0
votes
1answer
33 views

Why does my anti-ubiquitin antibody visualization not work on my PAGE gel?

I am using 2D gel electrophoresis to visualize polyubiquitinated proteins. However, while I can see actin and heat shock protein using when appropriate antibodies, I cannot visualize them using ...
1
vote
1answer
7 views

Beetle with long, slender iridescent green body and orange legs

I recently saw this beetle while on a trip in central Texas, USA (in a rural area just before sundown). The body was about 2-3 inches long (compare to nearby chair leg and floorboards) and had a long ...
1
vote
2answers
98 views

What is a substance that is safe to eat, not absorbed and not metabolized?

I would like to know whether there are substances that are safe for humans to consume, are not absorbed and do not interact with any digestive process. So, food is disqualified because although it ...
1
vote
0answers
9 views

How many animal species are larger than humans?

I sometimes think about just how huge humans are in comparison to most animals. Even compared to a squirrel, which is really a pretty large organism in the grand scheme of things, humans are hulking ...
1
vote
1answer
44 views

What concentration of table salt is needed to kill bacteria in sweaty gym clothes?

My gym clothes retain an unpleasant musk even after washing them with warm water and detergent. I've experimented with soaking my gym clothes in salty water before washing them. My hypothesis is that ...
4
votes
1answer
49 views

What is the scientific name of this evergreen plant?

This plant is found in India. It is an evergreen plant. Would love to know the scientific name.
0
votes
0answers
6 views

Choosing the best assay for my experiment?

Which of the following assays can be used to determine whether cell death specifically due to apoptosis has occurred in a given cell sample? Choose all that apply. 1) WST-1 Assay 2) Caspase ...
8
votes
0answers
92 views

Biopython - Big Discrepancy Calculating RNA melting Temperature over Literature

I experience big discrepancies when calculating melting temperature of RNA 7-mers with Biopython over values generated by a popular algorithm. I tried the nearest neighbour algorithm with RNA and ...
8
votes
5answers
241 views

Theoretically, is domestication of (virtually) any animal possible?

Looking at ones that manage well alongside us human animals, such as dogs and cats, we see that this is possible for evolved, distant animals to have heritable, preferable traits around people. ...
8
votes
2answers
164 views

Do invasive species cause long-term damage to ecosystems they invade?

Growing up in the U.S., I was warned at various times of the dire consequences of a variety of introduced pests (usually insects). Japanese beetles, gypsy moths, and most recently the brown ...
2
votes
4answers
114 views

Can connections between neurons be weakened?

Connections between neurons are said (by Wikipedia) to be strengthened as part of learning - can they also be weakened (below the original level)? I understand the concept of the connections ...
7
votes
2answers
513 views

Linear and Circular DNA

So school has taught us that eukaryotes have DNA that is linear and inside of a membrane - called the nucleus. And that prokaryotes have circular DNA that is free floating inside of the cell. We ...
2
votes
0answers
40 views

What is the name of this bird?

Does anyone know the name of the bird in the pictures? It was found in the south of Spain in the month of June. (it is certainly neither a house sparrow nor a swallow nor a robin). Thanks for the ...
2
votes
0answers
18 views

Is perceived time compressed or expanded while dreaming? (or does it match reality) [on hold]

I often dream long extended dreams. Sometimes I wonder if the timeline of the story of my dream occurs compressed, in a moment, or other times extended slowly over a long duration. Is there any way ...
5
votes
2answers
98 views

Why does the human brain not overfit when training at some task?

One of the problems that occur during (artificial) neural network training is called overfitting. The error on the training set is driven to a very small value, but when new data is presented to ...
7
votes
1answer
93 views

What is the mechanism behind tinnitus?

I seem to have come across two contrasting explanations for tinnitus induced by loud noises- i.e. damage to the hair cells in the cochlea. On the one hand, I have read that damage to the hair cells ...
-2
votes
0answers
35 views

Buccal vaccine for viral infection of MALT

What would be the effect of a daily (repeated for several months) buccal or sublingual vaccine in the form of a deactivated virus - say HSV1 - to a person already infected with it? Whilst also ...
0
votes
0answers
16 views

Design arbitrary degenerate primers (with non-binding criteria)

I would like to design a number of arbitrary degenerate primers (primers with variable bases, e.g. NGATWGCTSATNGC) for a TAIL-PCR. I would like to be able to ...
2
votes
1answer
39 views

(How) Does the human digestive system process petroleum jelly, aka petrolatum?

