0
votes
1answer
85 views

Hypothetically, what is the smallest functional form of human?

Since the my first encounter with doraemon manga, I've been wondering many things, one of them is the small light. Assuming we had an instrument, such as small light, which enabled people to grow and ...
2
votes
0answers
44 views

What are these fleeting sea worms? [closed]

I filmed the creatures below this August in the Mediterranean (specifically, the port of Fornells in Minorca of the Balleares). The images were taken from a boat moored just outside the harbor and ...
2
votes
0answers
32 views

How has the use and subsequent ban of DDT affected insect populations?

Living in Australia there were less ticks for example during the use of DDT, now there are more ticks prevalent here. I am wondering if any insect species have declined permanently as the result of ...
0
votes
1answer
996 views

What is the difference between SNP and STR?

I thought that these were just different format of the same data. But it seems there isn't a way to convert SNP (single nucleotide polymorphism) data to STR (short tandem repeat) data. Am I right? ...
1
vote
1answer
88 views

How do I compare the variance of a distribution of species in one area versus another?

It's possible that this question is better suited for stack overflow, but I think the ecologists on this site might be better equipped to answer it. I'm trying to study two geographically-isolated ...
3
votes
1answer
320 views

Is there a difference between pig and cow anatomy that makes religious slaughter of the latter less painful?

This question is not for the squeamish. I was told that non-Kosher animals like pigs have a different anatomy in their neck compared to kosher animals like cows such that the Shechita method of ...
3
votes
2answers
269 views

Why is there such an argument about evolution?

DISCLAIMER: I am a Christian, although I happen to believe that some form of guided evolution via natural selection is God's mechanism for creation. Now to my question, which is related to my ...
1
vote
1answer
73 views

Identification of this plant?

Photographed in Rocky Point, Mexico. Any ideas about this plant? Thank you!
2
votes
2answers
590 views

What can cause incompatible sticky ends to be ligated?

Actual question I have reason to believe (details see below) that in a ligation I carried out, an EcoRI sticky end (EcoRI: G'AATT_C) and an XmaI sticky end (XmaI: C'CCGG_G) were somehow ligated ...
3
votes
1answer
205 views

Western blotting questions?

everyone. I've just been introduced to the procedure of Western blotting from my reading, though I'm not entirely sure about some points. I'd appreciate it if someone could help me with this. What ...
4
votes
1answer
187 views

What is the evolutionary advantage of regret?

Some motivational speakers may say that regrets are only useful to learn something from. What are the other advantages?
6
votes
0answers
103 views

Does Lucid Dreaming decrease the regenerative effect of sleep?

After watching this SciShow video on sleep, I wonder about lucid dreaming. Specifically: Does lucid dreaming and associated directed agency within Lucid Dreaming decrease the regenerative effect of ...
6
votes
3answers
231 views

If I built a concrete pool, would it ever contain fish?

Imagine that I built a concrete-walled pool the size of a football field and filled it with fresh water - obtained from another lake but free of bacteria, plantlife, eggs, etc. Would it ever, over ...
1
vote
1answer
294 views

Can two denatured DNA strands reassociate?

What matter if I increase the temperature to 95°C. So double-strand DNA denatured into two ssDNA, and then I decrease the temperature to room temperature. Are they able to reassociate after ...
9
votes
3answers
5k views

Why does human facial and head hair continue to grow?

Many people can grow extremely long head hair and facial hair. Are there evolutionary theories as to why this is the case? It seems like having long hair could be a disadvantage, and extremely long ...
1
vote
1answer
80 views

(Web)app to search metabolic/signalling pathways

I'm looking for an application where you can find pathways by selecting a chemical occurring in it. So, for example, selecting 6-phosphogluconolactone brings up the pentose phosphate pathway or any ...
0
votes
2answers
66 views

Telomeres in cloning experiments

In whole animal cloning ( eg. Dolly ) the nucleus is taken from a somatic cell. So isn't shortening of telomeres a problem ?
1
vote
1answer
74 views

DNA and gene activation

As far as the genetic content of each cell is concerned I have read to my satisfaction that all cells of a person's body except the red blood cells (with no nucleus and so no genetic message) and the ...
3
votes
0answers
59 views

The Operation of tuning in the S1 layer of ventral model

According to my previous question in ventral Stream pathway and architecture, I want now to get a brief example about how the S1 layer is constructed. In other words, how all the simple units are ...
2
votes
1answer
327 views

What species is this rabbit-like animal?

This image was included in something I was browsing through. It reminds me of my lion head rabbit, but the head shape doesn't match. Is this some kind of Siberian wild rabbit?
2
votes
1answer
363 views

Can sugar alcohols, and glycerol in particular, cause intoxication?

I'm mostly curious about glycerol, which I use as a sweetener. I've been told that it can cause intoxication, because it is an alcohol. Is this true? If so, are there any good sugar alcohols which ...
0
votes
1answer
30 views

What happens between making a DNA library and screening by nucleic acid hybridization?

I understand how a plasmid library is made and how each clone is put on the nitrocellulose membrane and nucleic acid hybridization is done. What I don't understand is how each clone is "identified" ...
1
vote
1answer
350 views

Recombination frequency, linked genes, and independent assortment

Gene R is genetically linked to gene T with a recombination frequency of 0.3. Gene T and gene X assort independently from each other. Your colleague tells you that gene T and gene X reside on the same ...
3
votes
2answers
158 views

Hardy Weinberg Equilibrium problem

In a small tribal population, the frequencies of two alleles A and a at a particular locus were 0.3 and 0.7, respectively. However, not all the individuals with genotype aa could live up to the ...
1
vote
0answers
562 views

Interpreting graph of mitosis and meiosis

Note : Consider the y axis as 1,2,3,4(not 2,3,4) and x axis as time. Roman numerals should be considered from I-X ( some are not correct here ) Which stages show mitosis? My answer : III-V Which ...
5
votes
1answer
175 views

Definitions and examples of some math-releated ecology branches

I would like to have an example of application and a brief description/definition (two or three lines) of the following fields. I would like to understand the exact differences between them. Other ...
1
vote
0answers
39 views

Transduction with P1 lysate prepared from strain that is proC::Tn10

If I do a transduction with a P1 lysate prepared from a strain that is pro*C*::Tn10 and the recipient is wild type, will all my recombinants be ProC- and Tet resistant since the Tn10 is in the proC ...
11
votes
1answer
503 views

Does electricity cause damage on a cellular level?

