6
votes
1answer
684 views

Does anyone have any TOPO directional cloning tips? [closed]

I'm just about to start working on a TOPO cloning after I couldn't get it to work with standard restriction/ligation. Does anyone have any tips for TOPO cloning?
7
votes
1answer
44 views

Which functional annotations could be useful?

Analyzing a genome, for a generic gene, which functional annotations (e.g from Gene Ontology) can help understanding its meaning/function or, at least, provide helpful informations? Annotations of ...
4
votes
1answer
197 views

Automatic cropping of collimated digital X-ray images

Do you have any suggestions for a library which offers an algorithm to crop digital X-ray images automatically? We receive full-size images (43x43cm) from a detector, independent of the collimator ...
9
votes
2answers
34 views

Resources for finding all drugs of a certain class

I may be embarking on a project involving a fairly extensive healthcare records data set, looking for the use of a particular type of drug (for example, "Proton Pump Inhibitors"). But these drugs are ...
11
votes
3answers
496 views

Human disease and associated phenotype database?

Does anyone know of any good databases that contain symptoms for diseases and other ailments in humans? I've tried working with UMLS, but thats been impossible to work with. I've also tried working ...
7
votes
2answers
26 views

What data / tools exist for mapping of disease trends?

I was just looking at the Google Flu Trends map. Google Insight could be used to gain similar information for other trends in disease keyword searches. I started wondering how valid are keyword ...
7
votes
1answer
130 views

How exactly does long-term tension (over months) build up in the ligaments, tendons, muscles?

And is there any basis for believing that massage relieves that tension?
13
votes
2answers
144 views

Relationship between our microbiome and personalized nutrition

Recently, it has been asked whether there are 'metabolic types' between humans that can benefit from a sort of personalized nutrition. One answer suggested that one discerning factor could be the ...
5
votes
4answers
422 views

Could someone recommend an introductory book on epidemiology? [closed]

I'm hoping to learn the basics of epidemiological methods, terminology, etc. I come from a background in statistical economics, and I'm moving into the economics of public health. I don't know if ...
6
votes
1answer
2k views

Purpose of poly(A)+ RNA?

I am learning RNA-seq analysis. I always encounter this phase "poly(A)+ RNA". After searching, I got this: "Most messenger RNAs contain a poly(A) tail, while structural RNAs do not. Poly(A) selection ...
7
votes
1answer
95 views

Can one dendrite pass through another?

While tracing dendrites on EyeWire I encountered the following situation. They belong to an Orientation Selective Ganglion Cell. It's the first dendrite I've noticed with such a clear 'hole' in it, ...
4
votes
1answer
164 views

From inflammation to sickness and depression: when the immune system subjugates the brain

Recently, some research, for example this article has proposed that inflammation can cause innate immune cells to produce pro-inflammatory cytokines that act on the brain to cause sickness behaviour. ...
5
votes
2answers
2k views

Primer design for introduction of restriction sites flanking a gene of interest

I am wondering what the correct method for primer design to introduce restriction sites. Specifically between two methods. 1) Primer first partially hybridises to the gene, has a mis-match where the ...
7
votes
1answer
4k views

Difference between strand-specific and not strand-specific RNA-seq data

I would like to ask the difference between strand-specific and not strand-specific dataset. As far as I know, strand-specific data means that we know which strand the transcript is from. I do not ...
6
votes
0answers
63 views

Using the IMGT/GENE-DB service to find RSS

I'm trying to get the data for the Human and Mouse 12 and 23 Recomination Signal Sequences (RSS), to run a classification algorithm on it. I'm not a biologist, so I apologise in advance for my ...
3
votes
1answer
89 views

Majority of transcripts are from sense strand?

