Yeasts are a polyphyletic group of unicellular fungi. Usually the term yeast is used synonymously with baker's yeast - Saccharomyces cerevisiae.

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Why would yeast grow but not produce alcohol?

I'm studying for my intro to microbiology test and I have a question asking why a culture of yeast responsible for producing alcohol would be growing, but not producing alcohol? The multiple choice ...
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Results of fermenting bakers yeast, Sugar and different categories of fruit peels

Based on this recipe I made three Jars of the fermented solution each containing: Papaya Peels Lemon+ Pomegranate peels Pineapple Peels In addition to above recipe I added 1 large tbsp of ...
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What is the best current understanding of how yeast transformation works?

I would like to get myself up to speed with what is currently known to science about yeast transformation. Specifically, transformation of plasmids and linear DNA fragments. I am particularly ...
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How does yeast dissolve in sugar?

There's this fascinating "kitchen experiment". Take a "brick" of baker's yeast (not dry yeast), and a couple teaspoons of sugar, put them together in a cup and stir together. There's no doubt both are ...
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Why does the stability of YAC increase with size?

From Lehninger Principles of Biochemistry : The stability of YAC increases with size (up to a limit). Those with inserts of less than 100000 are gradually lost during mitosis. Why does this ...
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Does yeast grow on LB agar plates?

I would like to grow E coli cells on cellophane on top of LB plate, and then grow yeast cells on the same LB plate. Does (or how well) yeast grow on LB plates? Is there a composition media that can be ...
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What are the effect of microtubule or microfilament inhibition on yeast expression profile?

I was wondering whether anyone has looked at what are the expression changes in yeast when the microtubule or the microfilament polymerization is inhibited? Have there been whole-genome studies?
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Book on yeast reproduction, evolution and on the use of yeast as model organism

I am looking for a good book in: Yeast reproduction mating types Recombination mating type determination mechanism Yeast evolution Yeast as model organism and its use in experimental biology It ...
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Why do yeast ferment Equal sweetener

I had my students conduct a fermentation experiment manipulating sugar amounts (number of sugar packets in solution) and measuring CO₂ production via capture in a balloon and measuring circumference ...
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Generalism - specialism index in microbial ecology

I am doing research in Lipid metabolism in different yeast species and I would like to look into ecology of them. I would like to classify them to a Generalist/specialist based on different ...
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Why did the yeast rise?

Here are the notes I made for an experiment I was doing (I didn't put it here as it is too long) (please ignore the numbers at the end of the pdf file) The graph looks like this: As you see on ...
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Sources for common laboratory Saccharomyces strains?

I'm used to working with bacteria - some of the more common laboratory strains sometimes come with an order from some vendors. What's the most convenient source to order common straings of ...
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Aerobe or facultative anaerobe organism that metabolizes acetate?

I'm assuming bacteria, but will take any suggestions. Organism must survive primarily on the acetate (plus trace elements), but I can give/take electrons, if necessary. Not interested in strict ...
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Isoprenoid Diphosphate Concentration in Yeast saccharomyces cerevisiae

Isoprenoid Diphosphate(IPP) is an important metabolites which is precursor in lot of secondary metabolites like Dolichol diphosphate, ubiquinone, prenylated proteins and carotenoid (not synthesized ...
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Carotenoid biosynthesis in yeast

Does Budding Yeast Sacchromyces cerevisiae produces significant amount of carotenoids? Have anybody estimated the ratio of flux going in branches 1. Cholesterol synthesis(via squalene) 2. Coenzyme Q6 ...
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How the concentration of an antibiotic is chosen for YPD medium?

I need to prepare YPD plates containing hygromycin, but I don't know which concentration I can use. I'll select transformants of Hansenula polymorpha.
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Proteins that are strongly overproduced in E. coli and S. cerevisiae?

I'm looking for some pointers to proteins that produce at really gigantic levels in E. coli and yeast (S. cerevisiae). Can anyone point to some champion proteins? Even in inclusion bodies and non ...
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Do Yeast Insertion constructs revert?

If I insert a new gene with a yeast integrating plasmid and select with a drop out culture once, can I assume that the newly integrated gene will stay in the strain without putting selective pressure ...
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Online toolkit that provides functional similarity scores (in the form of a matrix) between two functional gene sets in the context of gene ontology

Where can I find an online toolkit that provides functional similarity scores (in the form of a matrix) between two functional gene sets in the context of gene ontology? I have tried the following: ...
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Why is PEG important for efficient yeast transformation?

One way to do an yeast transformation is by using lithium acetate, a single-stranded carrier DNA, and PEG (1). I was wondering why is the polyethylene glycol important for the efficient ...
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What does the 34/70 in Saccharomyces pastorianus Weihenstephan 34/70 stand for?

I've searched everywhere. No Wikipedia page. No information on NCBI. I searched all occurrences of 34/70 in some primary research articles! The best I've found is this brewery forum where someone ...
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Auxotrophic complementation test with yeast

If auxotrophic strains grow on minimal media do they complement each other or not? Also, is complementation the same thing as complementing each other?
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What is the tRNA gene copy variation between different yeast strains?

I just started working on predicting steady-state protein levels from different codon usage bias measures. I have whole-genome sequences (it's NGS data) from different strains of S. cerevisiae and S. ...
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What is causing my problem with very low yields when isolating a 42kb yeast plasmid?

I have to isolate a large plasmid from yeast and transform it in E. coli. After transformation, I often get no colonies. One reason for that is the yeast mini prep hasn't worked or the DNA ...