0
votes
1answer
16 views

Extending a small fragment of DNA

Is there a way to extend a small fragment of DNA, say 150 bp, by making copies of itself and attaching each copy of that small fragment to the end of that 150 bp sequence? For example, I want a 1kbp+ ...
1
vote
1answer
20 views

Is T7 promotor leaky in DH5 alpha?

In my experiment I found that a GFP gene under T7 promotor in high copy number pTAC-2 vector is showing green fluorescence producing colonies of DH5 alpha under UV light. what could be the possible ...
0
votes
1answer
10 views

Methylation Data for Erythrocytes (Red Blood Cells)?

Why is it that white blood cells (leukocytes) are highly characterized in DNA methylation pure cell line profiling, but I can't find any profiles of red blood cells (erythrocytes)? Is blood considered ...
0
votes
0answers
10 views

Nature vs Nurture in Rooster mating dance: Are roosters lame dancers because they never learned any better?

I've seen, read about, or heard several avian courtship dances, flights, songs, and other rituals. They are general very impressive, fancy, and beautiful. Roosters, by comparison, are quite lame. The ...
-2
votes
0answers
13 views

Mitosis Maths Question Chromosomes [on hold]

I just wanted to ask regarding mitosis... if a body cell starts with 24 chromosomes, then how many will the new cells have? Thanks, Bob.
1
vote
0answers
42 views

Can our eyes see faster? [closed]

There are some animals that react faster than normal rate (like flies, etc). I guess it's because they can see faster than other animals and humans, as if time passes slower for them. I think some ...
1
vote
0answers
11 views

can the regeneration mechanism be used in humans that of lizards?

how lizards grow their tail again like is there any mechanism or specific protein which helps them to regrow their tail? can the mechanism of regeneration be used in humans to regenerate our limbs is ...
2
votes
0answers
86 views

Can flow cytometric analysis differentiate between a parent contributing longer chromosomes versus multiple smaller chromosomes?

For flowering plants, can flow cytometry of seed from muti-generational interaction of two species and their offspring help us decide if wide differences in genome size for the two species and their ...
0
votes
0answers
20 views

Twin pregnancy, one natural, one surrogate, is it possible? [on hold]

Looking for any case study or biological reason why the following would / would not be possible: Couple A creates and stores a frozen embryo Couple B gets pregnant naturally Couple B then, without ...
-3
votes
0answers
16 views

After a night of drinking, why am I hungry for information? [on hold]

This is a tough question to phrase appropriately, but I will try my best. Last night, I had a small party at my house, and we ended up drinking a lot of alcohol, both beer and liquor. I was no where ...
-1
votes
0answers
12 views

Evolution of healing for burn injuries in mammals?

Our body had very specialized processes for healing burn injuries. Namely the process of blistering. My question is how this process for healing burns evolved considering how rare burns must be in ...
0
votes
0answers
14 views

Is it better to eat something that is more or less related to humans?

My question is, would it be better to eat something like a sea cucumber or would be it better to eat a chimpanzee? Maybe this isn't a dichotic answer. Is it better to eat something in between, like ...
0
votes
1answer
16 views

What does NaOH do for the plating of Bacillus subtilis?

A book called Molecular Biological Methods for Bacillus called for plating Bacillus subtilis cells on LB-agar plates with the following components: A general-purpose medium containing per litre: ...
-1
votes
1answer
24 views

Monarch mimic other than viceroy? [on hold]

I once saw a butterfly that looked a lot like a monarch. It had orange wings with a black edge and white spots on the edge. It also had 1 black spot in the middle of each wing. I saw this butterfly ...
2
votes
2answers
22 views

Are gizzard muscles voluntary or involuntary?

I started raising chickens this year. They have amazing appetites, and I often wonder what eating is like for them. They have no teeth, so they don't chew their food as they eat it. They do seem to ...
3
votes
0answers
30 views

Did I just watch a spider die?

