0
votes
0answers
2 views

E Coli + pUC57 plasmid: Promoter choice in commercial gene synthesis

I am trying to use Genscript's de novo gene synthesis service and one part confuses me: Where do I pick the choice of promoter? Or is the promoter choice automatic based on my plasmid choice? e.g. ...
0
votes
1answer
12 views

Can't antibiotics destroy colony of bacteria sometimes?

If we take appropriate antibiotics, it can usually destroy certain bacteria. But if bacteria form a firm colony, can't it destroy colony of bacteria sometimes? or doesn't it matter much?
-1
votes
0answers
8 views

Do any Eukaryotic organism have prokaryotic cells in their body?

I am asking this question, because of this question. Is there any Eukaryotic multicellular organism that has prokaryotic cells in their body? Alternatively is there any Eukaryotic unicellular ...
2
votes
2answers
47 views

Diffusion coefficient of cells in blood?

What's the diffusion coefficient of white cells in blood? Is it well defined, or are cells too large and few as to be treated as particles in this context? P.S. I have tried to look this up, but what ...
11
votes
1answer
146 views

Unilateral damage to vagus nerve

Context: The vagus nerves supply the neck viscera, heart, lungs and gastrointestinal tract. They join around the oesophagus to form the oesophageal plexus. Question: Would damage to one vagus nerve ...
0
votes
1answer
24 views

Parameters of Variant calling analysis

What is the good or stringent parameter for variant calling. At present using the DP > 10 and Q > 30 for Variant calling. Is it ok.? Thanks
-1
votes
0answers
14 views

RNA-seq: Controlling FDR in multiple contrasts [migrated]

I'm working on a RNA-seq analysis project and I have come across a statistics problem. I have 3 groups and I need to look for differential expression for each pairwise comparison. Do I control the ...
3
votes
1answer
21 views

Mesoderm vs Mesenchyme- what's the difference?

My embryology textbook states that mesoderm can exist as Mesenchyme or Epithelium. I'm not sure what 'epithelium' means. Here is a quote for context: Somatic, splanchnic, and somite mesoderm can ...
1
vote
0answers
34 views

Fish and microplastic particles

How does exposure of fish to microplastic particles decrease egg-hatching and give young fish a craving for them? From Garbage in, garbage out: marine plastic 2016-06-24 | Espresso There is more ...
2
votes
1answer
34 views

Identification of a spider from my basement

It's a little over 1" long including legs, and I found it in my basement in Pennsylvania. My guess is a funnel weaver, which would be nice since then I can free it (right?) from the tin can it's ...
1
vote
0answers
23 views

Can a firefly control its glow?

I've just seen this animation: I always thought the firefly glow is ongoing slow chemical reaction, like in glowsticks though through different chemicals. As such it seems impossible to control ...
4
votes
3answers
7k views

Why can't ciliary muscles in the human eye relax like other muscles?

I had laser eye surgery a decade ago, but in recent years my eyesight has become significantly myopic. I consulted an ophthalmologist to see if this was eye strain because I work at computers a lot, ...
1
vote
1answer
23 views

Why does uncompetitive inhibition decrease the Michaelis constant?

I can't seem to find a good resource online that clearly outlines the difference between an uncompetitive, noncompetitive, and mixed inhibitor (I understand competitive inhibitor though). More ...
2
votes
2answers
76 views

How much genomic variation one usually find within a given bacterial species?

If I find the exact starting position (say 1152471) of the coding sequence of a given gene in the genome of a bacterium, is the genome of the bacterium in general stable enough so that I can expect to ...
2
votes
2answers
4k views

Are mature erythrocytes prokaryotic?

Mature mammalian erythrocytes have all the characteristics of a eukaryotic cell except that they don't have a nucleus, they don't have any cell organelles. Does this mean that erythrocytes are ...
-1
votes
1answer
13 views

How can i get only the upstream and downstream genes from chip-seq peak files

I used R and i wrote a script that analyzes peak files from ChiP-seq experiments from ENCODE. I am new to this field, i want to find only the upstream and downstream genes because i want to insert ...
-1
votes
1answer
22 views

what percent of DNA consider to be “junk”?

