic strength and thus had to be prepared for EM using low ionic strength solutions. My goal is image this by ...