7
votes
1answer
148 views

What is this creature?

Since it has six legs, I believe it's some sort of insect, but I could be wrong. I spotted it in my Central Texas backyard (Austin area). It was very slow and not at all skittish. For size reference,...
0
votes
2answers
606 views

Can viruses that normally infect eukaryotic cells also infect bacteria?

Can standard viruses infect bacteria? I'm not speaking of bacteriophage but typical RNA and DNA virus such as influenza and Ebola.
2
votes
1answer
105 views

can a bacterial protease inhibitor cocktail be used for Western Blotting involving HUVEC cells and HT-29 adenocarcinoma cells?

Dear fellow biochemists, I need some advice on Western Blotting, more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore ...
1
vote
1answer
377 views

Does E. coli survive at any pH level?

Does E. coli survive at any pH level? If I was to incubate it in agar of different pH, would it still form a bacterial lawn as it's called?
3
votes
2answers
355 views

19th century Latin species description

While reading a 19th century text of new species description, I'm confused by some Latin phrases. The case here is: Corp. long. 8 poll., caud. 9 poll. I guess 'corp.' and 'long.' are ...
2
votes
1answer
68 views

Modelling how much cadmium a cell will be able to chelate with phytochelatins

I am involved in a synthetic biology project where I have to do modelling with ODEs. The biological process I want to model is the chelation of Cd2+ with phytochelatins, to know how much can be ...
1
vote
0answers
34 views

Is it possible to convert nerve signals or electrical pulses into a readable format? [duplicate]

Can we have the technology to recreate the informations (like images, sounds etc.)[as in the form of signals in our mind] from human mind to human understandable format? In short, can we read human ...
2
votes
1answer
86 views

NMR spectroscopy detection methods for proteins

While studying, I've stumbled upon a table comparing the sensitivities of different detection methods in NMR spectroscopy: "Direct", "DEPT" and "Inverse", where "Inverse" seems to be the most ...
2
votes
1answer
43 views

How to identify the genes that distal enhancers pair?

I am writing a project proposal and I have to talk about this problem: how to identify the genes that distal enhancers pair? I am really new to this topic and I don't know what it is all about. I ...
3
votes
1answer
115 views

How do nicks in the DNA strand affect the success of Long Range PCR?

Long Range PCR using NEB Master Mix - Hot Start Taq was working fine for me (amplicon sizes of ~10kb) but stopped working all of a sudden. Is it possible that many freeze-thaw cycles on the DNA ...
2
votes
0answers
51 views

What should be the distance between plant promoter and gene?

I am trying to clone a rice gene under a different endogenous rice promoter.I will be cloning the CDS of the rice gene.So I wanted to know what is the minimum or maximum distance I should put between ...
2
votes
1answer
584 views

Enzyme Assay, absorbance change to concentration change

I have a few issues with this question. An enzyme assay is performed and the kinetic data graphed. The y-intercept of a Lineweaver-Burk plot is 0.6 min/ΔA. The substrate being assayed has a molar ...
1
vote
2answers
854 views

What would happen if a person had the right side of their body totally removed (including the brain)? [closed]

What would happen (in reality or theoretically) if the entire right side of a human's body was removed? Could they live (aside from all the probable infections and such)? I would guess that humans ...
0
votes
1answer
38 views

Global Acidification or Warming [closed]

Which poses the greater threat to ecology Global Ocean Acidification or Warming?
3
votes
1answer
121 views

Looking for protocol to produce a drosophila primary cell line

I'm looking for a general (and easy, this is going to be for an undergrad class) protocol to isolate and culture primary cells from fruit flies.
4
votes
1answer
132 views

Main mechanism of the evolution of sexual dimorphism

Sexual dimorphism is very common and (normally) requires the evolution of sex-specific genetic variation/regulation so the genetic correlation between the sexes can be <1. Is there consensus as to ...
1
vote
1answer
205 views

Calories burn rate calculation — scientific studies

There are many online calculators for measuring walking/running/bicycling calories burn rate. They differ in input parameters like some might need my weight as well as height and some don't. Almost ...
1
vote
2answers
38 views

Deduce length order of 4 decapeptides based on the Ramachandran plot

I have stumbled upon the following past exam question: 4 decapeptides A, B, C, D, made only of glycine residues, have different conformations, and the phi and psi angles of individual peptides ...
9
votes
2answers
170 views

Is mammalian vision processed as a sequence of frames?

