1
vote
1answer
171 views

Can PolyA tails be within Expressed Sequence Tags?

Can polyA tails occur within (rather than at the end) of a sequenced tag? Consider, for example the following two sequences from NCBI: DY008075 ...
0
votes
1answer
63 views

Some question about materials for PCR

I'm planning to order oligo, polymerase, nucleotide, buffer for my first diy PCR experiment (now I have nothing), so I have some questions. How and how long I can storage them? I have only a fridge ...
6
votes
3answers
2k views

Why do we squint when tasting very sour things?

Sometimes while tasting a very sour thing (like tamarind, lemon etc.) our eyes squint immediately and involuntarily for a second, but a little later becomes normal again. Why, and how, does this ...
1
vote
1answer
364 views

How different is an introverts brain different from an extroverts?

How is an introverts brain wired differently than an extroverts? Just to give you an example. On an average, the processing that goes on in an introverts brain is greater than in an extroverts ...
0
votes
1answer
58 views

Carotenoid biosynthesis in yeast

Does Budding Yeast Sacchromyces cerevisiae produces significant amount of carotenoids? Have anybody estimated the ratio of flux going in branches 1. Cholesterol synthesis(via squalene) 2. Coenzyme Q6 ...
5
votes
2answers
217 views

Can PCR tubes be made of different materials than plastic?

Does it matter if I replace the PCR tube (usually made of plastic) with another material like aluminum, glass, or something similar?
2
votes
2answers
278 views

Can a dNTP be built into a RNA strand?

DNA consists of deoxyribonucleotides, RNA consists of ribonucleotides. They differ mainly (apart from the uracil / thymine difference) in the sugar part, the deoxyribose and the ribose. Those two ...
0
votes
1answer
326 views

Confusion regarding photosynthesis and respiration in plants

During 24 hours there is a time in i.e. twilight when plants neither give oxygen nor carbon dioxide why is it so? This also suggests that neither of the two vital processes i.e. respiration or ...
3
votes
1answer
127 views

Method to recognize the age of a firebug from the pattern on its back

Can I recognize the age of a firebug from the pattern on its back? If yes, what pattern shows what age/stage? Below is what I extrapolated from Wikipedia, but is there a more precise scale with more ...
1
vote
0answers
59 views

Which are the most extreme artificial environments that lifeforms/ecosystems have adapted to?

I have heard of 'extremophiles', lifeforms that naturally thrive in all sorts of extreme environments. Is there any evidence to suggest that lifeforms are adapting to the extreme environments (such ...
1
vote
1answer
46 views

What Effect would Cannabinoids have on the Acute Porphyrias?

I should be clear in saying that this question is NOT intended for personal medical advice. Rather what I am looking for is journal articles and/or books that touch on this subject because I think it ...
4
votes
2answers
775 views

How does ubiquitin recognize misfolded proteins?

Misfolded proteins are tagged by ubiquitin and then are destroyed by proteasomes. How does ubiquitin know which protein to tag?
5
votes
1answer
72 views

Selective Androgen Receptor Agonist

I'm looking for an inducer that strongly activates the androgen receptor, but not the glucocorticoid receptor that is not DEA regulated. I know that SARMS (Selective Androgen Receptor Modulators) are ...
5
votes
1answer
1k views

What biological constraints are there on how large a spider can grow?

Spiders fascinate me, but what intrigues me the most is just how big some spiders can get, which leads me to my question. What biological constraints (if any) would limit how large a spider could ...
10
votes
1answer
229 views

What are the functions of magnetic bacteria?

I'm trying to understand why are bacteria "equipped" with magnetosomes and why/what for do they need such organelles.
3
votes
1answer
1k views

What insect is this? (Black body two orange lines in its back and six legs)

I found this today in the library while studying. I am very curious to know whether this particular insect has a name that I can look up and find more information about: Full length is almost one ...
1
vote
1answer
273 views

How can I create a microcapillary for manipulation of single cells?

i'm working as a diy bio. I'm finding a way to create a micro glass capillary for picking up single cells. I see this video on youtube and would like to know what is the minimum I/O diameter if I pull ...
5
votes
3answers
136 views

Finding a template/oligo combination for my first PCR experiment

I'm an information technology engineer. I love biology so I research biological topics and have an interest in PCR. That's why I have decided to create a PCR machine. Everything is done now and I ...
2
votes
3answers
109 views

What do cells do when they aren't creating proteins?

