0
votes
1answer
44 views

Complexity in creating transgenic animals (e.g., mice)

Many papers I have seen describing transgenic rodent models (and presumably applicable to other model organisms) involve the knock-in, or modification to, a single gene, possibly two genes. With ...
3
votes
2answers
82 views

Is there a way to measure the amount of bytes that are possible to encode in a DNA molecule?

When I saw a DNA molecule for the first time, it kinda reminded me of a hard drive. It consists of slots and there are some possible combinations for each slot; in the hard drive these possible ...
0
votes
1answer
97 views

RACE pcr product

I'm a molecular bio novice, and I'm trying to understand the differences between regular PCR and RACE PCR. If it wanted to sequence my PCR product that might be hundreds, if not thousands of bps ...
2
votes
1answer
115 views

What's the difference between a free chromosome fragment and an extrachromosomal array?

This is reference to a review on C. elegans mosaic analysis by Yochem and Herman, in which the authors make a distinction between free chromosome fragments and extrachromosomal arrays. For the ...
4
votes
0answers
55 views

Does preservation in ethanol alter leaf litter mass?

I have benthic samples that were collected with an Ekman dredge from some small ponds. The samples contain quite a bit of coarse particulate organic matter (CPOM, basically dead leaves). I would ...
8
votes
1answer
377 views

Can Bioluminescence drive photosynthesis?

I'm trying to construct an anaerobic kitchen-waste digester at home. The major output from the digester is methane - with a significant component of carbon dioxide. To scrub/reduce the CO2 I was ...
4
votes
2answers
3k views

Why is that all the animals can swim in water without learning to swim but humans cannot?

I had a question since I was a child. I was always curious that all the animals can swim in water. They don't need any training or to learn swimming. But humans need to learn to swim. Why ? I am a ...
-3
votes
2answers
104 views

Specific enzyme for C. elegans [closed]

We are looking for an enzyme that appears in C. elegans but isn't common for: human, Drosophila or mouse. We need it for a little research project, thanks for your help!
2
votes
1answer
61 views

Are (baseline) high blood levels of melatonin associated with either circadian rhythm or mood disorders?

(Exogenous) melatonin is administered at night for treatment of circadian rhythm disorders, but melatonin taken during the day is linked with depression. I would like to know if high blood levels of ...
3
votes
0answers
78 views

How does LCR compare to Assembly PCR

The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
7
votes
3answers
7k views

Why do I smell flowers after sneezing?

Often when I sneeze, I smell flowers for a few seconds afterwards. I've done some searching on the internet, and apparently it's something that is at least not entirely uncommon, although the exact ...
1
vote
2answers
83 views

Do individual (free) nucleotides base-pair with other free nucleotides?

I had a student ask me about this and my google-fu let me down. He asked if individual nucleotides (not in a nucleic acid) base-pair with their complementary nucleotides, essentially forming many ...
9
votes
1answer
115 views

E. coli values for [enzymes], [metabolites] and kinetic rates

In my attempt to create a metabolic model of E. coli, I have found a comprehensive list of metabolic reactions and their stoichiometry. The one I am currently using is E. coli model iJO1366 (more ...
12
votes
1answer
358 views

Looking for a cancer drug target database to guide sequencing of patient tumor DNA

I have a question I would like to pose to the community. I have recently received access to a bench-top ion torrent DNA sequencer. Our idea is to use this machine to sequence the DNA from patient’s ...
9
votes
3answers
1k views

Species with reproduction barriers that can both reproduce with a third species

To start with, I do not have a sound knowledge in biology or any formal education in the area. I was told that one of the definition of a species is a reproductive barrier, which means that if two ...
1
vote
1answer
486 views

Relation between goose bumps and temperature

As we all know, cold weather will cause us to have goose bumps. I honestly don't know why (1), because our skin contracts? Apart from that something quite contradicting happened this morning. I was ...
5
votes
1answer
374 views

What is the biological mechanism underlying caffeine intolerance? (CYP1A2 or other?)