I often read that people believe that human vision has an inherent frames-per-second rate (FPS) that causes stroboscopic effects - such as seeing the spokes of a rotating wheel apparently rotating at ...
4
votes
3answers
180 views

Western Blot: Fischer ECL substrate 2:1 instead of 1:1?

i recently developed a b-actin-ab-incubated PVDF-WB-Membrane with the Thermo Scientific Pierce ECL Kit. According to the manufacterers orders i mixed Reagent 1 and Reagent 2 1:1. But unfortunately i ...
1
vote
2answers
108 views

what is the modern, molecular explanation of independent assortment of alleles?

in case it isn't obvious i'll begin by stating that i'm a layman, trying to understand something about this subject from reading books. the books i've looked at so far give murky and confusing ...
1
vote
2answers
166 views

Rhizosphere vs. Endorhiza?

In microbiology and the naming of the various areas of the plant as it relates to microbial inhabitance, I am confused as to the difference between the terms endorhiza and rhizosphere. In this case I ...
1
vote
2answers
36 views

Does bioluminescence communcation occur on macroscopic level?

Some bacteria are known to communicate using bioluminescence (quorum sensing). Do we observe the same phenomena in any macroscopic species?
1
vote
5answers
280 views

How much does a typical biologist know about signal processing, machine learning, and (theoretical) computer science? [closed]

How much does a typical biologist know about signal processing, machine learning, and (theoretical) computer science? Coming from a neuroscience background where knowing about algorithms and computer ...
2
votes
2answers
50 views

How much are phase-contrast and dark-field microscopy used in biological research? [closed]

How much are phase-contrast and dark-field microscopy used in biological research today? It seems these were invented a long time ago, so I am thinking would it be a good idea to learn these ...
2
votes
1answer
108 views

How to measure the total number of cells in an average human body?

I have got one assignment to calculate the approximate sum total of all cells in the human body. How to tackle this problem? I know that the current statistics is $10^{13}$ cells. I wanted some hints.
2
votes
0answers
37 views

Subtypes of Acute myeloid leukemia

I am a computer scientist with no biological background and working on analyzing lab results of patients with Acute myeloid leukemia. They have been tagged with following subtypes of AML: AML with ...
25
votes
2answers
2k views

Why was disease transfer to the Americas one-way?

It is well known that the European colonists brought many infectious diseases to the Americas, and that these had a deadly effect on the native populations, because they had no immunity to them. Were ...
1
vote
1answer
82 views

Applying drugs to brain via scalp (transdermal)

Many medications are intended only for the brain, but are taken orally. Hence <10% finds its intended target, while the remaining >90% is at best wasted, and at worst causes unwanted side-effects. ...
2
votes
1answer
248 views

How do I interpret this graph regarding introduced genes and virus-infected cells?

This graph appeared in a practice test for the MCAT. I am trying to interpret it, but it confuses me a bit. On the x-axis we have some introduced genes, and on the y-axis we have % of cells infected. ...
2
votes
1answer
65 views

Sansevieria species specification needed

What are the botanical differences between the species S. parva and S. senegambica? Please highlight the main ones. I have 2 groups of plants. One with label "Sansevieria dooneri" (a synonym of S. ...
1
vote
1answer
269 views

Do alternative start codons code for methionine after transcription?

There is some literature which shows that all start codons code for methionine. However, in the standard genetic code, the alternative start codons clearly code for leucine. Does that mean these ...
7
votes
3answers
770 views

Why has grey hair evolved?

A vast majority of humans get at least some grey hair as they age. As far as I know this applies to both genders and all races. Presumably this means that at least some grey haired humans have ...
2
votes
2answers
83 views

Why isn't Bubonic Plague as virulent as it once was?

