14
votes
5answers
2k views

Why are some genes dominant over others? What is the mechanism behind it?

If I have a brown eye gene which encodes the protein that is responsible for the brown color and have a blue eye gene as well, what is the reason that my eye color is brown? How does one gene maintain ...
8
votes
1answer
607 views

How are antibiotic resistant bacterial infections treated?

For example, how are infections of antibiotic resistant strains of MRSA, Streptococcus, or Gonorrhea treated?
6
votes
1answer
302 views

How do ribosomes contribute to their own synthesis?

In other words, what products synthesized by ribosomes are actual parts of ribosomes (if any)? How are they involved in their own synthesis otherwise? What is the cycle/chain of products starting ...
13
votes
2answers
295 views

Percentage of genome devoted to regulating gene expression

Recently I've been studying the p53 tumor suppressor gene as a model for regulation of gene expression. It's amazing how many different post-translational modifications are known to regulate p53 ...
21
votes
1answer
591 views

Regulation of chromatin structure

Recently, I reviewed the different levels of chromatin structure. The primary level is nucleosomes, where DNA is bound to histones, and has structural similarity to "beads on a string." The secondary ...
8
votes
1answer
603 views

Which human body hormonal systems exhibit 24 hour diurnal cyclical activity?

I'm researching the possible connection between the dream content and the activity of various organ or hormonal systems within the human body. I'm looking for information on biological cycles within ...
5
votes
1answer
235 views

Why dont “growing degree days” have units of degrees Celsius (or do they)?

From Wikipedia: Growing degree days (GDD), also called growing degree units (GDUs), are a heuristic tool in phenology. GDD are a measure of heat accumulation used by horticulturists, gardeners, ...
8
votes
1answer
108 views

Pancretic Acinar Cell - ATP, calcium concentration data

I need to find a decent source of data for concentration of ATP and calcium in the pancreatic acinar cell. So far all I can find is ATP or calcium 'levels' based on fluorescence , which are not ...
11
votes
1answer
3k views

Why can hair grow without limit while eyebrow cannot?

I cut my eyebrow and it grows until reach a certain length. Hair can grow without limit. Why can hair grow without limit while eyebrow cannot?
9
votes
3answers
176 views

How is the sequenced genome of a person useful to him in practice, now?

Currently it is possible and not so expensive for a person to have his genome. This is useful in general for understanding how life works. But, in practice, how is this useful for the specific person ...
7
votes
2answers
69 views

Is there a binding affinity metric for interactions not in equilibria?

I am investigating the strength of binding of a small peptide to a protein by isolating the bound version and subjecting it to collisions with gas molecules (CID mass spectrometry) to dissociate the ...
5
votes
2answers
166 views

How to typeset gene regulatory networks

I have a relatively simply gene regulatory network I would like to visualize, complete with the common arrow and bar symbols used to show, respectively, which genes enhance or repress with other ...
6
votes
2answers
91 views

Why do glial cells of the retina become more abundant the closer they are to the optic nerve?

I am doing a project on expression of GFAP in the zebrafish retina. GFAP is a marker for glial cells. I have found that the glial cells are more abundant the closer they are to the optic nerve at the ...
10
votes
2answers
172 views

What is the least costly method to generate sequential amino acid deletions?

I'm looking to generation sequential deletions from a gene of interest. The total size of this region is 8 amino acids. I'm trying to determine which portion of this region is necessary within the ...
6
votes
1answer
130 views

Ideal low-protein binding membranes

I'm looking through the literature on the topic. It seems like hydrophilic PVDF membranes are ideal for low-protein binding but it also sounds like Regenerated Cellulose is an appropriate ...
9
votes
1answer
123 views

Is bootstrapping an acceptable way to determine standard error for binding constants? [closed]

I'm measuring binding constants my system and I appreciate the usual methods of using replicates to measure standard errors and using those errors to calculate propagation of error. I'm curious if ...
8
votes
1answer
1k views

How does one calculate the resolution of a crystal structure?

