-1
votes
1answer
79 views

Why do mammals in the wild retain the “trait” of menstruating with blood?

This question intrigued me a lot since if animals in the wild did have menstruation with blood flow, wouldn't all that blood be attracting every other predator in the vicinity?
3
votes
1answer
80 views

mRNA with Pseudouridine and 5-methylcytidine?

I am making mRNA by in vitro transcription for transfection purposes. There is a lot of literature about how protein expression is increased and innate immune activation is decreased by substituting ...
4
votes
2answers
339 views

How does the brain know where a signal came from? What is the addressing system

I am an electronic engineer so I am thinking about this from an electronics outlook. How does the addressing system work, As I see it, the nervous system is small parallel branches attached to larger ...
2
votes
1answer
277 views

Regarding kidney transplant

One of the options is correct Kidney can be taken from a dying person who has the a) cessation of neurological function only b) cessation of cardiac function only c) cessation of ...
1
vote
1answer
35 views

Difference between Fulminant and Acute Meningococcemias

Fulminant seems to mead rapid. There is however some articles with both phrases: "fulminant meningococcecemia" (about 5000 Google matches) and "acute meningococcecemia" (about 3000 Google matches). I ...
1
vote
1answer
148 views

How to relate human metabolic model reactions and cancer drug targets / reactome items?

Trying to find a way to take a cancer drug (from CancerDR, for instance) and infer the metabolic reactions that are affected by it in the Human Metabolic Model. Essentially, I would like to know ...
0
votes
1answer
21 views

Is Rheumatic fever more Chronic than acute?

It follows from the complication of S.pyogenes' pharyngitis. I am thinking how the inflammatory response behaves: acute or chronic or something between. I think chronic disease is better description ...
4
votes
3answers
205 views

Why do we need deep sequencing?

Why do we need deep sequencing? Why cannot the sequencing technologies read all the nucleotides correctly at the first read? Sorry since this question is too trivial, I don't have a biological ...
0
votes
1answer
31 views

Is endoderm visible in the germ layer?

This picture is my drawing about germ layer - not embryonic folding as I wrote initially. Where exactly is the endoderm here in the picture? The known things Ectoderm Neural tube Notochord ...
3
votes
1answer
39 views

Prevalent large (>=90kDa) maintenance protein/loading control

I was wondering if anyone had recommendations for good, large (hopefully 100kDa+), control proteins that would be present in most mammalian cells. I'm working mostly with tissue samples from humans ...
0
votes
2answers
139 views

Chills down the spine with certain music?

I am sure many of us know the feeling of that tingling chill down our spines when listening to certain music. I experienced this most when my younger brother played an emotional piano piece for a ...
0
votes
1answer
60 views

How do neurons send electric pulses even though they are surrounded by liquid which is mostly water?

I just thought about the fact that people move through electrical pulses traveling through neurons in the brain and muscles through the body. Here is what i know: the body is full of 97 or more, ...
1
vote
1answer
74 views

Enzymatic Protein Deglycosylation of TCL

I've done O- and N- linked enzymatic deglycosylation of purified and lyophilized proteins. Following some modifications to the manufacture's protocol, I had great results. I now want a student to ...
1
vote
2answers
78 views

Aren't current explanations for the evolution of human cooperation a little too reductionist?

Lately, I've become a believer in the limits of reductionist explanations especially in areas like complex systems and biology. So, without wasting any more time, I'll get to my question.. Whenever ...
2
votes
1answer
105 views

Mutation rate in viruses

Mutation rate is a phenotypic trait that evolves. The process of evolution of such kind of traits are often referred to as evolvability. I am wondering about the evolution of the mutation rates in ...
2
votes
1answer
44 views

Do vitamins help our memory?

It is commonly believed that vitamins can help our memory. Is this really true? What is the biological basis of this? Is there any specific research paper on the subject?
4
votes
1answer
4k views

Why do sneezes come in pairs or more?

