6
votes
2answers
199 views

Ni-NTA purification, problem with the chaperone protein

I'm trying to purify the protein by Ni-NTA affinity chromatography. It seems that my protein (size about 54 kDa) is co-purified with chaperone protein (probably GroEL - as the band is around 57kDa). ...
6
votes
2answers
218 views

How would the human body adjust to sleep times if we were to live in a place with different day lengths?

You sleep at night and are active during the day that's how things work for humans, but theoretically if a human whose parents lived on earth were to be born in another planet resembling earth but the ...
6
votes
3answers
401 views

Does a neuron ever generate an action potential without stimuli?

Most accounts I read involving action potentials and synapses and the like tend to focus mostly on the action potential as a mere automatic reaction to another similar event happening upstream. From ...
6
votes
1answer
1k views

Why don't we have hair on palms?

We have hair all over the body except palms. What is the biological reason behind this? Thanks
6
votes
1answer
8k views

Why can cats interpret things on a monitor/tv, but dogs cannot?

E.g. you can see a lot of videos where cats are smashing some mouse or spider on an ipad, but I have never seen it with a dog. I have a dog myself and I know that he sometimes responds to the sounds ...
6
votes
2answers
167 views

Can any species be bred selectively/engineered to become as diverse looking as dogs?

I've done some research and it appears that dogs are the most diverse looking single species of mammals. The questions that interest me is - are dogs special in respect to genes/gene activation ...
6
votes
2answers
16k views

Can positive and negative blood type of a couple lead to miscarriage?

I want to know can $+ve$ and $-ve$ blood group of a couple could be a cause of miscarriage in pregnancy?
6
votes
1answer
388 views

Is there a normal human melatonin concentration to light level curve?

I'm looking for a graph or a curve that would show the typical concentration of melatonin in blood or saliva for humans over 24 hours. It would be good to know the photoperiod for the same human as ...
6
votes
2answers
175 views

Do omnivore mammals vary food preferences based on dietary needs?

I'm wandering if mammals that can eat many different kinds of food (omnivores) vary their preference for food not only based on the availability, but also based on dietary needs? I'm looking at this ...
6
votes
1answer
197 views

Number of spindle fibres during Metaphase?

During metaphase, the chromosomes are arranged on the equatorial plate and are attached to spindle fibres. After S phase, can the cell be said to attain the configuration of 4n? Also, during ...
6
votes
2answers
7k views

Does mixing alcoholic drinks really make you more drunk?

There is plenty of anecdotal evidence ("beer after wine and you'll feel fine, wine after beer will make you feel queer") that mixing alcoholic drink types leads to a stronger effect, but I can't find ...
6
votes
2answers
204 views

Why are some viruses not communicable person-to-person?

Below are two quotes from the CDC about Hantavirus: "Researchers believe that people may be able to get the virus if they touch something that has been contaminated with rodent urine, ...
6
votes
1answer
448 views

Photosynthetic Pigments vs. Chloroplasts

What is the difference between Photosynthetic Pigments and Chloroplasts? I know plants have both but Prokaryotes (Bacteria and Archeae) only have the former.
6
votes
1answer
162 views

Alternatives to fittest-win and Moran processes as simple mathematical models of selection

When modeling selective sweeps as a micro-building block in models of macroevolution (not to be confused with misuses of this in creationist arguments), I use the fittest-win model of selection as a ...
6
votes
1answer
497 views

Mechanism of Tear Gas (chloropicrin/Trichloro methane)

Tear gas (CCl3NO2) makes people cry, and crying doesn't seem to be a simple procedure. There has to be a triggering process somewhere. Can someone explain its mechanism / how it works?
6
votes
2answers
238 views

Is there such thing as a generic “metabolic type”?

A while ago I picked up a book called "Eat right for your metabolic type". The book discussed the effects of the endocrine system on the overall shape and the speed of metabolism. The book outlined ...
6
votes
2answers
776 views

Are cells really the basic unit of all life? [closed]

Please see comments as to the appropriateness of this question on biology SE. All known life on Earth is made up of cells. It is thus safe to say that all known life is characterized by the presence ...
6
votes
2answers
848 views

For how long and how cold should I perform ethanol/isopropanol precipitations of RNA or DNA?