Considering that I have a diffraction pattern of a crystal, how would I calculate the resolution of a structure in Angstroms? How does that value differ from the information that I would get from the ...
8
votes
1answer
901 views

How strong is spider silk?

Spider silk is pretty darn strong and all sorts of comparisons are made to steel. I'm more curious about the various moduli of spider silk and how it compares to other materials. What is the Young's ...
6
votes
1answer
351 views

Stabbing muscles when flexed and when relaxed

Just a random question, would a sharp object pierce your muscle more(stabbed with the same amount of force) when the muscle is flexed or when relaxed?
9
votes
1answer
132 views

How would a warm winter affect maple sap production?

The past winter (2011-2012) was warmer than usual. Trees are normally tapped in late February when the daily maximum temperature goes above freezing. However, assuming that there have been ...
17
votes
1answer
553 views

What is the lowest pressure at which plants can survive?

What is the lowest pressure at which plants can survive? How the plants behave in a Martian-type atmosphere? Is there any plant that can survive such atmosphere? Can a lichen grow at Martian ...
13
votes
5answers
4k views

Can any protein be phosphorylated?

I am working with an Arabidopsis mutant with an F-box protein knocked out. It has been shown that F-box proteins targets must first be phosphorylated (Skowrya et al., 1997). I have heard of ...
6
votes
2answers
213 views

PCR amplification and error propagation

Say I have one dsDNA that undergoes normal PCR (where amplification is exponential). If there is a mistake, say a G is swapped for an A during the 2nd round of replication, what percentage of the ...
4
votes
1answer
274 views

What is the molecular mechanism of cystine bond formation?

My bioinorganic and redox chemistry isn't up to par and I'm having a very difficult time understanding the chemical mechanism behind cystine bond formation. Most reactive environments are actually ...
11
votes
3answers
3k views

How long can E. coli stocks be stored at -20°C?

I'm volunteering for a biohacker lab - biocurious in Sunnyvale. The have a pretty good set of equipment - gel boxes, incubators, but they don't have a -80°C freezer yet. I'd like to set up some ...
3
votes
1answer
581 views

Displaying nucleotide at a single position from RNA-seq reads in a BAM file

How do I display a single nucleotide position from reads in a BAM file? I have been looking at variation using samtools mpileup, but I want to actually just display the nucleotide at the position I am ...
9
votes
4answers
4k views

What is the closest species to humans in animal kingdom?

I presumed chimpanzees were the closest relatives of us. However, after watching this TED Talk, it seems bonobos are closer to us both in skeleton and behavioral similarity than chimpanzees. I once ...
6
votes
1answer
180 views

Which cryogenic vials and caps are ideal for storing glycerol stocks?

There are internally threaded, externally threaded, natural caps. For the vials, there are skirts, star-footed, and various bottoms. Why do any of these things matter?
4
votes
3answers
110 views

How to create a collection of anonymous sequences for teaching and testing? [closed]

I am looking for a large collection (>1000) of sequence files (eg. FASTA) from any real organism or a tool to create such a collection. The sequence files would be used for teaching and for testing ...
3
votes
1answer
336 views

Does chicken embryo form the disc called “discoblast” in the cleavage and blastulation of chicken?

This thread is related to my previous thread which is still unsolved mainly. I need to be able to compare chick and human cleavage and blastulation of zygote. My friend says that Chicken have ...
4
votes
2answers
1k views

Is secondary follicle or Graafian follicle arrested in the second metaphase of oogenesis?

My note about which I am not sure: Secondary oocyte is arrested in the metaphase II monthly after puberty until fertilization with sperm. The following picture also satisfies my sentence: . So ...
8
votes
3answers
417 views

Long-term-potentiation and memory. Where do we stand?

I was reading the answers to the question: How and where, in the human brain, are memories stored? and, as expected, LTP and LTD came out. Every time I read about LTP/LTD there is always something ...
8
votes
2answers
370 views

Is it possible to add a new sequence to a blast database without recreating it from scratch?