As far as I can tell, caffeine metabolism occurs primarily via the CYP1A2 enzyme. I am curious as to whether mutations in the CYP1A2 gene are associated with caffeine intolerance. Some site that is ...
1
vote
0answers
23 views

What is the efficacy of Pertussis booster vaccine among different age groups?

The Murray Microbiology book says that it is prefentially 10 years, similarly Estonian and Finnish health associations. However, my professor says that it can be 5-7 years. I started to think if the ...
8
votes
2answers
598 views

Do hormone drugs affect whether a person feels sexual attraction to males or females?

I know that ingesting testosterone and other hormonal drugs may stimulate libido and increase sexual desire. But I wonder, if a man ingests female hormones such as estrogen, will he experience sexual ...
1
vote
1answer
99 views

Agony, Hydrophobia and viruses in the light of evolutionary principles

I'm undergraduate physics student, but I've always been interested in biology. So I have a couple of questions about an application of the evolutionary principles to practice. Agony as the last ...
2
votes
0answers
39 views

Vitamin and mineral contents of common fruits

I'm looking for nutritional data, mostly vitamin and mineral contents of common fruits, such as apples, bananas, oranges, kiwis etc. Are there scientific (published and peer-reviewed) sources that ...
3
votes
2answers
121 views

High sequence similarity but start codon isn't methionine

I have noticed in a particular genome sequence of a prokaryote that various regions in a sequence share similarity which is high(>80%) with known proteins. However, the start is not a methionine. Is ...
10
votes
1answer
152 views

Does sleep in humans re-structure based on the expected sleep time?

I've read about anecdotal evidence that human brain has a fairly good internal clock that can be used to judge the amount of time that has passed. I'm wandering if there are any mechanisms in the ...
1
vote
1answer
151 views

Are there any viruses or bacteria which have evolved to withstand higher temperatures due to fever

My question was raised after receiving this information: The primary reason the body raises its temperature (via the Hypothalamus in this case) is that bacteria and viruses tend to optimally ...
2
votes
1answer
61 views

Indestructible Super Shrimp

How are shrimp and other animals able to not be crushed by the 1000x greater pressure at the bottom on the Mariana trench? Wiki says the trench is home to "large living creatures such as a sole or ...
1
vote
2answers
617 views

How do animals decide what to eat and what not?

I want to know this in particular about animals that no longer eat what they used to eat. The best example in my opinion are chickens. Chickens are often held to eat all kinds of food we would ...
1
vote
0answers
97 views

Why aren't antheridia and archegonia touching?

Antheridia and archegonia are the two male and female gametangia, respectively, and they are found in bryophytes. To cause fertilization, usually a thin film of water must be present for the sperm ...
1
vote
0answers
34 views

If a receptor is inhibited throughout embryogenesis, could there be observable phenotypic differences in the adult?

So I read a journal article entitled "Maternal hypoxia and caffeine exposure depress fetal CV function during primary organogenesis" (Momoi, et al., 2012) and in essence the article speaks of the ...
5
votes
1answer
495 views

If fever is a natural immune defense, why do people take drugs to lower it?

If fever is a natural defense against pathogens, why do sick people take NSAIDs to reduce the fever?
-3
votes
1answer
116 views

How much can be said about behaviour (future) based on bloodgroup?

My bloodgroup for example is A+. How much information is knowing my bloodgroup? How much information can be known from a blood sample? I expect you to be able to clone if known a complete DNA sequence ...
3
votes
0answers
59 views

Sequencing rtPCR product

So I have a validated set of primers for rtPCR from Biorad that contains SYBR green. If I do rtPCR, can I use the rtPCR product after purifying it with a Qiagen PCR purification kit? Also, I'm under ...
2
votes
0answers
60 views

Might a previously administered MPSV4 vaccine react with a newly administered MCV-4 vaccine?

This is a hypothetical situation. Meningococcal polysaccharide vaccine (MPSV4) is a vaccine against Neisseria meningitidis. MCV4 is a conjugate vaccine. I would like to know if the polysaccharide ...
5
votes
1answer
97 views

Can cirrhosis be caused by physical compression of the body?

On a random forum, a member speculated that their cirrhosis was caused by wearing tight-fitting clothing such as a girdle or corset. This leads me to the following question: Question: Can ...
6
votes
1answer
231 views

How selfish is Dictyostelium slug formation?

