0
votes
0answers
3 views

Why does allele linkage cause disproportionate phenotypic ratios?

I was learning about allele linkage in Biology class and I got confused by the resultant phenotypic ratios. Say you have a dihybrid cross BbGg x BbGg. The Punnett square would look like the ...
0
votes
0answers
3 views

Understanding conclusions of the fact that functional regions of genes are under negative selection?

I am reading in notes for a comparative annotation lecture that : "Fact 1" : all DNA is subject to mutations "Fact 2: most functional regions are under negative selection(ie., mutations are often ...
1
vote
1answer
24 views

Proving time travel

Consider this answer to an interesting question. It's not possible to prove completely that someone is the descendant of someone else, there's always a nonzero probability that the results are a ...
2
votes
0answers
9 views

Expected time for a neutral allele to reach a frequency of $p_1$ when starting at frequency $p_0$

Kimura and Ohta (1968) show that the expected time for a neutral allele to reach fixation (given that it will reach fixation) is $$\bar t(p_0)=-4N\left(\frac{1-p_0}{p_0}\right)ln(1-p_0)$$ , where ...
3
votes
0answers
15 views

Will a store bought cabbage kill my Pieris brassicaes?

About 4 days ago I found a large amount of caterpillars (Pieris brassicae) in my radishes, I took them aside for seeing how they grow and I am out of radishes to give them. I bought a cabbage in the ...
2
votes
2answers
22 views

Any good website/book to understand protein folding and enzymes?

I'm looking for a good, understandable and simple explanation about protein folding, mechanisms and function, and their relationship with enzymes. I understand that the protein is a polypeptidic ...
-2
votes
0answers
15 views

Does the age of marriage have anything to do with our physiology? [on hold]

In the case of girls, it's guessable that their bodies may not have grown strong enough to hold babies, so age of their marriage is set at 18. But what's the reason for boys' age of marriage to be 21? ...
5
votes
1answer
16 views

Behavioral change in dolphins during different hemispheres activity periods?

It is known that dolphins never sleep in the sense that we do, instead they have one hemisphere of the brain sleeping while the other one is awake. I wonder, do they show observable changes in ...
1
vote
0answers
11 views

Incremental denaturation of protein mixtures

When a protein solution is heated above the denaturation temperature, it seems that denaturation does not happen at the time the temperature is reached, but it takes some time. I assumed that the ...
-1
votes
0answers
21 views

For aerobic organism, is oxygen used purely for cellular respiration? [on hold]

I learned about cellular respiration today and it vastly changed my perception of breathing I had since I was small. In fact, I think it would change the perception of anyone who wasn't familiar with ...
0
votes
0answers
18 views

What R packages and software to use for multiple alignment and SNP calling on WGS .gb files?

What R packages and software to use for multiple alignment and SNP calling on WGS (Weighted Sequence Graph) .gb (genebank) files? I have tried Mugsy, DNA Star, ...
2
votes
0answers
11 views

How to quantify the Abundance of a particular gene in Bacteria and Archaea?

For 16s rDNA metagenomic analysis, do I need to do the following steps? DNA extraction-> Purification-> PCR amplification-> Clone -> Pick every single colony-> PCR amplify -> Sequence -> ...
3
votes
2answers
67 views

Change solvent of bacterial solution

I have to perform an RNA extraction that should start with 900 uL of 2%SDS bacterial solution. The problem is that the sample is in 900 uL of PBS. I thought about centrifugation of the sample at ...
2
votes
1answer
19 views

Why proteins are not visible on my membrane after ponceau staining?