Does 98% of a whole human genome observe to be "junk" or 98% of each single human DNA observe to be "junk"or "non protein coding region"?
0
votes
1answer
13 views

Are different eye colors indicative of chimerism?

If an animal or person has different colored eyes, does that mean they are a chimera?
0
votes
1answer
61 views

Interpretation of faint band for Linear Array HPV Genotyping Test

After having used the test for research it would appear as if it is very specific. What is one to make of bands that are there but faint? Or what if there are two bands and one is big and bold and the ...
-1
votes
0answers
5 views

Testing for Endospores Using Heat Method

I am trying to test if a new bacillus isolate is in an endospore state. I know there is a staining method of testing this. However, my professor requests I use a "heat method". The idea is that if you ...
-1
votes
0answers
10 views

How does the brain develop different parts?

The mammalian brain contains many parts which perform specialized functions. However, the actual mechanical makeup (neurons) are mostly homogenous throughout the brain. After a brain achieves ...
2
votes
1answer
195 views

What do the letters 'p' and 'c' mean in mutation descriptions?

Example: NM_000525.3(KCNJ11):c. 67A>G ( p. Lys23Glu) AND Permanent neonatal diabetes mellitus Does "p" stand for "polymorphism"? What does the letter "c" stand for?
-1
votes
0answers
15 views

Flora of Camelot [on hold]

In the musical "Camelot", Lerner and Loewe describe the idyllic weather of that mythic land in part this way The rain may never fall till after sundown ... By nine p.m. the moonlight ...
1
vote
1answer
61 views

Nomenclature for mutations at specific nucleotide positions

I'm sure this is a basic question, but I couldn't find an answer anywhere. Let's say I'm provided with the location of a nucleotide as location as FGFR3:c.1843-35A>T. What does "1843-35" mean?
-2
votes
1answer
65 views

how did the domestic dog evolve and was the role humans played in its evolution justified? [closed]

Humans have been selectively breeding dogs for a long time. Why do we do it? What are the positive and negative impacts of selective breeding and why do they occur? I am writing an independent ...
-1
votes
0answers
12 views

Validation of a plasmid detected by DNA sequencing

We detected a 50 kb sequence of plasmid apart from the chromosomal sequence by whole genome sequencing. We are interested in wet lab validation of this plasmid for publishing. Even though wet lab ...
1
vote
1answer
27 views

Name of dominant dinosaur

I seem to remember reading some time ago about a dinosaur appearing very prominently in the fossil record, with continent-wise distribution. The layer is known specifically for having so many fossils ...
17
votes
3answers
4k views

What causes a 'stuffy' or 'runny' nose when you have a cold?

When humans get the common cold, a common symptom is a stuffy or runny nose. Is that the body's immune response or is that the virus's doing?
0
votes
2answers
480 views

Convert gene name to uniprot ID

i have a list of gene name in a file CHRNB2 EGR2 GCK KRT14 LMNA FGF3 TK2 ABCC8 How can I map them to uniprot ID ? P.S I tried Uniprot "ID mapping" (from-"GENEID"...
3
votes
1answer
38 views

What are tail currents?

This is a voltage clamp on an ion channel. When the voltage is stopped there is a still a current at the end (the tail current). I understand that the gates aren't completely closed because of which ...
1
vote
1answer
45 views

How to batch convert gene names to protein IDs in Uniprot?

I have a list of >1000 "ordered locus names" from the STRING database (also called gene numbers, ORF numbers, or CDS numbers by uniprot). They're in the form of 'b0014', 'b0015', etc. I'd like to ...
3
votes
2answers
39 views

How should “the human genome” be interpreted?

