5
votes
1answer
99 views

Can you “spam” bird sounds so that birds no longer interpret them right?

Is it possible to make birds confused by repeating recorded chirping or singing? Or do they always perceive them as usual, even if the situation is clearly not as described by those sounds? For ...
1
vote
1answer
65 views

Clarification of the procedures to amplify cDNA needed

The typical procedure to obtain cDNA from genomic extracted DNA is the following: RNA is extracted from desired tissue, RNA/DNA hybrid is obtained by virtue of reverse transcriptase RNA dependent DNA ...
12
votes
1answer
3k views

Do we actually get more sick (flu/cold) during winter?

The word flu derives from the Italian phrase "influenza de freddo" meaning "influence of the cold". Indeed it is that time of the year when my colleagues seem to have the flu/cold more often than ...
2
votes
1answer
95 views

Could someone clearly define what a gene variant is with an example

Could someone clearly define what a gene variant is with an example. I am totally confusing myself with SNPs, alleles, variants and how the major and minor allele are defined. A clear example with ...
3
votes
1answer
82 views

Chemoautotrophy in large organisms?

The major nutritional mechanisms include chemoautotrophy, heterotrophy (including parasitism and saprotrophy) and photoautotrophy. All of these modes of nutrition developed first in prokaryotic ...
3
votes
0answers
388 views

How far can ants travel from their nest? [closed]

I live in a top floor apartment and we are being invaded by black garden ants. I can only follow their trail outside so far and have no idea where their nest is. How far do ants travel from their ...
4
votes
2answers
227 views

Do animals suffer from “Human” mental disorders?

It is tragic, but apparently Killer whales and Dolphins can commit suicide too (e.g. here)... This suggests they can become depressed. I wondered whether they were "clinically" depressed like many ...
4
votes
1answer
266 views

Body size, what are the evolutionary trade-offs?

Background Considering just the "Kingdom Animalia" branch of organisms. It is clear that bigger does not necessarily mean better - there is large variation in body size... From the 94 µm long ...
4
votes
3answers
205 views

Why do we need deep sequencing?

Why do we need deep sequencing? Why cannot the sequencing technologies read all the nucleotides correctly at the first read? Sorry since this question is too trivial, I don't have a biological ...
1
vote
1answer
58 views

The reason why researchers usually use cell lines from “blast cells”?

What's the reason why researchers usually use cell lines from "blast cells" (so, immature, like lymphoblastoid cells) for measuring gene expression data? Is that they are growing up, which would make ...
1
vote
2answers
349 views

Blast a sequence against multiple databases

I would like to BLASTP a list of protein sequences in fasta format against multiple protein databases. Since I'm only interested in the first hit of each database, it is possible that BLAST result ...
0
votes
1answer
22 views

How to get conservation of vaccine candidates between hundreds of bacterial strains?

We have identified vaccine candidates via an ex-vivo RNA-seq approach. Next step would be to perform conservation of these candidates (about 20) between multiple bacterial strains (about 200). I would ...
4
votes
3answers
265 views

Modern immunology textbooks

I am looking for recommendations to good textbooks introducing modern immunology. Review articles, preferably modern (post 2000) would also be useful. Please, include a brief comment with each book ...
1
vote
1answer
310 views

What are the differences between HPRD and BIOGRID databases?

What are the differences between HPRD and BIOGRID protein-protein interactions databases? What are their purposes? Why do we need two different databases? How is data collected into each one? How ...
1
vote
1answer
124 views

How is deviated nasal septum related to cold

I have a deviated nasal septum and I always suffer from common cold I was just wondering how a deviated nasal septum can be the cause for chronic common cold
3
votes
1answer
364 views

Is there a maximum pain threshold?

Is there a maximum threshold of pain a Human can experience, beyond which point there is no noticeable difference? I ask this question in part to better understand the definition of pain and its ...
5
votes
1answer
99 views

Could murder be modeled as an infectious disease?

