0
votes
0answers
2 views

Forgot to vortex

Ugh. Did an immunofluorescence experiment last weekend, forgot to vortex both my primary and my secondary antibody solutions. And my final result looks dimmer than it should be. Is it possible that ...
1
vote
1answer
9 views

Why does the Pentose Phosphate Pathway predominate in erythrocytes?

Is it because glyceraldehyde -3-phosphate (a molecule which when enter glycolysis help produce ATP through substrate level phosphorylation) can be prepared without losing an ATP through this process?
0
votes
0answers
10 views

Influence of alcohol/hangovers on sex drive

I have noticed that whenever I go out and drink alcohol I'll have a much higher desire for sex with my hangover the next day. I'm harder and the sex is better (this is not a troll question btw, I'm ...
1
vote
1answer
95 views

How much do crocodiles eat?

How much do crocodiles eat? The closest I found to an answer was on German wikipedia, where it says that adult nile crocodiles eat "only about 50 full meals a year." While interesting, without knowing ...
1
vote
0answers
9 views

Absorption of nutrients in human beings

I know that Pulmonary Veins carry oxygenated blood from lungs to heart, and Pulmonary vein carry deoxygenated/CO2 rich blood from heart to lungs. Also, Arteries carry oxygen-rich blood from heart to ...
0
votes
1answer
4 views

Predator prey interaction

I went through a line in my text book which read 'But for predators, prey species could acheive very high population densities and cause ecosystem instability'... I was not able to understand the ...
0
votes
0answers
9 views

how to identify complexes

I have done a SILAC experiment and I have identified many proteins now I would like to know the possibilities to identify complexes (not using a databases). I know how to use databases to identify ...
0
votes
0answers
11 views

Why people have less hair than other primates?

Well as you can figure out, most primates such as monkeys, gorillas and chimpanzees, have all their body covered with fur. As we know they are evolutional "relatives" to us humans, but we dont have ...
1
vote
1answer
18 views
+50

Which ingested allergens can be transferred to a partner via bodily fluids?

Can some allergens, such as peanut traces, appear in bodily fluids (saliva, sweat, semen, vaginal secretion) after ingestion? I would like to know in particular intake of which allergens should be ...
0
votes
0answers
2 views

How to Study PR intervals as HRV Metrics?

I am trying G. Clifford book about ECG Data Analysis 2006. RR intervals are mostly used as HRV metrics. PR intervals not because of low magnitude of detection. I would like to get some samples of such ...
3
votes
1answer
533 views

Storing Taqman plates with polymerase in fridge before running

I'm going to be running some Taqman assays on cDNA generated from RNA collected from various samples, and will end up running a bunch of plates (right now the setup is 1/2 a plate per sample, ...
0
votes
1answer
42 views

Determination of genes to be modified in epigenetics [closed]

It seems that during reproduction particular genes are targeted for modification (mutation, deletion, insertion, etc.) given environmental inputs of either or both of the parents. If a creature was ...
4
votes
2answers
115 views

In DNA repair, how is it determined which strand contains the error?

DNA replication is more accurate than transcription (or RNA replication) because mechanisms exist for proof-reading and repair of DNA, but not for RNA. Consider a segment of a DNA strand, AGTC. Its ...
0
votes
0answers
29 views

How does DNA Polymerase make errors?

DNA Polymerase inserts the wrong nucleic acid base into DNA at a rate estimated to be between 1 in 107 and 1 in 108. How does it determine which base to match to the template strand, and how does it ...
5
votes
1answer
36 views

How much do urban pigeons move around cities?

