The process that produces a complementary strand of RNA from a section of DNA or (rarely) other RNA.

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62 views

Why and how is DNA synthesis so much faster then RNA synthesis?

DNA synthesis in E. coli is 20x faster than RNA synthesis at 1000nt/s vs 50nt/s. (Mirkin'05) I find that perplexing since DNA polymerization has better proofreading than the RNA variety, which ...
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18 views

Gene silencing: relative or absolute number of methylated CpG sites?

CpG content varies a lot across the human genome. What does determine a promoter to be silenced through DNA methylation: the fraction of CpG sites that are methylated or the total number of methylated ...
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64 views

Multiple transcripts encoding for one protein

Trying to get a better understanding of the process of DNA to proteins. So when we have a gene, it is read from the 5' to 3' end, only translating the exons to mRNAs. A single gene can have multiple ...
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34 views

Link between macro lncRNA and DNA looping

I was wondering if anybody knows some publication about macro lncRNA (very long unspliced RNAs) or more generally a transcribed RNA that may lead to cis-DNA looping of genomic regions overlapped by ...
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23 views

Is DNA transcription inactive during mitosis ie no proteins are made? How does the cell survive?

I've read that during mitosis, DNA exists as heterochromatin, a form that is unable to be accessed for transcription. Does this mean no new proteins are made during what can be 20% of a cell's life? ...
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19 views

Will bacterial promoter without either -10 or -35 sequence allow no transcription?

I have a strong constitutive promoter on a plasmid inside of E.Coli, which expresses a gene. Having either -10 or -35 sequence modified to random sequence, will it ensure zero transcription from that ...
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18 views

Are upstream activating factor (UAF) and upstream binding factor (UBF) the same thing?

During ribosome pre-40S and pre-60S synthesis, many sources state the importance of UAF or UBF in initiation complex of ribosome DNA transcription. None of the sources I've seen mentions the other ...
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43 views

What is the suitable terminology to describe this study approach?

I need to know the correct term(s) which are usually used in the parlance of both biology and bioinformatics for this study approach: About 11 transcripts were investigated using qPCR for a number of ...
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21 views

What is the difference between basal transcription and activated transcription?

My professor keeps mentioning these two forms of transcription and I'm completely confused as to what the difference is between them. I didn't even realise there were two different types of ...
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14 views

How many Stress Response Transcription Factors are there in yeast (and what are they)

I've Read that there are ~209 TFs in Yeast, but I'd like to know how many (and which) of these TFs are associated with Stress Responses? Is there a paper someone can link me to?
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15 views

translation rate regulated by transcription factors?

I'd like to know if there is in the litterature some examples of transcription factors capable to impact not just transcription but also translation rate so that both can be adjusted. I would be very ...