I very rarely sneeze just once, usually there's two coming in a row, and I've noticed the same thing in other people and even in animals. Is there any reason for this?
3
votes
2answers
223 views

How do I create a probe for in situ hybridization?

I tried to make the probe several times but it failed again and again. It usually turns out that the probe after hydrolysis is very very short (maybe around 50nt). I did not check the RNA before ...
0
votes
2answers
156 views

DNA sequence from the middle of a gene

Someone gives you a short DNA sequence that comes from the middle of a gene. 5'- TCTAACTGATTAGC -3' 3'- AGATTGACTAATCG -5' From this sequence, determine the ...
0
votes
1answer
63 views

Why prefer $MgSO_4$ over $MgCl_2$ in recipies [closed]

Many molecular biology recipes use MgSO4 (and not MgCl2). Is there indeed a preference? If so, why?
4
votes
1answer
47 views

Is the regulation of lactose operon different between Gram + and Gram -?

I know that in E. coli the lactose operon is shut down by CAP protein when binding cAMP. Is this true also for Gram positive bacteria?
10
votes
1answer
29k views

Why do we add salt when precipitating DNA?

All the DNA extraction protocols I have seen involve adding salts to the extraction buffer. What is the purpose of the salts? What happens if they aren't included?
2
votes
2answers
368 views

What modern insects are not (flying OR descended from flying ancestors)?

What modern insects are not flying or descended from flying ancestors? (I read somewhere that 99% are, so of course I instantly became curious which aren't. xD ) EDIT: Sorry, I should have been ...
3
votes
4answers
153 views

Translation of mRNA?

We inserted an insulin gene from human into bacteria. Will translation of the gene (protein formation) occur in bacteria? If translation occurs then why does it occurs, give a reason for this?
2
votes
1answer
88 views

How usable is the Human Metabolic Model to predict biomass?

In order to use the Human Metabolic Model for Flux Balance Analysis of specific cancer cell lines, we would like to know what sort of flux values have been determined for the Human Metabolic Model. ...
1
vote
1answer
42 views

What are good data on the Human Metabolic Model and where can I get them?

Trying to get a good SBML representation of the Human Metabolic Model for use in Flux Balance Analysis and drug targetting (i.e. gene knockout) simulations. What are good sources for these data? ...
1
vote
1answer
95 views

Where can I find the metabolic network for the human erythrocyte?

Where can I find the metabolic network of the human erythrocyte (red blood cell), in SBML format? The red blood cell metabolic network is a model that's usually employed in the literature [see refs ...
0
votes
2answers
236 views

How to determine the actual flux in the human metabolic model of a cell line?

The problem to be solved is to determine what the flux values are for the different reactions in the human metabolic model. As far as I understand, a good way to do that would be to use gene ...
0
votes
1answer
589 views

What is splice junction pairs?

Splicing is a modification of pre mRNA when all introns are removed and exons are joined. What is a splice junction pair? It is two exons which connected together?
1
vote
3answers
427 views

What would happen if you combined the world's deadliest diseases & viruses?

What would happen if you took the deadliest diseases/viruses in the world and combined them in a single medium (a solution of water or a test subject)? Would the strongest virus defeat the rest or ...
1
vote
1answer
74 views

Is the Andraka pancreatic cancer test real?

In the wikipedia article about Andraka's pancreatic cancer test there are some controversial statements. On one hand there are many glorious words about the method, also some awards, also some ...
4
votes
1answer
190 views

Does playing music during sleep actually suppress rather than rouse the brain?

I've long been interested in the effect of music/sound on dreaming, and even built 2 apps that play music during REM period. Now I'm looking at this article about FMRI studies of a sleeping brain, and ...
2
votes
1answer
88 views

Does this equation have a typo? (Systems Biology, singular value decomposition) [closed]

I'm reading a systems biology paper, and I'm suspecting there's a typo in an equation, but I want to make sure. The article is "Systemic metabolic reactions are obtained by singular value ...
2
votes
1answer
1k views

Identification - What kind of animal leaves these tracks?

