8
votes
2answers
567 views

What is the smallest molecule that can present as an antigen to the immune system in the context of allergies?

People often claim, in a colloquial sense, that they are "allergic to everything". Is it possible to have a full-fledged IgE mediated allergic response to very small molecules? I was always under ...
3
votes
1answer
209 views

What molecular processes are involved in pseudopodial extension?

I am curious as to the processes and mechanisms involved in the extension of pseudopodia in amoeba. How does the cell know and control the direction and extent of pseudopodia formation at a molecular ...
2
votes
1answer
290 views

Are there natural selection experiments that quantitatively agree with theoretical modeling?

Has anyone ever conducted an evolution experiment and quantitatively compared it to theory? For example, has anyone ever put bacteria in a million petri dishes filled with antibiotics and calculated ...
6
votes
2answers
249 views

Is local equilibrium a reasonable assumption for evolutionary processes?

Whenever I look at discussions of fitness landscapes (in particular, Kauffman's NK model) the questions tend to resemble: The population is at a local equilibrium, but another equilibrium of ...
-4
votes
1answer
145 views

Were any brain fusion experiments ever conducted? [closed]

Did anybody ever conduct experiments on fusing living brains?
1
vote
1answer
133 views

How to classify equilibrium points [closed]

I have the two differential equations: $$\frac{dN_1}{dt} = N_1(2 - N_1 - 2N_2)$$ $$\frac{dN_2}{dt} = N_2(3 - N_2 - 3N_1).$$ I worked out the equilibrium points to be at $N_1 = 0, \frac{4}{5}$ and ...
7
votes
2answers
300 views

Would muscle fatigue still occur if aerobic conditions for a working muscle is maintained?

Put another way if the muscle is given everything it needs to contract and do work will it ever get tired or have a reduction in energy efficiency? As far as I understand muscles depend upon a ...
7
votes
1answer
763 views

How is the exogenous DNA protected from degradation during bacterial transformation?

During transformation, a bacterium can take up DNA from its environment. A small fraction of bacterial species are known to be naturally competent, meaning that they can engage in this sort of ...
0
votes
1answer
199 views

Contracting muscles in humans

I study biology at school, and unfortunately for me, my program skips the muscles in humans chapter. I know (and mainly, feel) that the movement in one direction isn't created by the same muscle as ...
3
votes
2answers
194 views

What is a bacterial biofilm?

Bacteria produce something called a biofilm. I have found a few definitions; some say it is a complex of live and dead bacteria and others say it is a layer on cell wall. What is it made of? What ...
2
votes
1answer
221 views

What is the energy balance of cooking?

If I cook some food, I get apparently several energetic advantages: thermal : the temperature of the food is closer or superior to the temperature of my body or internal organs, so I do not have to ...
2
votes
1answer
103 views

The oldest common ancesstor of all human beings

Do all human beings have a single oldest ancestor (a man and a woman), or were there were many ancestors at the same time at different places on earth? This article sheds some light. Is there another ...
4
votes
1answer
2k views

What does conditional analysis of a SNP in a GWA study entail?

I am familiar with the use of tag-SNPs in genome-wide association studies to identify gene loci involved in complex traits, but I keep seeing the term "conditional analysis" used without any ...
1
vote
2answers
289 views

Deterministic model of lambda-phage lysis/lysogeny [MATLAB] [closed]

Project 1 Base: Can someone please help me understand what I need from Project 1 Base code to do questions 1 and 2 from the first image? I'm new to MATLAB so a thorough explanation would be ...
2
votes
1answer
927 views

Manipulation of gene expression using VP16 fusion and engrailed fusion to a transcription factor?

Today, a presenter briefly mentioned that gene expression in sea urchins during development might be manipulated using VP16 and engrailed fusions. On a slide, it said that expression might be ...
2
votes
1answer
2k views

What would be the best design for spike-and-recovery and linearity-of-dilution validation experiments in one 96-well ELISA plate?

