2
votes
1answer
376 views

Mesosome - does it exist?

This wikipedia page says that mesosomes are artifacts produced by chemical fixing techniques. Most of my textbooks still show a mesosome and describes it as having functions like - respiration, ...
1
vote
0answers
26 views

what are hyperbranched amplicons in DNA sequencing?

I am reading an article about single-cell sequencing: http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.2720.html And came across the concept of "hyperbranched amplicons". I googled for it but ...
5
votes
1answer
261 views

Time spent in phases of cell cycle

I am looking for references to papers containing the time intervals spent in different phases of the cell cycle (ej., G0, G1, S, G2, M for eukaryotes) for different cells. In particular, I am ...
1
vote
3answers
202 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
2
votes
3answers
240 views

Bacterial cell lysis buffer used in proteomics procedures

What kinds of detergent-free bacterial lysis buffers exist? The proteins we're extracting will be later analyzed by LC-MS/MS, and we're looking for a lysis buffer that won't interfere with this ...
2
votes
2answers
135 views

Interdependency between organsims on our planet and its relation to ecosystem?

I am not a biologist but I just wonder if all species are interdependent. I mean if the tiger becomes extinct does it affect the ecosystem? If the mosquito or mouse become extinct does it affect the ...
3
votes
1answer
157 views

Empirical evidence for Group Selection?

A controversial concept in Biology "Group Selection", has caused confusion and conflict amongst scientists since the since the mid 1990s. The more general realm of study is termed the "unit of ...
1
vote
0answers
169 views

On the definition of gDCW (gram dry cell weight)

I need some clarification on the concept of gram dry cell weight. The unit acronym gDCW stands for gram dry cell weight. My interpretation is that 1 gDCW is equivalent to 1 gram of dry cells, but I ...
1
vote
1answer
2k views

Is it safe to look at infrared LEDs?

What happens if one looks directly at infrared LEDs? Sometimes I see this kind of LED at night as red dots and I'm also courious about it. I was wondering what would happened if one looked directly ...
3
votes
2answers
182 views

Are these cats calico?

Calico cats are cats that have 3 fur colors and are always female or males two X chromosomes. I've seen many cats that will have 2 fur colors (one of them is usually white) and another, third, color ...
1
vote
2answers
248 views

Is the SRY gene the sole responsible of biological male sex?

It's very well known that the Y chromosome is what determines maleness, but more specifically this seems to happen thanks to the SRY gene located on it. Some individuals have an XX karyotype, but ...
4
votes
2answers
78 views

Question about the expression of MHC

Is it possible to express both the MHC class-1 and MHC class-2 both in one cell?
11
votes
2answers
305 views

Why, specifically, does each generation, on average, improve upon the design of the species rather than degrade it?

In every non-life example I can envision, a copy of a copy is always a degraded or less pure version of the original unless some outside influence acts to correct the copy back toward the ideal ...
4
votes
2answers
87 views

Do all humans experience flatus regardless of their diet?

Flatus frequency is affected by diet, so I was wondering if flatus production solely depends on the food eaten, meaning that some people never ever release flatus because they don't eat any ...
2
votes
1answer
82 views

Determining sequence of oligoribonucleotide

Oligoribonucleotide X was treated with phosphatase (for removal of 3' and 5' - terminal phosphates), then with RNAase T1, which cleaves all phosphodiester bonds located in a 3' position of ...
0
votes
1answer
196 views

What are the universal laws of Nature concerning animals' behaviour? [closed]

I'm looking for very old (centuries) treaty or recent (20 & 21th century) research that state what some of the laws of Nature are, particularly concerning wild animals' behaviour. Such as; every ...
1
vote
0answers
1k views

Bacterial 16S rRNA PCR amplification with universal primers

I am doing an experiment to amplify the 16S rRNA gene from bacteria present in gut contents of fish. After extracting DNA, I perform one-step PCR with universal bacterial primers (27F, 1492R) and I ...
3
votes
1answer
188 views

Do genes play a role in the athleticism and shape of a person?