Background "When swine flu hit the population it spiked in certain areas and tapered off in neighboring regions, it hits hardest where people have least protection and this pattern is more ...
3
votes
1answer
66 views

Gradual slowing breath, its effect on health

I've written a computer program which beeps, then beeps after 10 sec, then beeps after 11 sec, then beeps after 12 sec, etc. I tried the following "experiment" on myself: do only one breath between ...
2
votes
1answer
38 views

Calculation of saturation of carbon in surface waters

I've been looking at some journals lately that look at carbon emission from lakes where some lakes act as sources and other act as sinks. The paper that I have been looking at is ...
2
votes
1answer
179 views

Can anyone identify this caterpillar / cocoon?

This insect is about 0.5 mm in length, so it is very small. It doesn't have legs I don't believe, I am not too good at this but I don't know if it's a worm or more like a cocoon for something. I do ...
5
votes
1answer
137 views

By what mechanism can hypoglycemia induce fainting?

Hypoglycemia (Low blood sugar) is said to be among the potential causes of human-faintings. I was wondering by which mechanism this happens. Google search results are abundant; there are lists of ...
4
votes
1answer
151 views

Does conjugation support gene selection?

I have already posted this on chat but haven't got any response. A recent question on group selection stimulated me to ask this here. QUESTIONS: Why should bacteria conjugate? If we consider that a ...
2
votes
1answer
446 views

Mesosome - does it exist?

This wikipedia page says that mesosomes are artifacts produced by chemical fixing techniques. Most of my textbooks still show a mesosome and describes it as having functions like - respiration, ...
1
vote
0answers
28 views

what are hyperbranched amplicons in DNA sequencing?

I am reading an article about single-cell sequencing: http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.2720.html And came across the concept of "hyperbranched amplicons". I googled for it but ...
5
votes
1answer
279 views

Time spent in phases of cell cycle

I am looking for references to papers containing the time intervals spent in different phases of the cell cycle (ej., G0, G1, S, G2, M for eukaryotes) for different cells. In particular, I am ...
1
vote
3answers
219 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
2
votes
3answers
295 views

Bacterial cell lysis buffer used in proteomics procedures

What kinds of detergent-free bacterial lysis buffers exist? The proteins we're extracting will be later analyzed by LC-MS/MS, and we're looking for a lysis buffer that won't interfere with this ...
2
votes
2answers
142 views

Interdependency between organsims on our planet and its relation to ecosystem?

I am not a biologist but I just wonder if all species are interdependent. I mean if the tiger becomes extinct does it affect the ecosystem? If the mosquito or mouse become extinct does it affect the ...
3
votes
1answer
162 views

Empirical evidence for Group Selection?

A controversial concept in Biology "Group Selection", has caused confusion and conflict amongst scientists since the since the mid 1990s. The more general realm of study is termed the "unit of ...
1
vote
0answers
182 views

On the definition of gDCW (gram dry cell weight)

I need some clarification on the concept of gram dry cell weight. The unit acronym gDCW stands for gram dry cell weight. My interpretation is that 1 gDCW is equivalent to 1 gram of dry cells, but I ...
1
vote
1answer
3k views

Is it safe to look at infrared LEDs?

What happens if one looks directly at infrared LEDs? Sometimes I see this kind of LED at night as red dots and I'm also courious about it. I was wondering what would happened if one looked directly ...
4
votes
2answers
229 views

Are these cats calico?

Calico cats are cats that have 3 fur colors and are always female or males two X chromosomes. I've seen many cats that will have 2 fur colors (one of them is usually white) and another, third, color ...
1
vote
2answers
267 views

Is the SRY gene the sole responsible of biological male sex?

It's very well known that the Y chromosome is what determines maleness, but more specifically this seems to happen thanks to the SRY gene located on it. Some individuals have an XX karyotype, but ...
4
votes
2answers
80 views

Question about the expression of MHC

Is it possible to express both the MHC class-1 and MHC class-2 both in one cell?
12
votes
2answers
324 views

Why, specifically, does each generation, on average, improve upon the design of the species rather than degrade it?

