4
votes
1answer
208 views

Cause of short sightedness/ far sightedness

I recently had gone for an eye test. The doctor told that short sightedness/ far sightedness is caused due to shrinking or expanding of the eye. One thing i forgot o ask him was what causes that ...
3
votes
1answer
98 views

Cucumber on the eyes

In many spas and salons I have seen people place a cold cucumber on their eyes. I think this might be for coolness, but why a cucumber? And Is it actually for coolness or for something else (like ...
19
votes
1answer
10k views

How does laughing gas (N2O) work?

Laughing gas (N2O), well, makes people laugh. How does just a gas make us do that, there has to be some hormones at work... So, I wanted to know how this works? What is the mechanism?
1
vote
0answers
54 views

Are the intestinal flora substantially different within a diverticulum of the colon?

From the Wikipedia page for the vermiform appendix: This proposal is based on a new understanding of how the immune system supports the growth of beneficial intestinal bacteria, in combination ...
8
votes
1answer
945 views

Is there a maximum amount of antibodies your body can keep?

I am wondering if you were theoretically able to get vaccinations or antibodies for any and every diseases and/or illness, would there be a limit to how many you can get and keep in your body at one ...
6
votes
1answer
102 views

Bicoid regulation of hunchback

I'm learning about development via the example of Drosophila embryogenesis. I understand that bicoid regulates hunchback, among other genes. My question whether the regulation is direct or indirect? ...
7
votes
1answer
354 views

How Does Green Tea Increase Metabolism?

According to the article "Green Tea Supplementation Affects Body Weight, Lipids, and Lipid Peroxidation in Obese Subjects with Metabolic Syndrome", Green tea increases metabolism and fat loss: ...
2
votes
1answer
5k views

What are the differences between white and brown adipose tissue?

What are the differences between white and brown adipose tissue? Here are some that I have come across: ...
4
votes
1answer
177 views

intravenous (IV) in the tail vein of an anaesthetized mouse

I trained myself to do intravenous injection on live mice. However, on anaesthetized mice you don't see the flux of your product showing that your injection was successful. In the same way, on live ...
9
votes
2answers
299 views

How is human biological clock modelled in modern science?

Im an engineer by education and i program a lot, so the question sounds weird to people from other disciplines. Im trying to better understand the human biological clock. Yet i do not really know ...
4
votes
2answers
681 views

Why is proteinase K digestion performed at 50 °C?

Many DNA isolation protocols use a Proteinase K digestion to remove proteins. This is often performed at 50 °C. Why is this?
7
votes
3answers
484 views

The evolution of dogs through domestication and artificial selection

I am currently reading Richard Dawkins's book 'The Greatest Show On Earth: The proof for evolution' and in the second chapter he talks very much about the evolution of dogs. He says centuries ago ...
13
votes
1answer
2k views

Why do we grow so much hair on our heads compared to our bodies?

I've been wondering about head hair, facial hair in particular. Human males can grow very extensive beards should they choose to not shave - however you do not really see this in our chimpanzee ...
4
votes
1answer
51 views

How to find ion/water channel related genes

We now have a collection of transcripts at hand. We would like to investigate some particular ones, which are ion/water channel related. How to perform this? Could anybody point out how to find the ...
3
votes
2answers
108 views

What risk to DNA does long-term exposure to low-dose radiation pose?

A new study from MIT scientists suggests that long-term exposure to low-radiation poses no risk of DNA damage for mice (it is also important to note that mice are unusually susceptible to cancer). So ...
6
votes
2answers
361 views

Modern Whales with Vestigial legs Myth?

Is it a myth that modern whales have been found with hind legs sticking out of their sides and full formed tibias, fibias, and toe bones? I keep finding assertions, but no citations. For example, the ...
1
vote
1answer
940 views

What are the positive and negative effects of insulin on cognitive function?

A UCLA study seems to imply that insulin interferes with cognitive function. The DHA-deprived rats also developed signs of resistance to insulin, a hormone that controls blood sugar and ...
4
votes
1answer
55 views

What value type would a chromosome position be in a database or form?

