9
votes
1answer
699 views

Why some parts of the human body have immune privilege?

Why have the eye and CNS have immune privilege? Why does the body not develop tolerance against their tissue and instead risk their damage in case an accidental immune cell infiltration? Wiki: ......
9
votes
2answers
182 views

How much energy does a cell expend maintaining its contents?

In software engineering, an analogy is sometimes made using biological cells. I would like to know whether it has basis in fact. People say (Alan Kay was first) that "objects" in software should be ...
0
votes
1answer
105 views

What is meant by clonal isolates?

With respect to this website: http://horfdb.dfci.harvard.edu/ what does the term clonal isolates mean?
6
votes
2answers
671 views

The effect on the efficacy and potency of a non-competetive antagonist binding to the active site of the receptor (dose-response curve)

According to the book "Principles of Pharmacology: The Pathophysiologic Basis of Drug Therapy" by Golan et al, non-competetive antagonists can bind to both the allosteric site and the active site. I ...
4
votes
3answers
333 views

Amino acid compatibility

The (human) genetic code encodes 20 amino acids. They form a protein using peptide bonds. Each amino acid has a carboxyl group (COOH) and an amino group (NH2) that can potentially form a peptide bond. ...
1
vote
1answer
46 views

How many NOD like receptors in Human?

This is pretty specific question maybe. Anybody have an estimate? For Toll Like Receptors there are something like 10... http://www.jbc.org/content/276/4/2551.long I'm only finding NOD1 and NOD2 => ...
2
votes
1answer
88 views

White blood: cells concentration

Anyone knows of a table with average concentration that can be found in white blood? Something like: Neutrophils - 80% Mast cells - ?% Dendritic cells - ?% B cells - ?% Helper T cells - ?% Killer ...
1
vote
1answer
109 views
25
votes
4answers
14k views

Can humans eat grass?

Can a human eat grass and digest it? Could it be possible to use it as food just like other plants such as wheat or beans?
1
vote
0answers
166 views

rRNA colony PCR amplification not working

We used some universal primers to amplify rDNA in a certain isolated bacterial specie. The problem is that rDNA PCR is not amplifying. Primers were working well in other strains of same bacterial ...
2
votes
1answer
214 views

Why are Oxyuranus snake species named Taipan?

It is interesting, why are Oxyuranus species called Taipan. The snakes are Australian but taipan looks like of Chinese origin word (it means big shot - important person).
0
votes
2answers
61 views

Mathematical modelling of omics data [closed]

I'm interested in modelling host-pathogen interactions using mathematics. I know there are good resources in Pubmed but seems I'm looking for a book or introductory reviews. My background in maths is ...
2
votes
1answer
52 views

How does myopia actually work?

This is potentially more or less a question about optics, but I've never been truly satisfied with any explanation that I've been given about Myopia. In fact, I almost can't even believe it. There are ...
0
votes
1answer
49 views

Question about contents of small bowel

I know, or at least I think I do, that the contents of the stomach are called chyme and that it is acidic. What are the contents of the small bowel called and is it acidic?
4
votes
1answer
64 views

Why MHC class I antigen presentation exist in normal cells?

The book "Kuby Immunology" states that normal infected cells might present antigenic peptides on their surface via MHC class I, but these can't activate naive CD8 cells. Only infected professional ...
9
votes
1answer
2k views

Why is the brain dependent on glucose?

The strict dependence of the (human) brain on glucose has always been puzzling to me. While ketones can substitute for a portion of the brain's energy needs, it cannot substitute completely: blood ...
0
votes
1answer
46 views

Downstream analysis after in vivo pathogen RNAseq

We performed RNA-Seq of in vivo bacterial samples and identified some key up- and down-regulated pathways. We compared bacteria during infection with conventional agar plate. Which could be the next ...
0
votes
1answer
65 views

Would it be more genetically efficient to have shorter lifespans?

