Does anyone know if the T7 phage promoter is efficient in mammalian systems?
I am looking to find a highly competent E coli strain. I am making a library of a ~6.6kb plasmid and I am not getting high enough efficiency. Does anyone have a suggestion of a strain/protocol with ...
I was watching the video at this link: http://www.bbc.co.uk/news/science-environment-17436365 The speaker says that a cell is taken and its original DNA content is stripped out and replaced with ...
I would like to synthesize a 3.4kb gene (originally isolated from soil bacteria) and transform it into E. coli. There are several companies (DNA2.0, GeneScript, BlueHeron) which will synthesize the ...
I'm curious to know if this is physically feasible because during my reading up on synthetic biology and just general research i realise that life is capable of producing some exquisitely complex ...
This particular question has been of a great deal of interest to me, especially since it dives at the heart of abiogenesis.
Has anyone tried the chew back-anneal in vitro DNA assembly method (known as Gibson Assembly) for difficult sequences, like GC-rich sequences? How big constructs could you efficiently assemble? Did ...
What is the criticality of the ribosome binding site relative to the start codon in prokaryotic translation?
In prokaryotic translation, how critical for efficient translation is the location of the ribosome binding site, relative to the start codon? Ideally, it is supposed to be -7b away from the start. ...