1
vote
2answers
19 views

Why are there seven entries for Pimpinella dioica in the Catalogue of Life?

And six for Haliclona digitata, and five for Ichneumon varius. A couple of the duplicate entries are synonyms for other species, which is understandable, but most are the "accepted name". Shouldn't ...
2
votes
0answers
10 views

Metabolism that generates methanol

I've been looking for help topics about methanol in metabolism. Specifically I wish to know What is a common dietary component that generates methanol following metabolism and comment on its ...
1
vote
1answer
33 views

What is the difference between clinical and non-clinical depression, and is there a term for different severity of the bipolar disorder?

I was looking for a term which describes a bipolar disorder of lesser severity. I know from experience from someone I know well, what a very severe case of the bipolar disorder looks like, when an ...
1
vote
0answers
15 views

Question about mutation of multiple nuclei?

If an organism with multiple nuclei, such as the slime mold Physarum polycephalum, was to be exposed to a mutagen, such as 5-bromouracil, what would be the effect (besides death)? Especially if ...
1
vote
1answer
13 views

PDB file SEQRES have different datas?

In PDB file, SEQRES represent the aminoacids but some pdb file present the "GLC" and some other. for example ...
2
votes
1answer
19 views

How do I prepare a solution? (Calculation) [on hold]

I have to make 100mL of 400mM NaOH. How do I go about calculating what I need to prepare this?
9
votes
1answer
607 views

Why doesn't the cell membrane just… break apart?

Forgive me if this is a silly question. I can't understand the basics. Why doesn't the membrane just... break apart? What's keeping the layers in the phopholipid bilayer together? I know that the ...
1
vote
0answers
7 views

How to automate this custom gene expression microarray chip?

I have 16 gene sequences that I am interested in their detection in clinical samples. I already measured their RNA levels by using RT-qPCR technique and their benefit has already been proven to help ...
0
votes
0answers
19 views

What is Prana?Does flora contain prana? [on hold]

What is Prana?Does flora contain prana? Is this a cosmic energy that is set in earth, nature? Do organisms contain that energy?
1
vote
0answers
28 views

An unknown band appearing in my gel electrophoresis

I am having trouble understanding what is the source of band "2" in the following gel-electrophoresis: In this experiment, we took an E.coli transformant colony and ran its nucleic acids in the ...
3
votes
1answer
19 views

What is the most critical step of plasmid purification?

During the process of plasmid purification and quantification, which step is the most critical to the success of the process?
1
vote
0answers
11 views

fermentation to acetic acid

How many time need for swich fermentation to acetic acid production cycle , and the conversion of apple vinegar in a sealed container incubated at 37 ° C ? Should be in the fully closed? What is ...
2
votes
1answer
68 views

How closely related are two parasites Giardia intestinalis and Trichomonas vaginalis?

How closely related (from the evolutional point of view) are parasites Giardia intestinalis and Trichomonas vaginalis? Giardia genome is about 12 Mb and its major cellular proceses are rather simple. ...
3
votes
0answers
38 views

Can diseases be transmitted wirelessly? [on hold]

According to the recent research about Electromagnetic Signals from Bacterial DNA (A. Widom, J. Swain, Y. N. Srivastava, S. Sivasubramanian) it seems there is a possibility of a "wireless" version of ...
4
votes
2answers
62 views

What is MHC haplotype?

What is MHC haplotype? I did check out the wiki article, but did not understand. (I have not studied biology since last 8 years and now I am going through it because I need it for my research. So if ...
2
votes
0answers
17 views

What is Colloidal biology and does it have any scientific background?

There is the following bulletin published for the History of Chemistry Vol. 32: 105-118 in 2007: “MOLECULAR” VERSUS “COLLOIDAL”: CONTROVERSIES IN BIOLOGY AND BIOCHEMISTRY, 1900–1940* written by PhD ...
3
votes
2answers
48 views

Is it possible to calculate the energy required to speak a word?

