Yeasts are a polyphyletic group of unicellular fungi. Usually the term yeast is used synonymously with the baker's yeast - Saccharomyces cerevisiae
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How the concentration of an antibiotic is chosen for YPD medium?
I need to prepare YPD plates containing hygromycin, but I don't know which concentration I can use. I'll select transformants of Hansenula polymorpha.
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Proteins that are strongly overproduced in E. coli and S. cerevisiae?
I'm looking for some pointers to proteins that produce at really gigantic levels in E. coli and yeast (S. cerevisiae). Can anyone point to some champion proteins?
Even in inclusion bodies and non ...
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Do Yeast Insertion constructs revert?
If I insert a new gene with a yeast integrating plasmid and select with a drop out culture once, can I assume that the newly integrated gene will stay in the strain without putting selective pressure ...
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Online toolkit that provides functional similarity scores (in the form of a matrix) between two functional gene sets in the context of gene ontology
Where can I find an online toolkit that provides functional similarity scores (in the form of a matrix) between two functional gene sets in the context of gene ontology?
I have tried the following:
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Why is PEG important for efficient yeast transformation?
One way to do an yeast transformation is by using lithium acetate, a single-stranded carrier DNA, and PEG (1). I was wondering why is the polyethylene glycol important for the efficient ...
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What does the 34/70 in Saccharomyces pastorianus Weihenstephan 34/70 stand for?
I've searched everywhere.
No Wikipedia page.
No information on NCBI.
I searched all occurrences of 34/70 in some primary research articles!
The best I've found is this brewery forum where someone ...
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200 views
Auxotrophic complementation test with yeast
If auxotrophic strains grow on minimal media do they complement each other or not? Also, is complementation the same thing as complementing each other?
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What is the tRNA gene copy variation between different yeast strains?
I just started working on predicting steady-state protein levels from different codon usage bias measures. I have whole-genome sequences (it's NGS data) from different strains of S. cerevisiae and S. ...
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What is causing my problem with very low yields when isolating a 42kb yeast plasmid?
I have to isolate a large plasmid from yeast and transform it in E. coli. After transformation, I often get no colonies. One reason for that is the yeast mini prep hasn't worked or the DNA ...