I have a problem in western blot that I can't resolve by myself. When I am use to add 100 microgram of proteins for each sample but after running and transfer its impossible to me to see bands of ...
0
votes
2answers
54 views

Horns in animals and birds [on hold]

Are there any carnivores with horns (whether extinct or currently alive)? Do any flying creatures have horns? By horns I mean horn like structure.
3
votes
0answers
26 views

Simplified molecular-input line-entry system

This may be a bit abrupt/vague question, but I didn't know how to proceed the search to get the answer. What are the properties of a 3D biomolecule which gets ignored when a 3D biomolecule is ...
0
votes
0answers
23 views

Which is the 5’ end of the transcript? [on hold]

Given the splice below, how do I find which end is the 5' end? Thank you!! In the following pre-mRNA, the 5’ and 3’ splice sites are highlighted in bold. What is the sequence of the spliced mRNA? ...
2
votes
0answers
11 views

Why introduction of an extra copy of a gene related to pigmentation causes RNA interference in Petunia?

RNAi became famous after Fire and Mello experiment in C.elegans; however, it had been observed before. In the 80's, Jorgensen was trying to increase pigmentation of Petunia flowers by introducing ...
0
votes
0answers
8 views

location of embryo development in angiosperms

What floral organ does the development of the embryos of angiosperms occur in? Is it the ovary? The pollen tube grows down through the style and double fertilization occurs and the embryo begins to ...
4
votes
1answer
20 views

Hand-eye coordination in 3D space

A simple daily event has amazed me and triggered this question. I have read and heard about hand-eye coordination. It quite straightforward when you e.g. want to open a door or play Xbox. In this case ...
0
votes
0answers
14 views

Nutrition in Plants

What is the source of nutritional support for developing microspores and microgametophytes, developing megaspores and megagametophytes, and developing angiosperm embryos?
0
votes
1answer
22 views

Why do home-grown fruits tend to taste better than mass-produced consumer fruits? [on hold]

It is often heard that home-grown fruits and veggies are a lot tastier than the produce found in the supermarket. Although this question may contain a skeptics element in it, it is so often heard ...
4
votes
1answer
32 views

Can the absorption of vitamins be enhanced?

Are food additives like vitamins better absorbed when you mix them among fruits? I heard that secondary substances in fruit promote the absorption.
0
votes
0answers
8 views

Cationic lipid mediated transfection optimization for 150mm dishes

I need to optimize a transfection protocol to transiently express a plasmid encoding a chimera of eyfp attached to the c term of a Golgi apparatus signaling molecule) in hela cells and hepg2 cells ...
0
votes
0answers
12 views

How do BCAA's promote faster muscle recovery?

I am aware that ingesting BCAA's prior, during, and after workouts have an effect on muscle recovery due to their difficulty of metabolism. However, how much more efficiently do they speed up recovery ...
1
vote
1answer
42 views

DNA polymerase in PCR (polymerase chain reaction)

Can the DNA polymerase in PCR (polymerase chain reaction) recognize both DNA and RNA for use them as template? I want to know is it possible if my primers bind to an contaminant RNA and then any DNA ...
2
votes
0answers
19 views

Membrane potential in gram negative bacteria

Does the membrane potential usually quoted for gram negative bacteria (e.g. E. coli) refer to the potential across both membranes? - If yes, then does the potential fall more over the inner or outer ...
1
vote
1answer
85 views

Possible genotypes

If I am blood type B, what are all the possible genotypes that could be expressed by my parents? I think it might be 16 but I was reading online and saw this: Similarly, someone who is blood type ...
2
votes
0answers
19 views

Can encoding of miRNAs in introns not cause dysregulation of gene expression?

For example, consider a gene X which has an intron which encodes a miRNA that downregulates expression of another gene Y. When the gene X is translated, this miRNA will appear as a byproduct of ...
4
votes
1answer
17 views

Is it possible that a woman possessing 1 mutated allele show attenuated color vision deficiency?

Women possess 2 X chromossomes, however, during development, when the embryo has about 32~64 cells, one of these chromosomes is randomly inactivated (in each cell) by a lncRNA named Xist. As a ...
4
votes
1answer
18 views

Are free-nuclear division and endomitosis the same?

As far as I understood it, both are cases of karyokinesis, not followed by cytokinesis.
0
votes
0answers
19 views

Homology ratio comparison between different species

I found the following homology ratio: protein coding genes of the organism with an homolog in mouse / all protein coding genes in the organism I have done this for all protein coding genes between ...
4
votes
1answer
54 views

Can other animals see black differently?

