6
votes
1answer
121 views

Ideal low-protein binding membranes

I'm looking through the literature on the topic. It seems like hydrophilic PVDF membranes are ideal for low-protein binding but it also sounds like Regenerated Cellulose is an appropriate ...
8
votes
1answer
102 views

Is bootstrapping an acceptable way to determine standard error for binding constants?

I'm measuring binding constants my system and I appreciate the usual methods of using replicates to measure standard errors and using those errors to calculate propagation of error. I'm curious if ...
7
votes
1answer
889 views

How does one calculate the resolution of a crystal structure?

Considering that I have a diffraction pattern of a crystal, how would I calculate the resolution of a structure in Angstroms? How does that value differ from the information that I would get from the ...
8
votes
1answer
746 views

How strong is spider silk?

Spider silk is pretty darn strong and all sorts of comparisons are made to steel. I'm more curious about the various moduli of spider silk and how it compares to other materials. What is the Young's ...
5
votes
1answer
229 views

Stabbing muscles when flexed and when relaxed

Just a random question, would a sharp object pierce your muscle more(stabbed with the same amount of force) when the muscle is flexed or when relaxed?
8
votes
1answer
116 views

How would a warm winter affect maple sap production?

The past winter (2011-2012) was warmer than usual. Trees are normally tapped in late February when the daily maximum temperature goes above freezing. However, assuming that there have been ...
15
votes
1answer
350 views

What is the lowest pressure at which plants can survive?

What is the lowest pressure at which plants can survive? How the plants behave in a Martian-type atmosphere? Is there any plant that can survive such atmosphere? Can a lichen grow at Martian ...
12
votes
5answers
4k views

Can any protein be phosphorylated?

I am working with an Arabidopsis mutant with an F-box protein knocked out. It has been shown that F-box proteins targets must first be phosphorylated (Skowrya et al., 1997). I have heard of ...
6
votes
2answers
199 views

PCR amplification and error propagation

Say I have one dsDNA that undergoes normal PCR (where amplification is exponential). If there is a mistake, say a G is swapped for an A during the 2nd round of replication, what percentage of the ...
4
votes
1answer
191 views

What is the molecular mechanism of cystine bond formation?

My bioinorganic and redox chemistry isn't up to par and I'm having a very difficult time understanding the chemical mechanism behind cystine bond formation. Most reactive environments are actually ...
10
votes
3answers
2k views

How long can E. coli stocks be stored at -20°C?

I'm volunteering for a biohacker lab - biocurious in Sunnyvale. The have a pretty good set of equipment - gel boxes, incubators, but they don't have a -80°C freezer yet. I'd like to set up some ...
3
votes
1answer
491 views

Displaying nucleotide at a single position from RNA-seq reads in a BAM file

How do I display a single nucleotide position from reads in a BAM file? I have been looking at variation using samtools mpileup, but I want to actually just display the nucleotide at the position I am ...
9
votes
4answers
3k views

What is the closest species to humans in animal kingdom?

I presumed chimpanzees were the closest relatives of us. However, after watching this TED Talk, it seems bonobos are closer to us both in skeleton and behavioral similarity than chimpanzees. I once ...
6
votes
1answer
158 views

Which cryogenic vials and caps are ideal for storing glycerol stocks?

There are internally threaded, externally threaded, natural caps. For the vials, there are skirts, star-footed, and various bottoms. Why do any of these things matter?
4
votes
3answers
104 views

How to create a collection of anonymous sequences for teaching and testing? [closed]

I am looking for a large collection (>1000) of sequence files (eg. FASTA) from any real organism or a tool to create such a collection. The sequence files would be used for teaching and for testing ...
3
votes
1answer
264 views

Does chicken embryo form the disc called “discoblast” in the cleavage and blastulation of chicken?

This thread is related to my previous thread which is still unsolved mainly. I need to be able to compare chick and human cleavage and blastulation of zygote. My friend says that Chicken have ...
3
votes
1answer
924 views

Is secondary follicle or Graafian follicle arrested in the second metaphase of oogenesis?