Precipitating nucleic acids using either isopropanol or ethanol is a very common operation, and I've read some very different protocols on the duration and temperature the precipitation should be ...
6
votes
1answer
679 views

How does water move throughout plants?

I haven't yet found a decent explanation for how water moves throughout plants. It does seem to travel more efficiently upward than out or down. Why is that? How does it travel through the plant?
6
votes
3answers
3k views

Why does so much variation exist within species?

My last phrasing of this question did not go down well, so I will try again. The genotype of species is not always the same. If you ask yourself why not all of these possible expressions except one ...
5
votes
1answer
110 views

Understanding recombination scoring in family pedigrees

I am having some problems understanding recombination, and I am not sure what element I am missing here. This figure is an example from my text book. The pedigree belongs to a family with an autosomal ...
5
votes
2answers
200 views

What is the IC50 exactly?

I am reading the paper "Activity of the bcr-abl kinase inhibitor PD180970" but I don't understand how IC50 works on table 1. Can you tell with simple words and give me an easy example?
5
votes
2answers
193 views

How does the eye know whether to focus further out or nearer in order to bring a blurry object into focus?

If the eye sees an object, but the object is out of focus, the eye could be focusing too near or too far. How does the eye (or the brain) distinguish one situation from the other? How does it know ...
5
votes
2answers
158 views

Problems understanding membrane potential

I understand that membrane potential is the difference of the extracellular and intracellular ionic charges, due to their concentrations. We say that the extracellular space has a charge of 0 and then ...
5
votes
1answer
145 views

Mechanism of Muscle Growth

According to this video (sorry for the poor reference but it represents my level of understanding in physiology), muscle grow as a consequence of repairing micro-lesions. How are these micro-lesions ...
5
votes
2answers
222 views

How is it possible to even hope for an ebola epidemic of this current size to remain contained to a relatively small part of Africa?

As of 2014-09-14 at least 5347 cases of ebola have been identitied. Some estimates are much higher. While experts are sounding the alarm to try to get sufficient resources to contain the outbreak, ...
5
votes
2answers
318 views

Where do the 31 spinal nerves start/meet?

Which part of the brain is the first place (from top to bottom) where all 31 pairs of spinal nerves (on each side) meet? Or if they all start at one place - where is that? Nominally, the beginning of ...
5
votes
1answer
87 views

Scale up of Bacterial growth from 250mL to 1-5L?

I am currently growing up a specific strain of E.Coli with a knockout in 40mL of growth medium (LB) in a 250mL shaker flask... My ultimate goal is to scale up this process to a 1-5L large scale setup ...
5
votes
2answers
110 views

The least complex animals with ability to dream

People can dream, dogs can dream, cats probably too when they are sleeping. What about other creatures? Can a bird dream? Do rats have dreams? Are there any studies about that kind of thing? What ...
5
votes
3answers
283 views

What are some examples of genes that code for multiple proteins?

The title pretty much says it all. It is widely taught that a gene in a eukaryotic system could produce more than one protein due to post-transcriptional modification, but I do not believe I have come ...
5
votes
1answer
280 views

Time spent in phases of cell cycle

I am looking for references to papers containing the time intervals spent in different phases of the cell cycle (ej., G0, G1, S, G2, M for eukaryotes) for different cells. In particular, I am ...
5
votes
1answer
558 views

Do ants within a colony ever fight or come to conflict?

I'm investigating how hierarchical groups of a larger organization with a common goal tend to come to conflict with one another over time. As you decompose a large group (for example a corporation), ...
5
votes
1answer
1k views

Why do organisms excrete nitrogenous wastes?

All plants and animals excrete nitrogenous wastes in the form of urea, uric acid or ammonia. But isn't that a bit of a waste? I mean, there is a shortage of biologically available oxygen in the ...
5
votes
2answers
406 views

What are common causes of unexpected ligation products?

