This particular question has been of a great deal of interest to me, especially since it dives at the heart of abiogenesis.
What is the criticality of the ribosome binding site relative to the start codon in prokaryotic translation?
In prokaryotic translation, how critical for efficient translation is the location of the ribosome binding site, relative to the start codon? Ideally, it is supposed to be -7b away from the start. ...