Promoter from the bateriophage T7. The promoter is highly specific for the T7-RNA polymerase and widely used in the field of molecular biology as promoter for controlled gene expression.

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10
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4answers
2k views

What determines a successful protein expression in E. coli?

Some proteins express well in a heterologous host; others- don't. A few requirements are known to determine the protein expression, like a strong promoter (like T7) for transcription and a strong ...
7
votes
1answer
2k views

Why does the T7 RNA Polymerase require a reducing environment ie. DTT

Every bloody protocol suggests adding in DTT when doing in vitro RNA transcription. Why? The rationale seems to be that the cytoplasm traditionally has a reducing environment but as the only protein ...
3
votes
1answer
421 views

T7 phage promoter action in mammals

Does anyone know if the T7 phage promoter is efficient in mammalian systems?
2
votes
3answers
69 views

T7 vs pBAD promoter strength

How do pBAD and T7 promoter strengths compare when pBAD is induced under conditions which would lead to its highest strength? Are there any papers that compare the two inducible promoter systems?
0
votes
1answer
57 views

Why does pETDuet-1 only have one T7 terminator but two T7 promoters?

Why does pETDuet-1 only have one T7 terminator but two T7 promoters (vector map below)? Is there any advantage of having only one terminator? There are going to be two mRNAs transcribed from this ...
0
votes
0answers
20 views

What is the highest number of genes expressed under one T7 promoter?

What is the highest number of genes expressed under one T7 promoter? Also, what is the longest polycistronic mRNA? I am interested in the context of heterologously expressing biosynthetic gene ...