2
votes
0answers
156 views

Can protein sample be made 2% Triton X-100 free?

The protein I am purifying needed an elution buffer with 2% Triton X-100. I formulated the elution buffer not keeping the CMC in mind. My goal is to make my protein sample triton free to check its ...
0
votes
1answer
75 views

How much mass does a person lose in a day through breathing and perspiration?

Water and carbon are lost through perspiration and breathing. How much mass is lost in this way in a single day for a typical person?
3
votes
0answers
44 views

What has to duplicate before cell enters M phase? [closed]

What has to duplicate before cell enters M phase? DNA histones centrosome mitochondria (any other organelles?) condensins (not sure?) have I forgotten anything? Thank you in advance!
2
votes
1answer
308 views

How can a brainless Cnidarian hunt and defend itself?

Cnidaria, such as jelly fish and sea anemones are radial symmetric en feature a simple body plan. One notable aspect is the lack of a brain. Hence, there is no central guiding structure, no mind or ...
2
votes
1answer
290 views

Does increased cell turnover cause cancerous mutations?

If a certain set of cells or tissue are undergoing a lot of reproduction and repair cycles for some reason, does this inevitably lead to cancerous growths? If the mutation rate exceeds the normal ...
3
votes
0answers
45 views

What are the biological mechanisms of candy causing nausea?

As I sit here after eating too much chocolate, I wonder: What are the biological mechanisms behind eating too much candy candy causing nausea in a healthy individual? Is it a spike in blood sugar, ...
2
votes
3answers
564 views

True or false & fill in the blanks

A ribosome is where amino acids are linked together by peptide bonds. A virus is NOT considered prokaryotic because it does not have a membrane. The nucleotide ATP is one of the most used energy ...
2
votes
1answer
44 views

Is it possible to estimate how much variation in common phenotypes is predicted by genetics?

Phenotypes such as; skin color, eye color, nose shape, lip size, limb proportions, and skull shape. Im trying to find a source for this quote. "If you ask what percentage of your genes is reflected ...
3
votes
2answers
288 views

Assessing probability from a pedigree

This isn't exactly homework but I'd like to treat it as such. I'm asked to find the probability that both (III: 2) and (III: 3) are carriers given that (II: 7) and (III: 1) exhibited the recessive ...
1
vote
1answer
236 views

What is Mendelian Segregation and how is it related to the Hardy-Weinberg equilibrium?

I can't find the exact definition of Mendelian segregation. In addition, I am wondering how it is related to the Hardy-Weinberg equilibrium?
2
votes
0answers
59 views

What species of Cerapoda lived during the Jurassic?

So far, I've found eleven species of Cerapoda that lived during the Jurassic period. Are there any other known species that I've missed? The eleven that I've found out about are: Agilisaurus ...
2
votes
1answer
232 views

How do you convert mtDNA sequences in FASTA to FSTAT format?

I've got control region sequence data from a population of shark and I'm looking to convert this from FASTA to FSTAT in order to calculated the effective population size of females. The software I ...
2
votes
1answer
35 views

Are there any generic (not dependent on symptoms) ways to find parasite infections?

Are there any generic ways to determine if a person is infected with a parasite? With "generic ways" I mean ways that do not rely on symptoms caused by a particular parasite. Also I am mainly ...
0
votes
1answer
113 views

Which of these components has C-H and COOH groups [closed]

A) Amino acids and triglycerides that have a carbon-carbon double bond or B) proteins and fatty acids I think they ...
5
votes
1answer
134 views

How to measure metabolic rates of microscopically small animals?

I want to measure metabolism rates in Tardigrades Metabolic rates will be assessed immediately following their exit from cryptobiosis (an animal dormant state). I know that oxygen levels is the ...
33
votes
3answers
8k views

Can humans perceive sounds above 20 kHz?

In the never-ending debate raging in the audiophile community about sound quality and what humans can or cannot hear, it is very very very very incredibly often cited that the upper-limit of the ...
0
votes
1answer
76 views

Almost philosophical question about controlling a pest/plague

Once in a math congress someone presented a paper on a mathematical model used to predict the impact of certain measures that could be taken in order to control some pest/plague. A guy in the audience ...
3
votes
3answers
315 views

How do we look inside the cell?

