-1
votes
0answers
3 views

RNA-seq: Controlling FDR in multiple contrasts

I'm working on a RNA-seq analysis project and I have come across a statistics problem. I have 3 groups and I need to look for differential expression for each pairwise comparison. Do I control the ...
-1
votes
0answers
4 views

Testing for Endospores Using Heat Method

I am trying to test if a new bacillus isolate is in an endospore state. I know there is a staining method of testing this. However, my professor requests I use a "heat method". The idea is that if you ...
-1
votes
0answers
8 views

How does the brain develop different parts?

The mammalian brain contains many parts which perform specialized functions. However, the actual mechanical makeup (neurons) are mostly homogenous throughout the brain. After a brain achieves ...
-1
votes
0answers
14 views

Flora of Camelot [on hold]

In the musical "Camelot", Lerner and Loewe describe the idyllic weather of that mythic land in part this way The rain may never fall till after sundown ... By nine p.m. the moonlight ...
2
votes
1answer
185 views

What do the letters 'p' and 'c' mean in mutation descriptions?

Example: NM_000525.3(KCNJ11):c. 67A>G ( p. Lys23Glu) AND Permanent neonatal diabetes mellitus Does "p" stand for "polymorphism"? What does the letter "c" stand for?
1
vote
0answers
20 views

Can a firefly control its glow?

I've just seen this animation: I always thought the firefly glow is ongoing slow chemical reaction, like in glowsticks though through different chemicals. As such it seems impossible to control ...
-1
votes
0answers
12 views

Validation of a plasmid detected by DNA sequencing

We detected a 50 kb sequence of plasmid apart from the chromosomal sequence by whole genome sequencing. We are interested in wet lab validation of this plasmid for publishing. Even though wet lab ...
2
votes
1answer
30 views

Identification of a spider from my basement

It's a little over 1" long including legs, and I found it in my basement in Pennsylvania. My guess is a funnel weaver, which would be nice since then I can free it (right?) from the tin can it's ...
1
vote
0answers
30 views

Fish and microplastic particles

How does exposure of fish to microplastic particles decrease egg-hatching and give young fish a craving for them? From Garbage in, garbage out: marine plastic 2016-06-24 | Espresso There is more ...
1
vote
1answer
24 views

Name of dominant dinosaur

I seem to remember reading some time ago about a dinosaur appearing very prominently in the fossil record, with continent-wise distribution. The layer is known specifically for having so many fossils ...
0
votes
0answers
14 views

Parameters of Variant calling analysis

What is the good or stringent parameter for variant calling. At present using the DP > 10 and Q > 30 for Variant calling. Is it ok.? Thanks
-1
votes
1answer
13 views

How can i get only the upstream and downstream genes from chip-seq peak files

I used R and i wrote a script that analyzes peak files from ChiP-seq experiments from ENCODE. I am new to this field, i want to find only the upstream and downstream genes because i want to insert ...
0
votes
0answers
12 views

Are apples really better for the immune system than most fruit? [migrated]

"An apple a day keeps the doctor away", the saying goes, implying that apples are somehow especially good for the immune system. But does this idiom have any validity to it? Are apples high in ...
0
votes
1answer
20 views

what percent of DNA consider to be “junk”?

Does 98% of a whole human genome observe to be "junk" or 98% of each single human DNA observe to be "junk"or "non protein coding region"?
0
votes
1answer
11 views

Are different eye colors indicative of chimerism?

If an animal or person has different colored eyes, does that mean they are a chimera?
1
vote
1answer
11 views

How are constitutive enzymes regulated?

I found that inducible enzymes can be regulated by many ways, but I cannot find how constitutive enzymes are regulated...
-1
votes
0answers
16 views

do fishes come to die in beaches? [on hold]

Afternoon I found a fish in a beach, it seems that it kept moving, i was not injured but suddenly stop moving and 'died' so my question is do fishes sometimes come to day at beaches ?? or what could ...
3
votes
2answers
37 views

How should “the human genome” be interpreted?