I am an Indian with a small thin stature with some deposition of fat around my belly.I was wondering if genes play a role in fat deposition in the body for Indian, Chinese, European or African people. ...
4
votes
4answers
298 views

Biodiversity is restricted by genome combinatorics?

Me and some friends are interested in opinions for the following: Conjecture The maximum number of species must be limited by the maximum combinatorial/permutational space that can be occupied ...
4
votes
1answer
118 views

Natural enemies boost deep evolutionary fitness?

"Two species that reduce one another's fitness on microevolutionary (short-term) timescales can increase each other's macroevolutionary (long-term) fitness" Opinions? Intuitive Examples: ...
4
votes
1answer
85 views

What makes alcohol harmful to mammals?

During the alcohol metabolic pathway, harmful by-products are made like acetaldehyde, hydroxyethyl, superox­ide anions, and hydroxyl radicals. How do these toxic compounds harm our tissues? Many of ...
5
votes
1answer
493 views

Could alcohol be used as an antibiotic?

I was thinking about how alcohol is used to sterilize, and I was wondering if it would be possible to use alcohol as an antibiotic? if it kills bacteria, couldn't (conceivably) alcohol in the ...
3
votes
1answer
699 views

Problem with bacterial transformation with electroporation

I have a problem with a bacterial transformation of a yeast gene that I can not solve. I isolated yeast DNA and did a PCR to get my product. I am using pCGCUm vector with a GFP construct. I digest ...
1
vote
2answers
39 views

Regulation of the replication of mtDNA at embryonic level

While reading an article on mitochondrial inheritance I came across this link. The results state that mitochondrial DNA replication is regulated in different cells of an embryo at different levels. ...
1
vote
1answer
273 views

What is the most recent non-primate ancestors of humans?

If we go down the evolutionary tree of humans, (Homo sapiens, Homo erectus, ...), what will be the first non-primate ancestors?
3
votes
1answer
53 views

Are there cannibalistic k-selectors?

My understanding of r- and k-selectors does not go deeper than the wikipedia article. I understand k-selectors to be creatures that put tendentially more effort and energy into rearing their ...
3
votes
2answers
1k views

Are Italians genetically separated from other Europeans?

I was reading this Wikipedia article about the genetic history of Italy, and I found it interesting. There are however a few things that puzzle me, because they seem to contradict each other. For ...
3
votes
1answer
80 views

What's a replicate line?

The methods section inside a paper I'm reading make mention of replicate lines. Example: "We founded 10 replicate lines from a single clone". This is in the context of experimental evolution and ...
1
vote
2answers
1k views

Definition of “Niche Complementarity”

What does "Niche Complementarity" means? On this website they define "Niche Complementarity" as: The tendency for coexisting species which occupy a similar position along one niche dimension ...
2
votes
0answers
69 views

What are the ingredients of Pheromone Trap using for controlling Fruit flies of cucumber?

Pheromone Traps are used for fruit fly control. But I have no idea which ingredients or chemicals are used for preparing Pheromone trap
2
votes
2answers
4k views

Why aren't we allowed to refreeze previously thawed breastmilk in refrigerator?

https://www.breastfeeding.asn.au/bf-info/breastfeeding-and-work/expressing-and-storing-breastmilk Previously frozen— thawed in refrigerator but not warmed 4 hours or less (ie the next ...
-1
votes
1answer
23 views

Order of events in hibernation

Arrange this in sequence : i. Heat loss exceeds heat production. ii.As body temperature falls, heat loss decreases. iii.Body temperature equals environmental temperature. ...
0
votes
1answer
23 views

Is ability to easily pollinate desirable?

In Mendel's crosses was it desirable for the plants to be able to cross-pollinate and self pollinate easily ?
1
vote
0answers
30 views

Where can I get yeast mutants? [duplicate]

I want to use some yeast mutants to conduct gene functional analysis, and the gene was isolated from plants. Can anybody give me some information regarding the websites or agencies from which I can ...
3
votes
0answers
50 views

Why is this dry pepper fruit still “powered”?

