5
votes
3answers
128 views

Finding a template/oligo combination for my first PCR experiment

I'm an information technology engineer. I love biology so I research biological topics and have an interest in PCR. That's why I have decided to create a PCR machine. Everything is done now and I ...
0
votes
2answers
724 views

What part of food gives the blood red color?

Roughly, what I know is, when we eat food it goes into our: Stomach > Small Intestine > Large Intestine > Rectum. So, it just moves through a digestive pipe. What I don't understand is, what part of ...
4
votes
2answers
393 views

Why are enzyme-catalysed reactions slower at lower substrate concentration?

Suppose I'm using 200 nmoles of enzyme and 2 mmoles of substrate. The enzyme should be saturated but if I use 50 mmoles of substrate, the reaction will be faster. Why? I just can't get it! Even at ...
12
votes
4answers
535 views

Why did high A+T content create problems for the Plasmodium falciparum genome project?

The main paper for the Plasmodium palciparum genome project (Gardner et al., 2002) repeatedly mentioned that the unusually high A+T content (~80%) of the genome caused problems. For example they imply ...
1
vote
1answer
212 views

How can I create a microcapillary for manipulation of single cells?

i'm working as a diy bio. I'm finding a way to create a micro glass capillary for picking up single cells. I see this video on youtube and would like to know what is the minimum I/O diameter if I pull ...
3
votes
1answer
827 views

What is the exact principle of capillary isotachophoresis?

I know it's a type of capillary electrophoresis, but I don't get how does the separation happen exactly.
3
votes
3answers
1k views

Why does the apex of the human heart usually point to the left?

In the majority of human beings, the apex of the heart (left ventricle) points towards the left side of the body. Sometimes however (approx. 1/12000 births), a person is born with a condition known ...
2
votes
1answer
67 views

Aside from cockroaches and other insects, which species (of all kinds) are 'immune' to ionizing radiation?

I have just been reading this excellent question and answer about cockroaches ability to withstand ionizing radiation. My question is an extension of that question: aside from insects, which other ...
1
vote
0answers
58 views

How to ligate DNA to an RNA molecule

Is it possible to ligate a small DNA molecule to mRNA? I was reading a protocol from Current Protocols in Molecular Biology and one of the procedures involve ligating a DNA molecule to mRNA using a ...
2
votes
1answer
1k views

fibroblast cells and fibers

I am interested in fibroblast cells in human arteries. Here are the things that I am not clear at the moment and I could not find any answer from the literature: What are the dimensions of these ...
21
votes
2answers
455 views

Smallest viable reproducing population

What is the smallest viable reproducing population, such as in a human population. By viable I mean a population which keeps genetic defects low (enough). A very strongly related question: what is ...
5
votes
2answers
144 views

Difference between biological control and introducing species for conservation?

I have a biology assignment and we have to explain various methods and strategies for conservation, two of which are: Biological control Introduced Species What is the difference between ...
0
votes
1answer
139 views

How do you remove DNA/RNA from plastics?

I want to know how to make plastic containers free of DNA & RNA? this is just for knowledge
0
votes
1answer
36 views

Distribution of nutrients throuought a drink made of plants?

In an attempts to eat my veggies for the day in one shot, I blended together a bag of salad with some water. The mixture separated by density, a thick top layer containing the bits of leaves, and a ...
1
vote
1answer
2k views

What is a pA(2) value?

I saw this article http://www.ncbi.nlm.nih.gov/pubmed/16710314 and it mentioned pA(2) values and I had no idea what they were. What are they? What do they mean? If possible it'd be just dandy if you ...
4
votes
1answer
66 views

Why don't individuals with sickle cell trait suffer from sickle cell anemia?

Ostensibly, in people with sickle cell trait, half of the hemoglobin in their body would be defective. Is it actually the case that sickle cell hemoglobin is produced in equal amounts? If not, how ...
1
vote
0answers
55 views

When a Lysosome's bonding sites all get bonded to, what is the most resource and energy efficient way to handle the used Lysosome?