What does sequencing "the human genome" mean? Don't individuals have unique genomes?
1
vote
1answer
38 views

How to batch convert gene names to protein IDs in Uniprot?

I have a list of >1000 "ordered locus names" from the STRING database (also called gene numbers, ORF numbers, or CDS numbers by uniprot). They're in the form of 'b0014', 'b0015', etc. I'd like to ...
0
votes
0answers
28 views

Forgot to cool slides before washing

I just finished an immunofluorescence experiment and I'm wondering what went wrong. The tissues seem dimmer than they should be. One mistake I made was: I completed the antigen retrieval step, in a ...
1
vote
2answers
36 views

What is meant by topographic barrier?

There are three types of barriers, physical , climatic and biological barriers that can prevent the dispersal of organisms. Topographic barrier falls in the category of physical barrier but exactly ...
-1
votes
1answer
14 views

What is the total estimated biomass of Earth? [on hold]

If possible could you also give the distribution according to species or ecosystem wise such as, aquatic, terrestrial etc.?
4
votes
1answer
72 views

Are all genetic instructions in DNA?

Wiki says DNA is a molecule that carries most of the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses. What ...
-5
votes
0answers
27 views

How to wash hands properly? [on hold]

I have heard from my childhood that you need to wash your hands or else you will be sick. Is this true? By the way what do you mean by this 'wash hands' (or) How to wash hands properly?
0
votes
0answers
7 views

Phase separation of FIB1 Protein in NaCl solution [on hold]

What is the mechanism of phase separation that causes droplets to form in a solution of proteins and NaCl, given the concentration of NaCl that allows this to happen?
0
votes
1answer
27 views

What is the effect of wheat's polyploidy on wheat itself and on us?

According to Wikipedia, "Hexaploid wheats evolved in farmers' fields", and bread wheat is among them. There is also diploid and tetraploid wheat. I guess that hexaploid wheat, besides the obvious ...
0
votes
0answers
20 views

How does plant life obey the constraint that the change in Gibbs free energy in a reaction must be negative?

My understanding is that if a reaction takes place, either entropy of the system must rise, or energy must be released from the system as heat, or both. (Citation: paraphrasing from The Vital Question,...
0
votes
0answers
9 views

Can temporomandibular joint disorder cause sensorineural hearing loss?

Is the pain when walking or running with such a disorder an indicator that hearing loss is occuring or going to occur? Ear pain is considered a symptom for this disorder. Can that be an indicator of ...
0
votes
1answer
19 views

How to put a gene under the control of a regulable promoter?

Suppose I have a hetrologous (eukaryotic, plant) gene sequence that I want to express in E Coli under the control of a regulable promoter (say the lac promoter induced by IPTG) how exactly does one ...
0
votes
0answers
23 views

Can crocodiles live past 155 years of age?

I was at the Teluk Sangat Crocodile Farm ("Crocodile World") in Johor, Malaysia. There were a bunch of crocodiles that were claimed to be 60-80 years old (these were printed signs), one or two that ...
-2
votes
0answers
15 views

Choose the intruder [on hold]

A. Antibody B. Antibiotics C. Micro-tubule D. Cholesterol I couldn't find a link between any of these 3 components to actually find an intruder.
3
votes
1answer
35 views

Is there evidence to suggest that sharks from prehistoric times live in the deep of the oceans?

I've had friends see the boat and giant teeth of a giant shark attack of a boat in Japan. (Teeth larger than a great white). [I can't find anything about this on the Internet]. In addition, this ...
0
votes
0answers
32 views

Stopping the Gag Reflex: How and why does it work?

