All Questions

3
votes
0answers
26 views

A program for cell motility assessment with a batch process function?

Cell motility assessment is a branch of experimental biology or medical science. One example could be an assessment of treatment effects on sperm motility of an animal. The standard procedure involves ...
3
votes
0answers
80 views

How does LCR compare to Assembly PCR

The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
3
votes
0answers
112 views

Is there a standard definition for plant “maturity”?

Is there a species-agnostic metric for identifying plant maturity? There seems to be plenty of literature defining life-history stages for specific crops, but it is not clear if there is a generic ...
3
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0answers
787 views

What are the factors to consider when determining the sample size for a given study?

Say I'm designing a retrospective study (where I'd collect data from various schools for the last 5 years) to see if consuming milk can contribute to childhood obesity. How do I decide on how many ...
3
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0answers
37 views

Are all Autoreceptors Downregulated by their Respective Endogenous Ligands?

I know that the 5-HT1A and $\alpha$2 adrenoreceptors receptors serve as autoreceptors for serotonin (5-HT) and norepinephrine respectively and are down-regulated by repeat exposure to their respective ...
3
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0answers
68 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
3
votes
0answers
86 views

What are the physiological effects of wine?

I have read that alcohol in general and wine in particular can have advantageous as well as detrimental effects on human health. Is this true? What are the physiological effects of consuming wine in ...
3
votes
0answers
32 views

What Effect Does the Endocannabinoid System have on Monoaminergic Neurotransmission?

I know that Δ9-tetrahydrocannabinol and other cannabinoids increase dopaminergic neurotransmission in the nucleus accumbens and that this is what results in the euphoria experienced by cannabis users. ...
3
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0answers
23 views

Does Doxylamine have any Influence on the Monoaminergic System?

Does doxylamine have any affects on the monoaminergic system? Specifically I am interested in adrenergic or serotonergic activity but additional information on its dopaminergic activity won't hurt ...
3
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0answers
92 views

Comparative cost of RNA-seq vs sequencing full length cDNAs

I am in the process of assembling and annotating the genome of a non-model organism, using almost exclusively short read (paired-end Illumina) data. Throughput is one obvious benefit of these data ...
3
votes
0answers
71 views

Experimental techniques to measure protein-protein and protein-ligand structure and dynamics

We would like to compare our simulation results with experimentation. When one wants to follow experimentally the dynamics (and possibly structure) of protein-protein or protein-ligand docking ...
3
votes
0answers
48 views

Patch-Clamping Neurons Resources

Are there any lectures available on Patch-Clamping? Were can I find a mathematical model of Patch-Clamp? (that can be easily implemented in matlab). I have found some step-by-step protocols on ...
3
votes
0answers
94 views

Terminology question: the scope of an allele in an organism

Let us consider a gene FOO with novel type foo. If I were discussing an organism that has inherited foo in every cell during classical zygote formation, then I would ordinarily just say that the ...
3
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0answers
106 views

Cheapest Way to Measure Germ Density

I would like to measure the surface density of Staphylococcus bacteria and Rhinoviruses (only those two, to be specific) within my home. What's the cheapest way? The textbook procedure is to: rub ...
3
votes
0answers
109 views

RMSD during conformational transition in proteins

When I was investigating the differences between protein structures obtained by X-ray crystallography and NMR spectroscopy, I found the paper [1] compairing structures of several proteins resolved ...
3
votes
0answers
54 views

Physiological or molecular difference before and after sleep?

I know that the details of the process of sleep aren't entirely known, but have always wondered why I feel rested after sleep. Biologically, what differences can be directly observed in the human ...
3
votes
0answers
112 views

What controls the feeling of discomfort/comfort before and after sleep?

I'm interested in which biological systems or hormones are involved in the following phenomenon: Before sleep it may be difficult to find a comfortable position, and muscular aches and pains are more ...
3
votes
0answers
63 views

Are mature trees as susceptible to mutation from exposure to radiation as seedlings?

If a plant, say a ficus or a teak, as a sapling were exposed to radiation, and a tree of the same species were present at the same distance, which of the two would be more likely to mutate?
3
votes
0answers
25 views

What metabolically happens when an egg fuses with the nucleus of a somatic cell

In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
3
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0answers
142 views

Why are trichomes important for both the roots and shoots of plants?

In what ways are trichomes vital to the plant? Why have them on the plant shoots as well?
3
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0answers
34 views

Can a cell culture insert plate be used with a vacuum manifold?

There exists 96 well plate setups for use with vacuum manifolds. Is it possible to use 6/12/24 plate inserts with the same setup?
2
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0answers
23 views

Any benefit to low dose radiation?

