2
votes
1answer
108 views

Pectinase Enzyme Assay

I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the ...
8
votes
2answers
208 views

Is this a plant or a fungus?

It was soft and covered in flies. My guess is fungi. Location: South Florida.
3
votes
0answers
33 views

Do I need to use the same WT C57BL individual for back-crossing or can I use a new one for each generation?

I've just received heterozygote C57BL mice from COMP and I need to start back-crossing them. I've read many colony management protocols, but couldn't understand if I need to use the same WT mouse for ...
3
votes
2answers
255 views

EM Brainwaves VS Brain Wave (EEG) [closed]

The brain purportedly produces very weak EM waves. EEG is a method of measuring electrical brain activity, it has classifications for the types of brain wave it can detect: Theta, Alpha, Beta, Gamma ...
12
votes
1answer
381 views

Can anyone identify this bird?

Can anyone identify this bird: it looks like a zebra finch but they are not native to UK are they? Photo taken from my garden in Shoreham, West Sussex on 16/8/2014.
7
votes
4answers
2k views

Why do obese people tire quickly?

Fat people have a large amount of calories (energy) stored, but I have noticed all time when they do physical activities they get tired fast in comparison to fit people - why does this happen?
4
votes
2answers
51 views

Compatibility drugs info

Let's suppose that I take a DrugA for ProblemA and then I got this ProblemB and started to take DrugB. Are there general rules I can look up to figure out whether I can take DrugA & DrugB ...
7
votes
3answers
5k views

Why does replication require primers while transcription does not?

In transcription, there is no need for any primer. I guess the basic mechanism of DNA polymerase & RNA polymerase is the same. So why does replication have the need for a primer?
1
vote
1answer
85 views

What are the dimensions in angstrom of bacteria? [closed]

I'm going to take a picture of a bacteria but I am unsure how large it is. What are the dimensions in angstrom of bacteria?
20
votes
3answers
2k views

Why is high fructose corn syrup unhealthy?

I understand that high fructose corn syrup was found to have detrimental effects on peoples' health. Why is this so?
6
votes
1answer
85 views

Why ml scale blood retrieval is required to run a blood test?

Substances found in blood are present at microscopic scale and tend to be invisible to the unaided eye. Why is a whole vial of blood (at ml scale) is required to assess the presence and concentration ...
4
votes
2answers
367 views

positive and negative feedback

I want to know more about positive feedback in gene regulation and to know the similarity and differences between positive and negative feedbacks.
5
votes
1answer
114 views

Can the negative afterimage appear only if there is light or is it possible in darkness?

Reading the following paragraph: After staring at the red and blue shamrock, you saw a green and yellow afterimage. Opponent-process theory proposes that as you stared at the red and blue ...
5
votes
1answer
107 views

What is a good identification key for European mushrooms?

I live in the Netherlands, and I am looking for an identification key for mushrooms. Are there any keys you could share?
7
votes
1answer
148 views

Why are recombination rates increasing in mammals?

I have recently become fascinated with an awesome topic in biology and evolution that I feel is rarely covered in biology courses. That is, rates of meiotic recombination, or the the amount that an ...
4
votes
0answers
62 views

Is there a more updated version of the book “Genome?” [closed]

I read the book Genome by Matt Ridley for my AP biology course. I found it fascinating, although it seems another book must have been written on the topic since 1999. Anything you can recommend?
2
votes
1answer
67 views

Percentage of mutations caused by radiation?

Consider a gamete in a typical human being. In the interest of being specific, let us say that we're talking about a typical ovum in a typical middle class woman living in a developed nation. What ...
3
votes
1answer
726 views

How much time does it take a human body to replace all of its atoms?

Mikel Vsauce (Vsauce youtube channel) said in his video that it takes about 5 years for the body replace all of its atoms, while in asapscience youtube channel they are claiming that it takes 10 ...
5
votes
1answer
7k views

Do human anal glands have any function?

Anal glands in all kinds of animals play a range of diverse functions - providing fat for preening feathers or waterproofing fur, generating distinct scent, even repelling predators. None of the ...
3
votes
1answer
822 views

Mitochondria - are they really separate organisms that once merged into eukaryotic cells?

Theoretically, mitochondria are said to be a separate organism that is concerned with its own life and its own processes. In fact, it even duplicates individually. I know a similar question is here ...
12
votes
1answer
2k views

Is there any relationship between heartbeat rate and life span of an animal?

Is there any relationship between heartbeat rate and life span of an animal? Do they belong to a cause-and-effect relationship or are they both caused by some phenomenons or a common cause?
14
votes
3answers
6k views

Why isn't vomit corrosive?

Vomit comes from gastric acid, right? And in gastric acid there is HCl and HCl is corrosive (strong), how come when I vomit, it doesn't destroy things that it (as the vomit) hits (such as floor, ...
5
votes
1answer
807 views

Help me Identify this Spider

This red spider has set up a web in my yard, in South Eastern, Texas, United States: The spider itself is about an inch long (2.5 cm), and is predominantly red to brown in color, with red legs, and ...
2
votes
0answers
103 views

Human body control? [closed]

What can we consciously control in our bodies besides our muscles and brain (thought, memory, etc.)? Is the notion Bio Feedback related to this matter? EDIT: I mean I can move my muscles at will but ...
2
votes
2answers
533 views

Using RNA-seq to compare gene expression across patients instead of between Control and Experimental conditions

I am working with RNA-seq data from the Cancer Genome Atlas TCGA and I have been reading about how people have compared gene expression levels measured by RNA-seq. Many of the papers I have read talk ...
5
votes
1answer
375 views

Where can I find the pMON7124 plasmid sequence?