I was discussing with a colleague whether speaking would require more energy than communicating via instant message (GTalk, etc..). If this is possible, then I'm particularly interested in whether ...
8
votes
2answers
1k views

Why would taking antibiotics increase stamina and energy?

I often hear that people who are taking antibiotics experience wild fluctuations between feeling full of energy and completely alert but soon after feeling impossibly fatigued and sick. Does this ...
1
vote
0answers
45 views

Question about *Cryptosporidium hominis*

Cryptosporidium muris infects mostly rodents and it is usually found in gastric gladns. Cryptosporidium parvum infects humans and certain animals and it is found in the terminal ileum and colon. ...
1
vote
0answers
13 views

What keeps resting potential of neurons constant at -70mV?

I know the sodium-potassium pump pumps out 3 Na+ ions and pumps in 2 K+ ions per reaction so the negative charge in the axon increases. However, once the voltage (difference of charge inside and ...
1
vote
0answers
8 views

transfection of expression library made from cDNA into cells expressing the desired MHC haplotypes

http://www.sciencedirect.com/science/article/pii/S0167569997800205 If anybody has access to this paper, can you please explain me the process to find TAA based on transfection of expression library ...
6
votes
2answers
683 views

Why do different bacteria have different shapes?

Why do different bacteria have different shapes? Is it only related to their function?
5
votes
1answer
60 views

What is the “Spandrels” debate about?

In a former question, as a side question, I asked a clarification about the "Spandrels" paper. Being not a biologist, it was the first time I encoutered it. Subsequently I tried to collect some ...
5
votes
1answer
14 views

Does gray/white hair turn gray/white from a normal hair? or does a gray/white hair actually grow gray/white originally?

I am really curious if hair actually tuns white/gray from default color, or does it have a defect as it is made and grows out of your head a different color?
3
votes
1answer
19 views

Meta-analysis of fruits in blocking Cytochrome family

My professor says that some drugs cannot be used with grapefruit. I am trying to search a list of cytochromes and their effect to block members in the cytochrome family. Some in Cyt P450. What is ...
1
vote
0answers
46 views

Encephalitozoon cuniculi

Can anyone advise me a literature supporting the claim that Encephalitozoon cuniculi is an aerobic organism? I have seen papers saying otherwise, but also I was told that this is not quite true.
0
votes
0answers
17 views

Data Bias in protein-protein interactions data

Databases like STRING have so many predicted as well as experimental interactions data for many organisms.However there is a bias in the data which they have?. a)The first is gene co-occurrence ...
0
votes
1answer
35 views

How can I download a gene sequence from GenBank (NCBI)? [on hold]

Could you tell me the steps to find and download a gene sequence from GeneBank? I would appreciate your help.
2
votes
2answers
46 views

Why aren't introns found on the ends of pre-RNA?

We recently learned in genetics class that exons always cap the ends of nascent RNA. I have been trying to figure out the reason why introns can't instead be found on the ends instead of exons. The ...
0
votes
0answers
6 views

SRC & RSV virus origins

When a virus infects a cell it undergoes recombination with cell genome. I understand how recombination happens. So in the process, is a gene from virus delivered into human genome or human genome ...
10
votes
4answers
1k views

Where is the line between Anaerobic and Aerobic?

I am well aware of traditional anaerobic respiration (lactic acid or alcohol produced - no Krebs cycle) and traditional aerobic respiration (O2 is used at the end of the Citric acid cycle). I am ...
3
votes
2answers
1k views

Why is Paracetamol so great?

Every time I get ill (cold, flu etc) I take a couple of these wonderful tablets for up to 4 times a day and I, eventually, get better. What exactly is paracetamol? Why is it so effective and is it ...
1
vote
0answers
14 views

Is intron removal coordinated?

Are the introns present in a nascent rna molecule removed simultaneously or are they removed in some sort of sequential fashion or is removal completely random?
1
vote
0answers
14 views

Linkage disequilibrium D sign

Linkage disequilibrium D equals D=x11−p1⋅q1 where: Haplotype > Frequency A1B1 > x11 A1B2 > x12 A2B1 > x21 A2B2 > x22 and Allele Frequency A1 > ...
2
votes
0answers
34 views

Plotting metabolic networks?

