6
votes
2answers
161 views

Effect of single-gene overexpression in the cell's response

Which are the factors that modify the overall gene differential expression by introducing a vector for single-gene overexpression? If you overexpress a gene for a protein involved in signal ...
5
votes
1answer
562 views

Is there a relationship between efficiency of cellular metabolism and warm-blooded-ness?

My BIO 101 book states that when human cells convert glucose to ATP, the process is only approx 35% efficient, and much of the potential energy is lost as heat. However, that heat is useful to us in ...
6
votes
1answer
170 views

Understanding Membrane / Resting Potential from the perspective of ions?

From wikipedia article RESTING potential: "there is no actual measurable charge excess in either side. That occurs because the effect of charge on electrochemical potential is hugely greater than ...
11
votes
1answer
1k views

Why do we like salt?

A few friends of mine told me that salt provides zero nutritional value to us, and in fact can harm our bodies. Now, these guys are medical students, and being an engineering student myself, I decided ...
5
votes
1answer
137 views

Which cells are responsible for the extracellular matrix remodeling?

I am studying a case of tendinopathies induced by an alteration of how the extracellular matrix is being remodeled. From my understanding there has to be a careful balance of MMP (metallproteinases) ...
3
votes
1answer
323 views

Does caffeine increase the speed at which sperm travels?

I'm not even sure if this claim is true, but... This source states that Caffeine gives both types of sperm a boost, but the Y-sperm would get a little more of a boost. This article reinforces ...
6
votes
1answer
350 views

Are the cytosol and extracellular fluids electrically neutral?

I've found several sources that state that overall, the cytosol of a cell is electrically neutral. The extracellular fluid is also purportedly electrically neutral. How can that be when we have ...
6
votes
2answers
752 views

What is the correct model for enzyme-substrate complementarity?

This Wikibook shows both proposed models of enzyme-substrate complementarity, the Lock and Key model and the Induced Fit model. I've always been taught that the Induced Fit model is the proper one. ...
17
votes
1answer
895 views

Is kissing a natural human activity?

The word natural here is meant in contrast to it being a sociological construct. Is kissing in all its forms something natural for humans? Is it instinctively erotic? Or is it just a conventional ...
3
votes
1answer
313 views

Troubleshooting bioconjugates migration in a SDS-PAGE gel?

We do a lot of bioconjugation chemistry (click chemistry in particular but also NHS and Maleimide chemistries). Our method to valid the conjugation reactions have been to use SDS-PAGE gels followed ...
8
votes
1answer
260 views

Are there dog breeds that are so far apart genetically that they can't produce viable offspring?

Obviously, a very large dog would have difficulties mating with a very small dog and vice versa. But putting that problem aside (using, say, insemination), considering the large variation of dog ...
3
votes
1answer
187 views

T7 phage promoter action in mammals

Does anyone know if the T7 phage promoter is efficient in mammalian systems?
4
votes
2answers
1k views

Online course on Plant Taxonomy and Physiology

After following a course in Permaculture, I realised that if I want to get serious about it, I need to be able to distinguish plants. So I am looking for an online Video course on Plant Taxonomy and ...
3
votes
0answers
48 views

Is there a gene that starts meiosis 2? [closed]

Yesterday I thought about a question and asked it to my friend. The question was which gene is completely the same for a male cell that made meiosis 1 recently. My answer was the gene that starts ...
5
votes
2answers
239 views

Energy use by muscles, actual work done by muscles and more

Lately, I've started exercising in the gym and outside. I've also started to look at the details of food I eat. Food usually has a label saying the amount of energy is inside it. For example, some ...
1
vote
1answer
186 views

Which concentration of BSA is recommended for dynamic light scattering experiments?

What is the recommended concentration of BSA to be mixed with water so as to prepare a very dilute solution in order to clearly study the intensity-intensity correlation with single scattering ? I ...
5
votes
1answer
151 views

Is there such thing as Animal non-verbal body language?

