-2
votes
0answers
31 views

PCR, writing methods section for a journal, I am quite inexperienced in writing, and was just checking in if this is okay [closed]

Touchdown PCR was performed for site directed mutagenesis in a 25ul reaction volume of 2μl of template pET15B in combining 16μl of Nuclease –free deionised with 2.5μl of 10X Standard Taq Reaction ...
3
votes
1answer
22 views

Estimating RPM to RCF in Methods from Older Papers

I'm attempting to replicate a cell biology method from a 1958 Laboratory Investigation paper. The protocol is for the isolation of an extracellular matrix protein, and a key step is a centrifugation ...
3
votes
1answer
170 views

Bug Identification: Is this small tubular, black bug a bed bug?

So I found this tiny bug on my wall in the bedroom of my apartment. I estimate it is about 3mm long by 1mm wide. Is it a bed bug? It does not seem to match any of the pictures I've seen online. ...
0
votes
0answers
25 views

Maximum heart rate increase/decrease while physically active [duplicate]

I'm a computer scientist and implementing a heart-rate monitor for physical exercise. While I try to improve my code, I stumbled over the question how fast the heart rate can change within a given ...
6
votes
1answer
129 views

How are bones growing, if bones are not connected to the brain?

If the bones are not connected to the brain, how is their growth controlled? This question is not a duplicate of the question Mechanisms of bone growth, as this question deals with how bone growth is ...
0
votes
0answers
19 views

All cells in an embryo have the same heredity, yet they develop into different tissues and organs and organ systems. [closed]

To Remi.b All cells in an embryo have the same heredity, yet they develop into different tissues and organs and organ systems. What is the process by which this happens? THANKS :) (is it a proper ...
1
vote
0answers
26 views

FoxO gene(s) and aging [closed]

How does the FoxO gene affect aging? I read about how Hydra uses the FoxO gene to live (practically) forever... How does FoxO gene work? Currently, all I know is FoxO regulates the stem cell ...
1
vote
0answers
18 views

What ideas have been proposed about what the first cell would have been like? [closed]

Have any researchers proposed a potential first cell (what it looks like, how it functions), and what do/would they base it on? Ubiquity of life, available ingredients? Also, in the same way protein ...
3
votes
1answer
37 views

Why do small fonts strain the eyes?

I understand that too much close reading will strain the ciliary muscles of the eyes. But what about small fonts? The contraction of the ciliary muscles is dependent on the distance not font size. In ...
5
votes
3answers
91 views

Studying effects of alcohol on cells

I am wondering about the logistics of a simple experiment with say 3 types of alcohols, and various concentrations of each, with a control(s) I would like to research the effects of alcohol on ...
3
votes
0answers
22 views

Why are most transcription factors enhancing the expression rather than repressing?

One can classify the effects of Transcription Factors (TF) on gene expression into two types: it either enhance or repress the gene expression. I have always been told that most of transcription ...
0
votes
0answers
16 views

How can site-directed mutagenesis be used to suppress the production of anti-antibodies?

In a previous post of mine, I asked how to supress the creation of anti-antibodies in vivo. In the answer, it was mentioned that site-directed mutagenesis could be used. Currently, I can't find mcuh ...
3
votes
0answers
22 views

Well to well variation in thermal cycler fluorescence?

We have an old BioRad ICycler Thermal Cycler with MyIQ single color fluorescent detector. While it's meant for RT-PCR, we've been using it for melt curves and binding assays for different types of RNA ...
-2
votes
0answers
83 views

Why do pregnant females still get horny? [closed]

I thought this was a good place to ask. Remembering back to when my wife was pregnant (we were both teens), I recall that she would not keep her ... hands off of me in some times. It will like she ...
1
vote
0answers
20 views

how to store the genome bam file on hadoop [closed]

How to store the bam file which has chromosome data on hadoop ? So that the edge should process chromosome wise without splitting the data
0
votes
0answers
28 views

site directed mutagenesis [on hold]

I have read about site directed mutagenesis (a method used to edit genes and DNA) and had some questions about it. How is site-directed mutagenesis done? I know they are a couple methods so what are ...
6
votes
1answer
52 views

Number of transcription factor genes in the human genome

What is the number of the transcription factor genes present in the human genome? Does this value differ compared to Mus musculus, Drosophila melanogaster, Arabidopsis thaliana, C. elegans and S. ...
3
votes
1answer
59 views

How to attract spiders to my house?

