7
votes
1answer
6k views

What is the difference between “dikaryotic” and “heterokaryotic” states in the sexual lifecyles of fungi?

Many fungi undergo a reproductive phase in which more than one genetically distinct nuclei (from 2 separate mating types) is present within the same cytoplasm. In the Ascomycota and Basidiomycota, ...
9
votes
4answers
200 views

How can I avoid digesting protein-bound DNA?

I'm interested in sequencing and analyzing the bound DNA, and minimizing the amount of unbound DNA that gets sequenced through digestion. When digesting protein-bound DNA, is all of the unbound DNA ...
6
votes
2answers
533 views

Are cells really the basic unit of all life? [closed]

Please see comments as to the appropriateness of this question on biology SE. All known life on Earth is made up of cells. It is thus safe to say that all known life is characterized by the presence ...
5
votes
1answer
154 views
7
votes
1answer
94 views

Soma-soma paired neurons

I'm reading this paper for a presentation I have to do. Since I have no real biology background I encountered a few questions and one I couldn't find an answer using my google-fu. It's about how ...
6
votes
1answer
184 views

Effect of spaying on the female cat organism and health

Veterinarians often recommend spaying a female cat if the owner does not want her to have kittens. What does biology know about the effect of spaying on female cats' organism and health (apart from ...
8
votes
1answer
166 views

Why is maintaining a circadian rhythm important?

What are the real consequences of never maintaining a circadian rhythm? How is circadian rhythm important for health and function? Where did it arise in evolution?
6
votes
1answer
185 views

Brain + ethanol experiment suggestions needed

I need to demonstrate the effects of ethanol on a human brain (or other brain with developed telencephalon) in vitro; ideally the effects on vigilance and/or memory. Could you please suggest such an ...
9
votes
2answers
259 views

Are there differences in DNA between humans of today and humans from 2000 years ago?

Are there any significant differences in our genome compared to the genes of our ancestors from 1000-2000 years ago? And if there are significant differences, do they result in significant ...
1
vote
1answer
86 views

Does the Parasympathetic Tract of Colon Sigmoideum Travel with Nervus Vagus and its Nucleus Dorsalis Nervi Vagi?

I have the following tractus now: nucleus parasymphaticus sacrales -> nervus splanchnic -> ganglion terminalis -> colon sigmoideum The tract is parasympathetic. It suggests me that it should ...
8
votes
3answers
192 views

Spatial resolutions in optical microscopy

I have read that different optical imaging techniques such as such as wide-field microscopy, confocal microscopy or STED microscopy can theoretically achieve a different spatial resolution. ...
6
votes
1answer
201 views

Effect of histidine on the binding affinity of HisP

I was asked the following question by my teacher: A gene regulatory protein called HisP regulates the enzymes for histidine biosynthesis in the bacterium E. Coli. HisP is a protein whoes ...
12
votes
1answer
201 views

Why do people dying of immune deficiency diseases appear sick?

Please forgive the obviously silly appearance of this question, and/or of the tenor which may come across as flippant or dismissive of real world suffering. My intention is none of the above. As a ...
4
votes
1answer
89 views

Are CN3, CN7, CN9 and CN10 the only Parasymphatetic Cranial Nerves?

It has been my thought for a long time that that is the case but I am unsure about it now, since the parasympathetic tract of colon sigmoideum does not seem to have connection with CN 10. It connects ...
10
votes
1answer
129 views

Are these Gram stain substitutions acceptable?

In the context of a Gram stain on a blood smear: Are the following acceptable substitutions and/or what differences could arise by substituting them? Using methylene blue instead of crystal violet ...
9
votes
1answer
190 views

How are there alternative initiation codons?

According to wikipedia and the original complete sequence of the K-12 genome, there are multiple non-AUG start codons such as GUG and UUG. How is this possible? I'm particularly curious about the ...
5
votes
1answer
62 views

Somatosensory System

I have a test in my upcoming Neuroscience class, one of the items on the study guide is: Identify 2 means by which a maintained, constant stimulus produces only a transient excitation of the ...
4
votes
1answer
38 views

How do fibers control muscles?

