1
vote
0answers
37 views

Where can I find approximate rates of sequestration of CO2 for different species of algae?

For a study, I want to compare the rates of CO2 sequestration and fixation of a few different species of algae. I could not find any data on the sequestration rates. Any pointers to where I can find ...
4
votes
2answers
111 views

Sequencing from PCR

As far as I understand it, PCR can be used to make many copies of one gene. My question is, is it possible to sequence DNA after PCR and is it easier than sequencing it via other methods. If it is ...
1
vote
0answers
26 views

Photosynthesis/Plants Question [duplicate]

Possible Duplicate: How long will a vegetable live for after being harvested? Exactly when do plants "die," as in stop functioning to live? If, for example, you pick a plant and start to ...
4
votes
0answers
49 views

Is it possible to repopulate lactase in the stomach? [duplicate]

Possible Duplicate: Can an adult without genetic lactase persistence still develop a tolerance for dairy foods? In my younger years, I don't seem to have a problem drinking milk. Nowadays, ...
10
votes
5answers
3k views

How did the first self replicating organism come into existence?

When people try to explain evolution, they tell me that evolution is a cumulative result of mutations & natural section of the more superior individuals of a particular species. I think I'm fairly ...
1
vote
0answers
99 views

Properties and life cycle of chondrocytes and tenocytes

Questions regarding chondrocytes and tenocytes: Do these cells replicate, or are they regenerated from mature stem cells? How often are these cells regenerated? How long do they live? Do they ...
1
vote
0answers
156 views

Extreme examples of protein translation/use coupling/decoupling?

What are known extreme examples of protein translation/effect coupling/decoupling? For example, examples of proteins that are immediately used at the time the have been translated and vice versa, ...
19
votes
1answer
1k views

Why do ants live so long?

I was surprised to learn that worker ants in some species live many years. I would have expected a lifespan of a few weeks or months (which is apparently the case for many species). What factors ...
6
votes
2answers
3k views

How do I deal with sticky and viscous samples from cell lysates?

To check a protein expression I pelleted a small amount of E. coli before and after induction and lysed them by redissolving them in SDS-PAGE loading buffer and heating them to 95 °C for 1 minute. ...
3
votes
1answer
56 views

What are treatable foetal conditions during pregnancy?

What are different foetal conditions during pregnancy that could be treated with appropriate prenatal genetic testing? I only know that pregnant women are encouraged to take certain supplements to ...
5
votes
2answers
2k views

What is the appropriate method to send a strain?

We are planning to send an ecoli strain to another lab. What is the appropriate way to do so. No MTA is required but the options seem to be using filter paper or sending a glycerol stock on dry ice.
9
votes
2answers
366 views

Is there any recent evidence for the aquatic ape theory of human evolution?

aquatic ape theory suggests that many features that distinguish humans from their nearest evolutionary relatives emerged because the ancestors of humans underwent a period when they were adapting to ...
7
votes
1answer
1k views

Why is the Kakapo more attracted to humans than its own kind?

The Kakapo can be seen in this video by BBC. It is said that the species is strongly sexually attracted to humans. Why could this be the case?
2
votes
1answer
61 views

variation in expression accounted for a SNP — what's a usual percent?

I am reading a GWAS paper that found a SNP associated to predisposition to colon cancer and was assessed for gene expression of the nearby gene. They found that the genotype accounted for 55% of the ...
5
votes
1answer
120 views

Is it possible to sequence only the 16S rRNA component? If so, how?

There have recently been several papers on using 16S rRNA as a way of identifying species (here, and here). I'm wondering if it's possible to sequence either just that subunit of the ribosome or just ...
4
votes
1answer
327 views

T7 promoter leakiness

Can a gene be expressed under the T7 promoter in an E. coli strain (e.g. DH5 alpha), which does not have the T7 polymerase gene encoded in its genome? In other words, is T7 promoter leaky? To be ...
11
votes
1answer
1k views

Why does hair turn grey or white, and why does it happen later for some?

