6
votes
1answer
141 views

Number of spindle fibres during Metaphase?

During metaphase, the chromosomes are arranged on the equatorial plate and are attached to spindle fibres. After S phase, can the cell be said to attain the configuration of 4n? Also, during ...
6
votes
2answers
198 views

Why are some viruses not communicable person-to-person?

Below are two quotes from the CDC about Hantavirus: "Researchers believe that people may be able to get the virus if they touch something that has been contaminated with rodent urine, ...
6
votes
1answer
384 views

Photosynthetic Pigments vs. Chloroplasts

What is the difference between Photosynthetic Pigments and Chloroplasts? I know plants have both but Prokaryotes (Bacteria and Archeae) only have the former.
6
votes
1answer
147 views

Alternatives to fittest-win and Moran processes as simple mathematical models of selection

When modeling selective sweeps as a micro-building block in models of macroevolution (not to be confused with misuses of this in creationist arguments), I use the fittest-win model of selection as a ...
6
votes
2answers
231 views

Is there such thing as a generic “metabolic type”?

A while ago I picked up a book called "Eat right for your metabolic type". The book discussed the effects of the endocrine system on the overall shape and the speed of metabolism. The book outlined ...
6
votes
2answers
719 views

For how long and how cold should I perform ethanol/isopropanol precipitations of RNA or DNA?

Precipitating nucleic acids using either isopropanol or ethanol is a very common operation, and I've read some very different protocols on the duration and temperature the precipitation should be ...
6
votes
1answer
652 views

How does water move throughout plants?

I haven't yet found a decent explanation for how water moves throughout plants. It does seem to travel more efficiently upward than out or down. Why is that? How does it travel through the plant?
6
votes
3answers
16k views

Does Human Female Meiosis II occur after fertilization with sperm?

I am reading the answer and I am getting confused by the sentence: At the end of meiosis I females have two daughter cells and meiosis II only occurs if and when fertilization occurs by a sperm ...
6
votes
3answers
2k views

Why does so much variation exist within species?

My last phrasing of this question did not go down well, so I will try again. The genotype of species is not always the same. If you ask yourself why not all of these possible expressions except one ...
6
votes
2answers
909 views

How does an inhibitory synapse communicate to the cell body of a neuron?

I picture a neuron as having multiple trees of dendrites attached to the cell body with a single axon leaving the cell body. I believe the cell body near the axon root makes the decision to fire or ...
6
votes
3answers
312 views

Where can I find the common names for the zoology taxonomy?

Forgive me if my question does not belong here or if I'm using incorrect terms, but I'm not educated in biology at all. I'm investigating the workings of the biological classification system. I was ...
5
votes
2answers
58 views

Is there a fundamental reason that plants cannot fix their own nitrogen?

Plants must have nitrogen to grow. According to the answer to this question, there are no plants can fix their own nitrogen (without the help of bacteria). Plants get their nitrogen in the form of ...
5
votes
3answers
421 views

How does protein denaturation work?

I was wondering how protein denaturation works. Are there covalent bonds, such as disulfide bridges involved, or is it based purely on non-covalent bonds such as hydrogen bonds? Why is denaturation ...
5
votes
2answers
78 views

Problems understanding membrane potential

I understand that membrane potential is the difference of the extracellular and intracellular ionic charges, due to their concentrations. We say that the extracellular space has a charge of 0 and then ...
5
votes
2answers
215 views

How is it possible to even hope for an ebola epidemic of this current size to remain contained to a relatively small part of Africa?

As of 2014-09-14 at least 5347 cases of ebola have been identitied. Some estimates are much higher. While experts are sounding the alarm to try to get sufficient resources to contain the outbreak, ...
5
votes
1answer
70 views

Scale up of Bacterial growth from 250mL to 1-5L?

I am currently growing up a specific strain of E.Coli with a knockout in 40mL of growth medium (LB) in a 250mL shaker flask... My ultimate goal is to scale up this process to a 1-5L large scale setup ...
5
votes
3answers
173 views

What are some examples of genes that code for multiple proteins?