Have there been any studies done on the animal use of their bodies to signal, communicate or express their emotions, particularly to members of other species (ex: humans)? I've been observing a very ...
9
votes
2answers
146 views

Modularity of transcription factors

I attended a seminar about neurogenesis that presented results for PAX6 as an important TF that contains 3 domains with very distinct patterns of downstream expression. The speaker ended up saying ...
6
votes
1answer
328 views

How can I predict DNA binding affinities from a protein sequence?

Are there any computational tools to predict the binding affinity to specific DNA motifs from protein domain sequence information?
2
votes
1answer
213 views

Are Bovine serum albumin, Avidin, Ficoll-70 and Dextran-70 positively charged or negatively charged? [closed]

Bovine serum albumin, Avidin, Ficoll-70 and Dextran-70,are they positively charged or negatively charged ? And which other solvents can be used as a substitute to water for preparing solutions in each ...
8
votes
1answer
549 views

What causes knuckle “popping” and the feeling of relief that comes from it?

Many people like cracking their knuckles. I have always been curious about it. What happens when we crack knuckles? What creates the POP sound? Why do we get a relief-like feeling after doing it?
4
votes
1answer
132 views

What biological systems are affected by Vitamin B6 (or B vitamins in general)?

B vitamins and particularly the B6 vitamin appears to the one of the main ingredients in energy drinks. There has been anecdotal evidence from people interested in dreaming that ingestion of vitamin ...
4
votes
2answers
168 views

Is there a good field methods book that covers terrestrial ecology?

In the past I have used Limnological Analysis by Wetzel and Likens and Methods in Stream Ecology edited by Hauer and Lamberti to develop labs and research methods for courses and projects with an ...
4
votes
0answers
205 views

How do I save a coffee damaged wasp? [closed]

I found a wasp in one of my coffee cups about 1 hour ago. There was barely any coffee left but it had managed to get itself pretty much drenched in coffee. I took it out. Put it on a piece of paper ...
8
votes
2answers
84 views

Is there a Reverse Transcription optimization for long, 9kb, transcripts?

Has anyone optimized RT for long transcripts (9kb)? The downstream application will be PCR amplification and Illumina library prep. It will be trivial to make internal primers sets for the PCR that ...
0
votes
1answer
101 views

What impacts on metabolism are likely from the 6x increase in water consumption by Americans at restaurants since the 1950s?

CDC data show that American water consumption in restaurant meals is 600% of what it was in the 1950s while meal calorie density has decreased (kcal/oz). What impacts is this likely to have on the ...
6
votes
2answers
109 views

phylogenetic analysis of gene enrichment?

Are there any tools to do phylogenetic analysis of gene enrichment? This is, I have a list of genes from an experiment performed in several species, with a z-score that can be described as ...
5
votes
1answer
182 views

Formal definition of a 'genetic trait reservoir'?

I've read the tomato genome paper in Nature published recently, and they describe the tomato genetic resource as a rich trait reservoir that will provide biological ...
3
votes
1answer
248 views

Why apes started to contemplate and become altruistic? [closed]

I was asking this first on philosophy forums, but it is suggested, that answer could be found from evolutionary view of point, so here we go: Again my question raised when thinking of myths, their ...
8
votes
3answers
2k views

Is it better to take a half dose of paracetamol and a half dose of ibuprofen together rather than a full dose of either?

Recently, I heard on this health-related radio programme that it was better to take a half dose of paracetamol and a half dose of ibuprofen together, rather than the full dose of either one, for acute ...
5
votes
2answers
52 views

tools to reconstruct the transcriptional regulatory circuits?

What are commonly used tools to reconstruct the transcriptional regulatory circuits that govern diverse cellular responses and what input data sets do they accept?
7
votes
1answer
145 views

What makes a wet dog so stinky?

Similarly, what makes a wet down comforter so stinky? Why do they make no smell except when liquid is added?
6
votes
2answers
192 views

Can I compare Shannon indices of metagenome gene data?