I've always thought that the majority of the "work" in a cell is protein production, until I read the following. The Wikipedia article on the central dogma of molecular biology states this: 80% ...
1
vote
2answers
366 views

Does osmosis occur when we have two different substances?

Assume that we have a semi-permeable membrane with water on both sides. First, straightforward, case: On the right side, we have a concentration of the substance A, and on the left side, we have pure ...
2
votes
1answer
10k views

What are the functions and differences between axons and dendrites?

My textbook doesn't do a very good job of pointing out what the differences between the two are. It basically mentions axons only in the same breath as the synapse (that synapses are the endings/tips ...
4
votes
2answers
476 views

Why are enzyme-catalysed reactions slower at lower substrate concentration?

Suppose I'm using 200 nmoles of enzyme and 2 mmoles of substrate. The enzyme should be saturated but if I use 50 mmoles of substrate, the reaction will be faster. Why? I just can't get it! Even at ...
0
votes
3answers
136 views

Pump to separate protein/DNA instead of electrophoresis?

I know that DNA molecules or proteins can be separated in electrophoresis because the electrical charge is used to pull the DNA through the gel. So instead of using electrical, can I using physical ...
1
vote
1answer
117 views

How potent is the venom from the Australian platypus?

The Australian platypus, is a marsupial with a unique feature - in that the males of the species have venom sacs around their hind-claws. My understanding is that this venom is not lethal to humans, ...
4
votes
0answers
154 views

Coiling of chromatids during cell division [closed]

What is exactly coiling of chromosomes? I just heard about the names i.e paranemic, plectonemic, orthostichious, anorthospiral. I have ecaxtly no idea of what phenomenon is this. Also what type of ...
4
votes
2answers
11k views

What happens to the holes in the skull where teeth were developing?

In Skeptics.SE, a question regarding the skulls of children arose. Depicting a scary skull where the permenent teeth are "hidden" inside the jaws and the milk teeth are in the their place. After all ...
1
vote
0answers
69 views

How to ligate DNA to an RNA molecule

Is it possible to ligate a small DNA molecule to mRNA? I was reading a protocol from Current Protocols in Molecular Biology and one of the procedures involve ligating a DNA molecule to mRNA using a ...
0
votes
2answers
169 views

What do you call the non-Wild Type allele?

Can I have a list of suggestions, such as the mutated allele, other allele, etc. ? Are there any blatantly missing ones on my short list?
2
votes
1answer
71 views

Aside from cockroaches and other insects, which species (of all kinds) are 'immune' to ionizing radiation?

I have just been reading this excellent question and answer about cockroaches ability to withstand ionizing radiation. My question is an extension of that question: aside from insects, which other ...
3
votes
1answer
1k views

What is the exact principle of capillary isotachophoresis?

I know it's a type of capillary electrophoresis, but I don't get how does the separation happen exactly.
0
votes
1answer
166 views

How do you remove DNA/RNA from plastics?

I want to know how to make plastic containers free of DNA & RNA? this is just for knowledge
3
votes
2answers
79 views

Why not use Cu(OH)2 as a dye for protein electrophoresis?

As I know, $Cu(OH)_2$ $+$ Protein $\rightarrow$ Purple. So why not use $Cu(OH)_2$ as dye in protein electrophoresis?
3
votes
1answer
179 views

Why Is The Toughness Of Skin Different On Different Parts Of The Body?

My cat was licking my arm with his sandpaper like tongue. It hurt and the area he was licking was slightly smarting afterwards. However, when he licks the palm of my hand the feeling is rather ...
5
votes
2answers
1k views

What is dental health like in animals?

