3
votes
1answer
41 views

Looking for protocol to produce a drosophila primary cell line

I'm looking for a general (and easy, this is going to be for an undergrad class) protocol to isolate and culture primary cells from fruit flies.
3
votes
1answer
55 views

Main mechanism of the evolution of sexual dimorphism

Sexual dimorphism is very common and (normally) requires the evolution of sex-specific genetic variation/regulation so the genetic correlation between the sexes can be <1. Is there consensus as to ...
1
vote
1answer
18 views

How to identify the genes that distal enhancers pair?

I am writing a project proposal and I have to talk about this problem: how to identify the genes that distal enhancers pair? I am really new to this topic and I don't know what it is all about. I ...
3
votes
1answer
16 views

How do nicks in the DNA strand affect the success of Long Range PCR?

Long Range PCR using NEB Master Mix - Hot Start Taq was working fine for me (amplicon sizes of ~10kb) but stopped working all of a sudden. Is it possible that many freeze-thaw cycles on the DNA ...
3
votes
2answers
26 views

Is the water transpired by plants pure H₂O, or does it contain trace minerals?

I'm considering the idea of drinking water that I trap from what is transpired by trees. Interestingly, is this water a fairly (or maybe even extraordinarily) pure form of H₂O, a remarkably pure ...
-2
votes
1answer
29 views

What prevents this blue lobster from becoming dominant?

Like everyone else in the world I saw the blue crustacean from becoming dominant? I would think the blue camo would be an evolution advantage.
2
votes
1answer
25 views

Enzyme Assay, absorbance change to concentration change

I have a few issues with this question. An enzyme assay is performed and the kinetic data graphed. The y-intercept of a Lineweaver-Burk plot is 0.6 min/ΔA. The substrate being assayed has a molar ...
2
votes
0answers
17 views

What should be the distance between plant promoter and gene?

I am trying to clone a rice gene under a different endogenous rice promoter.I will be cloning the CDS of the rice gene.So I wanted to know what is the minimum or maximum distance I should put between ...
6
votes
1answer
50 views

What are the effects of sleep deprivation?

Having recently pulled an "all-nighter" while trying to fix a fatal bug in our release code, I started thinking of any health risks that I might have. What are the adverse effects of staying up for ...
0
votes
1answer
35 views

Global Acidification or Warming [on hold]

Which poses the greater threat to ecology Global Ocean Acidification or Warming?
1
vote
2answers
39 views

what is the modern, molecular explanation of independent assortment of alleles?

in case it isn't obvious i'll begin by stating that i'm a layman, trying to understand something about this subject from reading books. the books i've looked at so far give murky and confusing ...
1
vote
2answers
22 views

Deduce length order of 4 decapeptides based on the Ramachandran plot

I have stumbled upon the following past exam question: 4 decapeptides A, B, C, D, made only of glycine residues, have different conformations, and the phi and psi angles of individual peptides ...
4
votes
3answers
134 views

What is the difference between different brain regions

The brain is separated into different regions, and different regions perform different tasks. Well, what are the differences between these regions on the cellular/systemic level. The brain is made up ...
1
vote
1answer
20 views

How long does it take for commercial fertilizers to break down? [on hold]

Sometimes we buy carrots that are abit bitter, it's the taste of fertilzer. Once i put a tomato in the fridge, and when i ate it, it was bitter like shweppes, and i figured that was fertilizer also. ...
7
votes
2answers
46 views

Is mammalian vision processed as a sequence of frames?

I often read that people believe that human vision has an inherent frames-per-second rate (FPS) that causes stroboscopic effects - such as seeing the spokes of a rotating wheel apparently rotating at ...
1
vote
1answer
17 views

Calories burn rate calculation — scientific studies

There are many online calculators for measuring walking/running/bicycling calories burn rate. They differ in input parameters like some might need my weight as well as height and some don't. Almost ...
3
votes
2answers
102 views

What are the factors that control the speed of propagation of neuronal signals?