I've been living in my apartment for a year now, and noticed something lately. During spring and summer I have quite a lot of midges indoors, and even now there are some. But there are no spiders! So ...
2
votes
0answers
12 views

Resource for finding Repressive/Inhibitory factors for a given gene?

I have a list of genes for each of which I'd like to find: A list of transcription factors that up-regulate the gene A list of inhibitory factors that down-regulate it. I used this tool on ...
-1
votes
1answer
38 views

Effect of Western chicken and GH [closed]

I heard this clause from my teacher one week ago: Chicken are grown in half a year until eaten in Baltic countries - Estonia, Latvia and Lithuania, for instance, [probably in other parts of ...
1
vote
0answers
26 views

How does a bacterial colony, grown in a nutrient medium, change the chemical composition of that medium over time? [closed]

In order to culture cells, the nutrient medium they are grown in must contain certain chemicals and trace elements (e.g. Hams medium, for culturing human cells, and a less demanding solution for ...
0
votes
1answer
51 views

Can swallowed fingermails, hair, or skin get caught in your appendix? [closed]

There is a common saying in my place: If you eat your skin, hair or nails, it will be deposited in the cecal (Vermiform) appendix, and can cause appendicitis. (This is mostly told to children to ...
5
votes
2answers
81 views

Why deoxyribose for DNA and ribose for RNA?

Why is DNA made out of deoxyribose and RNA made of ribose? Why can't they both use ribose or deoxyribose? I think that the deoxyribose gives an advantage in storing genes, the job of DNA and ribose is ...
8
votes
1answer
109 views

Why don't McDonald's fries decompose?

So I was cleaning out my car and found a McDonalds French fry. as I don't eat anything in my car I know exactly who and when this fry is a result of. The when is + 10 months old and it could pass off ...
5
votes
1answer
62 views

Why does DNA have its name?

Why is DNA called deoxyribonucleic acid and not something else? I get the nucleic acid part (because that's what DNA is made of) but what about the deoxyribo- part, especially the ribo- part. Maybe ...
0
votes
0answers
15 views

Would biotechnology in agriculture actually decrease biodiversity, as many people say? Does it have to?

I've heard the claim that because farmers would be using the same seed, you would end up with an entire crop population that could be susceptible to a disease. Like if you had a diverse population of ...
2
votes
3answers
93 views

fastq-dump using mkfifo to stream data

So, fastq-dump has the ability to be run on just an SRA file accession number, such that the SRA is converted to FASTQ on-the-fly, and the SRA doesn't have to be written to disk. I'm curious whether ...
2
votes
1answer
29 views

Western blot alternatives

Given an in vivo sample from an experimental infection, I would like to see if a bacterial protein is present. I've been thinking about Western Blot using sera from animals infected with the ...
1
vote
0answers
10 views

Public Pacemaker database online?

I am looking for any database about pacemakers with the leads atria A right ventricle VR left ventricle VL I did not find any in Physionet. Is there any anonymous data about different ...
4
votes
3answers
48 views

Can an influenza virus carry other infectors with it?

This idea came to my mind when thinking about how many people become ill in many locations with similar symptoms (fever and rough cough) from Influenza. There are of course different types of ...
6
votes
3answers
516 views

Should I dilute DNA with water or elution buffer?

I've extracted DNA using a kit and final step is eluted with buffer. If I must dilute my DNA samples, do I use the same elution buffer or can I use milliq water?
1
vote
1answer
46 views

How many possible DNA strands in a single chromatin? [closed]

If a chromosome is replicating but not yet finished is it considered to be a single chromatin with two DNA strands? My question stems from this general biology testing question: Suppose a biologist ...
0
votes
0answers
27 views

Cigarette consumption dose-response function WRT health outcomes

I'm curious how health risks (mortality, lifetime probability of cancer, etc) change with cigarette consumption. Specifically, treatring cigarette consumption like a continuous variable, rather than ...
1
vote
0answers
9 views

How does air-liquid interface (ALI) culture work?

