4
votes
2answers
1k views

How to convince suspension cells to adhere more tightly?

I'm developing a cell-based assay in 96-well plates that requires adherent cells, as they need to be washed at least twice during the protocol. I'm using in-house strains of HT1080 cells (some ...
2
votes
2answers
127 views

Image analysis of GFP-tagged protein localization bursts

I'm reading an article (full text here) that analyze the dynamics of localization of a GFP-tagged transcription factor (Crz1) over the time at the single-cell level, by taking movies in a fluorescent ...
0
votes
1answer
51 views

Genome Project Focusing on gene ADRB2?

I am not sure which project database to use UCSC or Ensembl for my asthma study about ADRB2 gene (Arg/Arg-16 genotype). I am using the original Human Genome Project database at the moment. However, I ...
31
votes
7answers
5k views

Why does evolution not make our life longer?

Why does evolution not make life longer for humans or any other species?
0
votes
1answer
38 views

How do cats shed their fur?

Dogs shed their fur twice a year, so I assume this is the same with cats. What is the mechanism whereby the change in temperature (assuming this is what causes the shedding) leads to the falling off ...
2
votes
1answer
130 views

Is infrared photosynthesis possible?

The post-apocalyptic science fiction novel Dark Universe by Daniel F. Galouye has some plants living inside bunkers that use infrared light for photosynthesis. There are speculations that ...
1
vote
1answer
2k views

Why is prothrombin time test not used for heparin

Prothrombin time test is used for the monitoring of warfarin but not heparin. Why is this used for warfarin monitoring and why is it not used for heparin monitoring?
1
vote
0answers
97 views

Why only able to close one eyelid? Genetic reason?

Eye-dominance is a critical thing with bows: right-eye-dominant is recommended to do the aiming on the right eye here otherwise inaccuracy. This made me think whether all people are even able to do ...
1
vote
2answers
6k views

Why is weight gain a symptom of Cushing's Syndrome?

Cushing's syndrome results from increased levels of cortisol in the body. Cortisol as I understand it however, promotes the breakdown of glycogen and amino-acids in the process of gluconeogenesis, to ...
0
votes
0answers
20 views

To prevent dry cough through reflex arch in chronic rhinitis?

Assume that the patient have atrophic nasal membranes after long chronic rhinitis. He is having frequent dry coughs through reflex arch induced by exercise, allergies, and ... You normally heal ...
6
votes
1answer
198 views

Whole genome amino acid composition tool?

I'm interested in a statistical tool to get bacterial codon usage at genomic level. Ideally, the tool should be flexible to analyse hundreds of bacterial genomes. I've looked in MeSH terms database ...
3
votes
3answers
561 views

How to calculate virus titre from qPCR

I harvested some lentivirus from 293T cells and want to titre the result. I infected 293T cells on a well plate with 400,000 cells per well which I infected with virus stock, and 1 in 10, 100 and 1000 ...
2
votes
1answer
66 views

If I sent a bag of rice to the bottom of the ocean and brought it back, could I eat it?

Suppose I filled an oceangoing container with rice and sunk it to the bottom of the ocean… say, a kilometer or so, then brought it back up. Would it be edible? Would it retain nutrients? Do these ...
1
vote
2answers
82 views

What do nerves have to do with feeling nervous?

I was thinking about how when people say: "Nerve cells don't regenerate" (which is, apparently, not true at least for some kinds of nerves) and "You're making me nervous" they might not refer to the ...
2
votes
1answer
231 views

What is the cause of dry cough?

I first thought this question in the case of the cause of dry throat after 3-day recovery of dry cough? but then I realised that the cause of dry cough can be a general thing. Assume the person ...
-1
votes
4answers
318 views

Does evolution take place universally?

Why does evolution (namely the evolution of primates into humans) take place both uniformly and universally on the earth? Why aren't there any creatures who have not taken the same evolutionary steps ...
2
votes
2answers
96 views

Is there a name for this principle in biological contexts?