My professor says Nervus troclearis IV somatomotor fibers supply musculus obliquus superior. and similarly for nervus oculomotorius III Nervus oculomotorius postganglionic fibers supply ...
4
votes
3answers
101 views

How to create a collection of anonymous sequences for teaching and testing? [closed]

I am looking for a large collection (>1000) of sequence files (eg. FASTA) from any real organism or a tool to create such a collection. The sequence files would be used for teaching and for testing ...
6
votes
2answers
563 views

For how long and how cold should I perform ethanol/isopropanol precipitations of RNA or DNA?

Precipitating nucleic acids using either isopropanol or ethanol is a very common operation, and I've read some very different protocols on the duration and temperature the precipitation should be ...
7
votes
1answer
67 views

What is the effect of exendin on beta-cells

Do you know if exendin, an analog of GLP-1 (glucagon like peptide-1), can be toxic for beta-cells? For example what is the effect on INS1 or Min6 cells at a certain concentration or after 90 mins of ...
10
votes
2answers
2k views

What is the advantage of using starter cultures for growing bacteria?

Many DNA isolation and protein expression protocols contain instructions to use a starter culture of E. coli that is then used to inoculate the main culture. What are the advantages of using starter ...
9
votes
2answers
290 views

How can I pare down a PDB file in Python to only include specific residues?

I'm trying to make a script that will take a MTZ file (reflections), convert it into a CCP4 map, then pare that map down to only encompass a desired area to cut down on the size of my PyMol session ...
6
votes
1answer
924 views

Why does the T7 RNA Polymerase require a reducing environment ie. DTT

Every bloody protocol suggests adding in DTT when doing in vitro RNA transcription. Why? The rationale seems to be that the cytoplasm traditionally has a reducing environment but as the only protein ...
9
votes
3answers
686 views

Do men have more extreme variations than women?

This question was considered unsuitable for Skeptics and I think it is more suited to BIology than Cognitive Sciences I was reading this article which I found interesting. It is not supported with ...
10
votes
1answer
526 views

Why is the 3'UTR region highly methylated in most of the human genes?

Most of the human genes are found to be highly methylated in their 3'UTR region (0.8-0.9%). I was wondering if there is any specific reason for this?
8
votes
1answer
533 views

If the xylem of a woody plant is composed of dead tissue, how does sapwood become heartwood?

If the xylem of a tree is composed entirely of dead tissue, then that means the sapwood is dead. If so, how does it transform into heartwood, and what starts the process?
3
votes
0answers
123 views

Why are trichomes important for both the roots and shoots of plants?

In what ways are trichomes vital to the plant? Why have them on the plant shoots as well?
9
votes
2answers
14k views

Why is SOC medium recommended for transformations?

In pretty much every transformation protocol I've seen SOC medium is used to grow the bacteria for a short while after the tranformation and before plating. I've usually substituted LB medium for ...
8
votes
1answer
472 views

What is a inhibitory tone when talking about neurons?

In this SE answer: Could an "overactive" brain increase the chances of Alzheimer's Disease? user @nico used the word inhibitory tone What does that ...
12
votes
2answers
401 views

Is the 'fluttering feeling' when under stress neurological or physical?

I'm sure that everyone is familiar with the sensation commonly known as "butterflies in the stomach". It is commonly experienced during periods of anxiety or stress (e.g. before high stakes job ...
3
votes
1answer
487 views

What are all possible vectors for unicellular human parasites?

I got the given question. They want the following pieces of information about protozoological human parasites: disease - parasite - vector. There are 10 proteogenic diseases at the Wikipedia. I ...
2
votes
1answer
371 views

Is Aspergillus clavatus an unicellular organism?

I could not find the piece of information form Burton Human Parasitology -book and not by Google. Yeast is divided to two phylums: one of them is Ascomycota. Aspergillus clavatus belong to the given ...
8
votes
1answer
185 views

Which organism has the sharpest vision?