The question is pretty simple: what is happening molecularly when hair turns grey or white? I would imagine that it is due to the lack of a particular compound. I'm also interested in why some ...
2
votes
1answer
448 views

Why does ampicillin in solution turn yellow?

I have a universal tube with 10 mg mL-1 ampicillin. When I got it, it was supposed to be sterile. It was opened for approximately 20 minutes for an experiment and has since been standing around sealed ...
1
vote
0answers
318 views

Appropriate methods for triteration?

What is the appropriate method to pipetting up and down mammalians cells to sufficiently break up clumps. This should also keep in mind the issues from Pipetting damage on cells. Visually one can see ...
5
votes
1answer
120 views

Is there any reason the common housefly continues to return to an area?

This might come off as a really silly question. But I'm wondering (especially in the case of food) if there is any reason a fly would continue to try and sit on top of a piece of food even after ...
4
votes
2answers
214 views

Is Homo sapiens the only species capable of prioritization?

Just what the title states--given an absurdly large number of assignments to perform in a limited time-frame we usually attempt to do the most important ones first. Is this ability to define and ...
5
votes
1answer
328 views

Do all plants photosynthesize with equal efficiency?

Just what the title states. Given identical, necessary and favourable conditions (Probably nutrients, humidity, and light but what I mean is all necessary requirements are fulfilled) - Is ...
14
votes
2answers
773 views

Impact of Alan Turing's approach to morphogenesis

Shortly before his untimely passing, the computing pioneer Alan Turing published his most cited paper The Chemical Basis of Morphogenesis (1952). The central question for Turing was: how does a ...
11
votes
2answers
1k views

Why do only only two sexes exist for animals?

Why, from the natural selection point of view, do only two sexes exist for animals?
1
vote
0answers
32 views

Can progerin formation be triggered explicitly?

Corollary to my question - Could inhibition of progerin formation slow the rate at which a body ages? Is it possible to activate/trigger progerin formation explicitly? Say, by some hormonal/chemical ...
1
vote
0answers
61 views

Is pure (100% H20, (near to no) solutes) water toxic to living organisms? [duplicate]

Possible Duplicate: Death because of distilled water consumption I know about the effects and myths about drinking distilled and/or deionized water. I'm not asking about such substances. I ...
-4
votes
1answer
293 views

Why hasn't mother nature made us aware of our lizard brain? [closed]

In many personal improvement books I've read, people make a distinction between 2 parts of our brain: the logical brain, dealing with logic, judgement, thinking; what makes Humans different than ...
6
votes
1answer
210 views

What's the state of the art in designing and creating your own life forms?

What's the state of the art for solving the following problem. You imagine a set of features that you want a life-form to have. Just to take an arbitrary example, say you want your life-form to have ...
7
votes
1answer
147 views

Is the function of adjacent genes correlated?

Do genes that occupy a similar locus on the genome have correlated function, specifically in human beings? It is my understanding that adjacent genes are inherited together, and so location plays a ...
1
vote
2answers
429 views

Shouldn't evolution have taken care of baldness already?

It is my understanding that the hair on the head is there to protect us from sunburn. I've never seen a bald animal, so isn't evolution in charge of addressing these type of things? How is it that ...
12
votes
2answers
4k views

Do men have significant hormonal cycles?

I know there's a similar question here. But that discussion dissolved into lunar cycle and a correlation with it. I want to find more towards the original question of is there a periodic hormone ...
3
votes
2answers
175 views

Do both TSG and Proto-oncogenes have to suffer mutations to cause cancer?

I'm having a conceptual nightmare trying to understand when a group of cells may become cancerous and the more resources I consult the more confused I seem to get. In order for a cell to become ...
7
votes
1answer
69 views

How does one measure the length of telomeres?

My questions are: What techniques are available for doing so? Are they destructive ? What is their accuracy ?
4
votes
0answers
49 views

Do you know of any disease where HLA / MHC association discovery has led to better treatment or management?