What does sequencing "the human genome" mean? Don't individuals have unique genomes?
1
vote
1answer
35 views

No genes' ID in peak annotations during ChiP-Seq with ChIPpeakAnno R package

I am trying to perfom Chip-Seq Analysis which is greatly described in that conversation: introduction to Chip Seq . The same as autor of mentioned question I am a student of Applied Mathematics and I ...
0
votes
0answers
12 views

Are apples really better for the immune system than most fruit? [migrated]

"An apple a day keeps the doctor away", the saying goes, implying that apples are somehow especially good for the immune system. But does this idiom have any validity to it? Are apples high in ...
17
votes
1answer
1k views

Can we taste electrons?

Why does licking a 9-Volt battery elicits a taste sensation? Can taste be stimulated by electrical stimulation?
0
votes
1answer
233 views

Earliest and latest onset of Huntington's Disease(Chorea)?

Huntington's disease can hit at any age, although it tends to hit middleaged people most often. What is the youngest and oldest person that has exhibited Huntington's? Clarification: By oldest I mean ...
18
votes
1answer
796 views

Additive genetic variance components from LMER in R

I've set up some dummy data in R which makes 40 genetically related lines, they are all siblings within a line so are genetically related by a factor of ½ thus additive genetic variance should be ...
4
votes
1answer
72 views

Are all genetic instructions in DNA?

Wiki says DNA is a molecule that carries most of the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses. What ...
1
vote
1answer
84 views

What does NaOH do for the plating of Bacillus subtilis?

A book called Molecular Biological Methods for Bacillus called for plating Bacillus subtilis cells on LB-agar plates with the following components: A general-purpose medium containing per litre: ...
1
vote
1answer
11 views

How are constitutive enzymes regulated?

I found that inducible enzymes can be regulated by many ways, but I cannot find how constitutive enzymes are regulated...
1
vote
1answer
20 views

Mechanism by which water flows through xylem

I was doing a Cambridge iGCSE past paper when I came across the question: Describe the mechanism by which water flows through the xylem I thought the correct answer would revolve around the ...
-1
votes
0answers
16 views

do fishes come to die in beaches? [on hold]

Afternoon I found a fish in a beach, it seems that it kept moving, i was not injured but suddenly stop moving and 'died' so my question is do fishes sometimes come to day at beaches ?? or what could ...
2
votes
1answer
74 views

Ancestral Allele and Hapmap

I notice on dbSNP rs6352 has an ancestral allele of G - http://www.ncbi.nlm.nih.gov/SNP/snp_ref.cgi?rs=rs6352 The hapmap in humans for this allele is very rare, the homozygous version practically not ...
1
vote
2answers
36 views

What is meant by topographic barrier?

There are three types of barriers, physical , climatic and biological barriers that can prevent the dispersal of organisms. Topographic barrier falls in the category of physical barrier but exactly ...
0
votes
2answers
37 views

Predator prey interaction [on hold]

I went through a line in my textbook which read: "But for predators, prey species could achieve very high population densities and cause ecosystem instability." I was not able to understand the ...
-5
votes
0answers
27 views

How to wash hands properly? [on hold]

I have heard from my childhood that you need to wash your hands or else you will be sick. Is this true? By the way what do you mean by this 'wash hands' (or) How to wash hands properly?
15
votes
3answers
5k views

Are there any motile plants?

There are numerous examples of sessile animals (sponges, barnacles etc.) but are there any examples of motile plants? If not, why not? Surely mobility would have conferred an evolutionary advantage to ...
0
votes
0answers
28 views

Forgot to cool slides before washing

I just finished an immunofluorescence experiment and I'm wondering what went wrong. The tissues seem dimmer than they should be. One mistake I made was: I completed the antigen retrieval step, in a ...
0
votes
1answer
71 views

Does RNA serve as the genetic material of some plants and animals along with viruses?

My teacher mentioned that some plants and animals have RNA as genetic material but did not substantiate his statement with examples. I'm wondering if it is so? I have searched the internet but have ...

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