Although I am admittedly not anywhere near an expert on any topic, I still have a hard time understanding how Black Death and Bubonic plague are one in the same. Since plague still exists, with news ...
3
votes
1answer
7k views

What is the ratio of breathing rate to heart rate?

Breathing rate and heart rate are directly connected. This is because the heart is connected to the lungs via the pulmonary artery. I first thought that breathing rate would be half of the heart rate ...
6
votes
2answers
182 views

(How) can a pink grasshopper exist?

I saw this foto on Reddit recently: Is this possible? How can a grasshopper become like this? Is this just natural genetic mutation?
4
votes
1answer
114 views

multiple HIV infection in same T cell

I was wondering can multiple HIV virus infecting same T cell ? Coz in flu virus they have SA to cleave of those sialic acid residue preventing re-infection of the same cell by other viruses to ...
1
vote
1answer
170 views

Regarding the difference between cDNA library and RNA sequencing (Biochem. technique)

I was wondering why establishment of cDNA library required the step of reverse transcription (i.e. turning the sequence into DNA) why not directly using the extracted RNA for sequencing and converting ...
2
votes
1answer
160 views

How to estimate the DNA density in human sperm head? [closed]

I have got an estimate of sperm head volume from internet.Like consider it as a disk of order 4-5µm. Now I wanted to find the DNA density in the sperm head. How to find that?
2
votes
1answer
42 views

What triggers the release of gonadotrophin-releasing hormone with the onset of puberty?

I am interested to know the process of development of puberty. During puberty, when boys/girls reache 12-14 (puberty) years of their age, their hypothalamus releases gonadotropin releasing hormone (...
6
votes
1answer
952 views

Alfred Jost's rabbit experiment: how did he actually do it?

I was watching a Crash Course on Biology YouTube video by Hank Green: Great Glands - Your Endocrine System. Hank humorously describes a sex determination experiment by Alfred Jost as follows (...
4
votes
2answers
172 views

Is the water transpired by plants pure H₂O, or does it contain trace minerals?

I'm considering the idea of drinking water that I trap from what is transpired by trees. Interestingly, is this water a fairly (or maybe even extraordinarily) pure form of H₂O, a remarkably pure by-...
3
votes
2answers
97 views

Why do pine stands exist?

I live in the midwestern United States. I cycle to work, sometimes on heavily wooded roads, and I can't help but notice that in most places within the surrounding deciduous forests Pine and evergreen ...
5
votes
1answer
282 views

What is this beetle?

During a pit stop on the road from North Carolina to New York (somewhere in Delaware) I found the following (dead) insect in the car park: As you can see from the quarter next to it (25 mm diameter)...
2
votes
1answer
172 views

What is the name of this big plant with a purple trunk and green to purple berries? [duplicate]

I have photographed the following plant in our garden in Pittsburgh, PA. Do you know the name of the plant?
1
vote
1answer
93 views

How do Type I restriction enzymes work?

According to NCBI, type I REases have a specific target sequence but randomly make cuts at sites far from the target sequence. How does the restriction enzyme travel from the target sequence to the ...
5
votes
1answer
274 views

Is oxidative phosphorylation less efficient in gram positive bacteria?

Gram negative bacteria perform oxidative phosphorylation in their periplasmic regions, between the inner and outer membrane where a proton gradient is maintained and used by the ATP synthase to make ...
4
votes
1answer
570 views

Null distribution of Blomberg's K

I've noticed different functions in different R libraries have different ways of assessing significance for Blomberg's K. For instance, picante's phylosignal looks at a distribution of PIC variance ...
11
votes
1answer
302 views

When did CRISPR/Cas9 evolve and what is the likelihood that a superior system for live cell genome editing has already evolved on earth since then?

I've read that CRISPR/Cas9 is currently being implemented and tested for its ability to edit genomes in live cells, and that it is supplanting other genome editing tools in labs, such as TALENs and ...
5
votes
2answers
370 views

15 30 50 per page