I am wondering whether majority of transcripts are transcribed from the 5'->3' strand? Because I encounter a lot of expressions like "anti-sense transcripts". Could anybody help clarify this? Thanks.
8
votes
1answer
1k views

Effects of beer on muscle recovery after exercise

I noticed it several times that when I drink beer (even one bottle) after some heavy workout, the next day my muscles are more stiff than other times, and not the same way... Is it because that ...
3
votes
1answer
75 views

Is it harmful for someone to consume things full of bacteria if they don't get physically sick from the bacteria at all?

Or as another example - what if you touch a surface that's contaminated with potentially pathogenic bacteria (like the ones at ...
2
votes
0answers
57 views

Are there examples of PTMs that add different chemical groups (and mass) to different amino acids?

I'm talking about this type of post-translational modifications. What I'm interested in is not whether some modifications can only occur on specific amino acids (that's clear), but if the nomenclature ...
2
votes
2answers
88 views

Consensus codon optimization by organism

Does a public database exist that contains this information? I'm trying to make a simple gene annotation program that will let me input a DNA sequence and then optimize it based on one of these tables ...
1
vote
1answer
87 views

Generating custom human DNA sequences based on traits such as eye colour?

I'm wondering if it would be possible to create software (unless some already exists, but I couldn't find any) to generate human DNA (the base pairs on the double helix) containing genes representing ...
2
votes
0answers
82 views

Mouse meta-globin mRNA problem

This is an mRNA strand of mouse meta-globin: 5'-ccccagauacggaauucgaau-3' A) Which small RNA (below) is most likely to regulate expression of meta-globin? ...
4
votes
2answers
60 views

Main methods used to predict functional annotations in GO

Can someone provide me some information about the main method used to predict the inferred electronic annotations in Gene Ontology?
7
votes
1answer
769 views

Do any animals other than humans undergo menopause?

Do any animals other than humans undergo menopause? Also, is there any difference between animals in captivity and animals in the wild as regards menopause? For example, even if menopause has been ...
5
votes
1answer
705 views

What's the opposite of a thermophile?

Thermophiles, heat-loving organisms, have been a popular topic of research for decades due in large part to the utility of their enzymes in various chemical reactions (Taq Pol single-enzymedly made ...
9
votes
1answer
114 views

What determines when a virus becomes a “new strain”?

The reason we cannot find a cure for the common cold is because viruses mutate rapidly. Where do the mutations occur in the DNA/mRNA of viruses and does this have any effect on the protein level? what ...
1
vote
2answers
163 views

Can someone identify this bird?

We recently found a nest in the back garden and a little bird inside. Can you help us identify it? The nest is quite small like the bird so we are wondering whether this is the parent or a baby chick. ...
32
votes
4answers
1k views

Can scientists create totally synthetic life?

This particular question has been of a great deal of interest to me, especially since it dives at the heart of abiogenesis.
12
votes
1answer
6k views

What is the significance and method behind Ramachandran plots?

My PI showed a Ramachandran plot in class today with minimal explanation, but I'm interested in finding out more. I understand that the Ramachandran plot shows the relation between the omega phi and ...
4
votes
2answers
100 views

Is it possible to grow any kind of plant soilless (hydroponics)?

Hydroponics is a subset of hydroculture and is a method of growing plants using mineral nutrient solutions, in water, without soil. [wikipedia] My question is if is it possible to grow any kind of ...
10
votes
1answer
126 views

What advantage would the initial 'donor' in horizontal gene transfer by conjugation have received?

I am struggling to think why horizontal gene transfer between bacteria would have persisted during the course of evolution as surely it puts the 'donor' at a disadvantage? For example, consider a ...
10
votes
2answers
295 views

What is the highest competency possible for E coli?

I am looking to find a highly competent E coli strain. I am making a library of a ~6.6kb plasmid and I am not getting high enough efficiency. Does anyone have a suggestion of a strain/protocol with ...
2
votes
1answer
56 views

Measuring the density of surface antigens

I'm trying to get a sense of the variety of methods used for determining the number of cell surface antigens and receptors. This is notably different from determining the affinity of these surface ...
9
votes
3answers
378 views

Source of DNA sequences

I'm working on a project where I am taking DNA sequences and translating the codons into musical notes. I have some good ideas of how to do this, I'm just not sure what sequences to work with. My case ...
8
votes
2answers
171 views

Is there a dominant gene for right-handedness?

