3
votes
1answer
158 views

Pigmentation in Jaundice

My textbook says that the yellow appearance of a jaundiced person is due to the accumulation of bile pigments in the skin. I am unable to understand why these pigments are not deposited in a healthy ...
4
votes
1answer
209 views

Why do some protocols require prewarming a liquid medium before inoculating?

For example, in this protocol for E. coli competent cell preparation, it says: Plate 10 uL E. coli BL21(DE3) cells on a LB-agar plate; incubate overnight (12 hours). Prepare 500 mL SOB medium ...
3
votes
2answers
174 views

What species is this worm? [closed]

I was at the park lying on the grass and its the third time I have seen them, I used to think they were parasites when I was like 7. It is the very small brown worm on the green leaf. It moves by ...
11
votes
1answer
144 views

What limits chromosomal length?

What are the upper and lower limits for chromosome length? Are these limits different in different species or kingdoms? If there is any limit, which cellular or molecular factors are reasons?
2
votes
1answer
92 views

Additive genetic variance $V_A= 2pqa^2$ … what is $a$?

Reading Falconer and Mackay looking at equation $V_A = 2pqa^2$ (equation 8.5, page 126). I am trying to write a clear explanation of how additive genetic variance is defined and calculated, but I ...
7
votes
0answers
239 views

What bacteria shed harvestable energy from root zone organic matter?

I recently heard of a successful effort that harvests energy from soil at plants' bases due to apparently bacterial breakdown of wastes from the plants: Via photosynthesis a plant produces organic ...
-3
votes
1answer
67 views

From where can I download the database created by Hsu et al for MORFs? [closed]

I am working on an independent research project for which I require access to the MORF database created by Hsu et al. From where can I download the database of MORFs of IDPs and their binding partners ...
2
votes
0answers
69 views

Falconer & Mackay population mean calculation & genotypic values

I am reading chapter 7 of Falconer and Mackay where they give a formula to calculate the population mean as a deviation from the heterozygote genotypic value. As an example, imagine a one locus two ...
1
vote
1answer
133 views

How Scientists sequence, assemble and annotate plant genomes? [closed]

I previously read this question and this paper and learned good things about this topic. my current question is that scientists use which tools, algorithms, soft wares... to sequence, assemble and ...
2
votes
2answers
226 views

Cleavage of RNA by restriction enzymes?

Six restriction enzymes discussed in Sequence-specific cleavage of RNA by Type II restriction enzymes (Murray et al.) have the ability to detect and cut RNA strands with that enzyme's recognition ...
6
votes
1answer
174 views

What are the psychedelic effects of (star) anise and how many people are affected?

Backstory: My girlfriend reacts heavily to chocolate and drinks containing stellar anise, in a way that seems comparable to psychedelic drugs. After consuming it, it takes approximately five minutes ...
2
votes
1answer
69 views

What is this plant on the photos?

Found in Kustaankartano, Helsinki, Finland in June 2015. (for closeup view, copy image URL and open in new window and then zoom in)
5
votes
1answer
70 views

The ermine's colour and geographical range

Ermines turn white during the winter, with only the tip of the tail remaining black. However, in the southern regions of the ermine's range it clearly doesn't get so white. Encyclopedia of Life says ...
2
votes
1answer
79 views

Better population models than the logistic equation [closed]

For modelling the population of a single species in a region, the logistic equation is used $$ \frac{dN}{dt} =kN\Big(1-\frac{N}{K}\Big) $$ where $N$ is the number of organisms, $K$ is the carrying ...
1
vote
0answers
700 views

What's the difference between blood value units 'mE/l', 'mU/l', 'mIU/l' and 'mEq/l'?

Wikipedia suggests 'mE/l' is the dutch or german translation (using 'eenheid'/'einheit' for 'unit') for 'mU/l', which can also be written as 'mIU/l'. The article mentions ...
2
votes
1answer
62 views

Can Opioids Attenuate some of the symptoms of Psychosis?

Can Opioids Attenuate some of the symptoms of Psychosis? I ask because there's a dead link on the Wikipedia page that's meant to support the notion that opioids attenuate some of the symptoms of ...
8
votes
1answer
259 views

What is this insect that has invaded my home in Texas?