The title pretty much says it all. It is widely taught that a gene in a eukaryotic system could produce more than one protein due to post-transcriptional modification, but I do not believe I have come ...
5
votes
1answer
309 views

Do ants within a colony ever fight or come to conflict?

I'm investigating how hierarchical groups of a larger organization with a common goal tend to come to conflict with one another over time. As you decompose a large group (for example a corporation), ...
5
votes
1answer
302 views

Empirical evidence for species selection

Do we have any empirical evidence in favor of species (or lineage) selection? Do we know some cases that can only be explained (or seem to be only explained) by lineage selection? What are today the ...
5
votes
1answer
905 views

Why do organisms excrete nitrogenous wastes?

All plants and animals excrete nitrogenous wastes in the form of urea, uric acid or ammonia. But isn't that a bit of a waste? I mean, there is a shortage of biologically available oxygen in the ...
5
votes
3answers
3k views

Why does it hurt when you take sweets, if you have cavities in your teeth?

When you have cavities in your teeth taking any sweetend drinks or food causes sensitivity or pain. What could be the reason?
5
votes
2answers
291 views

What are common causes of unexpected ligation products?

I digested two plasmids, one with EcoRI and AgeI and the other with EcoRI and XmaI. Digests looked as expected, so I purified the respective fragments and set up the T4 DNA ligation (AgeI and XmaI ...
5
votes
3answers
133 views

Finding a template/oligo combination for my first PCR experiment

I'm an information technology engineer. I love biology so I research biological topics and have an interest in PCR. That's why I have decided to create a PCR machine. Everything is done now and I ...
5
votes
1answer
622 views

How do individual plants adapt to changes in temperature?

If you grow a plant from seed in your nice warm house, then plant it out in your temperate garden, it will stop growing for a while, possibly die, and if not, subsequently resume growth. Standard ...
5
votes
1answer
69 views

How does the human liver regrow?

I understand that there would have to be a portion of liver present to regrow from, so how much liver would the minimum to be able to regrow? By what mechanism does regrowth occur?
5
votes
2answers
179 views

How does a pinched nerve cause pain (at the molecular level)?

Is this due to pressure differentials in the surrounding tissue? (Is it possible to have a pinched nerve without compression of the surrounding tissues, and does this cause pain?) What are the ...
5
votes
2answers
3k views

What is the function of nodes of Ranvier in axons?

In a neuroscience class I'm taking, it was explained that myelin covers axons in sections, the uncovered sections are called nodes of Ranvier, and signals propagate much faster in the covered ...
5
votes
2answers
292 views

How to find Exons in mRNA Computationally

I'm having trouble finding a method to find exons in the original DNA sequence used to create the mRNA, even given the sequence of the mRNA, as I cannot find a way to reliably identify the beginning ...
5
votes
2answers
713 views

The probability of indirect human fertilization?

What is the probability of conception in situations when sperm isn't directly ejaculated into the woman's vagina, like: The man's or the woman's hands come into contact with sperm, for example when ...
5
votes
2answers
430 views

Survival of E. coli on agar plate at -20 degree C without glycerol stock

If I kept E. coli transformed with plasmid at -20˚C without making glycerol stock, will it survive? Actually I have kept it this way for 18 hours. What will happen with it? Will it grow in fresh ...
5
votes
3answers
2k views

How did butterflies evolve to have eyes on their wings?

Some butterflies, such as the UK native Peacock butterfly (Google Image Search) have markings on their wings that look just like eyes, complete with a white fleck to imitate a convex, transparency ...
5
votes
1answer
182 views

What is an simple way to burn glucose for visible effect?

I want to make a partially working model of the digestive system that could digest complex carbohydrates. My ultimate goal is to be able to cut up some bread, put it into the model, operate it, and ...
5
votes
1answer
59 views

Why are some plants frost tender?

Why do some plants have the ability to stand frost, while others can't? Does it have to do with the size of the water vacuoles in the plant cells?
5
votes
1answer
5k views

How do SDS-PAGE gels differ in a Bis-Tris system vs. a Tris-Glycine system?