I live in the suburbs of Washington DC where it recently snowed. I was admiring all of the tracks left behind by wildlife of all kinds when I came across these prints, that I was unable to recognize ...
-1
votes
1answer
69 views

Late control mechanisms of blood pressure (arterial) level? [closed]

I first covered the fast and moderate control mechanisms which I think are Fast control: Baroreceptive reflex (tonus), Chemoreceptive reflex (CO$_2$). Moderate control: Bicarbonate (ECF), ...
2
votes
2answers
106 views

Why do people use anti-inflammatory drugs?

Why people should take anti-inflammatory drugs, when inflammation is a physiological way to defend our organism?
1
vote
1answer
91 views

Do “transcription factor binding site” and “operator” mean the same thing?

Can the terms "transcription factor binding site" and "operator" be used interchangeably in all contexts when referring to a DNA sequence, e.g. regardless of a cell type, whether or not the binding ...
0
votes
1answer
3k views

Which processes are during the latent period of ventricular contraction?

The latent period is the time between the start of depolarisation and the start of contraction. I know that the contraction of ventricles starts after sufficient influx of Ca2+ and threshold ...
1
vote
1answer
36 views

Dinuclei cellular mechanism

I am no biologist, but I have this question buzzing in my mind. It's a matter of curiosity What happens when two nuclei occupy the same cell? Would we expect the embryo (if it lives) to have complex ...
0
votes
1answer
195 views

vestigial structure question

If a species has a vestigial anatomical structure that it no longer uses, would you expect this structure to disappear over many generations through the action of natural selection? Always, sometimes, ...
1
vote
1answer
126 views

Is end-systolic volume (ESV) the same as residual volume?

I am reading this site and my study materials. The end-systolic volume (ESV), 50-60 ml, is the volume of blood found in ventricles after systole and contraction of ventricles. I think ESV is the same ...
2
votes
1answer
118 views

Cloning of a sheep.. Dolly?

Dolly the sheep was a clone. When she was born she was eight years old on her 1st day of birth, her mother (from which she was cloned) was also eight years old. What is a molecular reason behind this ...
1
vote
1answer
44 views

Book on yeast reproduction, evolution and on the use of yeast as model organism

I am looking for a good book in: Yeast reproduction mating types Recombination mating type determination mechanism Yeast evolution Yeast as model organism and its use in experimental biology It ...
1
vote
0answers
32 views

How are lophotrichous flagella of helicobacter pylori produced?

I am thinking the mechanism is something like first adhesins (mucinase). Howevever, this does not seem to be enough. How are the flagella of H. pylori produced?
1
vote
1answer
75 views

Evolution, self organization and neuroscience

I have rudimentary knowledge of evolution, and biology in general, so bear with me if this question is a bit naive. Let's say we have a particular trait, like highly sensitive peripheral vision. ...
2
votes
2answers
328 views

Why are transcripts translated from 5' to 3' direction?

What is the mechanism? In other words, how does the tRNA know whether the codon is in 5' to 3' direction? I also heard that anything that is translated from 3' to 5' direction is degraded. Is it true? ...
1
vote
0answers
40 views

How far in evolutionary distance is the P-distance estimator “accurate”?

I'm reading Yang's computational molecular evolution and in the very first chapter it says the P distance is a "simplistic distance measure" between two sequences. It is the number of different sites ...
1
vote
1answer
18 views

Do Yersinia genus spp have anything movement factors?

It can spread in blood (Type III secretion system -> apoptosis). I am interested if there is any others ways to move. Do Yersinia spp have anything virulence factors to move?
2
votes
1answer
97 views

2 blue eyed children

My husband had light brown eyes. His father had hazel and his mother, light brown as well. His younger brother however, has blue eyes. Both my children have blue eyes. Is this possible? I thought once ...
1
vote
1answer
78 views

Pseudosexual reproduction

Research projects with artificial life generally use creatures that are haploid. The offspring randomly inherit some genes from one parent, and some genes from the other parent. Q1: Are there any ...

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