After the recent floods in central Texas this past weekend I noticed my that bedroom has suddenly been infested with these small insects that have climbed in through the window. Previously, the hole ...
6
votes
1answer
740 views

Does inhaling glue (glue sniffing) reduce appetite?

I have heard from many homeless people that they sniff glue just to reduce appetite, as it comes cheap and is more affordable than actual food. Is this true, and if yes, why? This is totally opposite ...
5
votes
0answers
53 views

Is hunger pushing cognitive ability in humans? [closed]

We have a lot of those questions here that base on personal anecdotes and I try to avoid asking any of those. Still, after skipping breakfast and an extremely effective work day so far I started ...
11
votes
2answers
29k views

Why is SOC medium recommended for transformations?

In pretty much every transformation protocol I've seen SOC medium is used to grow the bacteria for a short while after the tranformation and before plating. I've usually substituted LB medium for ...
3
votes
0answers
163 views

Can I leave BL21(DE3) cells in room temperature?

I am preparing competent cells, and I finished inoculating a single colony in SOB. It has been incubating at 37 degrees Celsius for almost 16 hours since, and it's getting very late where I live. I ...
6
votes
1answer
62 views

Are there medical treatments that were so effective they were never subjected to a randomized control trial?

I'm thinking of things like antibiotics for meningitis or tuberculosis, or thiamine for Wernicke-Korsakoff syndrome. In those cases the magnitude of the treatment effect might have been so great that ...
8
votes
2answers
2k views

Why pick just a single bacterial transformed colony

So after bacteria have been transformed to perhaps grow up a plasmid of interest, why pick only a single bacterial colony from a selective plate for further expansion? I understand that this is to ...
3
votes
4answers
553 views

Identifying the origin of replication of an unannotated *E. coli* plasmid

I have attempted a few searches for a list of origins of replication for plasmids in E. coli, but I was only able to find a list of origins, but not their individual sequences. The available plasmid ...
10
votes
3answers
28k views

Why can't the brain and red blood cells use fuels other than glucose?

The question is rather straight forward: I have always been curious as to why, but cannot find an explanation online. I can imagine that the mechanism is different for each, but why does brain tissue ...
6
votes
1answer
93 views

How many dinosaur genes are in a chicken genome?

I read recently that we can "work back" to a dinosaur by selectively turning off certain genes in chickens, and we were able to create a chicken with a snout instead of a beak. How many dinosaur ...
10
votes
1answer
904 views

Do I not have any alleles from my mom's mom & my dad's dad?

So, I have an XX, & I got an X from my mom, & an X from my dad. My dad would have gotten his only X from his mother, & my mother could have gotten the X I got from her father or mother. ...
3
votes
1answer
94 views

Why do surface mole tunnels follow directly below the electric fenceline

I have electric fencing enclosing five acres, and moles always seem to tunnel in a straight line just under the electric fence - are they following a force field? Can that be?
3
votes
3answers
515 views

Why are there no trees in Texas?

In Texas, there is lot of grassland and many cotton fields, which need a great deal of water. However, I have not seen any forests or areas with many trees. Why are there no forests or heavily-treed ...
8
votes
1answer
561 views

Reason for seeing grid-like patterns when strobing a solid black-white image?

I was trying to do some stimulation for SSVEPs by strobing a solid black square and a solid white square. Weird thing was, I ended up seeing weird grid like patterns that looked like this, always of ...
3
votes
1answer
39 views

How do cold conditions help organ transplants?

Body organs are kept cold in-between explanting them from the donor and implanting them in the new host. How do these cold conditions help organs to stay viable while they haven't fresh blood, energy ...
4
votes
0answers
115 views

Mating types in fungi (and somatogamy)

I would have some related questions about the mating types of fungi. Does a single spore generate a mycelium possessing only one mating type? If it does not in general, do Ascomycota and ...
4
votes
2answers
176 views

Can carnivores survive on a (processed) vegetarian diet? Why or why not?

