All Questions
0
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0answers
3 views
Endophytic Xylariaceae: diversity and taxonomy inferred from rDNA sequence analyses
"Thailand is considered as one of the areas containing a high percentage of unknown taxa of Xylariaceae (Rogers 2000). In Thailand, several studies on endophytic fungi have been documented, namely, ...
0
votes
0answers
7 views
By what mechanism does taking a shower wake up and energise?
My personal experience is that a warm or hot shower can make me feel good, alert and energised. I've heard others having similar experiences, some mentioning cold shower as a good method to wake up.
...
-2
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0answers
21 views
International standard to prepare an International journal [closed]
Any of you could give the link or reference regarding International standard to prepare an International journal on microbiology field?
0
votes
1answer
32 views
TCGA data, and bioinformatics design questions for SNP/ mirna analysis
It's my first time posting to this forum but was looking for some help on the data aspects of this project.
My tools of choice would be in python/R .
Goal: I'm looking to create a disease specific ...
2
votes
0answers
12 views
How to use mechanical microstrainer to extract tissue proteins from human?
Background:
There are many methods to extract proteins form human tissues out there. The majority of them use an extraction buffer containing variable concentrations of detergents and protease ...
0
votes
0answers
28 views
Wheat anthracnose outbreaks caused by Colletotrichum cereale in the USA
"Wheat anthracnose outbreaks caused by C. cereale were problematic during the 1940s in the USA, but presently do not limit production of this crop, despite the fact that the fungus still inhabits ...
0
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0answers
18 views
Is it “extreme” or “large” diversity for endophytic microorganism subject? [migrated]
Do I need to substitute the word extreme to large in this text?
Endophytic fungi are molds that are found in the healthy plant tissues. Endophytic fungi is mold that exists in the plant tissue ...
0
votes
1answer
33 views
What kind of arthropod/animal is this?
Can anyone identify this strange creature? I live in a very urban part of Montreal, Canada. It wasn't moving (seemed dead) and although the image is huge, it wasn't as big as it seems (a couple of ...
1
vote
1answer
27 views
How difficult is to renature a protein?
I know of Anfinsen's experiments and I'm aware that some denatured enzymes may regain their lost activity through the removal of the denaturant agent. What I'm unaware of is how rare is it for a ...
-1
votes
1answer
51 views
What determines a journal's 'impact factor'? [closed]
This isn't directly linked to biology, but it does seem to crop up as being the driving force when deciding which journal to apply for when attempting to publish an article.
Journals like Cell, ...
0
votes
1answer
34 views
Has medical progress stopped human evolution?
We can cure many diseases nowadays, and thus the natural selection is very limited.
Plus, mankind spent the whole beginning of its existence in almost the same hostile environment, and that's where he ...
0
votes
1answer
14 views
What is the disadvantage of having no anticoagulation after pulmonary embolism?
Is chronic anticoagulation always given after pulmonary embolism?
What are the risks or disadvantages
if anticoagulation is not given?
if terminated early?
2
votes
1answer
20 views
By what mechanism is Streptococcus bovis acting as a risk factor for colorectal cancer?
Streptococcus bovis bacteremia/endocarditis is considered a risk factor for colorectal cancer. What pathophysiological mechanism may link the two together?
0
votes
1answer
47 views
Writing a paper
Considering that I am writing a paper for a journal, could the following phrase be understood? or should I put the 'step-by-step' way by giving numbers?
PCR Amplification and sequencing
PCR ...
0
votes
0answers
20 views
Are there plants that excrete poisons into their environments? [duplicate]
Plant growth is very much limited by other plants in its neighbourhood, "stealing" their potential nourishment. So are there any plants that solved this problem by killing its neighbouring plants, ...
0
votes
0answers
7 views
What's seen when stress echocardiography shows ischaemia? Can this happen without angina?
What can be seen on the screen when stress echocardiography suggests ischaemia? Can this happen without chest pain?
