5
votes
1answer
304 views

Western blotting with multiple antibodies

Normally I wash/detect with one primary/secondary-HRP antibody pair, strip, then wash/detect with the other primary/secondary-HRP pair which works well. However, I recently started working with a ...
2
votes
1answer
88 views

Anatomy of nervous system's sensory pathways

When I touch my hand on a hot stove, I feel pain. I'm interested in knowing all the main "endpoints" (components/parts of the body) that are involved in relaying this pain signal. As I understand it ...
1
vote
1answer
69 views

How and where do nerves share pathways to the brain?

I am interested in understanding how pain receptors send signals to the somatosensory cortex (the part of the brain that registers various nerve signals such as pain, presure, temperatures, etc). ...
1
vote
1answer
8k views

Why do the major and minor groove exist in DNA?

So I know that the major/minor groove arise from the offset pairing of the two strands in the helix. All my textbook tells me is that "pitch causes the off-set pairing of DNA and this gives rise to ...
0
votes
1answer
58 views

What does 'pedigree information' mean?

This paper uses Pied Flycatcher as a study species. For the population of Pied Flycatchers they studied, 'pedigree information' was available. What exactly does this 'pedigree information' mean?
10
votes
3answers
1k views

Do snakes get dizzy?

I've seen snakes get captured in sacks and I've wondered if the snake would get dizzy if the sack were twirled around. I have not done any tests (and don't plan to) because snakes scare me.
1
vote
0answers
30 views

Would blue light be effective to help average people when sunsets come much earlier in the day?

I know people with SAD (Seasonal Affective Disorder) benefit from blue light in the winter as night-time comes much earlier (in the UK, sunset is around 4pm in December, compared to 9pm in June). ...
2
votes
1answer
119 views

Do distinctions between dry, tickly and chesty coughs have any medical basis?

Background At least in Britain you normally come across distinct kinds of cough medicine "chesty", "dry" and "tickly". Questions Are "chesty", "dry" and "tickly" coughs always due to ...
0
votes
1answer
79 views

Would humans eventually evolve to fight off fatal diseases if we didn't treat/vaccinate for them? [duplicate]

I am definitely pro vaccination, and the question is a bit morbid because people would die, but assuming that we didn't treat or vaccinate people for fatal diseases such as Ebola or the black death, ...
1
vote
1answer
122 views

What specific sensory nerves act as receptors for “pins and needles” (neuropathy)?

According to this excellent answer, the difference between "pain" and "pins and needles" (neuropathy) is that different receptors (sensory nerves) trigger in reaction to different stimuli. Different ...
1
vote
1answer
57 views

STRING ID - allelic differences and splice variants

STRING has protein (amino acid sequences) mapped to a single gene ID. I have these doubts with respect to STRING ID. I find that 1.All alleles of a single gene share the same STRING ID and ...
6
votes
2answers
156 views

Balanced vs Maximized Codon Optimization

I have worked with the optimization of several viral proteins, from different viruses/families, with different results. When Menzella, 2011 was published I tried that method. I found quite the ...
9
votes
1answer
105 views

Statistical method for characterizing the relationship between body mass and metabolic rate

There is a dataset that contains body mass ($x$) and metabolic rate ($y$) from many different organisms. It is common to fit the data to the model of the form $y=ax^b$ and estimate the parameters $a$ ...
4
votes
1answer
208 views

How could be the “Eurasian magpie” bird won the “Mirror Test” where Gorillas failed

EURASIAN MAGPIE VS GORILLA VS How could be the "Eurasian magpie" bird won the "Mirror Test" where Gorillas failed ? In 1982, mirror tests on two, zoo-reared gorillas failed to demonstrate ...
7
votes
2answers
210 views

Use cases of RNA secondary structure prediction

I'm an undergraduate currently writing a thesis on RNA secondary structure prediction, specifically building upon analysis done by the RNAStructure and Unafold software packages by modifying certain ...
1
vote
2answers
56 views

Why not use non-visual self-recognition to test for consciousness in an organism?

Background A well-known test for consciousness in an organism is to observe its ability to recognise itself in a mirror (see wikipedia article: "Mirror test"). Question Why haven't I come across a ...
5
votes
2answers
185 views

What is Environmental Robustness? Is it different from plasticity?

