3
votes
0answers
120 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
3
votes
0answers
27 views

Why is clownfish attracted to actinia?

I heard that Actinia Equina normally eats what it can catch, but this clownfish here doesn't care much about that: https://www.youtube.com/watch?v=Ughm4mnZ1ls Since the actinia obviously can't hold ...
3
votes
0answers
46 views

Can ingestion of B6 cause gastrointestinal disturbance?

Can the ingestion of vitamin B6 upset the stomach, and if so by what mechanism?
3
votes
0answers
48 views

Excretion of monovalent and divalent ions in sharks

I have heard that sharks excrete $\ce{Na+}$ and $\ce{Cl-}$ by their gill surfaces but divalent ions like magnesium are excreted through feces. What could be the reason behind this?
3
votes
0answers
71 views

Food Intake versus ability to flee among birds, particularly the hummingbird?

Logically speaking, if a hummingbird drinks too much nectar, it will be temporarily overweight and less able or unable to fly to escape danger. However if the same hummingbird doesn't drink enough ...
3
votes
0answers
37 views

Can I purify polyhydroxyalkanoates by heating the cells extensively?

Traditional methods of purifying polyhydroxyalkanoates (PHAs) and other bioplastics made by bacteria involve washing the cells with harsh chemicals or strong bases.I'm interested in maintaining the ...
3
votes
0answers
47 views

What are polytene bands, and why are they there?

Drosophila polytene chromosomes have been particularly useful in genetic research, as it made cytogenetic gene mapping possible with very little effort. This was primarily accomplished due to polytene ...
3
votes
0answers
25 views

A program for cell motility assessment with a batch process function?

Cell motility assessment is a branch of experimental biology or medical science. One example could be an assessment of treatment effects on sperm motility of an animal. The standard procedure involves ...
3
votes
0answers
74 views

How does LCR compare to Assembly PCR

The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
3
votes
0answers
97 views

Is there a standard definition for plant “maturity”?

Is there a species-agnostic metric for identifying plant maturity? There seems to be plenty of literature defining life-history stages for specific crops, but it is not clear if there is a generic ...
3
votes
0answers
50 views

Sequencing rtPCR product

So I have a validated set of primers for rtPCR from Biorad that contains SYBR green. If I do rtPCR, can I use the rtPCR product after purifying it with a Qiagen PCR purification kit? Also, I'm under ...
3
votes
0answers
653 views

What are the factors to consider when determining the sample size for a given study?

Say I'm designing a retrospective study (where I'd collect data from various schools for the last 5 years) to see if consuming milk can contribute to childhood obesity. How do I decide on how many ...
3
votes
0answers
37 views

Are all Autoreceptors Downregulated by their Respective Endogenous Ligands?

I know that the 5-HT1A and $\alpha$2 adrenoreceptors receptors serve as autoreceptors for serotonin (5-HT) and norepinephrine respectively and are down-regulated by repeat exposure to their respective ...
3
votes
0answers
220 views

How does the eugeroic modafinil work?

How does the drug, modafinil (Provigil), exert its eugeroic (wakefulness-promoting) effects? I've read that it works by increasing dopamine and histamine concentrations in the CNS and by serving as a ...
3
votes
0answers
62 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
3
votes
0answers
74 views

What are the physiological effects of wine?

I have read that alcohol in general and wine in particular can have advantageous as well as detrimental effects on human health. Is this true? What are the physiological effects of consuming wine in ...
3
votes
0answers
30 views

What Effect Does the Endocannabinoid System have on Monoaminergic Neurotransmission?

I know that Δ9-tetrahydrocannabinol and other cannabinoids increase dopaminergic neurotransmission in the nucleus accumbens and that this is what results in the euphoria experienced by cannabis users. ...
3
votes
0answers
20 views

Does Doxylamine have any Influence on the Monoaminergic System?