I have a new sequence and I want to add it to a pre-constructed BLAST database containing other sequences. The makeblastdb help does not give any hint on this regard.
11
votes
4answers
432 views

What can you tell about a person, having only their whole genome as information?

Well, the question is in the title, no explanation need.
7
votes
1answer
7k views

Why does RNA adopt an A-form helix?

RNA is known to form an A-form helix, while DNA generally forms a B-form helix under physiological conditions. From left to right: A-form DNA, B-form DNA, Z-form DNA. Image created by Richard ...
2
votes
2answers
1k views

What are the most important differences between HSP70 and HSP90?

Question originally asked on Quora. These proteins have many functional similarities, so why do cells need both to handle unfolded proteins?
7
votes
1answer
137 views

PDB Mining: Why Do I Find Atoms Less than 1 Angstrom Apart?

I am attempting to find potential Hydrogen bonds between Hydrogen donors and aromatic ring acceptors. I do this by predicting the location of Hydrogens on residues and then calculating how far these ...
6
votes
1answer
704 views

What forms the human amniotic sac?

I am trying to understand the formation of embryonic disc for human and chick so the following question is related to here about formation of embryonic disc. I had thought that the amniotic sac forms ...
7
votes
3answers
734 views

Determining potential protease sites within a recombinant protein

My expressed proteins are frequently truncated and I'm trying to figure out which bands are which. The first thing to come to mind is using PeptideCutter from ExPASy but there is just a data deluge of ...
8
votes
1answer
189 views

Taxonomy for plasmids

I liked the question about F' and R plasmids. What is a good resource that describes what is known about the functions and diversity of plasmids in the wild? Prokaryotes or eukaryotes? Wikipedia ...
6
votes
2answers
1k views

Density of neurons/cells in the mouse brain

Could someone point out some relevant papers or resources (an online DB maybe?) describing the density of neurons (or, more in general, of cells) in different areas of the mouse brain?
8
votes
1answer
260 views

What are the biochemical processes occurring when food spoils?

Let's assume for a minute that microbes themselves and their direct toxic products (i.e. endotoxins) aren't toxic to humans. Let's also discount any innate immune responses the body mounts against the ...
13
votes
3answers
932 views

Why do restriction enzymes tend to have an even number of bases in their recognition site?

When reading my textbook I noticed that in all examples but one from eight the recognition site was an even number of bases. I wondered if this was just a co-incidence, so I took the data from this ...
18
votes
1answer
883 views

ATP cost for gene expression

How would you estimate the number of ATPs required to transcribe, export and translate a single eukariotic protein?
5
votes
3answers
156 views

Can genetically modified genes jump to bacteria in the eater's intestine?

The Guardian ran an article a while back talking about GM gene's jumping to bacteria in an eater's intestine. Has other research confirmed this phenomenon?
13
votes
2answers
578 views

Which is more important for protein expression mRNA structure or codon optimization?

The field seems extremely divided on the debate. On one hand, artificial experiments have suggested that synonymous mutations don't correlate with gene expression but rather, the mRNA 5' structure is ...
5
votes
3answers
2k views

Fetal development, gastrulation and embryonic disc

I am completely confused by the images circulating on the internet of human gastrulation. First, lets see how it happens in deuterostomes. This image depicts the process: (image is from Wikipedia) ...
4
votes
1answer
398 views

What did pangolin scales evolve from?

Were they developed anew or a heritage from fish?
11
votes
2answers
537 views

Antibacterial hand soaps (and related products); what are they good for?

From Wikipedia: Household use of antibacterials in soaps and other products, although not clearly contributing to resistance, is also discouraged (as not being effective at infection control). ...
6
votes
2answers
107 views

Is there a term for tool use in animals?

Is there a technical/scientific term that scientists use to refer to tool making abilities found in certain types of animals? Reference ...

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