I digested two plasmids, one with EcoRI and AgeI and the other with EcoRI and XmaI. Digests looked as expected, so I purified the respective fragments and set up the T4 DNA ligation (AgeI and XmaI ...
5
votes
3answers
139 views

Finding a template/oligo combination for my first PCR experiment

I'm an information technology engineer. I love biology so I research biological topics and have an interest in PCR. That's why I have decided to create a PCR machine. Everything is done now and I ...
5
votes
1answer
821 views

How do individual plants adapt to changes in temperature?

If you grow a plant from seed in your nice warm house, then plant it out in your temperate garden, it will stop growing for a while, possibly die, and if not, subsequently resume growth. Standard ...
5
votes
1answer
79 views

How does the human liver regrow?

I understand that there would have to be a portion of liver present to regrow from, so how much liver would the minimum to be able to regrow? By what mechanism does regrowth occur?
5
votes
2answers
3k views

What is the function of nodes of Ranvier in axons?

In a neuroscience class I'm taking, it was explained that myelin covers axons in sections, the uncovered sections are called nodes of Ranvier, and signals propagate much faster in the covered ...
5
votes
2answers
362 views

How to find Exons in mRNA Computationally

I'm having trouble finding a method to find exons in the original DNA sequence used to create the mRNA, even given the sequence of the mRNA, as I cannot find a way to reliably identify the beginning ...
5
votes
2answers
1k views

The probability of indirect human fertilization?

What is the probability of conception in situations when sperm isn't directly ejaculated into the woman's vagina, like: The man's or the woman's hands come into contact with sperm, for example when ...
5
votes
2answers
502 views

Survival of E. coli on agar plate at -20 degree C without glycerol stock

If I kept E. coli transformed with plasmid at -20˚C without making glycerol stock, will it survive? Actually I have kept it this way for 18 hours. What will happen with it? Will it grow in fresh ...
5
votes
3answers
2k views

How did butterflies evolve to have eyes on their wings?

Some butterflies, such as the UK native Peacock butterfly (Google Image Search) have markings on their wings that look just like eyes, complete with a white fleck to imitate a convex, transparency ...
5
votes
1answer
192 views

What is an simple way to burn glucose for visible effect?

I want to make a partially working model of the digestive system that could digest complex carbohydrates. My ultimate goal is to be able to cut up some bread, put it into the model, operate it, and ...
5
votes
1answer
69 views

Why are some plants frost tender?

Why do some plants have the ability to stand frost, while others can't? Does it have to do with the size of the water vacuoles in the plant cells?
5
votes
1answer
7k views

How do SDS-PAGE gels differ in a Bis-Tris system vs. a Tris-Glycine system?

Protein migrate differently in Bis-Tris and Tris-Glycine gels. I was curious about the actual reasons why. Do certain gel systems result in a tighter resolution than others?
5
votes
1answer
125 views

What is the distance between the 3' 18s rRNA (the Kozak consensus sequence) and the A-site of eukaryotic ribosomes during protein translation?

Reposted from Quora The Kozak sequence is shown to be important in translation initiation, and the A-site is the anti-codon recognition site. If the distance is not known, how could I find out what ...
5
votes
1answer
610 views

What are the various types of protein-protein interactions

I understand there are a number of protein-protein interactions, but what types of interactions exist? and what are the characteristics of them?
5
votes
1answer
1k views

What is the distinction between F' plasmid and R plasmid?

Is there a difference between an F' plasmid that has taken up a chromosomal gene that conveys antibiotic resistance, and an R plasmid? Is a bacterium containing an R plasmid and yet lacking an F+ ...
4
votes
0answers
36 views

Variance in Fst in the infinite island model

The most famous result in the study of structured populations come from Sewall Wright. He showed that in an island model, where each subpopulation is of size $N$ and the migration rate is $m$, then ...
4
votes
2answers
70 views

Can axons act as receptors?

In all histology books, it is stated that all sensory nerve endings (receptors) consist of dendrites that translate physical stimuli from the environment into neural signals. However, several sensory ...

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