-1
votes
0answers
10 views

Is there a way to convert/export track files (.trc file) generated from Media Cybernetics Image Pro Plus to .mat file type? [on hold]

I am trying to export these files so that they can be used with a matlab based automated behavior classification software JAABA. At the very least I would like to figure out a way to read the format ...
0
votes
0answers
13 views

How is the acetone method different from QB buffer for extraction of plant protein?

I primarily work on animal tissue but I thought of running an SDS-PAGE for plant tissue just for fun. I searched for some protein extraction protocols online and I don't know which one to choose. One ...
0
votes
2answers
35 views

Spatial dimensions for an animal

I'm reading a review paper. They say: The position of an object in head-centered coordinates (that is, relative to an animal's head) can be defined along three axes: the medio-lateral (radial) ...
4
votes
2answers
80 views

Does a plant have to have leaves to produce fruit?

Is it possible that a plant can still produce fruit, even though it may not have leaves? Are there any plant species that can produce fruit without leaves?
-1
votes
0answers
14 views

How can I grow seedless strawberries [on hold]

Many people with diverticulitis are under the impression that they can't eat foods with seeds. Although this does not seem to research based the practice seems to persist. I want to know how I can ...
3
votes
0answers
51 views

Why did we evolve to control our breath?

I had this question in my mind and tried to think of why we would evolve to be able to control our breath. I remembered the Aquatic Ape Hypothesis, which makes a lot of sense and would explain it, ...
0
votes
0answers
32 views

Deletion of codons at the start of a sequence in preparation for heterogeneous expression. Why?

I am reading a patent where they isolate a gene from cDNA constructed from RNA extracted from plant matter. The subsequent step (in preparation for heterogeneous expression in E Coli.) puzzles me: ...
7
votes
1answer
72 views
+50

What makes worms' eggs float or sink?

In parasitology, while examining the stool sample, one important thing you do is test whether the eggs float on water or sink. We never did that since our coursework limits itself to microscopic ...
-3
votes
1answer
38 views

Can you naturally change your DNA that's passed to your offsprings? [on hold]

Is it possible to change the genes that you'll pass on to your offsprings by natural activity and behavior. For example if you do body building for quite a long time build strong muscles then would ...
3
votes
1answer
99 views

Glycerol 3-phosphate nomenclature

Why is Glycerol 3-phosphate named in such way? Shouldn't it be named as Glycerol 1-phosphate by proper IUPAC- nomenclature?
1
vote
1answer
68 views

True or False: Dideoxynucleotide sequence analysis Question

True or false: Dideoxynucleotide sequence analysis is a template-directed method that makes use of chain terminators that stop DNA synthesis because they lack a 2'OH group. The answer is false. ...
0
votes
1answer
275 views

0.1 M sodium citrate in 10% ethanol and DNA solubility

I understand how DNA precipitation works in the presence of salt (such as 0.3 M sodium acetate or 0.2 M sodium chloride) and alcohol (30 ~ 50 % isopropanol or 60 ~ 75% ethanol) However, in the Trizol ...
15
votes
2answers
145 views

What are the main mechanisms of interaction between the nervous and immune systems?

We know from pop science that our psychological states have an effect on our immune systems ("worrying ourselves sick", etc.), but what are the actual mechanisms through which our nervous systems pass ...
0
votes
0answers
5 views

Feedback mechanisms for the dynorphin-kappa opioid system?

(this is all happening in the brain). Also it's best to go context-first I think (1) Normally if you take an agonist drug that hits a receptor "x", eventually the brain will downregulate the density ...
5
votes
1answer
47 views

Is this a firefly?

It glows without interruption. This photo was taken near Barcelona, Spain. Thanks in advance.
3
votes
1answer
24 views

Japanese Crab Identification?

I saw a crab walking home from work the other night. We're by a river, but I was probably 500 feet away from the river when I saw this one. I apologize for the image quality, all I had was my terrible ...
7
votes
0answers
103 views

Identification of a mammal in cage

This unidentified animal was trapped in northern part of Kerala State India. Following loss of domestic fowls and suspecting toddy cats a trap was placed and this unidentified animal was trapped. No ...
0
votes
1answer
32 views

How well do Eppendorf cups seal?

I am considering to send some samples overseas in Eppendorf cups. They are the standard plastic cups of 1 to 2 mL capacity. Of course they may tip over during their journey and I'd like to know how ...
1
vote
0answers
36 views

Why is sex pleasure giving activity but not childbirth?

Asking from an evolutionary viewpoint. Sex is a pleasure giving activity so that it encourages reproduction and spreading of genes. But childbirth ,the most crucial part of the reproductive process, ...
5
votes
2answers
113 views

Is it possible to have different genes in different parts of our body?

I want to understand genetic mutation specially in the context of multicellular organisms like humans. I studied biology only till high school and I can’t fully understand wikipedia pages on this ...
4
votes
1answer
76 views

Can the human body create glucose out of fat?

I read conflicting views about whether or not the human body can create glucose out of fat. Can it?
3
votes
3answers
46 views

Needleman Algorithm for Optimal Alignment of two Amino Acid Sequences

I want to compute the optimal alignment of two amino acid sequences as per the following definition from a patent: "The percentage of identity between two peptidic or nucleotidic sequences is a ...
0
votes
0answers
21 views

Insect behavior - why do they land on my window and refuse to move [on hold]

Why do so many insects, particularly bees, land on my window screen and refuse to move even if I hit the window or the screen to make it go away. They do this to my car window too. I see no reason for ...
0
votes
0answers
14 views

Why can dogs not have a pink(ish) coat color?