If we discover an association between a disease and a particular HLA type (MHC class I or class II molecule) then it may provide us with some insight into the disease in a very basic way. However, ...
5
votes
2answers
163 views

How high is the energy yield of photovoltaics compared to photosynthesis?

I'm not an expert in plant physiology. I was wondering if, per square centimeter, leaves are converting more or less energy than photovoltaic systems. Can this be estimated? How?
6
votes
1answer
704 views

Examples of intracellular parasites of medical or economic importance?

What are examples of intracellular parasites of medical or economic importance? I have read that Xanthomonas oryzae is an intracellular parasite in rice that produces proteins able to cause changes in ...
13
votes
3answers
2k views

When has an organism evolved enough to be called a new species?

Imagine that we take a population of horses, split them in half and place them in completely different environments. The two species will evolve separate from each other and because the environment is ...
0
votes
1answer
431 views

Are there examples of the green beard effect in humans?

Green-beard effects are genes that: Produce an effect that can be detected Produce preference to others with the same effect Are there any examples of this in humans?
9
votes
3answers
1k views

Why does the butterfly have a cocoon stage in its life cycle?

Why does the butterfly have a cocoon stage in its life cycle? It could have simply grown up within the egg/cocoon, and emerged as a butterfly. Instead it is first an egg, then a worm, then a ...
2
votes
2answers
154 views

Can the body of any organism on Earth live without impairment both in vacuum, and within Earth's atmosphere?

I know from reading that a human in vacuum needs a space-suit to survive. Is the body of any organism on Earth capable of living equally in vacuum, and on/below Earth's surface within the ...
3
votes
2answers
341 views

Why Didn't Evolution Cause the Human Body to become Streamlined?

If streamlining makes movement/locomotion quicker and easier, why didn't the apes evolve into life-forms that had streamlined bodies (much like fish)?
9
votes
2answers
8k views

Hoechst or DAPI for nuclear staining?

When is it best to use Hoechst vs. DAPI for nuclear staining? They seem to be very similar on paper. Are there situations where one is clearly preferable?
3
votes
1answer
3k views

Why is beta-mercaptoethanol often added to cell culture media

Many protocols suggest that beta-mercaptoethanol is necessary for growing cells. It is a reducing agent but what does it mechanistically do for your cells. When would one not add it.
5
votes
1answer
129 views

Does every gland type have stem cells for regeneration?

Is there any gland in which there are no stem cells? I know that: sebaceous gland is maintained by unipotent stem cells thyroid gland - some stem cells, apparently unipotent Glands about which ...
3
votes
2answers
2k views

How do you calculate or predict the charge of a protein at pH 7?

How do you calculate or predict the charge of a protein at pH 7 given a fasta sequence? Any papers or online servers to do this is well appreciated.
4
votes
2answers
147 views

What is the purpose of the adrenal medulla?

The adrenal medulla is less of a 'real' endocrine organ like the others in the endocrine system and much more an extension of the sympathetic nervous system. In fact, its chromaffin cells are modified ...
2
votes
1answer
52 views

What is an efficient way to spike 10-50 cells to a culture

I'm trying to add on the order of 10-50 mammalian cells to a mixture of other cells (order of 1e6 cells). What is the best way to do this? (edit) I guess this wasn't clear. I would really like to ...
10
votes
4answers
6k views

Do we get 1/4 of our genes from each grandparent?

I know that we get half of our genes from each parent, but does it necessarily mean we get 1/4 of our genes from each grandparent? Or is it possible that we might get say 30% from one grandparent, 20% ...
3
votes
1answer
619 views

What happens when cells in your body run out of telomeres?

In my biology book I read about an experiment where the genes encoding telomerase were 'knocked out', but they could still live a normal life and no adverse effects were noticed until the 6th ...
5
votes
1answer
118 views

Is extreme cladism a mainstream position in the species debate?

In the philosophy of biology it has been claimed many times that a popular position regarding the question of what species are, among biologists, is cladism. For my current purposes, the defining ...

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