If we consider an analogy between a wire and a neuron there may be some resemblance between the factors controlling the data flow rate. For example the increased width of wire leads to decreased ...
4
votes
1answer
38 views

Can the negative afterimage appear only if there is light or is it possible in darkness?

Reading the following paragraph: After staring at the red and blue shamrock, you saw a green and yellow afterimage. Opponent-process theory proposes that as you stared at the red and blue ...
6
votes
2answers
38 views

Neuronal Architecture of the Brain

To what extent do brains (e.g. of humans) contain recurrent connections? I am studying artificial neuronal networks and frequently encountered the statement, that recurrent neural networks are ...
1
vote
2answers
22 views

Does bioluminescence communcation occur on macroscopic level?

Some bacteria are known to communicate using bioluminescence (quorum sensing). Do we observe the same phenomena in any macroscopic species?
2
votes
2answers
30 views

Western Blot: Fischer ECL substrate 2:1 instead of 1:1?

i recently developed a b-actin-ab-incubated PVDF-WB-Membrane with the Thermo Scientific Pierce ECL Kit. According to the manufacterers orders i mixed Reagent 1 and Reagent 2 1:1. But unfortunately i ...
0
votes
1answer
10 views

Rhizosphere vs. Endorhiza?

In microbiology and the naming of the various areas of the plant as it relates to microbial inhabitance, I am confused as to the difference between the terms endorhiza and rhizosphere. In this case I ...
1
vote
2answers
25 views

How much are phase-contrast and dark-field microscopy used in biological research? [on hold]

How much are phase-contrast and dark-field microscopy used in biological research today? It seems these were invented a long time ago, so I am thinking would it be a good idea to learn these ...
6
votes
3answers
175 views

Can genes change as we age?

Let's say you're a 23 year old man who impregnates a woman. Will your genes be the same if you were to impregnate another woman at age 35? Will your genes in those 12 years have ...
0
votes
5answers
64 views

How much does a typical biologist know about signal processing, machine learning, and (theoretical) computer science? [closed]

How much does a typical biologist know about signal processing, machine learning, and (theoretical) computer science? Coming from a neuroscience background where knowing about algorithms and computer ...
2
votes
3answers
1k views

What if rB=C in hamilton's rule?

Hamilton's rule states that if rB>C then a gene giving altruistic behaviour will increase in frequency in the population. What would happen if rB=C? Will an individual perform the altruistic act?
2
votes
2answers
201 views

Why is the strength of genetic drift inversely proportional to the population size?

I saw a concept on the Internet that says "the strength of genetic drift is inversely proportional to the population size". I don't know why they are inversely proportional? Can somebody explain? ...
6
votes
4answers
195 views

What prevents predator overpopulation?

I've often heard that a population, human or otherwise, will continue to grow as long as there is food available (assuming nothing else is killing them off). It makes sense: if you have food you can ...
2
votes
2answers
1k views

Why aren't animals diverse in phenotype? [duplicate]

I am not comparing a cat with leopard. I am just saying that we humans are all one type of creature and we are diverse (I am not saying we are class of mammals and phylum of etc and kingdom etc, ...
1
vote
0answers
15 views

Given a microscopic image of a plant, is it possible to determine its age?

Given a microscopic image (let's assume a standard brightfield image) of a plant, is it possible to determine its age?
2
votes
1answer
24 views

multiple HIV infection in same T cell

I was wondering can multiple HIV virus infecting same T cell ? Coz in flu virus they have SA to cleave of those sialic acid residue preventing re-infection of the same cell by other viruses to ...
1
vote
1answer
27 views

How to measure the total number of cells in an average human body?

I have got one assignment to calculate the approximate sum total of all cells in the human body. How to tackle this problem? I know that the current statistics is $10^{13}$ cells. I wanted some hints. ...
2
votes
1answer
36 views

Definition of Linkage Desiquilibrium (LD)

According to wiki, linkage disequilibrium $D$ equals $$D = x_{11} - p_1\cdot q_1$$ where: $$ \begin{matrix} \text{Haplotype} & \text{Frequency}\\ A_1B_1 & x_{11}\\ ...
5
votes
1answer
76 views

Where can I find the pMON7124 plasmid sequence?