In yellow dogs, there is a mutation in the MC1R gene which leads to a nonfunctional receptor. This leads to the production of pheomelanin which is pink/red (depending on concentration) instead of ...
-1
votes
0answers
9 views

What's the difference between “mixing” and “combination” of chromosomes? [on hold]

What's the difference between "mixing of chromosomes" and "combination of chromosomes" ?
1
vote
1answer
25 views

Ways to monitor enzyme kinetics with very fast time resolution?

I'm interested in any way to do time-resolved study of enzyme kinetics. I am studying some physical variables that may affect kinetics, but I want to study how quickly they take effect, and how long ...
21
votes
1answer
3k views

Why do mints make your mouth feel cold?

Why do mints make your mouth feel cold? And specifically, why does your mouth feel so cold if you eat a few mints and then drink a glass of cold water afterwards?
4
votes
2answers
68 views

Do any terrestrial herbivores use auditory crypsis for predator avoidance? [on hold]

Some terrestrial predators "stalk" their prey: they sneak up on it slowly, maintaining a low profile, while keeping as close to silent as possible. This makes sense from an evolutionary perspective: ...
17
votes
4answers
2k views

Book recommendation on population/evolutionary genetics?

I have recently been involved in collaborations that require me to model the population genetics of eukaryotic populations. I fear I may either be "re-inventing the wheel" or making conceptual ...
0
votes
0answers
12 views

Is there any technique other than restriction enzymes to introduce staggered cuts into DNA?

I'm curious about introducing staggered cuts into DNA, but wonder if there is a way to do this in vivo in E. coli, as I heard introducing restriction enzymes on a plasmid would end up just shredding ...
7
votes
4answers
13k views

Why do men have a higher hematocrit (red blood cell count) than women?

The hematocrit, also known as packed cell volume (PCV) or erythrocyte volume fraction (EVF), is the volume percentage (%) of red blood cells in blood. It is normally 45% for men and 40% for women. so,...
4
votes
1answer
41 views

Cancer biology: Can tumors form from cells that are genetically identical to non-cancerous cells?

Can a tumor begin to form without any genetic mutations? I'm specifically interested in a tumor which could later lead to cancer.
0
votes
0answers
19 views

Is there good evidence to justify drinking vitamin supplements for health benefits? [on hold]

I stumbled upon this question when trying to decide if I should keep drinking fish oil pills. Technical papers in biology are outside my scope, though, and I don't trust mainstream online articles ...
-1
votes
0answers
23 views

When frozen rain moves up and down within an anvil cloud, _____ is formed [on hold]

When frozen rain moves up and down within an anvil cloud, _____ is formed. A. hail B. snow C. thunder D. lightning
1
vote
2answers
108 views

Why aren't there any transitional animals today?

You have probably heard this question before and in different formats. Usually, it is used as a "proof" to disprove the theory of evolution. I understand that the apes we descended from are not the ...
2
votes
1answer
33 views

Why do blue jays eat my cat food?

We buy some run of the mill big-box store cat food. Over the last several weeks, I've observed a blue jay landing on the cat food dish, taking a piece or two, and flying off with it. I assumed it was ...
3
votes
2answers
113 views

How do small birds survive the winter?

I live in a region with cold winters (ca. -40 C) and many quite small birds (sparrows, crows, ducks) stay for the winter. How can the birds survive this? As I understand it, smaller bodies will be ...
1
vote
1answer
47 views

Is QIAPI 1 a scam?

I find it strange that there isn't a wikipedia article on a drug that allegedly modulates human photosynthesis. I thought I might get a virus from some of the sites selling it, but others seemed ...
-3
votes
0answers
37 views

Is it possible to create the “perfect plant” at home? [on hold]

I am starting a hydroponics farm/garden and I am interested in creating something like the perfect lettuce. I want attributes like crunch factor and shelf life. Is this a possibility that I could do ...
-2
votes
0answers
18 views

How much level should I overexpress a protein without any diseases as references [on hold]

I am planning to overexpress this G protein alpha subunit by using adenovirus in HUVECs. Problem is, currently, no report showed that this protein expression is increased in any diseases. The only ...
1
vote
0answers
16 views

How do you technically perform the Ames test?

Is it necessary to take into account the volume of gm-agar(bottom) in the concentration determination, or is that determined only by the concentration in the top agar? I found several articles that ...
1
vote
1answer
48 views

Correlation of non-coding DNA with coding DNA

I sequenced the first exon of the MC1R gene of 15 labradors (genomic DNA) to look for the loss-of-function mutation (C.916C>T) and as expected, it was where it should have been (916th base pair). As i ...
1
vote
1answer
25 views

Can restriction sites be used as PCR primers?

I have a DNA fragment that contains the gene of interest sandwiched by restriction enzyme sites. Then, can PCR amplify the entire fragment, recognizing the restriction enzyme sites?
1
vote
1answer
67 views

Taste of salt water

I had a question relating to chemistry and biology, so please tell me if this is suited better for the Chemistry website. My question was, if water dissolves salt by separating the two ions, the Na+ ...
4
votes
1answer
41 views

Constantly wiggling moth pupa - will it emerge soon?

Today I found a moth pupa in the soil in my garden in western Sweden. It's about 15 mm long. I have found similar ones before, but this one is wiggling a lot more, even after I put it down and put a ...
3
votes
2answers
63 views

Computing Percent Identity between DNA / Amino Acid Sequence

What are some tools where I can input a pair of DNA sequences (or alternatively a pair of Amino Acid Sequences) and compute a percent similarity identity metric between them? Is BLAST the right ...
2
votes
1answer
125 views

How to interpret this cuffcompare result? Sensitivity over 100?

I run a cuffcompare: cuffcompare -r transcripts.gtf TransStandard_1.0.gtf I got the following result: ...

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