My note about which I am not sure: Secondary oocyte is arrested in the metaphase II monthly after puberty until fertilization with sperm. The following picture also satisfies my sentence: . So ...
8
votes
3answers
305 views

Long-term-potentiation and memory. Where do we stand?

I was reading the answers to the question: How and where, in the human brain, are memories stored? and, as expected, LTP and LTD came out. Every time I read about LTP/LTD there is always something ...
8
votes
1answer
237 views

Is it possible to add a new sequence to a blast database without recreating it from scratch?

I have a new sequence and I want to add it to a pre-constructed BLAST database containing other sequences. The makeblastdb help does not give any hint on this regard.
11
votes
4answers
255 views

What can you tell about a person, having only their whole genome as information?

Well, the question is in the title, no explanation need.
7
votes
1answer
4k views

Why does RNA adopt an A-form helix?

RNA is known to form an A-form helix, while DNA generally forms a B-form helix under physiological conditions. From left to right: A-form DNA, B-form DNA, Z-form DNA. Image created by Richard ...
2
votes
2answers
727 views

What are the most important differences between HSP70 and HSP90?

Question originally asked on Quora. These proteins have many functional similarities, so why do cells need both to handle unfolded proteins?
7
votes
1answer
124 views

PDB Mining: Why Do I Find Atoms Less than 1 Angstrom Apart?

I am attempting to find potential Hydrogen bonds between Hydrogen donors and aromatic ring acceptors. I do this by predicting the location of Hydrogens on residues and then calculating how far these ...
6
votes
1answer
517 views

What forms the human amniotic sac?

I am trying to understand the formation of embryonic disc for human and chick so the following question is related to here about formation of embryonic disc. I had thought that the amniotic sac forms ...
7
votes
3answers
577 views

Determining potential protease sites within a recombinant protein

My expressed proteins are frequently truncated and I'm trying to figure out which bands are which. The first thing to come to mind is using PeptideCutter from ExPASy but there is just a data deluge of ...
7
votes
1answer
157 views

Taxonomy for plasmids

I liked the question about F' and R plasmids. What is a good resource that describes what is known about the functions and diversity of plasmids in the wild? Prokaryotes or eukaryotes? Wikipedia ...
6
votes
2answers
815 views

Density of neurons/cells in the mouse brain

Could someone point out some relevant papers or resources (an online DB maybe?) describing the density of neurons (or, more in general, of cells) in different areas of the mouse brain?
8
votes
1answer
216 views

What are the biochemical processes occurring when food spoils?

Let's assume for a minute that microbes themselves and their direct toxic products (i.e. endotoxins) aren't toxic to humans. Let's also discount any innate immune responses the body mounts against the ...
12
votes
3answers
689 views

Why do restriction enzymes tend to have an even number of bases in their recognition site?

When reading my textbook I noticed that in all examples but one from eight the recognition site was an even number of bases. I wondered if this was just a co-incidence, so I took the data from this ...
16
votes
1answer
639 views

ATP cost for gene expression

How would you estimate the number of ATPs required to transcribe, export and translate a single eukariotic protein?
5
votes
3answers
143 views

Can genetically modified genes jump to bacteria in the eater's intestine?

The Guardian ran an article a while back talking about GM gene's jumping to bacteria in an eater's intestine. Has other research confirmed this phenomenon?
12
votes
2answers
458 views

Which is more important for protein expression mRNA structure or codon optimization?

The field seems extremely divided on the debate. On one hand, artificial experiments have suggested that synonymous mutations don't correlate with gene expression but rather, the mRNA 5' structure is ...
5
votes
3answers
1k views

Fetal development, gastrulation and embryonic disc

I am completely confused by the images circulating on the internet of human gastrulation. First, lets see how it happens in deuterostomes. This image depicts the process: (image is from Wikipedia) ...
4
votes
1answer
243 views

What did pangolin scales evolve from?

