2
votes
0answers
24 views

Incremental denaturation of protein mixtures

When a protein solution is heated above the denaturation temperature, it seems that denaturation does not happen at the time the temperature is reached, but it takes some time. I assumed that ...
3
votes
0answers
35 views

How to quantify the Abundance of a particular gene in Bacteria and Archaea?

For 16s rDNA metagenomic analysis, do I need to do the following steps? DNA extraction-> Purification-> PCR amplification-> Clone -> Pick every single colony-> PCR amplify -> Sequence -> ...
3
votes
2answers
94 views

Change solvent of bacterial solution

I have to perform an RNA extraction that should start with 900 uL of 2%SDS bacterial solution. The problem is that the sample is in 900 uL of PBS. I thought about centrifugation of the sample at 5ºC,...
5
votes
2answers
622 views

Why proteins are not visible on my membrane after ponceau staining?

I have a problem in western blot that I can't resolve by myself. When I am use to add 100 microgram of proteins for each sample but after running and transfer its impossible to me to see bands of ...
0
votes
2answers
584 views

Horns in animals and birds [closed]

Are there any carnivores with horns (whether extinct or currently alive)? Do any flying creatures have horns? By horns I mean horn like structure.
4
votes
1answer
133 views

Simplified molecular-input line-entry system

This may be a bit abrupt/vague question, but I didn't know how to proceed the search to get the answer. What are the properties of a 3D biomolecule which gets ignored when a 3D biomolecule is ...
3
votes
1answer
46 views

Why introduction of an extra copy of a gene related to pigmentation causes RNA interference in Petunia?

RNAi became famous after Fire and Mello experiment in C.elegans; however, it had been observed before. In the 80's, Jorgensen was trying to increase pigmentation of Petunia flowers by introducing ...
1
vote
0answers
32 views

Location of embryo development in angiosperms

What floral organ does the development of the embryos of angiosperms occurs in? Is it the ovary? The pollen tube grows down through the style and double fertilization occurs and the embryo begins to ...
4
votes
1answer
53 views

Hand-eye coordination in 3D space

A simple daily event has amazed me and triggered this question. I have read and heard about hand-eye coordination. It quite straightforward when you e.g. want to open a door or play Xbox. In this case ...
2
votes
1answer
102 views

Why do home-grown fruits tend to taste better than mass-produced consumer fruits? [closed]

It is often heard that home-grown fruits and veggies are a lot tastier than the produce found in the supermarket. Although this question may contain a skeptics element in it, it is so often heard ...
6
votes
1answer
65 views

Can the absorption of vitamins be enhanced?

Are food additives like vitamins better absorbed when you mix them among fruits? I heard that secondary substances in fruit promote the absorption.
3
votes
1answer
325 views

Cationic lipid mediated transfection optimization for 150mm dishes

I need to optimize a transfection protocol to transiently express a plasmid encoding a chimera of eyfp attached to the c term of a Golgi apparatus signaling molecule) in hela cells and hepg2 cells ...
1
vote
0answers
90 views

How do BCAA's promote faster muscle recovery?

I am aware that ingesting Branched chain amino acids (BCAA's) prior, during, and after workouts has an effect on muscle recovery due to their difficulty of metabolism. However, how much more ...
2
votes
1answer
65 views

DNA polymerase in PCR (polymerase chain reaction)

Can the DNA polymerase in PCR (polymerase chain reaction) recognize both DNA and RNA for use them as template? I want to know is it possible if my primers bind to an contaminant RNA and then any DNA ...
4
votes
2answers
189 views

Are Gram negative bacteria classified as such because of their negative membrane potential?

Does the membrane potential usually quoted for Gram negative bacteria (e.g., E. coli) refer to the potential across both membranes? - If yes, then does the potential fall more over the inner or outer ...
3
votes
2answers
8k views

Possible genotypes for blood types?

If I am blood type B, what are all the possible genotypes that could be expressed by my parents? I think it might be 16 but I was reading online and saw this: Similarly, someone who is blood type ...
2
votes
0answers
26 views

Can encoding of miRNAs in introns not cause dysregulation of gene expression?