This is mostly a question about usage. There is a probability-related idea that has been used in at least two biological contexts. The idea is that if something happened, it was probably likely to ...
3
votes
3answers
152 views

Help explain multi alleles in terms of actual genetic code

I understand that someones genetic makeup at an allele is usually denoted as (AA,Aa,aa). That means that instead of an A-T pair you get a C-G pair on none, 1 or 2 copies. Now, what exactly happens ...
2
votes
1answer
59 views

Coefficient of relationship and path of coefficient

A path of coefficient of relationship is defined as $$\rho_{AO} = \left( \frac{1}{2}\right)^n \sqrt { \frac{1+f_A}{1+f_O}}$$ This SE post discusses this definition From this, the coefficient of ...
2
votes
1answer
94 views

What are the Gateway clonase enzymes?

The Gateway cloning system utilizes what Life Technologies refers to as "Clonase enzyme mix" to catalyze the BP and LR reactions. What is in this enzyme mix? Is there a sequence for them?
2
votes
1answer
66 views

How to study the effect on tau protein isoforms on microtubule based transport?

From what I read, A-beta plaques inhibit microtubule based transport of mitochondria when tau protein is present in the cell. How would I be able to do a test to see if one isoform of tau is more ...
2
votes
1answer
40 views

Function of an VEGF inhibitor in the treatment of macular degeneration

I'm working on a short presentation of treatments of macular degeneration for a biomedical optics seminar on macular degeneration. Since I don't have any relevant medical or biological background ...
15
votes
3answers
2k views

What causes a 'stuffy' or 'runny' nose when you have a cold?

When humans get the common cold, a common symptom is a stuffy or runny nose. Is that the body's immune response or is that the virus's doing?
2
votes
1answer
27 views

Size of DNA in phage

I have read that DNA(after recombination) is packaged in bacteriophages lambda only if it's between 40000 and 53000 bp long. This constraint can be used to ensure packaging of recombinant DNA. I ...
3
votes
1answer
228 views

Dimerization of Immunoglobulin G

I would like to know the specific determinants for formation of IgG dimers. My understanding is the stem of the antibody is a homodimer of two heavy chains, covalently bonded through two disulfide ...
4
votes
1answer
1k views

How does a pine cone open?

When a pine cone is wet, it remains closed. However, when it's dry it opens again. From the perspective of physics or biomechanics, what is the mechanism that allows a pine cone to open and close as ...
2
votes
0answers
34 views

examples of chromosomal aberrations with lack of sexual chromosomes?

Are there any examples in animals where individuals survive having no copies of their sexual chromosomes?
1
vote
2answers
51 views

What are Antihistamines doing in a baby?

In his song Lullaby, Tim Minchin sings: Your nappy is dry and your tummy is full Of enough antihistamine to chill out a bull Yet still all this gringing According to Wikipedia, ...
4
votes
2answers
118 views

Are there special constants in biology that define organism's morphology?

In math, there are special numbers, like Pi (3.14159...) and e (2.71828...). In chemistry, there's numbers like avogadro's number (6.0221413e+23). For example a circle can be defined in terms of 2 * ...
1
vote
0answers
24 views

Why are embryonic germ cells considered stem cells?

In a class that I'm taking we were presented 3 types of stem cells. Adult stem cells which come from bone marrow Embryonic stem cells which come from embryos Embryonic germ cells which come from ...
8
votes
2answers
229 views

Why does the chance of having a baby with Down's syndrome increase with the age of the mother?

Down's syndrome occurs when either the egg or the sperm cell contain on extra chromosome 21. To my understanding, women are born with all the egg cells in place already, so there's no further cell ...
10
votes
2answers
244 views

Why is venom more common in fish and snakes than other vertebrates?