Hansen (2006) in his review uses the concept of environmental robustness independently of the other concepts of robustness (at pages 139 and 140) without defining ...
2
votes
0answers
43 views

Native vs Immigrant plants [closed]

Is this statement applicable to any known plant/region combinations? Plant species X, Y, and Z are truly indigenous to region R. Other "immigrant" species can be destroyed by drought or harsh ...
6
votes
2answers
3k views

Why does anxiety cause diarrhea or constipation?

Anxiety sometimes cause diarrhea, sometimes constipation, and sometimes both. It's interesting because it seems their underlying neurophysiology is somehow different. What are underlying physiological ...
4
votes
1answer
172 views

Circulating factors affecting human health/longevity

Circulating factors present in young mice have been shown to promote rejuvenation of aged mice, suggesting that tissues have inherent capabilities to regenerate, and circulating factors may be ...
2
votes
1answer
368 views

Speciation of humans? [duplicate]

Since we homo sapiens (and all other species) are continuously evolving, are humans undergoing speciation based on our environment? OR has there been any difference across people that could say ...
35
votes
2answers
2k views

Can brain cells move?

I was discussing this with my brother. I'm pretty sure I read somewhere that they can move. Thanks EDIT: By movement I mean long distance migration (preferably within the brain only).
4
votes
1answer
302 views

Do plants have preference for the form of nitrogen as nutrient?

In the nitrogen cycle (ecology), it is usually described that plants can use nitrogen in the form of ammonium (NH4+) and nitrate (NO3-). Do plants prefer one form of nitrogen over the other?
0
votes
1answer
28 views

Relation between trigeminal and facial nerve in sympathetic response

In my neuro-anatomy course notes I found that the facial nerve "uses" the trigeminal nerve to reach its targets. However, I have yet to find any diagram or reference that indicates how this is done. ...
8
votes
1answer
118 views

How to validate the regulatory interactions inferred from gene expression data?

My algorithm learns regulatory interaction between genes using Bayesian Network approach from gene expression data. After the algorithm has converged to a network of interacting genes, how to validate ...
2
votes
1answer
55 views

Can plants directly use sulfuric acid?

In the explanation of the sulfur cycle, it is often said that sulfur moves from the atmosphere to the ground by acid rain in the form of sulfuric acid. Can plants directly use sulfuric acid to ...
7
votes
1answer
196 views

Why do we still have genetic disorders that show anticipation?

Anticipation whereby symptoms of genetic disorders occur earlier and with more severity as it is passed from one generation to the next. So wouldn't these inherited diseases over a few hundred ...
3
votes
1answer
144 views

Why does weight loss lower blood pressure?

I have read that weight loss is an effective lifestyle modification for people with hypertension, and systolic pressure may be reduced by up to 1-2 mmHg for every 1kg of weight lost. What is the ...
6
votes
2answers
486 views

Skin porosity in glans penis when erect

(Apologies if this is a too weird/inappropriate question in this forum. I only thought that I ask it since it happens to be part of human life.) The skin on a human typically blocks many substances ...
4
votes
1answer
124 views

Does DNA analysis allow determining amount of chromosomes?

Nowadays it is possible to sequence the DNA of extinct species, such as the Neanderthals, the Denisovans, and others. Is it possible to determine, solely from the sequenced DNA or from known bone ...
3
votes
1answer
50 views

Estimating RPM to RCF in Methods from Older Papers

I'm attempting to replicate a cell biology method from a 1958 Laboratory Investigation paper. The protocol is for the isolation of an extracellular matrix protein, and a key step is a centrifugation ...
4
votes
1answer
2k views

Bug Identification: Is this small tubular, black bug a bed bug?

So I found this tiny bug on my wall in the bedroom of my apartment. I estimate it is about 3mm long by 1mm wide. Is it a bed bug? It does not seem to match any of the pictures I've seen online. ...
0
votes
0answers
35 views

Maximum heart rate increase/decrease while physically active [duplicate]

I'm a computer scientist and implementing a heart-rate monitor for physical exercise. While I try to improve my code, I stumbled over the question how fast the heart rate can change within a given ...
8
votes
1answer
219 views

How are bones growing, if bones are not connected to the brain?