I wanted to create a tool for some fields like SIFT, Phenotype, etc..so for example I know Phenotype will have "Text" values or SIFT will have some determined values from a drop down list...but what ...
5
votes
1answer
169 views

Are quaternary protein monomers unique to a particular protein complex?

I know that quaternary protein structures are formed exclusively via non-covalent bonds. My biochemistry professor discussed a viral capsid that is essentially one quaternary structure with 240 ...
1
vote
1answer
179 views

Human Body “Fat” Equilibrium

Here's my theory: If you consume x calories each day with y amount of caloric expenditure each day you gain zero weight, regardless of x and y being out of balance. If you then mechanically ...
6
votes
1answer
72 views

Question: Annotation search in scientific literature

In a scientific paper I have been studying ("Predicting Novel Human Gene Ontology Annotations Using Semantic Analys" S.Draghici et al., 2010), I have read that authors checked the accuracy of their ...
10
votes
2answers
169 views

What makes E. coli yellowish?

LB, TB media, and yeast extract are also yellow but at the heart of the question. What chemical(s) make E. coli yellow?
6
votes
1answer
184 views

Decreasing the alcohol proof and faster in hangover, why?

My russian friend says that the USSR agents used/use the trick that they offered the victim first vodka and then last wine. I have noted that this puts you faster to hangover: decreasing the alcohol ...
4
votes
2answers
80 views

Genetic networks vs genetic architectures?

What is the difference between the terms genetic network and genetic architecture? I've heard both in a variety of contexts used by different people, so I am interested in what people think they mean, ...
1
vote
2answers
2k views

Restriction Mapping of Plasmid Assignment

pUWL22 is a circular, double stranded, 10.5 kb plasmid. It contains a gene encoding an enzyme that confers ampicillin resistance in the host bacterium. Cloning into the kpn I and Sst I sites ...
10
votes
1answer
12k views

How to store vegetables in the refrigerator: In plastic bags or not?

My wife and I are having a debate similar to this one: I claim that it's better to take the fresh veggies out of the bags and put them in the crisper with humidity control because: That's what the ...
32
votes
4answers
14k views

Death because of distilled water consumption

One of my friends said that I would die if I drank distilled water (we were using it in a chemistry experiment) I gave it a go and surprisingly did not die. I did a bit of Googling and found this ...
9
votes
5answers
600 views

Finding and Comparing Homologous Genes

I have a gene of of interest that I would like to compare between homologues. How does one go about finding a gene from known coding sequences across phyla? Afterwards I imagine I could do a Clustal ...
6
votes
1answer
349 views

Is it purely the nervous system causing vaginal lubrication (arousal)?

My girlfriend was watching some documentary on TLC about a paralyzed woman getting pregnant. I believe that woman still has some feeling, as she spoke about feeling the effects of a bladder ...
10
votes
1answer
2k views

How does translational coupling work in prokaryotes?

Today I heard about a phenomenon called "translational coupling", where the translation of one protein influences the translation of another protein. The messenger RNA levels don't seem influenced. ...
11
votes
3answers
302 views

Number of beneficial mutations cataloged?

I can see from Wikipedia that there are possibly thousands of harmful mutations that have been cataloged and linked to disease. There are also unnumbered neutral mutations. But, does anyone know how ...
5
votes
1answer
556 views

What generates variation in a species?

What is the biological mechanism behind the variation within sexually reproducing species? Obviously, the children are combinations, to differing degrees, of their parents. But how does the variation ...
5
votes
2answers
599 views

How long is DNA stable in a freezer?

Inspired by the post about extracting pet DNA, how long would genomic DNA be stable for in a -20°C freezer? It is common practice to store DNA (double-stranded, plasmid) in a -20°C freezer in ...
8
votes
2answers
126 views

What is the effective relatedness of inbreeding?