If, for example, humans had an average lifespan of, say, 30 years, and we reached sexual maturity at age 10, then we could "evolve" more quickly and theoretically "weed-out" more genetic diseases ...
1
vote
3answers
85 views

DIY storing family DNAs' samples for future uses (eg medical)

I have a question I could not get an understandable reply from Google and I am no expert in the matter, so my plead to you is if you could give me practical and relatively easy to follow advice. With ...
4
votes
2answers
370 views

Antibodies stored in freezer instead of fridge

Someone from my lab put the box of antibodies in the freezer instead the fridge. Can I hope that they will still work?
7
votes
2answers
2k views

What chemicals and structures control the *direction* of plant growth in leaves, stems and roots?

If you want a specific plant, let's say the snap peas I am growing that I bought at Agway. I noticed that they grow towards the light source through the nearby window. Also I think roots work the same ...
1
vote
0answers
27 views

Are upstream activating factor (UAF) and upstream binding factor (UBF) the same thing?

During ribosome pre-40S and pre-60S synthesis, many sources state the importance of UAF or UBF in initiation complex of ribosome DNA transcription. None of the sources I've seen mentions the other ...
2
votes
1answer
60 views

Very introductory online source of information in evolutionary biology

We receive quite a lot of questions from layman in evolutionary biology in this site that are sometimes difficult to answer just because there is way too much to say. Why don't human keep evolving? is ...
2
votes
1answer
107 views

Mitochondrial D-Loop

I know that the D-loop is a DNA complex in which the strands of double helix DNA molecule are separated for a stretch and held apart by a third strand of DNA. Usually, this third strand has a base ...
3
votes
2answers
427 views

Why are some housekeeping genes considered better?

Whenever a PCR is done, we have to use housekeeping genes like GAPDH, to self normalise the values to account for different starting cDNA/DNA. Now there are many different genes like GAPDH, ubiquitin, ...
3
votes
0answers
43 views

Infrared emitting diode eye safety

I'm thinking of creating my own laser tag system and am a bit concerned about the eye safety aspect. Most of the homebrew system I've seen use the TSAL 6100 diode(http://www.vishay.com/docs/81009/...
0
votes
0answers
32 views

did pteranodons live in colonies?

In one book I read that pteranodons lived in huge colonies. But pteranodon's family in "Dinosaur Train" ( https://en.wikipedia.org/wiki/Dinosaur_Train ) does not seem to be a part of any colony. So, ...
0
votes
1answer
48 views

Why do teeth decay of sugar?

Sugars is a common component in diet of mammals. Why then their teeth did not develop resistence to sugar decay?
2
votes
1answer
102 views

Does creatine-phosphate (CP) supplementation regenerate NADH?

I have the following facts: It is possible to convert ATP <-> creatine vice-versa. (ref - non scientific) CP supplementation protects against metabolic syndrome. ref1 ref2 Fructose digestion ...
3
votes
1answer
61 views

Glutamine analysis

I use to run a method to analyse all the amino-acids in a food sample. For that I have to hydrololyse the sample and in the last stage of the method I read the amino acids with a ion exchange ...
1
vote
1answer
63 views

What term describes insects that must eat protein to reproduce?

I know there is a specific term for insects that need to consume protein to produce viable eggs, as well as a term for those insects that don't need to consume protein to produce viable eggs.
1
vote
0answers
412 views

Does malaria have a reservoir host?

Can warm blooded vertebrates other than humans act as reservoir hosts for malaria parasites? I'm mainly interested in Plasmodium vivax and possible reservoir hosts in the wider area of Europe/ Eurasia....
2
votes
1answer
32 views

Which is the guideline to choose a molecular target to identify vertebrate hosts from arthropod bloodmeals?

There are some molecular targets to identify vertebrate hosts from arthropod bloodmeals including the Cyt b gene and the COI gene. Which are the standards or characteristics that I have to bear in ...
2
votes
4answers
600 views

Why *morning walk*?

