The process that produces a complementary strand of RNA from a section of DNA or (rarely) other RNA.

learn more… | top users | synonyms

2
votes
3answers
579 views

Why isn't there a standard unit of promoter strength?

Sometimes in synthetic biology, we need to know rates of transcription of one promoter in relation to others (particularly inducible vs constitutive) in order to perform tasks like balancing ...
2
votes
2answers
600 views

Transcription factor binding site located in intron

I have noticed that some TF binding sites are located in the introns of the genes. I am puzzled about whether the TF only binds to DNA in the initiation stage of transcription and will detach during ...
2
votes
3answers
124 views

Why don't housekeeping genes have TATA Box regions in their promoter sequences?

Housekeeping genes are genes that are continuously transcribed. Like all other genes they have promoter sequences, but they don't have TATA box sequences that are used to specify from where ...
2
votes
1answer
34 views

Why is there no U3 snRNP in the spliceosome complex of transcription initiation?

snRNP U1,U2,U4,U5,U6 are present in the spliceosome but there is no mention of U3.
2
votes
1answer
38 views

Are both (partial) copies of DNA transcribed in S and G2 phases of cell cycle?

For a little less than half the cell cycle, a significant number of genes are represented twice (just before dividing). Does the cell differentiate between these DNA in any way or are transcription ...
2
votes
0answers
63 views

Multiple transcripts encoding for one protein

Trying to get a better understanding of the process of DNA to proteins. So when we have a gene, it is read from the 5' to 3' end, only translating the exons to mRNAs. A single gene can have multiple ...
2
votes
0answers
34 views

Link between macro lncRNA and DNA looping

I was wondering if anybody knows some publication about macro lncRNA (very long unspliced RNAs) or more generally a transcribed RNA that may lead to cis-DNA looping of genomic regions overlapped by ...
1
vote
2answers
1k views

Stop codons and exons?

If we had a hypotheical gene called gene exampleGene and this gene had 5 exons, labeled A, B, C, D, and E in that order on the chromosome, could it be the case that the stop codon for this gene be on ...
1
vote
1answer
241 views

What type of mutation causes Progeria?

I'm doing my High School biology final on Progeria, and am wondering what type of mutation causes this disease. I know that the LMNA gene codes for the "prelamin A" protein, and that protein contains ...
1
vote
1answer
125 views

How do DNA-binding proteins determine that they're binding to the correct DNA base pairs?

My professor posed this question to the class today - "How do DNA binding proteins specifically bind to base pairs?" He alluded to the different arrangements of hydrogen-bond donor and acceptors in ...
1
vote
2answers
43 views

identifying which SNPs sit in TFBS (Yeast)

i have a set of ~11k SNPs for Saccharomyces cerevisiae, baker's yeast and i would like to identify which ones of these sit in transcription factor binding sites and if they do information on the ...
1
vote
1answer
14 views

What was the first gene(s) found to code for ncRNA

I could not find a source that states what the first gene found to code for ncRNA was. Someone told me, however, it was a gene that coded for either rRNA or tRNA. To this point in time I have had no ...
1
vote
1answer
99 views

How is the rate of transcription influenced by temperature?

How is the rate of transcription influenced by temperature? More precisely, I am looking for an article who quantitatively measured the rate of transcription of an "average gene" and show how this ...
1
vote
1answer
70 views

Omics integration questions [closed]

Let's say that I have a population of Mus musculus in the lab. I divide it in a control group and a test group. The test group is constantly subjected to a stress (example: elevated UV radiation). ...
1
vote
1answer
12k views

Which enzyme catalyzes transcription and which translation?

My attempt: Transcription - RNA polymerase Translation - aminoacyl tRNA synthetase I know that the ribosome almost runs translation, but I don't think ribosomes are enzymes, which is why I went ...
1
vote
1answer
25 views

Transcription of Genes: Are Specific Transcription Factors + Enhancers Necessary?

I learned about transcription in my AP Biology class and we discussed how transcription occurs, but I was wondering whether transcription always requires the enhancers, activators (specific ...
1
vote
1answer
63 views

TSS of a gene vs TSS of a transcript

I have an annotation from gencode.vM1.annotation of a mouse. Exons, genes, transcripts and UTR are listed there. For each feature a start and end positions are present. I need to find a TSS for each ...
1
vote
1answer
94 views

What is the spacing between stop codon and transcription terminator?

When one tries to express a protein from a synthetic plasmid in E. coli, how many base pairs should there be between stop codon and the transcription terminator site?
1
vote
1answer
39 views

How does the MET gene work and what happens when the promoter region gets mutated?