Were they developed anew or a heritage from fish?
10
votes
2answers
437 views

Antibacterial hand soaps (and related products); what are they good for?

From Wikipedia: Household use of antibacterials in soaps and other products, although not clearly contributing to resistance, is also discouraged (as not being effective at infection control). ...
6
votes
2answers
100 views

Is there a term for tool use in animals?

Is there a technical/scientific term that scientists use to refer to tool making abilities found in certain types of animals? Reference ...
17
votes
3answers
225 views

How crowded is the bacterial cell?

I was wondering what is the protein concentration in an E. coli cell. When studying enzyme kinetics and activity in vitro, I would argue that the substrate and enzyme concentrations resemble those in ...
6
votes
2answers
2k views

Can any other animal choke on food?

I read somewhere (I think it was Bill Bryson's book on the origins of the English language) that of all animals, we are the only ones that can choke on food (having something to do with how our larynx ...
3
votes
0answers
34 views

Can a cell culture insert plate be used with a vacuum manifold?

There exists 96 well plate setups for use with vacuum manifolds. Is it possible to use 6/12/24 plate inserts with the same setup?
7
votes
2answers
26 views

What data / tools exist for mapping of disease trends?

I was just looking at the Google Flu Trends map. Google Insight could be used to gain similar information for other trends in disease keyword searches. I started wondering how valid are keyword ...
8
votes
2answers
158 views

Are prions an important driver in evolution?

I understand that prions have been implicated in the passing on of epigentic information[1]. Are prions thought to play a significant role in the evolution of organisms? Alberti S, Halfmann R, King ...
13
votes
1answer
237 views

Do the majority of fish lack scales on their heads?

Based on pictures, it seems to me that a vast majority of fish species that have scales do NOT have scales on their heads. Is that fact true? To make this properly answerable: lets' define a ...
12
votes
4answers
399 views

Questions to ask to a panel of people that will be sequenced

Some genes have been shown to be associated with left-handedness. Working with some clinicians, I've recommend them to ask their patients (whose genome will be sequenced) if they are "right or ...
5
votes
1answer
718 views

Do eye muscles relax when you close your eyes?

I wonder whether the eyes muscle relax when you close them. Common knowledge told us the eyes muscle get tense when you stare at nearby object. If you close your eyes, you eye lids have 0 distance ...
5
votes
2answers
4k views

Why is it that cats can jump so high for their size, compared with humans?

My cat is about 1' high at the shoulder, and I am a little over 6', but my cat can easily jump onto something as high as I am. That is 6x it's height. If a cat can do this, then Why can't I jump up ...
9
votes
2answers
2k views

If I graft two trees together while young, will they grow as one plant?

If I were to graft two apple saplings together, by bending the tops toward each other and lashing them together, will the plants grow as one and benefit from one another, or will they be fighting each ...
3
votes
1answer
2k views

Why did the sensitive plant (Mimosa pudica) evolve its leaf-closing mechanism?

Why did the sensitive plant, Mimosa pudica, evolve its leaf closing mechanism? Does it heip in a heavy storm? Does it scare off whatever animals might think it a good meal?
19
votes
1answer
249 views

Evolutionary origin and exogenous cues of ~28 day infradian rhythm?

The most obvious example of an approximately monthly biological cycle is the human menstrual cycle. My questions are the following: Is it known when and where this cycle or one like it arose? What ...
10
votes
2answers
173 views

Can DNA produce ribozyme-like structures?

RNA is known to act as an enzyme via its ability to fold itself in specific ways. Is DNA capable of such structures? Or is it some biochemical reason stopping the folding? Have they been observed in ...
7
votes
1answer
220 views

How do plants 'tell time' for circadian rhythms based on a ~24 cycle?

I've read that many plants have some sort of circadian rhythm where they perform a certain action on a cycle of about 24 hours, like the mimosa plant opening and closing its leaves. Obviously this is ...

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