8
votes
1answer
624 views

How to prevent protein precipitation?

I'm trying to label a protein with fluorescent dye (TMR succinimidyl ester), but having trouble getting the protocol to work. The protein seems to be stable in distilled water at a the recommended ...
4
votes
1answer
181 views

Cloning two fluorescent proteins with different promoters in the same plasmid?

I want to clone two fluorescent proteins both driven by different promoters in a plasmid to be used to transform B. subtilis at the amyE locus. For this I want to use plasmid pSG1729 (Lewis and ...
0
votes
1answer
62 views

Function of carapace

Majority of crustacean larvae have expanded carapace drawn out into processes as an adaptation for : a. Protection from enemies b. Planktonic habit The answer given is b but I think it should be ...
3
votes
1answer
55 views

Euthyrodism and goitre

I read in Tortora and Derrickson that goitre is associated with euthyrodism. How is that possible?
0
votes
2answers
146 views

Can you detect if a mutation is spontaneous or induced?

Is it possible to determine if a certain specific mutation had a spontaneous origin (for example from a mistake of the DNA polymerase) as opposed to an induced origin (for example, from some genotoxic ...
1
vote
1answer
24 views

Giving diuretics to insipidus patient

I read in Tortora and Derrickson that giving diuretics to diabetes insipidus patients may in fact alleviate the symptoms of the disease. How is this possible?
6
votes
2answers
840 views

Does body temperature affect human lifespan?

If a human body were somehow to be kept at a cold temperature (say 30C) for the entire life of the given individual. And somehow the brain could be fooled into thinking that this 30C feels nice and ...
2
votes
1answer
249 views

Population Growth Rate Question?

A population of 600 birds faced a problem of biological magnification resulting in a large number of deaths reducing their population to 350. But 230 births also took place and 21 birds immigrated ...
1
vote
2answers
218 views

Question about production of CFTR protein

How do organelles such as ribosomes, ER, Golgi and vesicles produce CFTR protein and mucus?
1
vote
1answer
157 views

Effect of pH on life? Use buffer or HCl? How about lactic acid?

I'm trying to find the effect of pH on living things like E. coli and such. At first I thought of using a buffer solution which maintains its level of pH, but it's possible that the inactive ions of ...
3
votes
3answers
75 views

Can genes that activate transcription factors also called be called transcription factors?

If the sole known function of a gene is to activate a transcription factor, would that gene also be considered a transcription factor, or is there a word for such genes that are further upstream on ...
1
vote
1answer
92 views

Detecting UV by humans

From my experience humans can detect UV - not by seeing it but by pain in their eyes. Many people (me too) feel pain when looking at UV lamps. When looking at sun through clouds I still can feel pain ...
1
vote
1answer
78 views

Do mitochondrial DNA have repair enzymes?

Mitochondria are considered endosymbionts originated from a prokaryote. So, do they have a DNA repair mechanism ?
4
votes
1answer
93 views

Which of Perutz et al's protein structures other than 3₁₀ helix were correct?

In 1950, Bragg, Kendrew and Perutz published "Polypeptide chain configurations in crystalline proteins" (open access) and were famously 'proved wrong' by Pauling, Corey and Branson the following year, ...
1
vote
1answer
867 views

Is Zymase, A Complex of Enzymes? Which ones?

Some websites state that the enzyme zymase (which I understand to be a complex of several enzymes) is responsible for catalyzing glycolysis in order to produce pyruvate. On the other hand, some ...
4
votes
1answer
144 views

Function of papain in papaya

I know that papain is a proteolytic enzyme present in papaya. It is often used to tenderise meat. What is the function of papain in papaya? Is papain sweet? Does it function as an antibiotic?
6
votes
3answers
14k views

Differences Between Protein Motifs and Protein Domains?

I am in a 300-level molecular biology class and am unclear about this concept and how to delineate motifs versus domains of proteins. Any suggestions would be much obliged.
0
votes
1answer
263 views

Why are human eye colours limited?