I need the map of the pMON7124 plasmid. As this is not easy to find, I welcome any hints.
6
votes
1answer
380 views

DNA extraction from agarose gel

Because DNA is soluble in water, is it possible to extract PCR product by dissolving excised gel containing the DNA band of desired length in water, mashing the gel piece with a pestle, centrifuging ...
5
votes
3answers
297 views

What is the point of having evolved two nostrils?

How would having two nostrils be advantageous to your sense of smell or your ability to breath? I already hypothesized that either one of the nostrils could act as a back up for when another gets ...
3
votes
2answers
884 views

In practice, do fishes die from old age?

I was watching sea birds eating fishes and wondered if fishes actually died from old age, or if they are all eaten before this can happen? I reckon some big predator fishes must be able to live long ...
2
votes
2answers
215 views

What does this Barbary Macaque facial expression mean?

A girl pretended having a piece of food in her hand and pointed it to a Barbary Macaque in Gibraltar. As a response he made this facial expression to her. What does this mean?
3
votes
1answer
62 views

Can a plate with incompletely filled LB agar be used?

I am falling short of LB agar plates and I have a plate with less LB agar poured. I meant, the plate is not fully filled with the agar (it was the last plate I poured the medium into). Some part of ...
9
votes
1answer
228 views

Can a bacteriophage be used to treat bacterial diseases?

Some bacteriophages reproduce using the lytic cycle which ends with the destruction of the host bacterial cell. I was wondering if theoretically this could be used theraputically to treat bacterial ...
2
votes
0answers
40 views

Assembly reconciliation

We have some bacterial genomes that were assembled using Spades, they were sequenced with and IonTorrent PGM. There are many assemblers and they give different results. I was interested in a tool ...
3
votes
0answers
173 views

A Genetic linkage problem [closed]

I have recently switched from electronics to computational biology. So having some hard time with my homeworks. Can someone guide me on this problem? Consider 6 genes A, B, C, D, E, F, G. Some ...
2
votes
1answer
45 views

What is the biding site code recognized by the parts of the spliceosome

Another question about another Youtube video. At 0:50, the splicing process begins to remove the non-coding section of the DNA (intron), so the different parts of the spliceosome attach to the borders ...
1
vote
2answers
62 views

Periodic Muscular Growth in Humans

If a healthy human is at a certain muscle state and then experiences muscular atrophy solely due to disuse/lack of exercise, is it easier to gain that muscle back than if the human had never reached ...
6
votes
1answer
94 views

What regulates the timing of the motion of molecular machines during DNA Replication?

This question is about this video I found on Youtube. I just want to know what is the mechanism which regulates the timing of motion of the parts of these molecular machines. I know that those big ...
4
votes
1answer
1k views

DpnI over-digestion

We have a long protocol that we are optimizing that includes DpnI digestion of a PCR product (to remove any of the template DNA if it's methylated, and while we're not certain in the blind tests, ...
5
votes
3answers
853 views

How does strength of a pulse related to EKG(ECG)?

There are variabilities in the heart rate as well as strength of heartbeats. I want to know if one can predict which heartbeat will be strong or weak based on the corresponding EKG/ECG signal. I am ...
3
votes
1answer
57 views

What are the benefits of statins in terms of prolonging life?

Recently the UK government suggested that all adults over the age of 50, without exception, would benefit from taking statins. I have an elderly female relative who may, or may not have had a minor ...
3
votes
6answers
4k views

are sperms considered living [closed]

recently i got into a debate with this question on hinduism.se , as the link given above shows, are sperms considered as living or non-living as far as my knowledge is concerned, sperms undergo ...
1
vote
1answer
72 views

What is an effective method for putting on blue rubber butyl stoppers?

They look like this. We have to do a lot of gas sampling and these are really difficult to assemble. I've been scouring the internet for advice but there are none. Do you have experience with ...
3
votes
0answers
95 views

Can ants learn things?

I have an ant nest in a jar. Whenever I want to observe them, I have to remove the cover and therefore put them in light - that scares them. They also can see shadow cast by myself. I often throw in ...
7
votes
2answers
3k views

Why is oxygen needed for the electron transfer phosphorylation?

I understand that oxygen is the acceptor of electrons and hydrogen ions during the electron transfer phosphorylation, the last step off the ATP-producing aerobic respiration. But why? Aren't there ...
4
votes
1answer
7k views

Difference between intervertebral and vertebral foramina

In the human spine, what is the difference between intervertebral and vertebral foramina? Is it just a matter of perspective and do they refer to the same thing or are they different?
4
votes
1answer
183 views

What are possible reasons for a DNA template not giving amplification?

I am trying to amplify ~10kb length fragments using NEB LongAmp Master Mix. I had been using one particular aliquot of DNA (template amount in each PCR reaction of 10ul being around 60ng) and getting ...
7
votes
1answer
133 views

How was the cama's life expectancy computed?

A cama is a hybrid between a male dromedary camel and a female llama. The first cama was born on January 14, 1998, yet on the Wikipedia page it is said that a cama's life span is 30–40 years. How was ...
6
votes
1answer
578 views

Evolution of umbilical cord and reason of its elongation

To answer a question myself, recently I've read that Years and years ago, a long umbilical cord enabled a woman to grasp her baby after birth and run away from predators" Update: $Question:$ ...
1
vote
1answer
280 views

What are the main differences between lab-grown tissues and natural tissues from living animals?

What are the main differences between lab-grown tissues and natural tissues from living animals? Using a biologist's classic "structure (anatomy) and function (physiology)" idea, I thought about the ...
3
votes
1answer
145 views

The smell from eating asparagus

I am wondering about one thing. I have read different accounts of this (prior to reading it on Wikipedia), so I am wondering. Here are the two versions I have read: asparagus leads in a certain ...

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