Reading this question, I wondered why is it that we associate vertebrate venoms so often with snakes and fish, and more rarely with lizards, amphibians, mammals, and birds (apparently never, in ...
1
vote
2answers
51 views

Why will tissue from identical twin be rejected in some cases?

From Medline Plus : Also, transplants from one identical twin to another are almost never rejected. My question : Why can a tissue from an identical twin be rejected ?
0
votes
1answer
38 views

evolution and mutation of microrganisms against medicines?

I presume microorganisms would have very short life span and they would multiply more creating millions of their kind. Is it really a threat to consider when we use anibiotics and other stuff more ...
6
votes
1answer
68 views

How well supported is the hypothesis that aging is mainly caused by the number of mutations in tissues?

I heard the hypothesis, that the (main) reason of aging is the increasing number of mutations in body tissues. The higher the number of mutations is, the older tissue is. Is this true? And how well ...
2
votes
1answer
41 views

Cosmid genomic library, or genomic cosmid library?

Which usage is better for referring to a "cosmid library of genomic DNA fragments"?
1
vote
1answer
156 views

Plastid and mitochondria

I am not biologist, so please bear with me for this basic question. Although I tried googling, I am confused. What is difference between plastid, chloroplast and mitochondria? Are there any plant ...
3
votes
1answer
95 views

Activation Of Embryonic Genome

Embryonic gene activation is a process by which the embryo begins to transcribe its newly formed genome.As the embryonic gene activation occurs during early stages the paternal genome may not have any ...
1
vote
1answer
34 views

About a mechanical aid to the heart

I saw a news story a few years ago (I think) about a girl with a poor heart having a device implant that took over only some of the functioning of her heart ( I think they called it a piggy-back ...
1
vote
2answers
336 views

Blast a sequence against multiple databases

I would like to BLASTP a list of protein sequences in fasta format against multiple protein databases. Since I'm only interested in the first hit of each database, it is possible that BLAST result ...
1
vote
1answer
42 views

How do I tell whether a mass spectrum was made with ESI or MALDI?

I was working through a previous exam and came across the following question (with reference to the picture shown below): "What MS ionization method was employed (MALDI or ESI)?" I cannot seem ...
3
votes
2answers
141 views

Do RNA molecules bind each other?

I have a question, maybe a naive one. Let's assume that we isolated some RNA from a tissue. Do RNA molecules can bind each other if they have the complementary sequence? I know that some small RNA ...
14
votes
2answers
2k views

What causes REM atonia to be lost in sleepwalking?

I understand that in normal REM sleep the voluntary muscles are in effect paralysed in order to prevent an individual acting out their dreams. This paper indicates that there are likley to be ...
1
vote
0answers
21 views

Image formation

I have learnt that the brain provides a perception of image due to previous memory and experience. Then how does a blind person attain vision after cornea replacement?
1
vote
2answers
109 views

Modern alternatives of DNA footprinting

Since DNA footprinting is an old method I thought there may be some more recent and effective methods to identify a DNA sequence, do you know any of them?
5
votes
2answers
156 views

Viruses: Adaptation to a new host through repeated host jumps

A friend told me, during a 3 minute discussion, that viruses that are endemic in host $A$ and make repeated jumps to host $B$ but can't be transmitted between individuals of species $B$, may slowly ...
7
votes
2answers
245 views

Can oncogenesis happen without apoptosis pathway being affected?

Has there ever been incidences of one or more cancerous cells having all it's cell-death pathways and apoptosis mechanisms intact and functional?
6
votes
1answer
1k views

Why do Bee stings cause swelling?

Why is it that when a bee stings, a little area around it gets swelled? I think it is some sort of defense mechanism of the body. But how does it help?
0
votes
2answers
86 views

how does DNA and RNA work [closed]

How do the DNA modules tell the cell what to do?
1
vote
1answer
35 views

Standard curve for real time

When performing a standard curve for some new primers to test for fold change, is it necessary to run the standard curve? I mean, in case your experiment has several genotypes for the same ecotype ...

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