My sister is in 9th grade biology and her teacher avoided answering the question of how we actually study the inside of a cell. I haven't taken biology in a while but I'd like to give her an answer. ...
1
vote
1answer
504 views

How to determine if this blood disorder is recessive or dominant?

This question is from "Concepts of Genetics," Klug & Cummings, 10e. "Thalassemia is an inherited anemic disorder in humans. Affected individuals exhibit either a minor anemia or a major anemia. ...
1
vote
1answer
204 views

Pituitary giants - is the fusing of growth plates dependent on amount of growth hormone in blood?

I wanted to ask a couple questions related to pituitary giants (people who are giants because of some anomaly, such as a tumor, in their pituitary gland). Some of these giants seem to keep growing ...
1
vote
1answer
89 views

What is the biological basis for tiredness after eating? [duplicate]

I can sometimes feel quite drowsy after some "meals" (e.g. a cold cut & cheese sandwich with juice on the side) and less drowsy (or maybe less often) after others (such as a salad). This ...
3
votes
0answers
225 views

How to prevent e coli from clumping (for FACS)?

I'm performing FACS on e coli, but the cells are clumping together so each event is multiple cells. I ran a control where I had one flask of e coli expressing GFP, and one flask expressing RFP. Run ...
1
vote
2answers
44 views

Why don't stem cell therapies use viruses?

Why don't stem cell therapies use a virus to deliver gene editing sequences to stem cells instead of the harvesting-transformation-transplant route. I thought it might be because of a lack of ...
5
votes
3answers
1k views

How does vasoconstriction/vasodilation change blood pressure?

Background: I am coming at this question from an electrical engineering background, and I feel like I am missing certain assumptions that are going into the statement found in my physiology textbook, ...
7
votes
1answer
114 views

What were the first neural systems like?

I'm curious about the origin of the neural network. I'm thinking perhaps once life evolved beyond the single cell organism, it needed a simple neural network to coordinate those cells, and cell ...
1
vote
0answers
104 views

Factors for sensitivity to electric shock

I'm frequently getting electric shocks in my office when touching things. I basically know why this happens and how to avoid it. Still I started to wonder what would be the best way to touch ...
2
votes
0answers
48 views

Death Psychology [closed]

Why do we sympathize in general when we see something or someone dying even if we personally don't know it/them? Is there any species which sympathize with the death of its own species? Is there any ...
0
votes
1answer
138 views

What shows how healthy someone is by their heart rate? [closed]

What shows how healthy someone is by their heart rate - how low it is at resting pulse or how low it is during exercise or how large the difference is between the resting rate and rate with load? Any ...
4
votes
1answer
306 views

If we change our breathing rate so easily, then what is the point of measuring it?

When we are self-conscious of our own breathing, its rate changes very easily. Therefore, if it changes so easily, what is the point in measuring it? Thank you for your time and effort
2
votes
2answers
2k views

Cell evolution: immortality vs reproduction

Many sci fi movies produce interesting ideas and technologies that we seem to be able to realize in real life at some point. "Lucy" was not one of those movies. But Morgan Freeman's speech in the ...
2
votes
1answer
94 views

What are the advantages of blood's redness? [closed]

Although this page (http://evolution.berkeley.edu/evosite/evo101/IIIE5aNotadaptation.shtml) denounces the idea that blood's redness is not an adaptation, I remain inquisitive on the matter since no ...
9
votes
1answer
3k views

why is DNA antiparallel? Can it be parallel?