I have searched in the web for a detailed explanation of doing such validation experiment, but unfortunately couldn't find a satisfactory one. I came across the following sources: Thermo ...
1
vote
1answer
102 views

Reproductive isolation causing evolution

My course book says about reproductive isolation that it does not allow the interbreeding among the individuals of different species. It also says that it is a cause of evolution. How is this cause ...
0
votes
1answer
334 views

Why does the PR form of phytochrome exist?

I know how it gets converted because PR is more stable and when there is neither red or far red light PFR naturally converts to PR. But what is the point of it? If PFR is the biologically active one ...
2
votes
1answer
344 views

What insect is this?

I found it in Russia. Where they usually live? What they eat? Full length is approximately 2 cm.
1
vote
2answers
372 views

How long can you keep sheep eyes in formaldehyde before dissecting?

We're preparing for an experiment in our classroom and my teaching partner and I are at different points in the unit. I am concerned that the experiment will be less successful because of the ...
3
votes
2answers
5k views

Is episome a plasmid or a virus?

A plasmid is a small DNA molecule that is physically separate from, and can replicate independently of, chromosomal DNA within a cell. In general, in eukaryotes, episomes are closed circular DNA ...
5
votes
1answer
136 views

How do omega-3 fatty acids reduce cholesterol at the molecular level?

I have heard that omega-3 fatty acids found in fish are a good way to prevent/reduce cholesterol problems. My question is how do omega-3 fatty acids do that: what are they doing on a chemical level ...
0
votes
1answer
277 views

How the concentration of an antibiotic is chosen for YPD medium?

I need to prepare YPD plates containing hygromycin, but I don't know which concentration I can use. I'll select transformants of Hansenula polymorpha.
10
votes
5answers
17k views

What is the cause of muscle cramps?

According to wikipedia, muscle cramps are caused by myosin fibers not being able to break free from the actin filaments during contraction, resulting in a prolonged contraction. Obviously a lack of ...
2
votes
1answer
73 views

PTMs of proteins via mass spec?

I understand that mass spec is widely used to study PTMs like glycosylation of proteins, but how can mass spec determine correct PTM structure of say glycosylation if two glycan structures have the ...
1
vote
1answer
269 views

What causes peanut allergies?

Surveys show that 0.6 percent of the US population has an allergic reaction to peanuts. This prevalent allergy has even prompted some to ban peanuts from schools altogether. I am completely ...
2
votes
3answers
126 views

What biochemical molecule viewer allows for changes in amino acids and resulting tertiary structure?

I am familiar with the Jmol, Rasmol and PyMoL softwares, and was recently introduced to BioBlender. However, I am completely unaware if any of these programs (or others) are capable of loading a .pdb ...
3
votes
1answer
185 views

What is the translation termination efficiency in mammalian cells?

When I express proteins in bacteria I put at least two stop codons at the end of the gene to increase the termination efficiency. Is this the case in eukaryotic cells too? If I put a single stop codon ...
3
votes
1answer
1k views

Where to put the gene after eukaryotic promoter for best expression levels?

As far as I know there is an optimum distance between a promoter and the gene for the best expression levels. What is that distance for common promoters like CMV, SV40? If you have a first hand ...
0
votes
1answer
41 views

Is there any relation between weight class and extreme lifting performance in O-lifting?

We usually thought that weightlifting is a tough game. It is only for great physique. There are 6 man in history who lifted 3 times their body weight. Check here. 3 of them are Bantamweight(56kg), ...
2
votes
2answers
873 views

Iron deficiency anemia symptoms explanation

In my classes and on the internet, brittle nails and dry hair (or hair loss) are described as symptoms of iron deficiency anemia, but none explains the cause of such symptoms. Does anyone have any ...
2
votes
1answer
1k views

Problems with analysis of small RNAseq data - Adapter trimming

I have always faced a problem while analyzing small RNAseq data, at the step of adapter trimming. Overview of small RNAseq (Illumina) RNA is size fractionated using columns or PAGE 3' and 5' ...
4
votes
1answer
395 views

Can I heat Trizol?