I'm comparing 12 Metagenomes. I'm using HMM counts of a number of proteins known to exists as groups within certain operons. I have grouped the HMM counts for each type of operon and calculated the ...
8
votes
1answer
424 views

How many gigabytes of DNA are there on earth?

The human genome is about 770 MB, the C. elegans genome is about 100 MB, the yeast S. cerevisiae is about 12 MB. Different other genomes have been sequenced: how many GB of genomic DNA we have now? ...
7
votes
1answer
192 views

Why are some menstrual cycles irregular?

A friend of mine asked me this today, and I didn't know. I remember studying the menstrual cycle a few years ago, but I can't remember the details (other than it was very hormone-related), and so I ...
4
votes
1answer
502 views

Why are omega-3 fatty acids so easily oxidized when they're incorporated in cellular membranes?

Apparently, this has led to results with clinical significance, as we can see at http://extremelongevity.net/2011/10/03/daily-fish-oil-consumption-may-reduce-lifespan/... The researchers fed a ...
5
votes
1answer
95 views

Reproduction and defecation

In mammals and birds the external reproductive organs and waste removal organs see to be the same. Is this always the case with other animals such as insects etc...? Are there any good reasons for it? ...
6
votes
1answer
44 views

harvesting fertilized eggs from Tetraodontidae species?

I would like to know how easy/difficult it is to harvest fertilized eggs from Tetraodontidae species such as Tetraodon nigroviridis or Takifugu rubripes compared to zebrafish? Ultimately, I would like ...
7
votes
1answer
923 views

Is it possible for any animals today to have more than one brain?

Is it possible for any organisms in the animal kingdom to have more than one brain?
5
votes
2answers
485 views

Did dinosaurs have more than one brain? If so, why?

I once remember reading (15 years ago) that dinosaurs had two brains. One for their head and another one for their digestive functions. What is the current opinion on this theory? Has more evidence ...
5
votes
1answer
1k views

Do hot drinks cool you down?

It is quite the old wives tale that drinking a hot drink cools you down. If you don't really think about it it does seem somewhat logical: increasing temperature will cause your body to try and cool ...
4
votes
1answer
240 views

Can humans shorten their sleeping patterns?

Can sleeping patterns be trained, or are they genetic? I read that a human needs to sleep at least 4 hours. Can sleeping time be reduced without having a negative impact?
3
votes
0answers
24 views

What metabolically happens when an egg fuses with the nucleus of a somatic cell

In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
2
votes
2answers
180 views

How might I break down bread into glucose in a model of the human digestive system?

I need to make a model of the digestive system. It would be really nice to actually show it in action, such as by breaking down bread into glucose or something similar. Is this feasible with a small ...
0
votes
1answer
183 views

What is the similarity between how cells organize themselves to form a human and how humans organize themselves to form a society? [closed]

Some things I have gathered. Common properties - individuals - communicate - local view - selfish - specialize - organize - replicate - cooperate - emergence Terminology cell ...
4
votes
1answer
144 views

Vigorous shaking for HFR interrupted mating

I am trying to reproduce E. coli interrupted mating using an HFR strain, and I read that the cultures should be vigorously shaken at times to interrupt the DNA transfer from the F+(HFR) donors to the ...
0
votes
1answer
118 views

Herbal vs Chemical Insecticides [closed]

Well, insecticides are used to kill insects. I have always wondered why are herbal / natural insecticides more effective in killing insects than chemical ones. I have read this at many places but ...
5
votes
1answer
168 views

What is an simple way to burn glucose for visible effect?

I want to make a partially working model of the digestive system that could digest complex carbohydrates. My ultimate goal is to be able to cut up some bread, put it into the model, operate it, and ...
8
votes
1answer
232 views

How Antioxidants affects Human Metabolism?

I'd like to know how antioxidants affects the human metabolism and which ones are essential for metabolism processes.
6
votes
2answers
73 views

Macrophage pathogen fixation

Overly simplified, macrophages recognise pathogenic patterns and endocytose anything that matches them. That also works on bacteria, which are quite often very mobile. What if a bacterium was just ...

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