Related to the question What causes the colors we see in eyes?, I am wondering why human eyes are restricted in colour. Most common eye colours are blue, green and brown. Why not yellow, orange, ...
5
votes
0answers
306 views

Reason for seeing grid-like patterns when strobing a solid black-white image?

I was trying to do some stimulation for SSVEPs by strobing a solid black square and a solid white square. Weird thing was, I ended up seeing weird grid like patterns that looked like this, always of ...
3
votes
1answer
215 views

Ventral stream pathway and architecture proposed by Poggio's group

Please can you give me a very brief explanation about all functions in the ventral stream architecture summarized in this figure: This figure is from Serre et al.'s A quantitative theory of ...
2
votes
2answers
129 views

Sources for common laboratory Saccharomyces strains?

I'm used to working with bacteria - some of the more common laboratory strains sometimes come with an order from some vendors. What's the most convenient source to order common straings of ...
9
votes
2answers
155 views

Why is selfishness the 'obvious' strategy?

Thinking of behaviours an organism can adapt, at the very base an action can always be either selfish or altruistic (cooperative). Usually, selfish behaviour is assumed to be the preferred choice and ...
2
votes
3answers
144 views

Why are things conscious?

I've been referred to here from http://philosophy.stackexchange.com/questions/8052/why-are-things-conscious. Could you guys help out? Here's the question: What is the reason for animals or more ...
6
votes
2answers
307 views

Why are humans and almost every species on earth symmetrical?

I admit I'm not very learned on the subject of evolution, I just started researching it for myself about a week ago. One of the things that I don't understand is why humans and almost every other ...
3
votes
2answers
143 views

Why only heterogeneous SNVs for validation using genotyping arrays?

I am trying to validate the variants I found using whole genome sequencing . The standard practice, I have seen in the two publications below were to check for the number of heterozygous SNPs called ...
24
votes
4answers
12k views

Is there any advantage to one blood type over another?

All humans can be grouped into ABO and Rh+/- blood groups (at a minimum). Is there any advantage at all to one group or the other? This article hints that there are some pathogens that display a ...
0
votes
1answer
44 views

Reassociation of DNA

What is the order in which this DNA samples would would reassociate ? e.coli DNA plasmid DNA viral DNA satellite DNA DNA-RNA hybrid This is a homework question and I have not been given any ...
1
vote
2answers
31 views

What are examples of genes succesfully reproducing in ways that is in conflict with helping the individual creating offspring

Most of the time, our genes have common interests with our "self". We reproduce well if and only if our genes reproduce well. In some cases, like in bees, the haploid bees can survive better if they ...
0
votes
1answer
183 views

What is meant by 73 hour birth [closed]

I was reading an article, when I read a sentence saying, His mother ... nearly died less than three years later during Mathers' 73-hour birth. What does mean by 73-hour birth?
4
votes
1answer
1k views

What does conditional analysis of a SNP in a GWA study entail?

I am familiar with the use of tag-SNPs in genome-wide association studies to identify gene loci involved in complex traits, but I keep seeing the term "conditional analysis" used without any ...
2
votes
0answers
76 views

A number of questions regarding chemotaxis assay using PBMCs

In our lab we would like to study the chemotaxis of PBMCs towards conditioned medium obtained following treatment of cancer cells with different compounds. My questions are regarding the method of ...
0
votes
1answer
9k views

How reversible is decerebrate posturing caused by brain stem damage?

This is a follow-up question to How likely would Abraham Lincoln be to survive his wounds today? You don't have to see a CT scan or autopsy to know if the brainstem is injured (directly or ...
2
votes
1answer
100 views

.Net DNA/Codon Parser

Does anyone know of an .NET (C#,VB.NET, maybe F#) DNA parser? If not I will probably make one, it doesn't seem like it would be hard. My general idea was to generate something that would report the ...
4
votes
2answers
74 views

Transmission of disease from mother to fetus

Can parasite, microbes be passed from mother to fetus through the placenta ? I know some like HIV can be.
1
vote
0answers
25 views

How is saltatory conduction faster than conduction of unmyelinated fibers? [duplicate]

It's always the same explanation that currents are able to "hop" along Ranvier nodes instead of passing continuously along the axon making saltatory conduction more efficient than continuous ...
3
votes
1answer
518 views

Why re-label the strand ends in 3' DNA labelling?