Does the human digestive system processes petroleum jelly? In other words, does it pass through the digestive system without any interaction, or is it absorbed and/or metabolized?
6
votes
1answer
250 views

How bad is ethidium bromide in your plasmid for downstream applications?

I want to transfect Adipose Derived stem cells using a clone of my own. My problem is that I get a lot of contamination (not only genomic, but due to the nature of the technique, I get undesired ...
0
votes
1answer
38 views

Which ORF will be translated

I've a question about ORF and translation into protein. Say I have an RNA transcript that contains two ORFs, one in phase 1 ; one in phase2 ; as: ORF number 1 in reading frame 1 on the direct ...
16
votes
3answers
990 views

Why does the face turn pale in dangerous situations?

I know what the effects are of a dangerous situation on the brain, i.e., an activation of the hypothalamic-pituitary-adrenal (HPA) axis which eventually results in an increased heart rate and elevated ...
7
votes
2answers
1k views

Is this a crayfish hole? or wasp nest?

I'm suspicious that it might be crayfish, which I'd think was insane (I still kind of do), if I hadn't found a dead one around my house earlier this summer. Is that definitely what it is, or do I ...
-2
votes
0answers
21 views

Plankton growth as a function of temperature [on hold]

I have a data set like folow Time-Temperature(0C)-Species_1(individuals) 01-T1-S1 02-T2-S2 n-Tn-Sn Now I want to caculate growth of species_1 as a fuction of temperature. How I can do that from the ...
21
votes
1answer
3k views

What is it that creates that wonderful smell after a fresh rain?

I've heard that the wonderful smell of a fresh rain is actually chemicals released from the trees and grass and other plants. What is the process that allows these chemicals to be released? What ...
4
votes
2answers
367 views

Why doesn't human consumption of plants cause infectious diseases the way consumption of animals can?

At first I want to clarify that I do know that there are naturally poisonous plants and plants that can hold radioactive stuff or toxic material from the environment. What I mean by "diseases" (I ...
2
votes
2answers
49 views

How do researchers map root systems of plants?

For example, here is a picture of the root systems for some prairie grasses: (Click image for higher-resolution PDF) from: http://www.conservationresearchinstitute.org/educational-offerings.html How ...
3
votes
1answer
110 views

Enzyme Assay - pectinase

During assaying an enzyme at high temperature, the substrate (Pectin) is degraded by the high temperature rather than by enzyme, so, how can I minimize degradation of the substrate by the temperature? ...
0
votes
2answers
91 views

Why a striped baby is not the result of a white and a black parent? [on hold]

Someone asked me: If a striped cat is the result of a white and a black cat, why the result of a white woman and a black man is not a striped child? I know the answer. I also know that the ...
0
votes
0answers
25 views

What are the effects of diuresis and limited water intake? [on hold]

What are the effects on the kidney when taking a loop diuretic and drinking too little water? Loop diuretics like furosemide cause elimination of water by inhibiting water reabsorption in the nephron ...
4
votes
1answer
35 views

Forward versus backward numerical simulations in population genetics

My question is closely related to this post. There are a number of existing platforms to perform numerical individual-based simulation in populations genetics. An almost exhaustive list of such ...
0
votes
1answer
21 views

What does the “cov” mean in a velvet assembler generated contig name?

A exmaple of a contig name generated by velvet assembler: NODE_127_length_39203_cov_244.873016 What does cov_244.873016 mean? ...
17
votes
6answers
3k views

If a trait would be advantageous to an organism, why hasn't it evolved?

If a trait would be advantageous to an organism then why hasn't it evolved yet? Conversely, if a trait is not advantageous or mildly disadvantageous, why does it exist? In other words why does ...
4
votes
1answer
58 views

How are smell and taste in fish differentiated?