Check out this photo of my pepper plant this cloudy morning: (click for full res) Usually (and this can be seen on 2 and a half of the 4 fruits in this pic) the fruits are "disconnected" from the ...
1
vote
0answers
64 views

3' or 5' DNA capping protein

Does anyone know of a protein/molecule that can be attached to one end of the DNAs temporarily ? eg. i would like to only to allow one strand of DNA be ligated to another DNA molecule to either it's ...
1
vote
2answers
173 views

Do people often blow out the air from their lungs a lot when living in the cold condition to keep their lungs warm?

In a cold climate, do people often blow out the air from their lungs a lot when living in the cold condition to keep their lungs warm? I asked this question because it could connect to how people in ...
6
votes
2answers
95 views

How predictably will RNA polymerase not transcribe repetitive sequences?

I am working on an (expensive) synthetic construct, which happens to have many "repetitive" sequences within it that are integral to its function. Primarily, the two sequences that are worrying me ...
2
votes
0answers
52 views

What are these fleeting sea worms? [closed]

I filmed the creatures below this August in the Mediterranean (specifically, the port of Fornells in Minorca of the Balleares). The images were taken from a boat moored just outside the harbor and ...
5
votes
1answer
57 views

What limits our ability to grow enamel rods?

From what I've understood from what I've read (which might be wrong), enamel rods extend away from the dentin in our teeth. As young people grow, the rods are thickened. What are the technical ...
2
votes
0answers
33 views

How has the use and subsequent ban of DDT affected insect populations?

Living in Australia there were less ticks for example during the use of DDT, now there are more ticks prevalent here. I am wondering if any insect species have declined permanently as the result of ...
0
votes
1answer
1k views

For the human head, which direction is anterior, and which direction is ventral?

I read the wikipedia entry and I am sort of confused. In dog, the nose is toward the front. That's anterior. So I conclude that human nose is anterior. In dog, the head is on the back. So that's ...
0
votes
1answer
1k views

What is the difference between SNP and STR?

I thought that these were just different format of the same data. But it seems there isn't a way to convert SNP (single nucleotide polymorphism) data to STR (short tandem repeat) data. Am I right? ...
2
votes
1answer
65 views

Does the existence of one species imply the existence of many other?

Reading one of Sir Arthur Clarke's scifi stories, I came across a statement something to the effect of, existence of a single species automatically implies the existence of many other species. So ...
1
vote
1answer
120 views

Hypothetically, what is the smallest functional form of human?

Since the my first encounter with doraemon manga, I've been wondering many things, one of them is the small light. Assuming we had an instrument, such as small light, which enabled people to grow and ...
3
votes
1answer
102 views

Where does all the carbon go?

I found it hard to get numbers on global primary production, but apparently it's somewhere around 50-60 $Gt / year$ for carbon content in the terrestrial production, and a similar figure for the ...
15
votes
4answers
2k views

Why would a single celled organism evolve to be multi-celled?

I read a story this week on Richard Lenski who has been 'evolving' E. coli for more than 50,000 generations now. One comment I read was from someone who doesn't accept Evolution who pointed out that ...
1
vote
2answers
515 views

Why doesn't T5 exonuclease have endonuclease activity in Gibson assembly?

Gibson assembly uses T5 exonuclease to chew back the 5' end of dsDNA to generate overhangs. However, T5 exonuclease, in contrast to lambda exonuclease, is reported to have ssDNA endonuclease activity ...
2
votes
1answer
84 views

Identification of this plant?

Photographed in Rocky Point, Mexico. Any ideas about this plant? Thank you!
4
votes
1answer
218 views

What is the evolutionary advantage of regret?

Some motivational speakers may say that regrets are only useful to learn something from. What are the other advantages?

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