If a human inbreeds with a relative, how distant does the relative have to be before the homozygosity in the child is no higher than if the mate were randomly chosen from the global population?
10
votes
1answer
2k views

How to DIY preserve pet DNA today so that it can be used in 20 years

I know that there are companies that offer a kit+storage of your pet DNA for around $2k. My question is if there is any other option for doing this yourself with a thought in mind that in 20+ years ...
9
votes
2answers
137 views

Is there any difference in terms of personal healthcare between complete DNA sequencing and SNPs genotyping?

There are several companies (most notably 23andMe) providing SNP genotyping for individuals claiming that there is no sense for individual in obtaining full genome sequence since only small part of it ...
1
vote
0answers
51 views

SAM and Reverse Complements [closed]

Assume a read aligner (such as e.g. BWA) is fed with a pattern P = GACT. Now, the text that ...
18
votes
2answers
2k views

Do gene expression levels necessarily correspond to levels of protein activation?

I have seen a lot of research into molecular mechanisms of diseases/phenotypes use measures of RNA as a 'proxy' for the level of protein available in the cell. Is this actually valid? My problem ...
11
votes
2answers
77 views

determining genome-wide exogenous binding of pathogens to host genome?

I've read this paper where they specifically modify a region in the rice genome to ablate the binding site of a pathogen, Xanthomonas oryzae, and disrupt the hijacking of a gene network in the rice ...
7
votes
1answer
174 views

Protein construct design

I am trying to create some constructs of a certain protein deleting well defined domains (at either terminus) to determine interaction regions with other proteins etc., 3 constructs with varying ...
6
votes
1answer
285 views

How similar are Circulating Tumor Cells and Cancer Stem Cells?

Circulating Tumor Cells (CTCs) are linked with metastasis and their presence can be used to indicate the onset of metastatic cancer. Likewise, the Cancer Stem Cell (CSC) hypothesis suggests that ...
28
votes
1answer
2k views

Is the “Great Pacific Garbage Patch” beneficial for marine wildlife?

A study was just released by researchers at Scripps Institute of Oceanography revealing a drastic increase in the amount of plastic adrift in the Pacific Ocean. According to the paper, the amount of ...
6
votes
1answer
79 views

If a non-mammal is starved of oxygen for some time, how long would it take the animal's neurons to die?

This is a follow-up question to Why do neurons die so quickly (relative to other cells) when deprived of oxygen?. I'm particularly interested in the case of reptiles, octopuses, and other ...
7
votes
1answer
72 views

How do nuclear receptors locate each other to form a DNA loop?

Nuclear receptors can influence transcription far up- or downstream from their own binding sites by looping DNA (Rubina et al.; J Mol Bio 2004). I am not sure how exactly the receptors first attach ...
6
votes
1answer
3k views

Molecularly, why can you straighten or perm hair?

I'm aware that hair can be curly because of the disulfide bond interactions in between cysteine amino acids in alpha-keratin filaments. However, I'm curious as to the biochemistry involved in ...
7
votes
1answer
93 views

obesity risk and single gene polymorphisms

I read a fairly recent meta-analysis of studies into the association between adult obesity and polymorphisms of the FTO gene (Peng et al., 2011). The paper looked at 59 studies and found that "FTO may ...
4
votes
1answer
110 views

What exactly is meant by the expression “differentially expressed”?

As far as I've seen, this expression is almost always used in relation to gene expression profiling. Unfortunately, I have no background in this area. Can someone please explain this in layman terms?
2
votes
1answer
127 views

List of species recently found of economic value

Is there a website or organization cataloging lists of biological species to have been recently found of great economic value? For example, a plant that wasn't of economic relevance a while ago but ...
8
votes
1answer
3k views

Primary function of the enzyme lipoprotein lipase

I read here and here that the function of lipoprotein lipase is to facilitate fat uptake and storage in adipose tissue. Could anyone provide a slightly more expanded explanation, without going into ...
8
votes
1answer
93 views

Why doesn't yearly screening for lung cancer decrease mortality rates?

In a large trial, screening yearly for lung cancer hasn't reduced mortality rates. Why is this? Isn't cancer best treatable when caught early? Is this because lung cancer is hard to treat anyway, ...

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