I need the map of the pMON7124 plasmid. As this is not easy to find, I welcome any hints.
3
votes
1answer
3k views

Why is my faeces black in color after eating Oreos

Why is my faeces black in colour the morning after I eat some Oreos? Day 1 : Eat a handful of Oreos & the next morning your stool is black. Day 3 : Eat a handful of cocoa flavored biscuits & ...
5
votes
2answers
59 views

(How) can a pink grasshopper exist?

I saw this foto on Reddit recently: Is this possible? How can a grasshopper become like this? Is this just natural genetic mutation?
2
votes
0answers
18 views

Subtypes of Acute myeloid leukemia

I am a computer scientist with no biological background and working on analyzing lab results of patients with Acute myeloid leukemia. They have been tagged with following subtypes of AML: AML with ...
4
votes
1answer
27 views

Null distribution of Blomberg's K

I've noticed different functions in different R libraries have different ways of assessing significance for Blomberg's K. For instance, picante's phylosignal looks at a distribution of PIC variance ...
5
votes
3answers
2k views

What are the advantages and disadvantages of using beta-galactosidase compared to luciferase as a reporter gene?

In the University labs, we have used Beta-galactosidase as a reporter gene to quantify the expression initiated by the stress-response promoter in yeast. This was done by exposing one of the two ...
15
votes
2answers
2k views

Why was disease transfer to the Americas one-way?

It is well known that the European colonists brought many infectious diseases to the Americas, and that these had a deadly effect on the native populations, because they had no immunity to them. Were ...
1
vote
1answer
32 views

Applying drugs to brain via scalp (transdermal)

Many medications are intended only for the brain, but are taken orally. Hence <10% finds its intended target, while the remaining >90% is at best wasted, and at worst causes unwanted side-effects. ...
6
votes
0answers
61 views

How does Sodium Valproate cause neural plasticity

I have been reading a fascinating paper: Valproate reopens critical-period learning of absolute pitch 18 individuals were given Sodium Valproate (VPA) for a fortnight during which they trained on a ...
1
vote
1answer
36 views

How do I interpret this graph regarding introduced genes and virus-infected cells?

This graph appeared in a practice test for the MCAT. I am trying to interpret it, but it confuses me a bit. On the x-axis we have some introduced genes, and on the y-axis we have % of cells ...
1
vote
1answer
31 views

Do alternative start codons code for methionine after transcription?

There is some literature which shows that all start codons code for methionine. However, in the standard genetic code, the alternative start codons clearly code for leucine. Does that mean these ...
2
votes
1answer
24 views

Sansevieria species specification needed

What are the botanical differences between the species S. parva and S. senegambica? Please highlight the main ones. I have 2 groups of plants. One with label "Sansevieria dooneri" (a synonym of S. ...
1
vote
0answers
19 views

How is a tanglegram calculated?

In this question, it was asked what are some good softwares for calculating tanglegrams. What is a tanglegram? How is it calculated? It looks like some kind of double-dendrogram. Do I get a Nature ...
1
vote
0answers
31 views

How to stop or slow down the process of growth of hairs? [closed]

Is it possible to slow down or stop hair growth? If yes, then how is it achieved?
1
vote
2answers
72 views

What is the reproductitive advantage of grey-haired humans

A vast majority of humans get at least some grey hair as they age. AFAIK this applies to both genders and all races. Presumably this means that at least some grey haired humans have noticeable ...
3
votes
1answer
31 views

What is the ratio of breathing rate to heart rate?

Breathing rate and heart rate are directly connected. This is because the heart is connected to the lungs via the pulmonary artery. I first thought that breathing rate would be half of the heart rate ...
6
votes
1answer
35 views

Alfred Jost's rabbit experiment: how did he actually do it?

I was watching a Crash Course on Biology YouTube video by Hank Green: Great Glands - Your Endocrine System. Hank humorously describes a sex determination experiment by Alfred Jost as follows ...

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