In every non-life example I can envision, a copy of a copy is always a degraded or less pure version of the original unless some outside influence acts to correct the copy back toward the ideal ...
4
votes
2answers
89 views

Do all humans experience flatus regardless of their diet?

Flatus frequency is affected by diet, so I was wondering if flatus production solely depends on the food eaten, meaning that some people never ever release flatus because they don't eat any ...
2
votes
1answer
83 views

Determining sequence of oligoribonucleotide

Oligoribonucleotide X was treated with phosphatase (for removal of 3' and 5' - terminal phosphates), then with RNAase T1, which cleaves all phosphodiester bonds located in a 3' position of ...
0
votes
1answer
228 views

What are the universal laws of Nature concerning animals' behaviour? [closed]

I'm looking for very old (centuries) treaty or recent (20 & 21th century) research that state what some of the laws of Nature are, particularly concerning wild animals' behaviour. Such as; every ...
1
vote
0answers
1k views

Bacterial 16S rRNA PCR amplification with universal primers

I am doing an experiment to amplify the 16S rRNA gene from bacteria present in gut contents of fish. After extracting DNA, I perform one-step PCR with universal bacterial primers (27F, 1492R) and I ...
3
votes
1answer
199 views

Do genes play a role in the athleticism and shape of a person?

I am an Indian with a small thin stature with some deposition of fat around my belly.I was wondering if genes play a role in fat deposition in the body for Indian, Chinese, European or African people. ...
4
votes
4answers
299 views

Biodiversity is restricted by genome combinatorics?

Me and some friends are interested in opinions for the following: Conjecture The maximum number of species must be limited by the maximum combinatorial/permutational space that can be occupied ...
4
votes
1answer
119 views

Natural enemies boost deep evolutionary fitness?

"Two species that reduce one another's fitness on microevolutionary (short-term) timescales can increase each other's macroevolutionary (long-term) fitness" Opinions? Intuitive Examples: ...
5
votes
1answer
91 views

What makes alcohol harmful to mammals?

During the alcohol metabolic pathway, harmful by-products are made like acetaldehyde, hydroxyethyl, superox­ide anions, and hydroxyl radicals. How do these toxic compounds harm our tissues? Many of ...
5
votes
1answer
580 views

Could alcohol be used as an antibiotic?

I was thinking about how alcohol is used to sterilize, and I was wondering if it would be possible to use alcohol as an antibiotic? if it kills bacteria, couldn't (conceivably) alcohol in the ...
3
votes
1answer
748 views

Problem with bacterial transformation with electroporation

I have a problem with a bacterial transformation of a yeast gene that I can not solve. I isolated yeast DNA and did a PCR to get my product. I am using pCGCUm vector with a GFP construct. I digest ...
1
vote
2answers
39 views

Regulation of the replication of mtDNA at embryonic level

While reading an article on mitochondrial inheritance I came across this link. The results state that mitochondrial DNA replication is regulated in different cells of an embryo at different levels. ...
1
vote
1answer
312 views

What is the most recent non-primate ancestors of humans?

If we go down the evolutionary tree of humans, (Homo sapiens, Homo erectus, ...), what will be the first non-primate ancestors?
3
votes
1answer
53 views

Are there cannibalistic k-selectors?

My understanding of r- and k-selectors does not go deeper than the wikipedia article. I understand k-selectors to be creatures that put tendentially more effort and energy into rearing their ...
3
votes
2answers
1k views

Are Italians genetically separated from other Europeans?

I was reading this Wikipedia article about the genetic history of Italy, and I found it interesting. There are however a few things that puzzle me, because they seem to contradict each other. For ...
3
votes
1answer
86 views

What's a replicate line?

The methods section inside a paper I'm reading make mention of replicate lines. Example: "We founded 10 replicate lines from a single clone". This is in the context of experimental evolution and ...

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