Has there been any definitive research about handedness being genetic? Also, why is right-handedness clearly dominant in humans? I'm interested in evolutionary theories, as well as any molecular ...
13
votes
5answers
317 views

Can species back-evolve?

One of the tenets of Darwin's theory is the survival of the fittest, ie adaptation of features that allow a species to adapt better to its surrounding environment. I am wondering that given the right ...
16
votes
1answer
442 views

How did viruses come to be?

My question is out of curiosity and got me thinking. How did viruses with the head, tail and tail fibres actually evolve? These viruses look more like machines than biological entities. Are there any ...
6
votes
3answers
336 views

What's the use of DNA sequencing results?

Suppose I sequence DNA of some organism (a human perhaphs) and now I have the entire DNA "string" - the sequence of nucleotides. What's the use of that? It's just a "string" where nucleotides encode ...
25
votes
4answers
1k views

What exactly are computers used for in DNA sequencing?

I've thoroughly read the Wikipedia article on DNA sequencing and can't get one thing. There's some hardcore chemistry involved in the process that somehow splits the DNA and then isolates its parts. ...
6
votes
3answers
430 views

Protein Biology Cheat Sheet

I'm looking find a cheat sheet for protein biology to stick on my desk to remind of some of the key principles of protein biology. I seem to remember the specific and complex principles in protein ...
5
votes
3answers
125 views

Measuring reverse transcriptase activity in E. coli

Goal: To quantify the HIV-1 reverse transcriptase (RT) activity for a number of mutated RTs. Idea: Put RT into a vector in E.coli. Deliver GFP mRNA to E.coli. RT will process the mRNA. The ...
16
votes
3answers
268 views

How is the blood volume of a living organism measured without killing it?

How is the blood-volume of an organism measured without killing it? NOTE: The blood-volume of an organism is defined as the total volume of blood present inside that organism.
6
votes
4answers
933 views

What's the aim of genetically modifying of foods/organisms?

On news, articles etc. experts talking about Genetically Modified Foods and Organisms often mentions about their disadvantages like, their potential to harm human health allergies may become more ...
-3
votes
1answer
314 views

How To Avoid Macro-Evolution Confusion? [closed]

I regularly encounter students who believe humans came from amoebas and when asked why they often say Macro-Evolution has been scientifically proven. Macro-evolution is defined as evolution at or ...
7
votes
1answer
112 views

Is it the sphincter that flexes when a human pushes a bowel movement?

Of course the sphincter muscle is at the exit point. To use a toothpaste tube analogy, if I want to squeeze out some toothpaste, it does me little to no good to jostle the nozzle; I need to squeeze ...
4
votes
0answers
93 views

Dimensionless number for blood volume

Comments from the question How is the blood volume of a living organism measured without killing it? by @Nico discussed that the time of blood recirculation scales with the size of the organism. I was ...
8
votes
1answer
147 views

What do we know about LUCA?

All life on Earth (bacteria, archaea, eukarya) is thought to have evolved from a common ancestor, or last universal common ancestor (LUCA). What do we know about the characteristics of LUCA based upon ...
4
votes
1answer
154 views

PCR primer in highly repetitive region

Is there any way to design a PCR primer if region of interest is highly repetitive (tens of kilobases only SINE's & LINE's)?
5
votes
2answers
162 views

First pass protein structure prediction

I'm working with a protein that doesn't have any homology to other proteins so it will likely require ab initio structure prediction. However, since I don't work for a structure prediction lab and I ...
4
votes
1answer
330 views

What's the distinction between a tetrad and a synaptonemal complex in meiosis?

What's the distinction between a tetrad and a synaptonemal complex in meiosis? Are they synonyms? I ask because the concepts seem very closely related, but it seems like there may be a subtle ...

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