Let's say a Lysosome that bonds to a hazardous material to neutralize it has all bonding sites used up. would it be more safe / efficient to excrete the entire Lysosome / hazardous material molecule ...
1
vote
1answer
183 views

Why are trichromat cone cells unable to sense ultraviolet and infrared radiation?

I understand that cone cells vary in the color they sense, is this because of wavelength, frequency, something else, or a combination of the previous? I also understand that tetrachromats can see an ...
11
votes
1answer
696 views

Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA?

I just stumbled upon an article promoting 10 mM sodium borate as an alternative to the well-known TAE and TBE buffers for agarose gel electrophoresis of DNA (Brody & Kern, 2004). They claim that ...
7
votes
1answer
291 views

What is the name of the appendage that mantis shrimp use to rub their eyes?

In this short clip, you can see the mantis shrimp using some sort of feathery arm/claw to rub its eyes. Looking at a diagram here, it appears possibly that it's using maxillipeds. Someone with ...
7
votes
1answer
146 views

Why must a planet be in a star's “habitable zone” to have life?

I have seen many articles that talk about a star's "habitable zone" as the ring around it which is just the right temperature for planets to have liquid water, which is necessary for life. My question ...
5
votes
2answers
534 views

Possibility and feasibility of producing designed antibodies with bacteria

As stated here it is possible to produce fragments of antibodies in bacteria and harvest them (from the medium, I guess, but I don't have access to the full article). As it is possible to design ...
5
votes
1answer
135 views

Descendants of dinosaurs

It is commonly accepted that birds are descendants of dinosaurs. It is nice that not all dinosaurs died out. Are there other animals, descendants of dinosaurs, that survived ? (Maybe not till our ...
0
votes
2answers
128 views

Blood circulation and blood pressure in different tissues [closed]

The volume of blood coursing the blood circulation is approximately five litres. A typical vein will stretch about eight times as much as corresponding artery. Because veins have high capacitance, ...
8
votes
2answers
6k views

Agarose vs agar? Why do DNA gels use agarose only and how do you obtain agarose from agar?

Agar is a relatively cheap substance from red algae. And it contains a saccharide agarose as well as a small amount of pectin. Agar is used for culture plates as is, but for DNA gels a grade of ...
3
votes
0answers
168 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
4
votes
1answer
186 views

Reason behind formation of different shapes of cell

I have always wondered how cells are modified to give them a specific shape. On what specific part of a cell does gene regulation act for giving the shape ?
1
vote
1answer
120 views

Single Nucleotide Polymorphisms and diseases

I am writing a report on how Single Nucleotide Polymorphisms occurring in each of these regions: Transcription factor (TF) binding sites Epigentic signals Splicing variants MicroRNA binding sites ...
1
vote
1answer
101 views

Bacterial division and mutation rate

When a bacteria A divides it produces two cells A', A''. Each of them receives a copy of the chromosome/plasmids. Now, DNA replication occurs way before division in a semiconservative manner. That is, ...
1
vote
2answers
60 views

How can pasteurized milk last 7 days?

In Estonia, the product bought from the shop lasts about 2-3 days, in Finland 3-5 days and In Oslo Norway 7 days. The better infrastructure of transportation explains some for Finland and Norway. But ...
2
votes
1answer
200 views

How do PLINK files and HapMap Phased files differ?

I know that PLINK and HapMap files show the same information, but can you give a thorough explanation of how exactly they differ.
1
vote
1answer
51 views

What are the biological and nutritional benefits of having numerous smaller meals a day?

I have heard here and there that instead of consuming 3 largish meals (the traditional 3 meals a day), that consuming several smaller meals throughout the day is beneficial for health (and weight ...
1
vote
0answers
157 views

What naturally produced drug causes 'a tingleing euphoric rush' [closed]

What naturally produced drug causes a 'tingleing euphoric rush' through your body, as well as a 'wave of goosebumps'? I've always been interested in this sensation, two of the last times i can ...
6
votes
3answers
162 views

Why are males more likely than females to have autism spectrum disorder?