I am not sure if this is the right place for this question, but this is a debate that has been going on between two colleagues for days and I need a resolution because it's driving me crazy. So any ...
-1
votes
0answers
30 views

Why can't we remember something sometimes? [on hold]

We've all had something a time where we "learned" something for school and then forgot it the next day. However, we remember something that happened 3 years ago. Why is our short term memory ...
-2
votes
0answers
40 views

Use genetical modification to produce quantum computer? [on hold]

Is it theoretically possible to use organism modifications in order to make it produce molecules capable of forming Qbits, therefore performing quantum computing. Instead of constructing Qbits ...
1
vote
3answers
50 views

Can bioluminescence be used for cancer or tumor detection? [on hold]

What applications, if any, are there in using bioluminescence to detect cancer or tumors (in vivo)?
3
votes
1answer
39 views

Mathematical models of lineage selection

I'm interested in the concept of lineage selection (Aboitiz, 1991) as an explanation for why traits would be selected for that enhance the rate at which evolution can occur, rather than directly ...
0
votes
0answers
14 views

Steps in making mass/volume concentrations? [duplicate]

[TLDR When making mass/volume concentrations, is the general workflow to make a stock and dilute from it to yield the proper diluted concentrations? Could anyone detail their methodology on the ...
2
votes
1answer
16 views

Is it possible to accurately measure micronutrient levels with the blood volume from a Dermal / Capillary puncture?

I have not been able to find much information beyond what tests are normally done with Capillary puncture sampling, as opposed to what tests are possible. Could we test for a single micronutrient, or ...
3
votes
1answer
34 views

What color was the most recent common ancestor of all swans?

Was the most recent common ancestor of all swans black, or white, or some other color? How do we know, and how certain can we be?
2
votes
2answers
166 views

Using an analytical balance (0.0000 precision) to measure µg (micrograms)?

If i needed to prepare 200µg/ml of proteinase K, and the proteinase K was in a solid powder form, would I have to weight out 200 µg using an analytical balance, and if so, is it possible with a ...
1
vote
0answers
16 views
3
votes
2answers
42 views

Sequence compression method / format

As someone working with sequencing data and sequence files I was wondering whether is there a compression method / format for sequence / read files? Of course one can use general compressors like rar ...
-2
votes
1answer
43 views

How to make µg/ml concentrations of proteinase-K?

How does one prepare concentrations in the mass/volume (weight/volume) form, for substances like nucleic acids or in this case, proteinase? A detailed example would be helpful. I need to prepare ...
5
votes
2answers
46 views

What percentage of a cell's volume is occupied by protein?

I was looking at one of David Goodsell's illustrations of a cell: And it seems to suggest a very crowded picture of the intracellular environment. Just how crowded are cytoplasms? What percentage ...
1
vote
0answers
34 views

Primer Designing [on hold]

I have a basic idea about the requirements in primer designing. Basically my knowledge is limited to theoretical knowledge and have no experience in actual primer designing. And also I have a basic ...
2
votes
1answer
30 views

Is the Klein's bottle a good analogy to the relation between T-tubule and sarcolemma? [on hold]

I am not quite seeing how the T-tubule and sarcolemma is connected. It says that the T-tubule is an "invagination" of the sarcolemma, which is sarcolemma folded from the inside to form a T-tubule Can ...
2
votes
1answer
56 views

Can silverfish climb and produce silk?

I am equally disgusted and fascinated by the "Silverfish" (Lepisma saccharina) that we regularly find in our UK home. I tried searching for information on the internet but they seem poorly understood. ...
2
votes
0answers
37 views

Examples of Vestigial Genes

I consider the existence of a broken GULO gene in humans to be the clearest evidence for human evolution - just what else is it doing there otherwise? Are there any other examples of vestigial genes ...
2
votes
0answers
25 views

Contact Inhibition of Cell Division: Signaling Pathway

The following article refers to contact inhibition of cell division in epithelial cells, specifically MDCK cells: Collective and single cell behavior in epithelial contact inhibition. In their review ...

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