Dictyostelium is a slime mold which is well known for having a single cell free living phase and other some conditions (e.g. when food is scarce) for forming communal piles of cells called 'slugs' ...
7
votes
2answers
222 views

Does becoming martyr have an evolutionary advantage?

This is related to How does "be altruist to those who are similar to you" evolve? Altruism that is Not reciprocal Not familiar has little explanation. One possible explanation is that ...
6
votes
3answers
663 views

How exactly are game theoretical evolutionary models described during implementation for computer simulations?

When a biologist or a layman tries to reason the evolutionary explanation for something, they would simply use English with some math thrown in (for a random example, pick any explanation out of "The ...
1
vote
1answer
58 views

Is there an animal which, when eaten by other animal, poisons the predator and gets out of it's stomach?

It was a question in one competition, but it remained unanswered and i am curious about an answer. There was a hint: American bullfrog but i can not find any example of this behaviour on the web.
1
vote
0answers
84 views

Cereal Alpha and Beta Amylase temperature range and reaction rate

This question is motivated by a practical application in bread making. While it is well known which are the optimal (highest reaction rate) temperatures for both alpha and beta amylase, I am not able ...
1
vote
1answer
139 views

What part of the cactus photosynthesizes?

In general, plants photosynthesize through their leaves and, to a much lesser extent, through their stems. Cacti, however, have no visible leaves, but instead spines. In what part of the cactus does ...
2
votes
1answer
2k views

Speciation by polyploidy

Speciation can occur by polyploidy. My understanding of the process is as follows: 'polyploidy is when the number of chromosomes in an organism's cell doubles. This means that the organism has more ...
1
vote
2answers
563 views

Batch convert miRNA names to Accession IDs?

Does anyone know of tools to convert lists of miR names to their miRBase Accession IDs? I know they exist, but my search keeps pulling up gene ID converters. EDIT: both @rg255 and @shigeta have ...
0
votes
0answers
139 views

Growth kinetics of E.coli on media containing lactose and casein

I did this experiment last month using E.coli isolated from raw milk and have following data via uv-spec: ...
5
votes
1answer
201 views

Are descriptive statistics sometimes more useful than tests of significance?

How does the biology community currently feel with regards to publishing descriptive and effect size statistics rather than significance stats? Almost every journal article I read in the cell biology ...
2
votes
0answers
69 views

What determines the (perceived) frequency of tinnitus?

I noticed that every time I suffer temporary tinnitus after going to a very loud concert, the frequency I perceive is identical. I'd put it somewhere around 17KHz, but it's difficult to pin down with ...
3
votes
1answer
80 views

Regeneration capabilities in humans?

To what extent can humans, (mammals in general), theoretically regenerate, and is their a way to speed up and/or exceed this original extent.
1
vote
1answer
61 views

What Conditions must Drugs satisfy for them to be Deliverable via the Transdermal route?

What Conditions must Drugs satisfy for them to be Deliverable via the Transdermal route? I assume a high $\log{P}$ (lipophilicity) would be one, are there any others?
8
votes
1answer
200 views

Are animals that live in areas where avalanches happen equipped to deal with that?

Humans take courses to learn how to assess the risk of avalanches and how to deal with it. Additionally, they often use special gear to survive avalanches if they got caught in the middle of one. ...
0
votes
2answers
70 views

What is a “tool strain”?

When a biologist is talking about a genetically engineered mouse strain which is a "tool strain", what does that mean? What is the exact definition of a tool strain? What is the difference between a ...
0
votes
1answer
1k views

How many nucleotide pairs code one gene?

As I know three nucleotide pairs (triplus) code one gene. But I have found next information in the "Molecular biology of the cell" by Bruce Alberts: Mycoplasma genitalium: it has only about 480 ...
1
vote
1answer
1k views

What is the appropriate blender speed to maximize nutrition and digestion of fruits and vegetables?

Blender companies state that a blender for smoothies should have speeds of at least 30000 rpm. They argue that only at 30000 rpm are cells of fruits and vegetables sheared enough to maximize nutrition ...

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