1
vote
1answer
29 views
Primer design for Gibson assembly
I'm trying to design a primer for Gibson assembly. My gene of interest is on a plasmid, and I want to copy that gene, and put it into a different plasmid.
I am unsure how to design my primers for ...
2
votes
2answers
73 views
Why do neurones use chemical signalling at synaptic junctions?
Problem. When a neurone fires, it sends an electrical signal that jumps down the axon via the nodes of Ranvier very rapidly. At a synaptic junction, chemical brownian diffusion signalling with ...
2
votes
0answers
15 views
What is the purpose of Y-shaped adapters in Illumina sequencing?
Y adapters different sequences to be annealed to the 5' and 3' ends of each molecule in a library.
The arms of the Y are unique, and the middle part, connected to the DNA fragment, is complementary.
...
0
votes
0answers
14 views
General undergraduate zoology textbook recommendation?
I am very interested in zoology,and I want to study zoology in my spare time.
Any undergraduate level zoology text book recommendation?
2
votes
3answers
41 views
Knockdown of long noncoding RNA (lncRNA) - how is it done?
I don't work at the wet lab and don't know all the details about the knockdown techniques.
My question is:
How lncRNA knockdown is done?
For example - you have lncRNA that is functional in the ...
0
votes
1answer
60 views
Are humans more aggressive during a full moon? [closed]
Is it true that we are more agressive during the night when there is a full moon?
If true, could this be a remnant from times where we needed to hunt for our food and so could see more at night when ...
4
votes
0answers
26 views
What effect do oligosaccharides, like those found in legumes, have on the composition of intestinal flora in humans and if so how so?
I know that intestinal flora metabolising oligosaccharides, like those found in legumes, is the cause for the well known fact that legumes cause flatulence, but does an oligosaccharide-rich intestine ...
0
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0answers
18 views
What material is recommended for autoradiography cassettes when working with 32P?
So, during radiation safety training, much hullabaloo is made regarding how the high energy beta emitted by 32P produces Bremsstrahlung radiation when striking a high Z shielding material. As such, ...
3
votes
1answer
45 views
Why does it hurt more when you touch a nerve directly?
I am not a biologist nor know much about biology (so please explain in layman's terms) however I have always been curious as to why this is.
What causes the difference in pain between touching an ...
2
votes
1answer
26 views
How is transport of glucose into prokaryotic cells different from transport into eukaryotic cells?
I was reading page 92 of Fundamentals of Microbiology, 4th edition, which states
In facilitated diffusion, the substance (glucose, for example) to be transported combines with a plasma membrane ...
0
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0answers
24 views
What molecules/elements/compounds are in the cardiovascular system? [closed]
Like in topic, what molecules/elements/compounds are important and inside the cardiovascular system. I'm researching a topic, and it's important for me to know how what you eat affects the ...
1
vote
0answers
19 views
What are ways to compare the “costs” and “gains” of food production systems?
I'm looking for methodologies to compare the costs a food production requires with the gains it delivers (not in economic terms).
I'm aware of energy returned on investment. Are there other methods ...
4
votes
0answers
20 views
How are our senses dimmed during sleep?
Our senses are not as sensitive during sleep compared to wake. There is an arousal threshold during sleep that only when it is overcome will it wake a person up. My question is how is this arousal ...
5
votes
1answer
64 views
Why aren't organelles considered alive?
I'm taking a 3-week Bio I summer course, and my textbook says the cell is the smallest/basic unit of life. I asked my professor why organelles aren't a living unit, and her reply was that they aren't ...
0
votes
0answers
22 views
In which blood vessels are these nutrients first found?
Some nutrients from the small intestine get absorded into the lymph and then led into the systemic circulation.
In which blood vessels are these nutrients first
found?
The answer should be in the ...
2
votes
1answer
30 views
Why is the blood pressure in the superior vena cava during inhalation less than during the exhalation?
For further investigation into the progress of the blood pressure, we will measure a patient's blood pressure in the superior vena cava measured during inhalation and during an exhalation. His heart ...