I need to draw a map of the core metabolism of E.coli. Associated with each reaction in the map I have a number that indicates the flux through this reaction. I want the map to reflect these fluxes ...
5
votes
2answers
132 views

What is an irreversible reaction?

There are reactions with large Delta G negative values. Why these reactions are irreversible? As in: out of 10 steps of Glycolysis, 3 are irreversible steps. I need an explanation for why they are ...
0
votes
1answer
35 views

Knowledge about Organelles

As we know, there are various organelles present inside a cell. Let's consider mitochondria for example. My questions are -> Do we understand the composition, structural and functional features of ...
0
votes
0answers
9 views

Are there any drawbacks to having a fixed-Köhler microscope?

I am considering to buy the Zeiss Primo star microscope. There are two variants: one with so called "Full Köhler" illumination and one with "Fixed-Köhler" illumination. Are there any drawbacks to ...
-2
votes
1answer
50 views

What is the difference between regular blood, a woman's and a virgin's menstrual blood?

There are many stories that blood contains the life-force energy and specifically menstrual (period) blood has always been a feature of many rituals and some ancient Sumerian tablets mentioned that ...
3
votes
1answer
57 views

Could this Francobelgian comic book woman really have these 4 diseases at the same time?

I'm doing an archive binge of a paper comic "The Kiekeboes". One of the characters is an elderly woman currently at the doctor, and she says the has the following illnesses: Erythema Exsudativum ...
1
vote
0answers
18 views

How to calculate when the velocity declines in an Enzyme Kinetics Problem

If you have an E,S pair that has KM and Vmax values of 2 mM and 1 mM/sec/μM enzyme and you start the reaction with 10 mM [S] and 1 μM [E], approximately how long will the E,S system stay in the ...
-2
votes
1answer
27 views

Affinity isolated peptide

What is affinity isolated peptides??(I have not studied biology since last 8 years and now I am going through it because I need it for my research. So if someone can describe it in simple language it ...
5
votes
1answer
84 views

Help Needed Identifying Corvids

I'd appreciate some help identifying these three corvids I photographed in Austria during the Summer: I know identifying corvids can be difficult, I'm not an ornithologist, just an amateur bird ...
2
votes
2answers
57 views

Manufacturing of Bio Molecules [on hold]

Can we Manufacture bio molecules say e.g. the simplest Sugar, C12,H22,O11 the way we do with any other mechanical component. So, we know all about the Structure and arrangement of Atoms in Sugar - so ...
2
votes
1answer
19 views

Difference between sequential and conformational epitope

Is the difference only in its structure? Like conformational epitope has 3D structure while sequential has a linear structure?(I have not studied biology since last 8 years and now I am going through ...
0
votes
0answers
6 views

Exclusive microtubule -end labeling

Like the title explains I am looking for a way to exclusively label microtubule minus end in vivo. Looking through the literature I could not find any techniques yet. Do you have an idea?
6
votes
2answers
69 views

RNA-Seq library construction challenges: the biases of RNA fragmentation vs cDNA fragmentation

I recently watched a presentation on RNA-seq that covered some of the choices one can make along the way, and I didn't fully understand one of the choices in particular. Near the beginning of the ...
0
votes
0answers
17 views

Oxygen requirements of eukaryotes

Is any of you aware of any resources where I can find information about the oxygen requirement for growth of eukaryotes? As an example, say I'd like to know whether Candida dubliniensis, ...
-2
votes
0answers
16 views

Doubt regarding COBEpro [on hold]

http://www.ncbi.nlm.nih.gov/pubmed/19074155 This link contains the summery of how to predict continuous B-cell epitope. Can someone please explain this to me?? I did read it, but failed to make out ...
4
votes
2answers
312 views

What's the longest transcript known?

What's the longest functional transcript known? I'm wondering about RNA length post splicing, so not including introns.

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