Promoter from the bateriophage T7. The promoter is highly specific for the T7-RNA polymerase and widely used in the field of molecular biology as promoter for controlled gene expression.

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T7 phage promoter action in mammals

Does anyone know if the T7 phage promoter is efficient in mammalian systems?
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Why does the T7 RNA Polymerase require a reducing environment ie. DTT

Every bloody protocol suggests adding in DTT when doing in vitro RNA transcription. Why? The rationale seems to be that the cytoplasm traditionally has a reducing environment but as the only protein ...
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What determines a successful protein expression in E. coli?

Some proteins express well in a heterologous host; others- don't. A few requirements are known to determine the protein expression, like a strong promoter (like T7) for transcription and a strong ...