My PT teachers professes working-out/training in early morning hours is the best time to do so, a quick search on benefits of morning walk (or some similar phrase) reveals a lot of information which ...
2
votes
1answer
53 views

In insects, does the Alanine repeat occur on the homeodomain sequence of the abdomen or does it occur on a different sequence?

By "Alanine repeat", I am referring to the suppression of the formation of extra insect legs due to Ubx gene suppression through Distal-less repression.
1
vote
1answer
532 views

An example for N50? Why do we need it?

I'm trying to understand N50 on the wikipedia. But I was unable to get a sense of the definition: Given a set of contigs, each with its own length, the N50 length is defined as the length for ...
7
votes
2answers
767 views

Solvent Accessibility, the 20% cut-off method

I'm reading the papers linked below and all three of them mention a 20% cut-off for buried/exposed residues, by calculating a relative solvent accessibility (RSA) value. I understand how the RSA is ...
1
vote
1answer
4k views

How is ATP involved in muscle contraction?

The sliding filament mechanism as explained by my text does not elaborate on how ATP is involved in the cross bridge binding and contraction process. How does muscle contraction utilize ATP? In my ...
2
votes
2answers
55 views

How to compute the regression of individual fitness on individual phenotype

Consider a population structured in groups of two individuals. Individuals' interactions follow an additive prisoner's dilemma: \begin{array}{c |c |c|} & C & D \\ \hline \text{Cooperate (} C \...
0
votes
1answer
58 views

Without help of Insects, can plants & trees bear fruits & flowers? [closed]

Without help of Insects, can plants & trees bear fruits & flowers?
4
votes
1answer
66 views

Are stem cell lines cryogenically preserved or commercially available like HeLa cells are?

There are HeLa cells, which were taken from sick cancer patient and now is growing worldwide in different laboratories for experiments. HeLa cells can be kept because they are immortal and so they are ...
1
vote
1answer
154 views

Interlocus Contest Evolution means suicide?

I was reading Matt Ridley's "Genome" book. I am trying to understand the Interlocus Contest Evolution in his X-Y chapter. I do not understand why the X and Y chromosome would want to kill each other. ...
4
votes
2answers
56 views

Dyes to bind to double stranded DNA?

Are there any commercially available fluorescent dyes that will bind only to double stranded DNA (not RNA, single stranded DNA etc.) for studying in vitro using confocal microscopy?
1
vote
2answers
302 views

Microscope scale bar

In some microscopic images, there is a bar scale at one of the corners or near a specific object. How are they added to the images? Does the slide have some markers, or are they digitally added? If so,...
1
vote
1answer
84 views

History: Building a “Phylogenetic network” of famous evolutionary biologists [closed]

Seems like many of the famous evolutionary biologists are somehow related to each others. For example (and I might be mistaken), Ronald A. Fisher was mentored by a descendent of Charles Darwin. Joe ...
5
votes
1answer
299 views

Influence of eating at late night on sleep

I have often heard the saying that one shouldn't eat a mountain of food because blood will go to the stomach and it'll be difficult to sleep well because the stomach can't rest at night. I see that ...
4
votes
1answer
205 views

How could I identify whether given RNA-seq data is paired end or single end

I need to have a RNA-Seq dataset and therefore, I've visited the following site NCBI-geo C. Elegans In the Supplemantary file part, I clicked the SRP/SRP051/SRP051702 ftp and downloaded sra file. ...
1
vote
1answer
3k views

What's the difference between stabilizing selection and balancing selection?

I came across these terms in Darwin's "Origin of Species" and I wasn't sure what the difference is.
0
votes
1answer
133 views

What does this mutation notation mean: MAP2K41161fs*12

I received a report that had a mutation notated as: "MAP2K41161fs*12". I'm used to seeing this sort of thing: BRAF(V600E) or BRAF(T1799TA). Can someone tell me how to interpret "MAP2K41161fs*12" ?? Is ...
3
votes
5answers
1k views

Does any animal species have two sexes and more genders?

Does it exist any animal species that have two sexes and a kind of different internal diversification in two genders or more? I mean no something like different-task-based diversity but something ...

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