For studying diseases, such as chronic obstructive pulmonary disease (COPD), cells are often grown in air liquid interface. I understand that the common way of establishing this these days is to grow ...
0
votes
0answers
15 views

Terrestrial Vertebrate Limbs

This question is regarding the conversation that took place here: Why don't mammals have more than 4 limbs?. I'm not sure if someone mentioned this and I just missed it, but everyone is talking ...
0
votes
0answers
32 views

Deactivation of plasma cells [closed]

Is it possible to deactivate plasma cells or B-cells? So far I haven't found many answers on the Internet or in Stack Exchange. Please help! Also, please include references. So my ideas were to use ...
1
vote
0answers
43 views

What is the origin of DNA? [closed]

Deoxyribose nucleic acid underpins most life on this earth. It seems to me that the structure of DNA is already reasonably complicated and a certain degree of evolution may have already occured rather ...
3
votes
2answers
109 views

Why do we have to exhale (or inhale) in order to speak?

Every time we speak, sing, or make any other kind of advanced noise with our throats, we exhale, or to put it that way, blow air through our throats. Why is this required? After all, speakers do not ...
1
vote
1answer
24 views

Are more animal species edible than plants?

While grocery shopping I was thinking that we restrict ourselves in regard to which animals we eat. I surely don't want to eat spiders and ants, for example, but I know that this is just because of my ...
1
vote
0answers
18 views

What do you NEED for a 100 meter sprint? [closed]

I was discussing with my friends the 100 meter sprint. Somehow, the topic changed to decreasing the time that the sprint would require: What parts of the human body could you feasibly remove to set a ...
4
votes
1answer
60 views

Why estimate linear and full (linear, quadratic, and correlational) selection coefficients separately?

"We then fitted a linear regression including all three life-history traits to estimate the vector of linear selection gradients, β, for each sex (Lande and Arnold 1983). A quadratic regression ...
0
votes
0answers
11 views

What's the more abundant biopolymer: chitin or (ligno)cellulose?

Chitin is considered the most abundant polymer after cellulose (e.g., Rinaudo, 2006). Does anyone know of specific biomass estimates in literature, probably also differentiating between terrestrial ...
2
votes
2answers
39 views

Hydrophobia Outside of Rabies?

RELATED: Why does rabies cause hydrophobia? Agony, Hydrophobia and viruses in the light of evolutionary principles Has hydrophobia been found outside of rabies? I have only seen it ...
0
votes
1answer
25 views

Deeper Learning in Physiology? [closed]

I am a Pharmacology and Toxicology major at a large state school. I was only required to take physics I and II. (algebra based) At first I thought that was fine, but I soon began to realize that I ...
0
votes
0answers
25 views

Pink discolouration of refrigerated cows milk [on hold]

This morning I discovered a pink partially solid substance floating on my milk (it looked something like the cream on unpasteurised milk but a light pink colour). The milk was a commercial pasteurised ...
1
vote
1answer
29 views

Is this a carpenter ant?

I stumbled upon a few hundred of these swarming around two different places in my living room today. They kind of look like a carpenter ant to me, but I'm not sure. Also, I'd love to know how you ...
2
votes
3answers
33 views

Long term storage of agarose-ethidium bromide gels that have already been cast

I have come up with what I thought was a clever idea: Store the agarose gels I pour, and only cut as many lanes as I need to run later, minimizing wasted agarose (and wasted effort/time) when I need ...
4
votes
0answers
56 views

Long-term effects of antihistamines on mind and CNS [closed]

Can maybe someone share knowledge or guesses on the following questions: Does the prolonged use of antihistamines cause long-term effects on mind or CNS? Are there any known evidences of their ...
1
vote
1answer
45 views

Does physical exercise following lack of sleep increase the risk of sudden cardiac death (SCD)?

Is there any scientific evidence supporting the hypothesis that physical exercise following lack of sleep increases the risk of sudden cardiac death (SCD)? The only relevant research I could find is: ...
1
vote
1answer
20 views

Why are nausea and dizziness such common side effects from medication?

Why are nausea and dizziness such common side effects from medication? If you go through your medicine cabinet and look at side effects, those might just be on every single bottle. Is there some ...

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