There are many differences in for example the digestive system between carnivores, herbivores and omnivores. It seems that in certain kinds of species adaptability with respect to diet is possible. ...
1
vote
0answers
31 views

Load specific sequences in with BioSeqIO [closed]

I am trying to load a multifasta file with Perl using my $in = Bio::SeqIO->new( -file => $entrance) and then I loop trhough the file looking for id i ...
3
votes
4answers
88 views

Can systemic intravascular thrombosis cause brain infarction?

I wonder if the thrombus can pass through the brain-blood barrier because I think small molecules like O2, CO2 and ethanol can pass it. Probably, in some diseases where the permeability of the barrier ...
9
votes
2answers
985 views

What kind of beetle is this?

I live in Denmark, Europe and I found this insect wandering around in my garden the other day. It's some kind of beetle but i dont know what kind it is or the species. The beetle could also fly and ...
0
votes
1answer
203 views

How does amiodarone act as an alpha and beta blocker?

Amiodarone is potassium channel blocker but also voltage-gated Na+ channel blocker according to Pubchem. However, upon reading my notes I discovered Amiodarone blocks potassium channels and block ...
1
vote
1answer
274 views

How much heat can a human sustain? [closed]

For how long can a human sustain a temperature of 180 degrees Fahrenheit (82oC) without damage in a confined place? For instance, suppose a person is sitting in a steam-filled room, without external ...
12
votes
1answer
588 views

What is the scientific name of this plant?

I found this one on a hill here in Palestine. It's about 20 cm in height. Note that it does exist in this regions, but scantily.
2
votes
1answer
407 views

Explanation of protocol in DNA extraction experiment

In the Materials and Methods section of this paper on DNA extraction and analysis from hair I do not understand some parts of their protocol methods. In particular: "The digested hair solution was ...
0
votes
1answer
69 views

X-recessive disorder pedigree confusing me

This is a pedigree of a family in which bleeder disease, an x-recessive disease, is present. I am asked which marker, A or B, the disease is linked to. I've asked about this type of problem before: ...
3
votes
1answer
187 views

How does rate of evolution/innovation scale with population size?

I understand that there will be many other factors that affect rate of evolution/innovation. However, other things being equal, how will the rate of evolution vary between two populations of different ...
4
votes
2answers
89 views

Brain regeneration - book recommendation

Searching for an up-to-date book on regenerative brain medicine with a focus on stem cell therapy. Also interesting in genetic engineering of neuronal stem cells for this purpose. Alternatively, a ...
6
votes
2answers
216 views

Can plants live forever? [duplicate]

I know that some plants die for old age like a lettuce. But there are trees like Baobabs or larger Ficus in the tropics of whom we don't know their age. And trees like spruce reaching 9950 years old, ...
5
votes
3answers
792 views

A free book/resource for learning genetics?

I took an undergrad class in genetics. I felt it was not too intensive and I do not feel prepared for grad school (if I can manage to get in.) Does anyone know of a preferably free resource for ...
1
vote
1answer
66 views

Dynamic mutation and huntington's

I read that Huntington's is a disorder caused by dynamic mutations in the DNA, which means that a triplet sequence of DNA changes from generation to generation. Say we have the sequence ATGATGATGATG. ...
3
votes
3answers
57 views

Doxorubicin's intercalant and topoisomerase inhibition in leukemia?

I am thinking the role of doxorubicin's pathways in cancers such as leukemia and lymphoma: topoisomerase inhibitor and inhibit DNA activity intercalant - intercalant DNA bases and inhibit DNA ...
2
votes
2answers
87 views

How does a cell create/gain its initial ribosomes for protein synthesis?

I did some quick google searches for how a cellular organism generates or acquires its first ribosomes, but I found nothing. For instance, do the organisms initiating replication form extra Ribosomes ...
8
votes
4answers
102 views

Are there constraints on where intron/exon boundaries occur with respect to the triplet codons of the reading frame?

I did a quick search (here and elsewhere) and couldn't find anything on this subject. If all introns in a given primary transcript were spliced out in the same way, then this wouldn't matter. But ...
2
votes
1answer
40 views

Workspace preparation and cleanup for DNA work [duplicate]

What steps should be taken in a molecular lab environment to help ensure that DNA samples/stocks are not contaminated, or contaminate other objects in the lab?

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