So I'm sitting in my office and all of a sudden a small spider is gliding down on a web string from the ceiling, right in front of my eyes, and down onto my desk. It proceeds to make a few awkward ...
2
votes
0answers
14 views

transitional mutation, repair mechanism

I have read an answer to a question about dna repair during and after replication. But how is dna repaired if a deamination of of 5-methylcytosine happens? How does a repair mechanism identify which ...
0
votes
1answer
20 views

Is galvinoxyl antioxidant assay possible using NMR spectroscopy?

I would like to perform an antioxidant assay using the galvinoxyl protocol. The protocol states that we need EPR spectroscopy, but only NMR spectroscopy is available at my institution. Is there an ...
1
vote
1answer
21 views

Trial pre-registration service that accepts non-clinical trials, or specifically ecology?

I'm in the early stages of designing a trial in ecology. I'm interested in pre-registering the trial, as suggested here. My concern is that all of the registries (that I can find) deal specifically ...
4
votes
1answer
60 views

How to know the gestation time of pregnant mice?

In lab mice I wish to establish how many weeks my animals are into their pregnancy. Is there some way?
1
vote
1answer
20 views

Ancestral Allele and Hapmap

I notice on dbSNP rs6352 has an ancestral allele of G - http://www.ncbi.nlm.nih.gov/SNP/snp_ref.cgi?rs=rs6352 The hapmap in humans for this allele is very rare, the homozygous version practically not ...
-1
votes
0answers
11 views

Two colors in the eyes [on hold]

my husband has dark brown eyes and they turned blue in his seventies. The brown is back with a blue ring around it. Why is that?
0
votes
0answers
7 views

what is the effect of temperature and carbondioxide on the opening and closing of stomata?

I am teaching biology in an academy. The question which I had asked above had been raised by on of my students. I tried my level best to search out the answer to this question but I could not find the ...
11
votes
1answer
9k views

Why do dogs try to cover their poop?

It may seem obvious to us humans to hide our excreta, but this is not quite normal behavior for animals. Interestingly, dogs have the habit to pee all over the place. It's know that they do so to ...
1
vote
0answers
19 views

Seeing through the rain

This question leads me to wonder about seeing in and through rain. From visual point of view, rain is light-bending droplets moving downwards, unformly in steady rain, less uniformly if there's ...
3
votes
0answers
15 views

Do ginko trees grow well in Eastern North America (specifically Ontario,Canada)

I have recently noticed many Ginko (Ginko biloba) saplings planted in my Eastern Ontario city (in Canada), and they are looking a bit sickly, with yellow leaves. They seem to be a popular choice in ...
-1
votes
1answer
47 views

Do we all dream “every” night? [on hold]

I had an argument with my brother and he claims that he doesn't dream every night ie when he dreams he always remember and if not he hadn't dreamt at all. I'm under the impression that all humans ...
1
vote
1answer
28 views

Rattlesnakes developing stronger venom

I have heard that rattlesnakes are developing stronger venoms causing more people each year to die from rattlesnake bites. But why would a snake that is already very venomous need to develop stronger ...
2
votes
1answer
12 views

What are the differences and similarities between fimbriae and adhesins in microbes adherence factor?

Adherence factors in microbes is to attach to a host cell or to an extracellular matrix. So what is the difference and similarities between fimbriae and adhesins?
3
votes
3answers
115 views

Why does all life use the same macromolecules in their genetic code?

There is no biochemical constraint of any sort, so why doesn't some other code work? Why is it specifically RNA/DNA?
2
votes
2answers
29 views

Are multi-chain proteins synthesized as one biological unit?

Are multi-chain proteins (especially homo/hetero-dimers) synthesized together as one overall unit or are they separate monomers which bind together at some point after synthesis, and are there any ...
0
votes
0answers
5 views

What anaconda species/variants/whatever (if any) would be plausible to keep as pets in Europe? [migrated]

All my life I've had one favorite animal: the anaconda. I used to read up quite a lot of documentation on them, but as time went on, I forgot most of it & haven't really found the time lately to ...
1
vote
1answer
42 views

Possible calculations with temperature, humidity, images and VIS spectrum of a plant in an Integrating sphere

I am currently working on a project which involves growing some plants in a integrating sphere made of foam. I have added temperature, humidity and soil moisture sensors as well as a Spectrometer (350 ...
0
votes
1answer
21 views

Nature of Dengue Fever Genetic Material

Once the genetic material for Dengue Fever is inserted into the human DNA, would the Dengue Fever molecular material be in the form of its own isolated DNA fragment or will it be inserted directly ...
0
votes
0answers
8 views

What is the decision making process when a bird builds a nest?