The male to female ratio in autism spectrum disorder is around 4:1. However it seems ASD is not a simple X-linked disorder. Then how is it possible males are more susceptible than females, if the ...
8
votes
3answers
236 views

Is there any documented evidence for suicide among vertebrates other than humans?

Is there any documented evidence for suicide among vertebrates other than humans? Lemmings not accepted !
4
votes
1answer
1k views

Can breathing in helium (from balloons for example) cause damage to the throat in the long term?

Often an activity to do at a party, breath in helium and speak like one of the chipmunks. But, I am wondering, would infrequent inhalation of helium cause long term harm to the throat? in particular, ...
2
votes
2answers
87 views

Can humans' ability to dive with only breathing modifications tell us anything about our evolution?

Can humans' ability to dive with only breathing modifications tell us anything about our evolution? For example, it is possible to imagine a human anatomy that would be destroyed by the pressure of a ...
3
votes
1answer
129 views

Differences between right and left handed people

Are there any articles reporting any significant differences in behavior, attitudes, intelligence, appearance, etc., between right and left handed people?
5
votes
2answers
142 views

Could cancer be in itself a evolutionary process?

Could cancer be in itself a evolutionary process? Maybe in some way could it be a process of variation? Or would this idea be completely without support, if so, why? I don't mean that each case ...
1
vote
1answer
101 views

What determines whether I will have any dream or not?

Sometimes I have many consecutive "dreamfull" nights but sometimes there is no dream during week. Why? What determines whether I will have any dream or not?
2
votes
1answer
103 views

Electron transport in granal stacks

See paper for distribution of PS-I and PS-II within grana As explained in the above paper, the PS-I complex is primarily located within non-appressed thylakoid region and stroma lamella, while PS-II ...
0
votes
1answer
58 views

Can I control my dreams?

Is it possible to completely realize that I am only dreaming and to completely manage my dream? Thank you.
3
votes
1answer
143 views

What is the difference between vegetable and fruit?

Is there any exact definition of vegetable and fruit? How can new plant be classified?
1
vote
0answers
147 views

Ligase Chain Reaction: Determining the annealing temperature for gene synthesis

Using the tool Gene2Oligo I have a set of DNA oligonucleotides to synthesize a gene using ligase chain reaction (LCR). The average melting temperature is 72 degrees Celsius. I am going to use a the ...
1
vote
1answer
228 views

Mapping a mutation to known SNP, 3' UTR, miR

I've parsed out a very large TCGA cancer ssm (single mutation file) file to give me the essential information. The ssm is in the following format: ...
1
vote
1answer
91 views

Statistical Analysis of Protein Folding Problem [closed]

I’m new to the field of protein folding. I’ve been searching and came across some books for predicting structures (Introduction to Protein Structure Prediction: Methods and Algorithms). Does anyone ...
1
vote
1answer
91 views

Modeling yeast biochemical pathway using enzyme kinetics

I need enzyme concentration and metabolites concentration values to en-corporate these values in my model and do some simulation. I searched through some database yeast metabolome database ...
7
votes
1answer
2k views

Is it true that oral sex exists in non-human animals?

For example, outside of humans, do other species of animals engage in oral sex, or any oral stimulation of others' genitals purely for pleasure, not any cleansing habits, etc.? Basically, what I am ...
0
votes
2answers
73 views

What nutritious insects exist to farm that require the least attention?

What nutritious insects exist to farm that require the least attention? I'm looking to automate simply, in a self regulating way. Edit: To add detail, I imagine the insects would live densely in a ...
6
votes
1answer
2k views

Why do neurons have a negative resting potential?

Neurons expend the majority of their energy powering ion pumps to maintain the chemical gradients that power their electrical activity. To have a negative resting potential, neurons leak potassium ...

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