Do they tend to live their lives in one area of the city or do they move around? For example, do pigeons born on London's Trafalgar Square live their whole lives centered around Trafalgar Square or ...
0
votes
1answer
39 views

Taking care of Birds: Unwanted species scaring away a less common one from a birdhouse

Near my house, I have set up a birdhouse, which has become home to a pair of Oriental Magpie Robins. They are not a common species in Delhi, India, and are generally shy of human interaction and avian ...
0
votes
1answer
22 views

ExAC Browser: What does a “dubious variant annotation” actually mean? [on hold]

I wonder how a "dubious" variant annotation is exactly defined in ExAC. I couldn't find any further information neither at the variant's page nor in the FAQs. [EDIT] Link to ExAC browser [EDIT] ...
1
vote
0answers
4 views

Could a large constrictor kill a small animal?

According to this article there was a python whose body circumference was 85cm. This means its diameter was 27cm. Would it be possible for this snake to constrict animals that are about the same ...
2
votes
1answer
58 views

What is membrane-partitioning free energy? Can it be simulated?

Firstly, is there a strict definition of the "membrane-partitioning free energy"? It is banded around in membrane biology, but I have never seen it strictly defined. The only non-scholarly site that ...
1
vote
0answers
7 views

O-Group names of Salmonella serotypes?

What liposaccharides are represented by the O-group designations of Salmonalla serovars in the Kauffman-White classification scheme? I mean to ask, how do the numbers in a Sal serovar (1, 9, & 12 ...
1
vote
0answers
367 views

Why do orcas eat only the tongue of whales, liver of sharks, etc.?

I noticed that although killer whales are clearly the top predator living in the present oceans, some of them appear to be very picky with their meals. After spending hours hunting down a whale, ...
1
vote
1answer
26 views

What is the reason for 'microcytic' anaemia?

I know the causes for microcytic anaemia are Fe deficiency, prolonged inflammation, Thalessemia, Sideroblastic etc. All these logically point to a decrease in Haemoglobin synthesis - either Haeme or ...
2
votes
0answers
14 views

Names for the degrees for symptoms from drought (or other damage) done to trees

Looking out my window, I see trees suffering from drought. I once knew the name for the degrees visible, but I have forgotten and can't find it on the net. One level was the tree being completely ...
1
vote
0answers
9 views

How can I perform a 1:1 sequence-structure alignment for a pdb template dimer and homologous dimer sequence?

I have: 1. A PDB dimer protein structure template of a real solved protein. 2. An unsolved, but highly homologous dimer protein (>90% sequence identity.) How can I use MODELLER to make a theoretical ...
-1
votes
0answers
10 views

Is there a way to convert/export track files (.trc file) generated from Media Cybernetics Image Pro Plus to .mat file type?

I am trying to export these files so that they can be used with a matlab based automated behavior classification software JAABA. At the very least I would like to figure out a way to read the format ...
0
votes
0answers
12 views

How is the acetone method different from QB buffer for extraction of plant protein?

I primarily work on animal tissue but I thought of running an SDS-PAGE for plant tissue just for fun. I searched for some protein extraction protocols online and I don't know which one to choose. One ...
1
vote
1answer
26 views

Amino Acid requirement + intake in relation to diet + meat type

I was arguing with a friend: I said: the Yulin festivals cannot be condemned by western culture, as we also kill animals in equally cruel ways. She said: not only is it cruel, but the dogs (and ...
0
votes
2answers
35 views

Spatial dimensions for an animal

I'm reading a review paper. They say: The position of an object in head-centered coordinates (that is, relative to an animal's head) can be defined along three axes: the medio-lateral (radial) ...
4
votes
2answers
75 views

Does a plant have to have leaves to produce fruit?

Is it possible that a plant can still produce fruit, even though it may not have leaves? Are there any plant species that can produce fruit without leaves?
-1
votes
0answers
12 views

How can I grow seedless strawberries [on hold]

Many people with diverticulitis are under the impression that they can't eat foods with seeds. Although this does not seem to research based the practice seems to persist. I want to know how I can ...
2
votes
0answers
43 views

Why did we evolve to control our breath?