I have been watching The Life of Birds and I am rather startled by the ingenuity some birds show in building their nests. For instance, one species built a dummy nest on top with a secret entrance ...
2
votes
0answers
20 views

What is the distribution of the number of heterozygotes in finite populations?

Consider a bi-allelic locus with alleles A and a. We denote the frequency of the A allele by ...
2
votes
1answer
440 views

Why do people and animals stretch out their bodies and what is causing this behaviour?

I noticed that my cat which is only 6 months old has started stretching its body from time to time. Then I thought that this motion doesn't seem very natural from another cat's viewpoint, so my cat ...
4
votes
3answers
440 views

Does the genetic material the sperm carries affect its physical properties

Basically, what I'm asking is, is the actual sperm cell built from the blueprint in the DNA of the man or is it itself also a consequence of the DNA it carries? I'd like to know a few more things ...
0
votes
1answer
21 views

Whole Genome Sequencing and B Chromosomes

Do whole genome sequencing techniques detect B chromosomes if such chromosomes are present? My understanding is as follows: How the DNA material in a B Chromosome is mapped depends on the reference ...
4
votes
1answer
4k views

What species of eagle pushes their young to teach them flying?

There are many references to eagle pushing their young off the nest to make them know how to fly, for example this motivational video. It is also described in this website: Let me show you "the ...
1
vote
3answers
31 views

Whole Genome Sequencing and Chromosome Counts

In general, do standard whole genome sequencing techniques rely more on known chromosome counts, independently arrive at chromosome counts, and/or not directly address issues such as base number, ...
0
votes
0answers
29 views

What options are there to consume water and can you survive on them alone?

I thought about this a while, i can think of several ways, but i am not sure if there are other options and if you can survive on them alone withou dying of dehydration (lets ignore other problems ...
2
votes
1answer
22 views

How is pain induced by blunt and sharp objects?

Normally, when something touch you, you won't feel pain. With a great force "touching" you, you will feel pain. However, if something that is spiky makes contact with you, you will feel pain. So, how ...
1
vote
2answers
56 views

What were the symptoms of Phineas Gage after suffering his brain injury?

Phineas Gage was a construction worker who suffered a head injury due to an explosion at a construction site. A metal rod was pushed up his cheek and through his head. I have heard he demonstrated ...
3
votes
1answer
98 views

Do our belly buttons consist of scar tissue?

I read an interesting post on Facebook the other day saying that we all have a visible scar since birth - our belly button. This got me thinking (and research for me only found results regarding belly ...
1
vote
2answers
51 views

How do neurons receive the ions needed for creating electrical pulses?

I really wonder how ions are transported into the brain and the neurons for creating electrical potentials - how do ions get from our digestive system to the neurons? Or are the ions just freely ...
7
votes
3answers
7k views

What's the difference between a plant and an animal?

Are there some living things where the distinction between plant and animal is blurry or unclear? I wouldn't know where to put something like yeast. I also thought of photosynthesis, but then I ...
2
votes
0answers
42 views

How do we know Denisovans had 46 Chromosomes

What allows sequencers to conclude that Denisovans had 46 chromosomes rather than merely knowing Denisovans had the crossover material arranged in 48 or say 44 chromosomes? See ...
0
votes
0answers
37 views

Evolution of a Population

Scientists observe a newly established population of sexually reproducing plants growing on the shore of a small island. An observable trait of the plant has two possible phenotypes. It is determined ...
1
vote
1answer
132 views

Hardy-Weinberg Equilibrium Question

I have four questions concerning H-W Equilibrium: (i) In a population of mice, the presence of black spots is the result of a homozygous recessive condition. If the frequency of the allele for this ...

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