For example, consider a gene X which has an intron which encodes a miRNA that downregulates expression of another gene Y. When the gene X is translated, this miRNA will appear as a byproduct of ...
6
votes
2answers
68 views

Can a woman possessing 1 mutated allele show attenuated color-vision deficiency?

Women possess two X chromosomes. However, during development, when the embryo has about 32 - 64 cells, one of these chromosomes is randomly inactivated (in each cell) by an lncRNA named Xist. As a ...
4
votes
1answer
395 views

Are free-nuclear division and endomitosis the same?

As far as I understood it, both are cases of karyokinesis, not followed by cytokinesis.
1
vote
1answer
58 views

Homology ratio comparison between different species

I found the following homology ratio: protein coding genes of the organism with an homolog in mouse / all protein coding genes in the organism I have done this for all protein coding genes between ...
4
votes
1answer
104 views

Can other animals see black differently?

I am not sure if this is the right place for this question, but this is a debate that has been going on between two colleagues for days and I need a resolution because it's driving me crazy. So any ...
3
votes
3answers
150 views

Can bioluminescence be used for cancer or tumor detection? [closed]

What diagnostic applications, if any, are there in using bioluminescence to detect cancer or tumors (in vivo)?
5
votes
1answer
92 views

Mathematical models of lineage selection

I'm interested in the concept of lineage selection (Aboitiz, 1991) as an explanation for why traits would be selected for that enhance the rate at which evolution can occur, rather than directly ...
1
vote
0answers
16 views

Steps in making mass/volume concentrations? [duplicate]

[TLDR When making mass/volume concentrations, is the general workflow to make a stock and dilute from it to yield the proper diluted concentrations? Could anyone detail their methodology on the topic?[...
2
votes
1answer
28 views

Is it possible to accurately measure micronutrient levels with the blood volume from a Dermal / Capillary puncture?

I have not been able to find much information beyond what tests are normally done with Capillary puncture sampling, as opposed to what tests are possible. Could we test for a single micronutrient, or ...
3
votes
1answer
106 views

What color was the most recent common ancestor of all swans?

Was the most recent common ancestor of all swans black, or white, or some other color? How do we know, and how certain can we be?
2
votes
2answers
638 views

Using an analytical balance (0.0000 precision) to measure µg (micrograms)?

If i needed to prepare 200µg/ml of proteinase K, and the proteinase K was in a solid powder form, would I have to weight out 200 µg using an analytical balance, and if so, is it possible with a ...
1
vote
0answers
24 views

Is this a legitimate teratoma for a human pluripotent stem cell assay?

I can't quite make out this teratoma:                            ...
3
votes
2answers
96 views

Sequence compression method / format

As someone working with sequencing data and sequence files I was wondering whether is there a compression method / format for sequence / read files? Of course one can use general compressors like rar ...
-1
votes
1answer
2k views

How to make µg/ml concentrations of proteinase-K?

How does one prepare concentrations in the mass/volume (weight/volume) form, for substances like nucleic acids or in this case, proteinase? A detailed example would be helpful. I need to prepare ...
5
votes
2answers
707 views

What percentage of a cell's volume is occupied by protein?

I was looking at one of David Goodsell's illustrations of a cell: And it seems to suggest a very crowded picture of the intracellular environment. Just how crowded are cytoplasms? What percentage ...
1
vote
0answers
61 views

Primer Designing [closed]

I have a basic idea about the requirements in primer designing. Basically my knowledge is limited to theoretical knowledge and have no experience in actual primer designing. And also I have a basic ...
2
votes
1answer
301 views

Is the Klein's bottle a good analogy to the relation between T-tubule and sarcolemma? [closed]

I am not quite seeing how the T-tubule and sarcolemma is connected. It says that the T-tubule is an "invagination" of the sarcolemma, which is sarcolemma folded from the inside to form a T-tubule Can ...
2
votes
1answer
175 views

Can silverfish climb and produce silk?