I wonder if I can heat Trizol reagent for 30 min 65C. The goal is to disrupt protein-RNA complex while inhibiting nucleases. (I can't use RNasin cause it's inactivated in 65C, and can't use RVC cause ...
9
votes
2answers
386 views

Chiral (a)symmetry of curly hair (and fur)

Some people have curly hair, as well as fur of some animals can be curly. However, shapes which are curly are not mirror symmetric, much alike screws or chiral molecules. The question is whether ...
3
votes
2answers
168 views

Is it possible to pet/groom birds?

I wonder if it's possible to pet a domestic crow, or an owl. Do birds respond to grooming as cats or dogs, for example? If so, then how does one pet a bird?
2
votes
1answer
94 views

Confusion related to a term probe-by-background interaction

I was reading a paper related to bioinformatics where it uses the drug response on the cancer cells and the gene expression of the individual cells are studied to find any useful insights. Specially, ...
1
vote
1answer
183 views

What's the Evolutionary Purpose of Religion? [closed]

I would imagine the answer to this question would be that religion tends to cause greater compliance of individuals within society, so it is pro-society. Plus these religions tend to incorporate some ...
6
votes
3answers
23k views

Differences Between Protein Motifs and Protein Domains?

I am in a 300-level molecular biology class and am unclear about this concept and how to delineate motifs versus domains of proteins. Any suggestions would be much obliged.
3
votes
3answers
390 views

Is it possible to make bacteria vulnerable to antibiotics it's resistant to?

Since bacteria becomes resistant to antibiotics after an unfinished course, is it possible to do anything to be able to get treatment with the same antibiotic again, and reverse the resistance in the ...
3
votes
1answer
429 views

Perception of distant lights without glasses

I am fairly short-sighted and wear glasses pretty much all the time. Naively, I would expect that when I take my glasses off, the image I see should look very much the same as as a photograph that's ...
4
votes
1answer
169 views

How do the variable portions of antibody genes look in cells which don't produce antibodies?

There are several families of antibodies found in mammals. They may have two or more antibody domains which contain heavy and light chains. The variable regions of the light and heavy chains genes ...
1
vote
1answer
128 views

When is wing growth in the bat complete and how soon can bats fly after wing growth is complete?

I was reading this paper by Cretekos et al (2008), in which they state that wing development in the bat is not complete until after birth - the wing continues to elongate postnatally. This would ...
4
votes
2answers
313 views

How do you store membrane proteins?

We're producing some membrane proteins and they aren't amenable to freeze thaws even when we add glycerol. The proteins are solubilized in detergent above the cmc so they should be in micelle form in ...
3
votes
1answer
515 views

How exactly does marijuana damage brain cells?

I've heard that THC can cause permanent damage to brain cells. I've also heard this reffered to anti drug propaganda. Another theory i've read is that temporary effects reduce intelligence but long ...
6
votes
1answer
103 views

What controls the southern range of willows?

Many willows (Salix spp.) grow in northern states but do not grow in southern states, for example Salix viminalis. What mechanisms control the southern range of temperate hardwoods such as willow? Is ...
1
vote
1answer
432 views

Cyanobacterial photosynthetic oxygen yield

I am somehow not convinced that biotic factors are solely responsible for creating 21% of atmosphere (around 40 million moles of oxygen). There may be additional issues here. Since cyanobacteria ...
1
vote
0answers
57 views

Western blotting of lung samples [duplicate]

I have been working on lung samples to perform western blotting. I have two related questions about this: I get very nonspecific results: at times nothing at all, at times it glows on membrane but ...
3
votes
1answer
202 views

How do axon terminals report to the soma?

It is important to bear in mind that the distance between a neuron's axon terminal and its soma can be extensive, up to about 1m in the human body. The fastest transport along the axon is 400mm/day ...
4
votes
4answers
2k views

How did this spider evolve to mimic exactly a human face and arms?

So I came across something terribly amazing today, that is, a video showing this species of peacock-spider, that literally, transforms into a human face waving his arms about. What could have ...
3
votes
1answer
339 views

What is range of the number of individual organelles in cells

What is the range of the number of individual organelles in a cell? I am not a biologist but I understand that there's one nucleus and sometimes lots of mitochondria, so I am after the total number. ...

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