Does doxylamine have any affects on the monoaminergic system? Specifically I am interested in adrenergic or serotonergic activity but additional information on its dopaminergic activity won't hurt ...
3
votes
0answers
91 views

Comparative cost of RNA-seq vs sequencing full length cDNAs

I am in the process of assembling and annotating the genome of a non-model organism, using almost exclusively short read (paired-end Illumina) data. Throughput is one obvious benefit of these data ...
3
votes
0answers
192 views

Why does vision go blurry after microscope use, and how to prevent it?

After using a binocular microscope recently for about 20 minutes, my vision went blurry and for an hour or so it was much harder to read small text. This occurs to a (far) lesser extent whenever I use ...
3
votes
0answers
66 views

Experimental techniques to measure protein-protein and protein-ligand structure and dynamics

We would like to compare our simulation results with experimentation. When one wants to follow experimentally the dynamics (and possibly structure) of protein-protein or protein-ligand docking ...
3
votes
0answers
45 views

Patch-Clamping Neurons Resources

Are there any lectures available on Patch-Clamping? Were can I find a mathematical model of Patch-Clamp? (that can be easily implemented in matlab). I have found some step-by-step protocols on ...
3
votes
0answers
89 views

Terminology question: the scope of an allele in an organism

Let us consider a gene FOO with novel type foo. If I were discussing an organism that has inherited foo in every cell during classical zygote formation, then I would ordinarily just say that the ...
3
votes
0answers
47 views

Literature about putative epigenetic state changes in mammal sequences after cloning steps in Escherichia coli

I would like you to point me out some literature about putative epigenetic state changes in mouse/mammal sequences after cloning steps in Escherichia coli. This are the last search details I used in ...
3
votes
0answers
94 views

Cheapest Way to Measure Germ Density

I would like to measure the surface density of Staphylococcus bacteria and Rhinoviruses (only those two, to be specific) within my home. What's the cheapest way? The textbook procedure is to: rub ...
3
votes
0answers
96 views

RMSD during conformational transition in proteins

When I was investigating the differences between protein structures obtained by X-ray crystallography and NMR spectroscopy, I found the paper [1] compairing structures of several proteins resolved ...
3
votes
0answers
46 views

Physiological or molecular difference before and after sleep?

I know that the details of the process of sleep aren't entirely known, but have always wondered why I feel rested after sleep. Biologically, what differences can be directly observed in the human ...
3
votes
0answers
60 views

What is the frequency of double-hets between parent and child?

Say both parent and child are genotyped for all SNPs. In this setup we are only looking at variant positions between one parent and child - so neither parent nor child are homozygous reference (no ...
3
votes
0answers
91 views

What controls the feeling of discomfort/comfort before and after sleep?

I'm interested in which biological systems or hormones are involved in the following phenomenon: Before sleep it may be difficult to find a comfortable position, and muscular aches and pains are more ...
3
votes
0answers
62 views

Are mature trees as susceptible to mutation from exposure to radiation as seedlings?

If a plant, say a ficus or a teak, as a sapling were exposed to radiation, and a tree of the same species were present at the same distance, which of the two would be more likely to mutate?
3
votes
0answers
44 views

Is there a gene that starts meiosis 2?

Yesterday I thought about a question and asked it to my friend. The question was which gene is completely the same for a male cell that made meiosis 1 recently. My answer was the gene that starts ...
3
votes
0answers
24 views

What metabolically happens when an egg fuses with the nucleus of a somatic cell

In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
3
votes
0answers
125 views

Why are trichomes important for both the roots and shoots of plants?

In what ways are trichomes vital to the plant? Why have them on the plant shoots as well?
3
votes
0answers
34 views

Can a cell culture insert plate be used with a vacuum manifold?

There exists 96 well plate setups for use with vacuum manifolds. Is it possible to use 6/12/24 plate inserts with the same setup?
2
votes
0answers
13 views

How does Halosulfuron-methyl kill nutsedge, while leaving lawn grass and most weeds undamaged?