As I understand it the mechanism of death when a mammal is electrocuted is that the current disrupts the SAN/AVN in the heart causing it to fibrilate or arrest. That's on a macro scale, however. ...
4
votes
1answer
140 views

Why are transmembrane proteins difficult to crystallise?

I know that in vivo there are a lot fewer transmembranous proteins in general, and that they are produced at a lower rate than their free counterparts. This is mainly because transmembrane proteins ...
2
votes
1answer
78 views

How to identify active protein in a complex mixture?

I am trying to figure out how to identify which protein in a complex mixture is producing a certain effect. There is an assay for the effect, so anything (a fraction of the mixture) can be tested ...
1
vote
1answer
85 views

Why use DNA polymerase in making cDNA?

RT is capable of synthesizing a complementary dna strand ( as in HIV life cycle.) Then why is DNA pol used when cDNA (synthesizing the second strand of it ) has to be synthesized from mRNA ( For eg.to ...
0
votes
2answers
133 views

Won't natural selection miss the overall minimum of a function?

Suppose we have a population of about 1000 with an environment that does not change a lot (e.g. the atmosphere is more or less the same) and we leave this population to evolve. From what I understand ...
4
votes
1answer
187 views

Stability of helical strands of DNA?

The DNA molecules contain phosphate groups involved in $3'\rightarrow 5'$phosphodiester linkages. These groups, in the bonded state with deoxyribose, contain 1 negative charges ($\ce{3'-PO4^{-}-5'}$). ...
7
votes
2answers
11k views

At what age do babies begin to synthesize their own antibodies?

When babies are first born, they receive their antibodies from their mother (I assume because they do not yet have the capacity to synthesize their own). So my question is, at what age do babies ...
2
votes
3answers
96 views

How to define “evolution”?

The standard answer to: what is evolution? is: It is a change in allele frequency over time! I believe a complete definition should encompass the following concepts: mutations copy ...
2
votes
1answer
52 views

How do you estimate global species richness?

I know how estimates of species richness in a small area are made. But how do you estimate the total number of species on a global scale? One example of such a method can be found in Mora et al. ...
6
votes
1answer
360 views

Alternatives to CFU plating for measuring number of viable cells?

I am hoping to measure growth rates of a bacterial culture in several growth conditions. I am concerned that these growth conditions may cause cell death, which would lead to a decreased ...
1
vote
0answers
36 views

Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
-4
votes
1answer
128 views

what significance is the moon? [closed]

Why is it that planets like Mercury, Venus and Pluto do not have moons? or how significant is a planet having even one moon? How does it affect the planet's ecosystem, especially seasons and periods? ...
1
vote
2answers
384 views

Gel electrophoresis question

Leena is a molecular biology student. She purifies two fragments of DNA, 800 and 300 base pairs long. These were obtained from a plasmid after digesting it with HindIII. Each of these fragments has a ...
1
vote
0answers
16 views

Cytoplasmic determinants in amphibians

If cytoplasmic determinants are distributed before fertilisation then how can an amphibian's embryo, whose dorsal side is determined after fertilisation develop ?
2
votes
1answer
90 views

What will happen if we expose the brain to an intermittent light?

If a brain is exposed to an intermittent light are specific areas going to fire? If yes, which of them? Is there any experiment about this?
4
votes
1answer
88 views

Is it true that there is a spider that eats parasites from caterpillars

I think I once saw a video of a caterpillar that could get infected by a parasite, and could die of that, unless a specific kind of spider would eat the parasite, and so once the caterpillar is ...
1
vote
2answers
104 views

Protein purification methods

Which of the following methods would yield the most purified protein fraction? A. Salt precipitation B. Charge separation C. Affinity purification The first one is the most commonly used, is it ...
1
vote
1answer
356 views

Energetics and Products of Pepsin/HCl Protein Digestion

What are the energetics of protein digestion during which the enzyme pepsin is "activated" (whatever that means) by HCl? I've looked and been unable to find anything like a chemical equation that ...
9
votes
3answers
116 views

Standard letter for 5-methylcytosine

I'm working on a piece of software that computes melting temperatures for nucleic acid duplexes, and I'm about to add support for 5-methylcytosine as a nucleotide base. At the moment, the bases ...
1
vote
1answer
56 views

How cotranscription events affect splicing?

I want to know how and how often does cootranscription takes place? Can transcription regulators influence splicing during cotranscription?
0
votes
1answer
73 views

Electrophoretic mobility of isozymes

If isozymes are seperated using electrophoresis, which of the following will be the principle of separation ? A.charge density B.molecular weight C.polarity of molecule I think it should be B.
0
votes
1answer
34 views

Ribosomes and DNA in chloroplast

Are there multiple copies of circular DNA and membrane bound ribosomes IN CHLOROPLAST ? Are polysomes found in them ?
4
votes
1answer
1k views

Basic step by step methods for PCR & Gel electrophoresis class

I'm teaching a class how to do PCR & gel electrophoresis soon and would like it if you could check through my basic step by step instructions - it's a while since I've done one. Is there anything ...

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