I had this question in my mind and tried to think of why we would evolve to be able to control our breath. I remembered the Aquatic Ape Hypothesis, which makes a lot of sense and would explain it, ...
0
votes
0answers
28 views

Deletion of codons at the start of a sequence in preparation for heterogeneous expression. Why?

I am reading a patent where they isolate a gene from cDNA constructed from RNA extracted from plant matter. The subsequent step (in preparation for heterogeneous expression in E Coli.) puzzles me: ...
7
votes
1answer
70 views
+50

What makes worms' eggs float or sink?

In parasitology, while examining the stool sample, one important thing you do is test whether the eggs float on water or sink. We never did that since our coursework limits itself to microscopic ...
-3
votes
1answer
37 views

Can you naturally change your DNA that's passed to your offsprings? [on hold]

Is it possible to change the genes that you'll pass on to your offsprings by natural activity and behavior. For example if you do body building for quite a long time build strong muscles then would ...
3
votes
1answer
99 views

Glycerol 3-phosphate nomenclature

Why is Glycerol 3-phosphate named in such way? Shouldn't it be named as Glycerol 1-phosphate by proper IUPAC- nomenclature?
1
vote
1answer
68 views

True or False: Dideoxynucleotide sequence analysis Question

True or false: Dideoxynucleotide sequence analysis is a template-directed method that makes use of chain terminators that stop DNA synthesis because they lack a 2'OH group. The answer is false. ...
0
votes
1answer
272 views

0.1 M sodium citrate in 10% ethanol and DNA solubility

I understand how DNA precipitation works in the presence of salt (such as 0.3 M sodium acetate or 0.2 M sodium chloride) and alcohol (30 ~ 50 % isopropanol or 60 ~ 75% ethanol) However, in the Trizol ...
15
votes
2answers
140 views

What are the main mechanisms of interaction between the nervous and immune systems?

We know from pop science that our psychological states have an effect on our immune systems ("worrying ourselves sick", etc.), but what are the actual mechanisms through which our nervous systems pass ...
0
votes
0answers
4 views

Feedback mechanisms for the dynorphin-kappa opioid system?

(this is all happening in the brain). Also it's best to go context-first I think (1) Normally if you take an agonist drug that hits a receptor "x", eventually the brain will downregulate the density ...
0
votes
0answers
9 views

What is the difference between an uncompetitive, noncompetitive, and mixed inhibitor?

I can't seem to find a good resource online that clearly outlines the difference between an uncompetitive, noncompetitive, and mixed inhibitor (I understand competitive inhibitor though). More ...
5
votes
1answer
46 views

Is this a firefly?

It glows without interruption. This photo was taken near Barcelona, Spain. Thanks in advance.
3
votes
1answer
23 views

Japanese Crab Identification?

I saw a crab walking home from work the other night. We're by a river, but I was probably 500 feet away from the river when I saw this one. I apologize for the image quality, all I had was my terrible ...
7
votes
0answers
97 views

Identification of a mammal in cage

This unidentified animal was trapped in northern part of Kerala State India. Following loss of domestic fowls and suspecting toddy cats a trap was placed and this unidentified animal was trapped. No ...
0
votes
1answer
31 views

How well do Eppendorf cups seal?

I am considering to send some samples overseas in Eppendorf cups. They are the standard plastic cups of 1 to 2 mL capacity. Of course they may tip over during their journey and I'd like to know how ...
1
vote
0answers
35 views

Why is sex pleasure giving activity but not childbirth?

Asking from an evolutionary viewpoint. Sex is a pleasure giving activity so that it encourages reproduction and spreading of genes. But childbirth ,the most crucial part of the reproductive process, ...
5
votes
2answers
113 views

Is it possible to have different genes in different parts of our body?

I want to understand genetic mutation specially in the context of multicellular organisms like humans. I studied biology only till high school and I can’t fully understand wikipedia pages on this ...
4
votes
1answer
75 views

Can the human body create glucose out of fat?

I read conflicting views about whether or not the human body can create glucose out of fat. Can it?

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