My biology textbook mentions that DNA is antiparallel and it got me wondering... Can DNA be parallel? What would happen if it was parallel? could DNA still replicate right?
2
votes
1answer
89 views

Glycerol 3-phosphate nomenclature

Why is Glycerol 3-phosphate named in such way? Shouldn't it be named as Glycerol 1-phosphate by proper IUPAC- nomenclature?
1
vote
1answer
94 views

Which came first in hominid evolution: language or bipedalism? [closed]

I suspect both language-use and upright-posture have anatomical consequences. So does the fossil record show which came first? NOTE: To the folks who marked this question "homework": This is ...
3
votes
0answers
48 views

Why is ATP the main nucleoside triphosphate used to exchange energy? [duplicate]

Out of all of the nucleoside triphosphates what makes ATP the most used? Is it its structure? The amount of energy it contains? Why is GTP not used as much? What is the deal with the other nucleoside ...
2
votes
2answers
153 views

Parallels between pixelized image and the human retina

On Wikipedia it is explained that the human eye has a certain 'resolution'. Does this mean that the retinal image consists of pixels? If yes, what shape would these pixels have?
2
votes
1answer
91 views

In a typical population, what might the percentage of plants be to predators and everything inbetween?

I'm not sure if this will be a simple question that is almost a natural law for an ecologist, or a complex serious of assumptions. I vaguely remember from school that only 10% of the energy is ...
0
votes
0answers
83 views

Known ways to modify the genetic structure of the 13 loci used in CODIS

After reading the answer to Does our DNA change during our lives?, I was wondering if and how it would be possible to change the structure of the 13 loci that are used in the CODIS database, in such a ...
23
votes
1answer
7k views

Why do adults lose hearing at high frequencies?

Children and teens can hear high pitched sounds that adults can't hear anymore. Why do adults lose high-frequency hearing?
0
votes
2answers
1k views

Why is water a polar molecule? [closed]

Also, what is the definition of polar in this case? (9th Grade) Does it have to do with the bonds it makes?
1
vote
1answer
108 views

Multi-modal distribution for gene expression data

Why would some genes have more than two modes in their expression distribution? What external factors would cause this anomaly? I'm referring to the expression distribution of a gene across different ...
3
votes
1answer
233 views

how do macrophages have the capacity to digest pathogen in opsonization

how do macrophages have the capacity to digest pathogen in opsonization but not in the first time when the pathogen is new and they play the role of antigen presenting cells(APCs) first ...
7
votes
1answer
159 views

What is the mechanism by which lamins regulate gene expression?

The heterochromatin is generally localized at the nuclear periphery (also near nuclear lamina), whereas active genes are preferentially found in the nuclear interior. Children with ...
32
votes
3answers
22k views

Why draw blood from veins rather than arteries?

Why draw blood from veins rather than arteries? Is it more convenient or safer?
2
votes
1answer
58 views

How semi-synthetic cell with artificial DNA works

I know that by now this is old new but I heard that a cell that used a synthetic pair of nucleotides, called X and Y, have been made. My question was, how did the cell understand the X and Y ...
5
votes
3answers
1k views

How many different kinds of polypeptides, each composed of 12 amino acids, could be synthesized using the 20 common amino acids?

How many different kinds of polypeptides, each composed of 12 amino acids, could be synthesized using the 20 common amino acids? The book's answer is $20^{12}$. However, I disagree. This result ...
2
votes
2answers
261 views

Why asexual reproduction?

When I took a course on genetics and evolution, I learned that recombination and sexual reproduction is advantageous compared to asexual reproduction. Sexual reproduction allows more combinations of ...
1
vote
2answers
232 views

How does DNA damage cause ageing in yeast?

As I understand it, in yeast ageing there is daughter cell and mother cell. The daughter cell is has newly "fresh" DNA and mother cell dies after some counts of replication. What happens to the ...
3
votes
2answers
107 views

Energy metabolism in Cancer cells

The TCA cycle intermediate Isocitrate dehydrogenase commonly undergoes point mutations in cancers. This allows IDH to reduce a-Ketogluterate to 2Hydoxygluterate, causing a reduction in pVHLs ability ...
3
votes
1answer
408 views

What’s the reason for isovolumic contraction and isovolumic relaxation?

During cardiac cycle, there are two periods in which the heart volume doesn’t change, but there is a change in tension/pressure. It takes about 0.25-0.35 second to achieve this change. I searched in ...

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