I have a problem with a molecular biology question; I don’t understand how DNA 3’ labelling works. I took a diagram from my lesson and tried to understand with it; this is what I understood. If I’m ...
10
votes
1answer
149 views

Are there specific conditions that allow humans to see ultraviolet wavelengths

It is fairly common knowledge that the lens in its normal state absorbs ultraviolet (UV) radiation. An interesting notion has come up from time to time in my reading that suggests there are a small ...
0
votes
1answer
40 views

Cellular components of blood cells

There are different types of blood cells in the human body i.e. red blood cells, neutrophils, basophils, eosinophils, monocytes, T-cells, B-cells. What are the cellular components of these cells? ...
5
votes
2answers
110 views

There are linear and rotary molecular motors in the cells. Do any of them have a fixed or stable frequency or speed?

Are there any linear, rotary or oscillatory molecular motors in the cells which can have fixed frequeny and which can be used as a reference for elapsed time timer? This question is with relevence to ...
1
vote
1answer
134 views

Why do I have a lot in common with my mother? [closed]

I have not spoken to my mother in 15 years but recently connected with her and her side of the family. I was primarily raised in a different sub-culture after the age of 4. As it turns out I ...
2
votes
2answers
76 views

Termination of translation

What dissociates first - the last tRNA, mRNA and release factors or the subunits of ribosomes? I tried searching this from Lehninger but couldn't get a clean answer.
1
vote
1answer
63 views

What is the strength of human cornea?

What is the difference of pressure a human eyeball cornea (cornea, lens and other elements separating vitreous body from air) can handle? Or asking differently: what the outside pressure should be to ...
2
votes
1answer
44 views

How essential is vitamin B3 to the diet?

I know this might seem contradictory since by definition a vitamin is an essential part of the diet but I have read that nicotinamide, a form of vitamin B3, can be synthesised from L-tryptophan. Is it ...
-2
votes
1answer
101 views

What are uses of fossils? [closed]

What are the geological uses of fossils and how could it be utilised? From The Institute for Creation Science: Fossils are typically found in sedimentary rock, almost all of which were originally ...
2
votes
1answer
45 views

How can a respiratory infection lead to a skin infection?

Example of a case: 5 children develop a bright red rash on the face and turns violet after a few days and then disappears. Then maculopapular rash appears on the trunk, buttocks and extremities. It ...
2
votes
1answer
58 views

Antibody production in secondary immune response

Can IgG antibodies be produced in the secondary immune response without the help of T cells? Is the affinity of antibody for antigens higher during secondary immune response?
3
votes
1answer
158 views

Number of genes required to sustain life

Are there estimates of the minimum number of genes required to sustain life? In what I mean by life here, I don't include viruses.
4
votes
1answer
66 views

What is the advantage of the way eukaryotes initiate translation?

The eukaryote and prokaryote mechanism for translation is slightly different. Is there any advantage of the eukaryote translation mechanism ? Edit : I specifically want to know why eukaryotic ...
2
votes
1answer
2k views

What negative effects can pinhole glasses have on the body?

While shopping in the local supermarket yesterday, I was surprised to find this display on one of the shelves. I also realize that I should have taken a pamphlet with me, as well as a picture of ...
14
votes
3answers
3k views

What causes the development of antibiotic-resistant strains of bacteria?

I understand bacteria have become resistant to antibiotics due to selection pressures, but how do resistant bacteria process antibiotics when exposed to it, compared to non-resistant bacteria. Also, ...

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