Protein migrate differently in Bis-Tris and Tris-Glycine gels. I was curious about the actual reasons why. Do certain gel systems result in a tighter resolution than others?
5
votes
1answer
1k views

What is the distinction between F' plasmid and R plasmid?

Is there a difference between an F' plasmid that has taken up a chromosomal gene that conveys antibiotic resistance, and an R plasmid? Is a bacterium containing an R plasmid and yet lacking an F+ ...
4
votes
1answer
51 views

Is it possible to dock multiple ligands with the target?

Is it possible to dock multiple ligands with a target? If so, what is the suitable software used for? -- Is it possible to fix specific docking region for the target (or) automatic?
4
votes
2answers
458 views

Are there any multicellular forms of life which exist without consuming other forms of life in some manner?

The title is the question. If additional specificity is needed I will add clarification here. Are there any multicellular forms of life which exist without requiring the consumption (destruction) of ...
4
votes
1answer
73 views

Mechanisms of extracellular stimulation of neurons

When stimulating a group of neurons with an electrode, let's say we put it in a region that contains axons, what is the mechanism by which the axons are stimulated? I've been told electrodes ...
4
votes
1answer
102 views

Mechanism of Muscle Growth

According to this video (sorry for the poor reference but it represents my level of understanding in physiology), muscle grow as a consequence of repairing micro-lesions. How are these micro-lesions ...
4
votes
1answer
73 views

Influence of temperature on transcription, protein binding and decay rates

I am the kind of biologist who doesn't know much about molecular genetics and about the dynamic of biochemical reactions. Question My question concerns the influence of temperature on the dynamic of ...
4
votes
2answers
480 views

Definition of “Effective population size”

Could you explain to me, what is the meaning of "Effective population size ($N_e$)"? I would appreciate an example as well.
4
votes
3answers
146 views

Why can't you just take a vitamin? Why you need a healthy diet on top of that?

From what I understand, your body needs certain amounts of vitamins and minerals to maintain health. Why can't we just take enough pills to obtain these vitamins and minerals?
4
votes
3answers
295 views

What is the mechanism of oxygen uptake in E. coli?

How does E. coli uptake oxygen? Most of the literature I found is concerned with response to oxygen level supplied in the medium, as opposed to how much is actually transported inside. Can they shut ...
4
votes
2answers
217 views

Where do the 31 spinal nerves start/meet?

Which part of the brain is the first place (from top to bottom) where all 31 pairs of spinal nerves (on each side) meet? Or if they all start at one place - where is that? Nominally, the beginning of ...
4
votes
2answers
153 views

Breakdown of energy expenditure at the level of a single cell

The metabolic rate measures how much energy an organism expends over a unit of time. Its breakdown for the human body in terms of its functions is well documented : so much for the heart, for the ...
4
votes
1answer
335 views

Why Isolated, recombinant plasmid DNA without any restriction is often detected in agarose gel electrophoresis as three DNA bands?

I don't understand why three bands are found without any restriction digest. Please answer me as soon as possible. Thank you
4
votes
2answers
106 views

A photosynthesizing mouse?

N. Shubin's Your Inner Fish makes the point several times that there is a lot of functional similarity between some seemingly remote gene cousins. If that needed reinforcing we have the spider-goat, ...
4
votes
2answers
303 views

Can animals make their own unsaturated fatty acids?

I know that animals can't make poly-unsaturated fatty acids (PUFA) and so require them from dietary sources. For eg.Omega -3 and Omega 6 fatty acids. My questions : Can animals synthesize other ...
4
votes
1answer
180 views

Why is Homo sapiens not categorized as an invasive species?

According to Invasive Species Advisory Committee (ISAC), the definition of the invasive species is “a species that is non-native to the ecosystem under consideration and whose introduction causes or ...
4
votes
1answer
1k views

Rate of cell division in humans

On average, how many cells divide each day in a human being? How long does a cell wait before dividing itself ? I have tried to look on the internet but surprisingly the answer is difficult to ...

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