I was talking to some friends over the weekend, and one of them made a comment about gagging. He said that he read or watched that clenching your thumb with your other fingers (making a tight fist ...
0
votes
1answer
32 views

Deducing protein sequence length from gene DNA sequence length

Is there a standard way to deduce protein sequence length from gene DNA sequence length of the gene coding for it? Naively I had assumed that ...
0
votes
0answers
16 views

RNA interference and the former Petunias experiment >

I'm having problems trying to understand how RNA interference works. From what I understood, in simple terms there is this dsRNA which is fragmented into siRNA by the enzyme called Dicer, and those ...
0
votes
0answers
9 views

Restriction enzyme recognition sites for plasmid libraries

I'm having trouble with the following question: You wish to cleave genomic DNA into fragments average about 2kb for cloning into a plasmid library. Which of the following restriction enzyme ...
3
votes
1answer
57 views

Forgot to vortex antibody before staining

Ugh. Did an immunofluorescence experiment last weekend, forgot to vortex both my primary and my secondary antibody solutions. And my final result looks dimmer than it should be. Is it possible that ...
0
votes
0answers
25 views

Influence of alcohol/hangovers on sex drive [on hold]

I have noticed that whenever I go out and drink alcohol I'll have a much higher desire for sex with my hangover the next day. I'm harder and the sex is better (this is not a troll question btw, I'm ...
1
vote
0answers
20 views

Absorption of nutrients in human beings [on hold]

I know that pulmonary veins carry oxygenated blood from lungs to heart, and pulmonary arteries carry deoxygenated/CO2 rich blood from heart to lungs. Arteries also carry oxygen-rich blood to the heart ...
-1
votes
0answers
28 views

Why people have less hair than other primates? [duplicate]

Well as you can figure out, most primates such as monkeys, gorillas and chimpanzees, have all their body covered with fur. As we know they are evolutional "relatives" to us humans, but we dont have ...
-1
votes
0answers
6 views

How to Study PR intervals as HRV Metrics? [on hold]

I am trying G. Clifford book about ECG Data Analysis 2006. RR intervals are mostly used as HRV metrics. PR intervals not because of low magnitude of detection. I would like to get some samples of such ...
0
votes
2answers
37 views

Predator prey interaction [on hold]

I went through a line in my textbook which read: "But for predators, prey species could achieve very high population densities and cause ecosystem instability." I was not able to understand the ...
4
votes
1answer
41 views

Why is the Pentose Phosphate Pathway so active in erythrocytes?

Is it because glyceraldehyde -3-phosphate (a molecule which when enter glycolysis help produce ATP through substrate level phosphorylation) can be prepared without losing an ATP through this process?
3
votes
1answer
24 views

Could a large constrictor kill a small animal?

According to this article there was a python whose body circumference was 85cm. This means its diameter was 27cm. Would it be possible for this snake to constrict animals that are about the same ...
1
vote
0answers
15 views

O-Group names of Salmonella serotypes?

What liposaccharides are represented by the O-group designations of Salmonalla serovars in the Kauffman-White classification scheme? I mean to ask, how do the numbers in a Sal serovar (1, 9, & 12 ...
1
vote
0answers
12 views

How can I perform a 1:1 sequence-structure alignment for a pdb template dimer and homologous dimer sequence?

I have: 1. A PDB dimer protein structure template of a real solved protein. 2. An unsolved, but highly homologous dimer protein (>90% sequence identity.) How can I use MODELLER to make a theoretical ...
3
votes
0answers
16 views

Names for the degrees for symptoms from drought (or other damage) done to trees

Looking out my window, I see trees suffering from drought. I once knew the name for the degrees visible, but I have forgotten and can't find it on the net. One level was the tree being completely ...
-1
votes
0answers
12 views

Is there a way to convert/export track files (.trc file) generated from Media Cybernetics Image Pro Plus to .mat file type? [on hold]

I am trying to export these files so that they can be used with a matlab based automated behavior classification software JAABA. At the very least I would like to figure out a way to read the format ...
0
votes
1answer
41 views

How does DNA Polymerase make errors? [on hold]

DNA Polymerase inserts the wrong nucleic acid base into DNA at a rate estimated to be between 1 in 107 and 1 in 108. How does it determine which base to match to the template strand, and how does it ...

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