Which organism has the sharpest vision in terms of distance and spectrum. Please propose any other useful measure.
6
votes
2answers
643 views

Why are goosebumps so ineffective at keeping us warm?

If the purpose of "goosebumps" is to keep us warm, why is it so ineffective? Or are the changes subtle, but important?
5
votes
1answer
224 views

Stabbing muscles when flexed and when relaxed

Just a random question, would a sharp object pierce your muscle more(stabbed with the same amount of force) when the muscle is flexed or when relaxed?
4
votes
1answer
116 views

What evidence gives clues to the physiological basis for conversion disorder?

Conversion disorder has a set of DSM diagnosis criteria, which, among other things, includes ruling out all neurological disease. However, as the media has shown us (and one could argue a biased ...
9
votes
1answer
261 views

Can Naegleria fowleri enter through wounds into the bloodstream?

All the sources I've read said that Naegleria fowleri enters the brain through the nose. But lets say that someone had cuts on their arm or leg and they are swimming in water that is conducive to ...
8
votes
1answer
796 views

Is there a correlation between muscle fibers and body types?

I have been told the number of muscle fibers a particular muscle has varies from person to person. Unfortunately, the person who told me this did not know much more than this. Initial searching on the ...
7
votes
1answer
353 views

How do fungi react to being grown in a tissue culture?

I know how plant cells and animal cells react to being cultured and grown this way, but what about mushrooms? Would they respond like plants, and result in embryos growing off the mass? Or does the ...
6
votes
1answer
113 views

On which date did the official name change of Lactobacillus sanfranciscensis change?

When did the change of official name from Lactobacillus sanfrancisco to Lactobacillus sanfranciscensis happen? An answer ((to the exact day OR within a few days) AND the name of the conference at ...
9
votes
1answer
89 views

To which distinctions does the term “hymenoptera” refer?

Hymenoptera is an order of insects that includes bees, ants, and wasps. A quick search gives the following etymological analysis of the term hymenoptera. hymen (membrane) + pteron (wing) Does ...
8
votes
1answer
216 views

How long does it take for E. coli to shift feedstocks?

With our fermentations we're noticing that it takes an appreciable amount of time for E. coli (K12 variant) to change from being metabolically streamlined on amino acids to being metabolically ...
13
votes
3answers
2k views

How does the human body metabolize gasoline?

A Chinese man has been drinking gasoline to relieve his pain for 25 years. How does the human body metabolize gasoline? Also, what are the side-affects to gasoline?
7
votes
1answer
75 views

Is there a standard format for plant community composition data?

FIA has a nice database of forest Inventory data, this would be great except that it does not seem capable of handling data from non-tree species (e.g., grasslands, wetlands). Is there a standard ...
10
votes
1answer
161 views

(How) does coppicing fundamentally alter tree growth?

I am interested in adding the ability to model coppice tree production to a model of perennial crops (Miguez et al 2008).. Implementing the biomass pools and allocation parameters required for tree ...
4
votes
0answers
73 views

What is the distance between the 3' 18s rRNA (the Kozak consensus sequence) and the A-site of eukaryotic ribosomes during protein translation?

Reposted from Quora http://www.quora.com/What-is-the-distance-between-the-3-18s-rRNA-the-Kozak-consensus-sequence-and-the-A-site-of-eukaryotic-ribosomes-during-protein-translation The Kozak sequence ...
7
votes
2answers
541 views

Did human hairs actually evolve from scales?

What is the evolution of hairs? Did they evolve from scales?
4
votes
1answer
233 views

What did pangolin scales evolve from?

Were they developed anew or a heritage from fish?
8
votes
2answers
4k views

Why should I degas my gel solution for polyacrylamide gels?

In protocols for polyacrylamide gel electrophoresis (PAGE) I often see instructions to degas the gel solution by putting it under vacuum for 10-15 minutes before polymerizing the gel. I usually ...

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