3
votes
1answer
35 views
What are some examples of scaling laws in biology?
I've seen that metabolic rate scales logarithmically as function of mass for many animals over an extremely large span of parameters. What other scaling laws exist at the individual level?
1
vote
1answer
43 views
Why is a slow worm not considered a snake?
Slow worms are considered lizards as opposed to snakes, both are reptiles. Now I get that there are traits that distinguish them (eye lids, ears ...). But snake species themselves vary already quite a ...
5
votes
1answer
89 views
If Evolution Is In Progress, Why Fight Extinction?
Natural selection is a central dogma of evolution. However, most biologists seem determined to prevent the extinction of the species that have been selected against. Why is this? Preservation of ...
2
votes
0answers
27 views
Why do ants dig surface paths?
A few days ago I observed a corridor built and used by ants that went over a path in fields:
Why do they build it? It seems it doesn't bring any considerable protection from the surrounding ...
0
votes
0answers
18 views
Basic book on Human brain anatomy [closed]
What are some of the best resources on the human brain. Can some one suggest a book that will give details starting from very basics (schools level)?
0
votes
0answers
25 views
Modeller - Convert FASTA to PIR
I am doing a basic modelling following the instructions here. But different target / template sequences.
http://salilab.org/modeller/tutorial/basic.html
Do I need to convert the FASTA to PIR with a ...
1
vote
1answer
47 views
Why is Sanger sequencing inferior for detecting SNPs in cancer cells?
I am familiar with Sanger sequencing, but at the level of an undergraduate. A lecturer of mine tried to describe Sanger sequencing as losing the sequence information in noise when used to detect ...
0
votes
1answer
27 views
SNPs mapping into protein
Starting a new project on protein-protein interactions and SNP analysis tool development. I would like to ask how does SNPs is mapped into protein? What does mapping means?
2
votes
1answer
33 views
Storing Apples at 30F?
This article about storing apples for the winter says:
You do not want the apples' temperature to fall below 30°F (-1.1°C), however,
because that will make them freeze and turn to mush when ...
4
votes
1answer
43 views
Breeding laboratory mice with cancer; how does this work?
It has recently been brought to my attention that live laboratory mice with specific cancer strains can be readily purchased for medical research. For example, the Jackson Laboratory sells mice with ...
1
vote
1answer
20 views
What is solid-phase bridge amplification?
During Illumina sequencing there is a step called bridge amplification by which DNA is amplified by isothermal enzymes. What is this stage, and how does it work?
3
votes
1answer
43 views
What's the difference between shotgun sequencing and clone based sequencing?
What are the main differences between shotgun sequencing and clone based sequencing?
5
votes
1answer
43 views
Does the human ear adapt to noise levels?
I have noticed lately that if I go to sleep, when I have my radio running, it is on lowest volume, I still consider it kind of loud.
In the morning, when being on the edge of waking up, I hear the ...
1
vote
1answer
44 views
“Oblong to Allantoids” … Is it valid?
I read on the text book:
The characteristic of Dinemasporium are: superficial, cupulate to Discoid conidiomata with brown satae, Phialid conidiogenous cells that give rise to hyaline, oblong to ...
1
vote
1answer
21 views
By what mechanism does elevated homocysteine level accelerate thrombin formation?
It has been stated that elevated homocysteine level accelerates thrombin formation. By what mechanism does this happen?
1
vote
0answers
15 views
By what mechanism does elevated homocysteine level cause endothelial dysfunction and damage?
It has been stated that elevated homocysteine level causes endothelial dysfunction and damage. By what mechanism does this happen?
0
votes
1answer
40 views
Does anyone know what marsupial this is? [closed]
I know what this is a marsupial, but i'm not quite sure which one. Any thoughts?
1
vote
0answers
29 views
How can I view modENCODE data faster?
I am trying to view several data tracks in the modENCODE GBrowse genomic browser. However, the site is so slow, it is practically unworkable. Is there a faster way to explore the data?