I use Halosulfuron-methyl to control yellow and purple nutsedge in lawns. This chemical acts by interfering with the acetolactate synthase enzyme, which quickly slows cell division, and growth at all ...
2
votes
0answers
20 views

Hypersensitivity Simultaneously in Ear canals

I got experimental data where hypersensitivity (i.e. tonic pain when touching ear canal) was recognised for about 100 minutes. I do not know which type of hypersensitivity is this one. IgM can ...
2
votes
0answers
16 views

Does a presence of ants on a plant mean that the plant has been infested by aphids?

Ants protect aphids from their predators (such as ladybirds): Aphids and other hemipteran insects secrete a sweet liquid called honeydew, when they feed on plant sap. The sugars in honeydew are a ...
2
votes
0answers
15 views

Why are watermelons sweeter in the center?

Today I had a slice of watermelon and when I reached the outer parts of the pulp I wondered why those are less sweet. Whether or not the sugar occurs in crystalline form in watermelons is still an ...
2
votes
0answers
22 views

Why does skin sometimes suddenly itch when you stretch it?

Sometimes, when I bend over, areas of my skin like my back can suddenly itch quite intensely. Sometimes it may be another part of my body and the pattern seems to be a part of the skin organ that is ...
2
votes
0answers
33 views

Inductance in cell

In an animal cell, especially neuron and in particular its axon, while there is electrical resistance and capacitance mechanism in the cell, which play essential roles in the cable theory model of ...
2
votes
0answers
15 views

what would be the total number of pleura in the exoskeleton of cockroach

basically as far as i know cockroach consists of 10 terga and 9 sterna but what are the total number of pleura present in the cockroach pleura are situated between tergum and sternum of a body ...
2
votes
0answers
39 views

Odds of Fusion of Chromosome #2

There is strong evidence that chromosome 2 in humans is a fusion of two chimp chromosomes (i.e. common ancestor of chimps and humans) as explained at wikipedia here The question is what are the odds ...
2
votes
0answers
27 views

What bird makes this sound?

It sounds like some kind of crow but not really. Here is my recording: https://www.youtube.com/watch?v=4TsKFUugot8&feature=youtu.be#t=06s This bird always wakes me up at 04:00 and this started in ...
2
votes
0answers
18 views

Soft and Hard selection

Seems to me that these two sources (M. Whitlock, B. Wallace) use different definitions of soft and hard selection. M. Whitlock: Soft selection occurs when the relative fitness of an individual is ...
2
votes
0answers
42 views

Can a heterozygous allele show non-heterozygous expression in a family?

I'm doing a family study looking for novel cancer-associated variants in germ-line samples; the goal is to find candidate biomarkers which might be used for early detection. At an earlier step our ...
2
votes
0answers
47 views

Software to calculate Fst from sequence data

I'm looking for a software to calculate Fst from 3 loci DNA data of individuals from a metapopulation. I don't have any prior on the population structure (have no idea of the number of subpopulations ...
2
votes
0answers
34 views

How can cells produce antibodies despite error-checking mechanisms in their genomes?

How can immune cells produce so many different kinds of antibodies with different variable regions if there are so many mechanisms inside the cells that try to keep the DNA sequence as constant as ...
2
votes
0answers
23 views

Questions regarding Scapulohumeral Rhythm

I'm actually an artist, but I wanted to ask this because it will help a lot in my figure drawings. My artistic anatomy teacher taught me about the Scapulohumeral Rhythm. He said that in during the ...
2
votes
0answers
24 views

dbSNP database table desciptions

I am trying to build a local implementation of dbSNP. I would like to know the descriptions of tables listed on ftp://ftp.ncbi.nlm.nih.gov/snp/database/organism_data/human_9606 and on ...
2
votes
0answers
35 views

What is this red liquid produced by a fungal colony?

In a jar, where I had some orchid plants, a fungi start growing few days ago. I removed the plants and let the fungi to expand and grow more. After some days, red drops started to appear on the ...

15 30 50 per page