If the bones are not connected to the brain, how is their growth controlled? This question is not a duplicate of the question Mechanisms of bone growth, as this question deals with how bone growth is ...
3
votes
1answer
677 views

Why do small fonts strain the eyes?

I understand that too much close reading will strain the ciliary muscles of the eyes. But what about small fonts? The contraction of the ciliary muscles is dependent on the distance not font size. In ...
5
votes
3answers
347 views

Studying effects of alcohol on cells

I am wondering about the logistics of a simple experiment with say 3 types of alcohols, and various concentrations of each, with a control(s) I would like to research the effects of alcohol on ...
4
votes
1answer
216 views

Why are most transcription factors enhancing the expression rather than repressing?

One can classify the effects of Transcription Factors (TF) on gene expression into two types: it either enhance or repress the gene expression. I have always been told that most of transcription ...
0
votes
0answers
51 views

How can site-directed mutagenesis be used to suppress the production of anti-antibodies?

In a previous post of mine, I asked how to supress the creation of anti-antibodies in vivo. In the answer, it was mentioned that site-directed mutagenesis could be used. Currently, I can't find mcuh ...
3
votes
1answer
77 views

Well to well variation in thermal cycler fluorescence?

We have an old BioRad ICycler Thermal Cycler with MyIQ single color fluorescent detector. While it's meant for RT-PCR, we've been using it for melt curves and binding assays for different types of RNA ...
7
votes
1answer
163 views

Number of transcription factor genes in the human genome

What is the number of the transcription factor genes present in the human genome? Does this value differ compared to Mus musculus, Drosophila melanogaster, Arabidopsis thaliana, C. elegans and S. ...
3
votes
1answer
169 views

How to attract spiders to my house?

I've been living in my apartment for a year now, and noticed something lately. During spring and summer I have quite a lot of midges indoors, and even now there are some. But there are no spiders! So ...
2
votes
0answers
20 views

Resource for finding Repressive/Inhibitory factors for a given gene?

I have a list of genes for each of which I'd like to find: A list of transcription factors that up-regulate the gene A list of inhibitory factors that down-regulate it. I used this tool on ...
0
votes
1answer
48 views

Effect of Western chicken and GH [closed]

I heard this clause from my teacher one week ago: Chicken are grown in half a year until eaten in Baltic countries - Estonia, Latvia and Lithuania, for instance, [probably in other parts of ...
0
votes
1answer
3k views

Can swallowed fingermails, hair, or skin get caught in your appendix? [closed]

There is a common saying in my place: If you eat your skin, hair or nails, it will be deposited in the cecal (Vermiform) appendix, and can cause appendicitis. (This is mostly told to children to ...
14
votes
4answers
12k views

Why deoxyribose for DNA and ribose for RNA?

Why is DNA made out of deoxyribose and RNA made of ribose? Why can't they both use ribose or deoxyribose? I think that the deoxyribose gives an advantage in storing genes, the job of DNA and ribose is ...
11
votes
1answer
3k views

Why don't McDonald's fries decompose?

So I was cleaning out my car and found a McDonalds French fry. as I don't eat anything in my car I know exactly who and when this fry is a result of. The when is + 10 months old and it could pass off ...
6
votes
1answer
857 views

Why does DNA have its name?

Why is DNA called deoxyribonucleic acid and not something else? I get the nucleic acid part (because that's what DNA is made of) but what about the deoxyribo- part, especially the ribo- part. Maybe ...
1
vote
0answers
28 views

Would biotechnology in agriculture actually decrease biodiversity, as many people say? Does it have to?

I've heard the claim that because farmers would be using the same seed, you would end up with an entire crop population that could be susceptible to a disease. Like if you had a diverse population of ...
2
votes
3answers
196 views

fastq-dump using mkfifo to stream data

So, fastq-dump has the ability to be run on just an SRA file accession number, such that the SRA is converted to FASTQ on-the-fly, and the SRA doesn't have to be written to disk. I'm curious whether ...
3
votes
1answer
72 views

Western blot alternatives

Given an in vivo sample from an experimental infection, I would like to see if a bacterial protein is present. I've been thinking about Western Blot using sera from animals infected with the ...

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