Do animals suffer from tooth decay, such as cavities? Is it to the same extent as in humans, or do animal teeth decay more or less? If teeth decay in animals as well, are there natural strategies ...
11
votes
4answers
189 views

Is there any other function of DNA?

We all know that DNA acts as a genetic molecule. Does DNA have any other function in the cell other than being a genetic material and carrier of information?
15
votes
2answers
360 views

Can parts of a human brain be asleep independently of each other, or vary in the times required for them to fall asleep?

I know that some birds and marine animals can continue complicated activity (swimming, flying?) while one hemisphere of their brain is asleep. I'm interested if human brain has some parts of it that ...
2
votes
1answer
2k views

fibroblast cells and fibers

I am interested in fibroblast cells in human arteries. Here are the things that I am not clear at the moment and I could not find any answer from the literature: What are the dimensions of these ...
2
votes
2answers
3k views

What is a pA(2) value?

I saw this article http://www.ncbi.nlm.nih.gov/pubmed/16710314 and it mentioned pA(2) values and I had no idea what they were. What are they? What do they mean? If possible it'd be just dandy if you ...
4
votes
1answer
74 views

Why don't individuals with sickle cell trait suffer from sickle cell anemia?

Ostensibly, in people with sickle cell trait, half of the hemoglobin in their body would be defective. Is it actually the case that sickle cell hemoglobin is produced in equal amounts? If not, how ...
3
votes
1answer
108 views

Chromatin shearing: what is it and what are the effects of high-sensitivity?

I am reading a paper which discusses a complex (MSL-DCC) involved in dosage compensation of the drosophila X-chromosome. Descriptions of the complex's structure and function are given in the papers ...
6
votes
1answer
3k views

What causes the colors we see in eyes?

Genetics aside, what are the biochemical reasons the different colours of human irises? Also, related, how does eye colour change, particularly in childhood? (example: my eyes used to be blue, ...
9
votes
2answers
176 views

Can life subsist on anything else but water?

Inspired by an answer to a previous question. It's hypothesized that extraterrestrial life could be based on silicon rather than carbon as its main structural element. Are there similar theories ...
1
vote
0answers
61 views

When a Lysosome's bonding sites all get bonded to, what is the most resource and energy efficient way to handle the used Lysosome?

Let's say a Lysosome that bonds to a hazardous material to neutralize it has all bonding sites used up. would it be more safe / efficient to excrete the entire Lysosome / hazardous material molecule ...
8
votes
1answer
156 views

Why must a planet be in a star's “habitable zone” to have life?

I have seen many articles that talk about a star's "habitable zone" as the ring around it which is just the right temperature for planets to have liquid water, which is necessary for life. My question ...
9
votes
1answer
670 views

Could humans and chimpanzees hybridize?

In some cases two species can hybridize. For example, Tigers and lions can hybridize to produce "Ligers". Would it also be possible for humans and chimpanzees (or any other species) to hybridize ? ...
5
votes
2answers
600 views

Possibility and feasibility of producing designed antibodies with bacteria

As stated here it is possible to produce fragments of antibodies in bacteria and harvest them (from the medium, I guess, but I don't have access to the full article). As it is possible to design ...
4
votes
1answer
53 views

How different are tissue-specific fibroblasts from each other?

I am planning to utilize a new system in our lab, in which I will co-culture cancer cells from different tissues with fibroblasts. I have the option to receive skin-derived primary fibroblasts. I ...
3
votes
0answers
204 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
7
votes
1answer
362 views

What is the name of the appendage that mantis shrimp use to rub their eyes?

In this short clip, you can see the mantis shrimp using some sort of feathery arm/claw to rub its eyes. Looking at a diagram here, it appears possibly that it's using maxillipeds. Someone with ...
1
vote
1answer
126 views

Single Nucleotide Polymorphisms and diseases

I am writing a report on how Single Nucleotide Polymorphisms occurring in each of these regions: Transcription factor (TF) binding sites Epigentic signals Splicing variants MicroRNA binding sites ...

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