I am doing research on inherited risk of Autism Spectrum Disorders(ASD) due to common Copy Number Variants(CNVs) One of the mutations is the 'CC' variant of Rs1858830 in the promoter region of the MET ...
1
vote
1answer
51 views

Are there U-rich transcriptional pause sites?

I've heard a statement in a talk that U-rich sequences cause transcriptional pausing. The intuitive explanation for this was that the UTP concentration is lower in the cell than the ATP concentration. ...
1
vote
1answer
82 views

How cotranscription events affect splicing?

I want to know how and how often does cootranscription takes place? Can transcription regulators influence splicing during cotranscription?
1
vote
1answer
155 views

Complementarity of cDNA

Strictly speaking, what is the definition of cDNA? This confuses me, since usually it is said to refer to DNA that is complementary to mRNA. Is this correct? Is it restricted to mature mRNA? I ...
1
vote
1answer
37 views

Where can I find a transcriptome of a normal uveal melanocyte?

I am looking for a database where I could find a transcriptome (say obtained with a microarray analysis) of one (or ideally many) normal uveal melanocytes ?
1
vote
0answers
21 views

Is DNA transcription inactive during mitosis ie no proteins are made? How does the cell survive?

I've read that during mitosis, DNA exists as heterochromatin, a form that is unable to be accessed for transcription. Does this mean no new proteins are made during what can be 20% of a cell's life? ...
1
vote
0answers
15 views

Are both chromosomes transcribed and translated? [duplicate]

Or is it somehow regulated if both or only one chromosome is transcribed/translated?
1
vote
0answers
19 views

Will bacterial promoter without either -10 or -35 sequence allow no transcription?

I have a strong constitutive promoter on a plasmid inside of E.Coli, which expresses a gene. Having either -10 or -35 sequence modified to random sequence, will it ensure zero transcription from that ...
1
vote
0answers
18 views

Are upstream activating factor (UAF) and upstream binding factor (UBF) the same thing?

During ribosome pre-40S and pre-60S synthesis, many sources state the importance of UAF or UBF in initiation complex of ribosome DNA transcription. None of the sources I've seen mentions the other ...
1
vote
0answers
27 views

How does Temperature influences the rate of protein turnover? [duplicate]

Question How (quantitatively speaking) does temperature influences rate of turnover of transcription factors? Which protein? As I am not looking for any accurate number I am talking about an ...
1
vote
0answers
43 views

What is the suitable terminology to describe this study approach?

I need to know the correct term(s) which are usually used in the parlance of both biology and bioinformatics for this study approach: About 11 transcripts were investigated using qPCR for a number of ...
0
votes
2answers
39 views

General transcription factors vs. DNA Binding transcription factors

I know that DNA binding transcription factors are trans-acting, but what about general transcription factors? Are they cis or trans acting?
0
votes
2answers
201 views

What is “transcribed strand”

This is an excerpt from a paper: T>C mutations at NpTpN trinucleotides, also found in hepatocellular carcinomas, shows strong transcriptional strand bias with more T>C mutations on the transcribed ...
0
votes
1answer
32 views

Time needed for transcription of DNA [closed]

How much time would it take to complete the transcription of an average-sized human gene?
0
votes
1answer
633 views

ATP required for cell processes [closed]

I haven't been able to find anything that tells me how much ATP is needed for DNA replication, transcription, and translation in humans, just papers that mention ATP used in those processes. I need ...
0
votes
1answer
29 views

Transcription when Chromosomes are Condensed

Are genes transcribed just as well when chromosomes are condensed? I want to design a screen that depends on genes not transcribed when chromosomes are condensed (to identify cells that can't ...
0
votes
1answer
48 views

How does RNA polymerase achieve higher processivity?

DNA polymerase uses a sliding clamp in order to replicate DNA. RNA polymerase does not require one. What allows RNA polymerase to hold on to DNA just like DNA polymerase?
0
votes
0answers
20 views

What is the difference between basal transcription and activated transcription?

My professor keeps mentioning these two forms of transcription and I'm completely confused as to what the difference is between them. I didn't even realise there were two different types of ...
0
votes
0answers
14 views

How many Stress Response Transcription Factors are there in yeast (and what are they)

I've Read that there are ~209 TFs in Yeast, but I'd like to know how many (and which) of these TFs are associated with Stress Responses? Is there a paper someone can link me to?
0
votes
0answers
15 views

translation rate regulated by transcription factors?

I'd like to know if there is in the litterature some examples of transcription factors capable to impact not just transcription but also translation rate so that both can be adjusted. I would be very ...