The senses of taste and smell in different fish classes are described as two distinct senses; smell is mediated by the nasal openings, and taste by epithelial taste buds. They are both forms of ...
1
vote
1answer
58 views

Are there any arboreal mammals which eat leeches?

Are there any tree-dwelling mammals which might be found taking a living leech and eating it? (And do any hollow-dwelling birds of prey eat leeches?)
4
votes
1answer
63 views

Identify bee in photo (found in Utah, USA)

A friend took this photo of a bee in his back yard in central Utah (© Jon Mott, CC-BY 3.0). I have no formal training in systematics or entomology, so to me everything looks like the bees' poster ...
1
vote
2answers
22 views

Autotrophic and heterotrophic organisms in anaerobic condition

Can both heterotrophic and autotrophic organism survive in anaerobic conditions? If an autotrophic organism does not produce carbon dioxide but requires oxygen, it should not be present in anaerobic ...
0
votes
0answers
12 views

Upcoming advancements in bio technology [on hold]

What are some upcoming advancements in biotechnology which have a drastic impact on life of common man. For example in mobile technology we saw drastic advancement in mobile technology like touch ...
3
votes
1answer
205 views

How does temperature influence the rate of protein degradation?

For computer modeling purposes, I am looking for some referenced quantitative measurements of the effect(s) of temperature on biochemical reactions. Question In particular, my question is: How does ...
1
vote
1answer
34 views

How do I find the sequence of the phage BxB1 gene?

Specifically, I'm trying to find the sequence of BxB1 and its att sites. I have success finding the att site sequences, but I'm having a bit more of an issue with finding the sequence of the actual ...
14
votes
1answer
895 views

What is this droplet of liquid that comes out of a mosquito?

I have been looking at pictures of different species of mosquito and several times I came across pictures where the mosquito seems to be secreting some kind of fluid. In the pictures one can clearly ...
-1
votes
0answers
22 views

Why haven't humans 'evolved away' aging? [duplicate]

Here's a weird question: why haven't we evolved to live longer, as opposed to living longer through healthcare etc.? It makes sense that if a person lives longer, they have more offspring, ergo in the ...
1
vote
0answers
11 views

Application of Latin names for Taxonomy

The current taxonomy has family names like Canidae and Felidae. But Felidae has sub families like Felinae and Pantherinae. Roaring cats and non-roaring cats. My question is based on the fact that we ...
5
votes
1answer
43 views

Does the anatomy of our hand result in (computer) mouse-accuracy that varies by direction of movement?

First off, I apologize if this isn't the right stackexchange sub-site for asking thsi question. I was torn as to where I should post this question: it could plausibly belong in Biology, User ...
5
votes
0answers
29 views

What have the L-systems (Lindenmayer systems) been actually used for in biology?

Wikipedia: An L-system or Lindenmayer system is a parallel rewriting system and a type of formal grammar. L-systems were introduced and developed in 1968 by Aristid Lindenmayer, a Hungarian ...
-1
votes
0answers
43 views

Do I still have a chance of growing taller? [on hold]

I'm 20 and 5'6". My dad is 6' and my mom is 5'7". I don't believe I went through puberty abnormally early or late. My back molars still have not grown in, and my beard hasn't filled in completely (no ...
4
votes
1answer
86 views

How do steroids travel in the bloodstream?

Cholesterol, which is hydrophobic, cannot simply travel in the bloodstream, and instead is enclosed in lipoprotein (LDL, HDL). Steroids, which are derived from cholesterol, are also hydrophobic ...
0
votes
2answers
73 views

Programmatic way to differentiate homodimers from heterodimers?

For pdb files is there a simple programmatic way to determine if the file is made up of homodimers or heterodimers. I have many files so an online tool or some ...
3
votes
1answer
36 views

How does tearing work?

I found the question about tear gas very interesting. Shigeta says there that crying helps the cells of the eye to wash themselves clear of the toxic components in the tear gas. Does non-emotional ...
3
votes
0answers
44 views

Why do some medicines induce sleep? [on hold]

I have seen lot of people sleep or say that they are feeling sleepy after taking some medicines. Why? I mean, if a medicine acts on some cell of the body because that cell has got its receptors and ...

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