I am equally disgusted and fascinated by the "Silverfish" (Lepisma saccharina) that we regularly find in our UK home. I tried searching for information on the internet but they seem poorly understood. ...
3
votes
1answer
291 views

Examples of Vestigial Genes

I consider the existence of a broken GULO gene in humans to be the clearest evidence for human evolution - just what else is it doing there otherwise? Are there any other examples of vestigial genes ...
8
votes
0answers
293 views

Contact Inhibition of Cell Division: Signaling Pathway

The following article refers to contact inhibition of cell division in epithelial cells, specifically MDCK cells: Collective and single cell behavior in epithelial contact inhibition. In their review ...
1
vote
1answer
63 views

How detachment/separation works in biology?

It might be a strange question, but I'm interested in the mechanics of separation/detachment during asexual reproduction, for example when an organism reproduces by budding (I don't mean cellular ...
1
vote
0answers
48 views

How much of the biological research can be still run without high-cost high-precision instruments? [closed]

To me it seems like much of the biological research today requires an array of expensive devices before it can be run. How much of the current research could be still done with a basic compound/stereo ...
3
votes
3answers
1k views

How to increase the shelf life of yogurt without refrigeration?

When we make yogurt at home and do not refrigerate it, it will become sour because of conversion of lactose into lactic acid by Lactobacillus bacteria, but this does not happen in case of Nestle's ...
5
votes
0answers
107 views

How does an organism know when to stop growing, whether it is already on the outermost layer? [closed]

When a multicellular organism is under its development how does the current outermost layer of cells "know" that they should not divide furthermore, as their bodyplan coded into DNA is already ...
4
votes
1answer
238 views

How pain can stimulate the vagus nerve

I'm trying to find out why a prompt, severe, short pain is causing a stimulation of the vagus nerve. What could the physiological explanation be? Is that because the pain is triggering the ...
3
votes
0answers
173 views

How to get only perfect matches with blastn? [closed]

I have installed Blast locally and I've configured the nucleotide database to use. I want to search some sequences (from a .fastafile) in this genome. So I used blastn: ...
18
votes
2answers
3k views

Is it known how the first viruses formed?

The oldest known virus is known to have infected prehistoric insects 300 million years ago. A virus is basically a parasitic strand if DNA or RNA encapsulated in a protein coat. It enters cells by "...
5
votes
1answer
76 views

Genes-of-interest analysis between organisms

I am interested in identifying the differential expression between several genes-of-interest of different organisms. I am trying to assess the benefits of RNA sequencing over microarrays. Both ...
8
votes
4answers
1k views

Is there any way a human could whistle and be unable to speak?

Is there any situation anatomically, where a human could understand the speech of others perfectly, without any capabilities of speech themselves, but would retain the ability to whistle with a tune? ...
0
votes
2answers
28 views

Risk of autosomal dominant disorder [closed]

What can you conclude about the risk (with each pregnancy) that a couple could have a child affected with an autosomal dominant disorder if... One parent is affected (heterozygous) and the other ...
-2
votes
1answer
172 views

Blood Type/Punnett Square [closed]

Male: Blood type O (Genotype written as: i^o i^o since O allele is recessive to the A and B alleles). Female: Blood type AB Draw a punnett square to show all of the possible offspring. I got Ai^o, ...
3
votes
1answer
194 views

Why are skin tactile receptors considered to be phasic receptors?

A phasic receptor by definition is one that quickly adapts to change. In terms of neuron firing, this means that it does not trigger another firing because the receptors are used to the stimulus. But ...
3
votes
1answer
94 views

How to determine genotype?

In humans, the ability or inability to roll the tongue is a single gene trait. The allele for tongue rolling (R) is dominant to the allele for not being able to roll the tongue (r). Whether or ...
2
votes
1answer
62 views

Serious question about a piece of meat

Does anyone know some scientific rigorous way to look at a slice of meat. I am not asking what kind of meat it is or which part of the animal it came from. I wonder what is the different lining on the ...

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