Is there any survival benefit in humans that can be obtained from chronic exposure to low dose radiation, or is any dose of radiation a potential harm? Has any research been done into this?
2
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0answers
20 views

What are parental antagonism & parental conflict?

I've been reading the chapter by Haig et al in The Genetics and Biology of Sexual Conflict. I think this chapter has them quite unclearly defined. Could anyone describe these two phenomenon more ...
2
votes
0answers
31 views

Time for rigor mortis to set in

Rigor mortis sets in due to non-functional "ATP powered calcium pumps" as a result of which the calcium ions stream out & the muscles remain in a suspended animation state. But, it's a frequent ...
2
votes
0answers
23 views

What is the biological basis of “energy” that extraverted humans draw from social engagements and introverts expend?

This question is related to this question about introverts becoming exhausted after extended social events on cognitive sciences stack exchange. As I read more on the topic of human ...
2
votes
0answers
48 views

What proportion of salt in water would make it not dehydrate nor hydrate someone?

Drinking sea water can be deadly as it contains too much salt, basically de-hydrating the body. Normal tap water contains little salt and is good for re-hydration. My question: How much salt needs to ...
2
votes
0answers
27 views

Rosemary room temperature at winter

I consistently read in non-scientific resources that I have to put my rosemary to low temperature (about 10°C) in winter (unless I don't want it to freeze). I googled, but I fail to find a scientific ...
2
votes
0answers
24 views

How can eyeglasses improve vision beyond 100%?

The result of a vision test by my oculist yielded a result of more than 100% while wearing prescription glasses. I wonder, how can a person see better using eyeglasses?
2
votes
0answers
22 views

Which agricultural crops attract rodents?

Are there any data on rodent populations per acre of different types of agricultural crops?
2
votes
0answers
36 views

Pink E.coli cell pellet. Reasons?

I've harvested IPTG induced E.coli BL21DE3 cells' suspension culture by spinning at 5000 rpm/15 min/4 degrees C. The pellet after spin looked pale pink colored. What could be the reasons?
2
votes
0answers
21 views

Converting fmri data to association matrix

I am currently working on a new tool for the analysis of (large) weighted networks. Association matrices (correlation matrices) as obtained from fMRI data[1] are perfect examples of the kind of data I ...
2
votes
0answers
26 views

How to evolutionary explain menstrural cycles synchronization?

Sorry, I can not indicate the source, but I read that menstrual cycles of women who live in the same house over time sync. Is it true? How does it work?
2
votes
0answers
30 views

Imperfect dosage compensation

Dosage compensation is used to make up for different copy numbers of the major sex chromosomes in males and females (One X or Z chromosome in one and two in the other). Two main mechanisms of dosage ...
2
votes
0answers
9 views

Nucleosome wrapping direction

Does anyone know which direction the nucleosomes are wrapped? I wonder: Relative to the B-DNA double helix twist direction (right-hand) Relative to the neighbor nucleosomes. Do they alternate to ...
2
votes
0answers
32 views

How often does bacterial transformation happen?

I have been reading: M. Dröge, A. Pühler, W. Selbitschka, "Horizontal gene transfer among bacteria in terrestrial and aquatic habitats as assessed by microcosm and field studies", Biol. Fertil. ...
2
votes
0answers
40 views

What is the difference between rheobase and threshold?

What is the difference between rheobase and threshold? In the definitions I've read it's described as almost the same thing like it says the threshold is Stimulus whose strength (or voltage) is ...
2
votes
0answers
14 views

Why is gynodioecy common and androdioecy rare?

Gynodioecy is when a population features individuals that produce both male and female gametes and individuals that produce just female gametes. Androdioecy is when a population features individuals ...
2
votes
0answers
18 views

How many cells are there in an apical meristem?

How many cells are there in the apical meristematic tissue? Looking at this picture... I would tend to think that there are few hundreds (maybe only a hundred) cells in the meristem tissue. But I ...
2
votes
0answers
32 views

Ancestral states of sex determination system

Most (maybe all?) species that reproduce sexually have either genders (anisogamy) or mating types (isogamy). There exist today many different type of sexual determination system. There is a whole ...
2
votes
0answers
16 views

What is most ancestral: isogamy or anisogamy?

Sexual reproduction can be feasible with anisogamy (gametes of different sizes i.e. genders) or isogamy (gametes of same size i.e. mating types) or with undifferentiated gametes (i.e. true random ...
2
votes
0answers
45 views

When the mind is highly active, roughly how many neurons become excited in one second?

I'm curios to know how many times neurons send signals in one second when the brain is highly active (Highly active meaning during difficult problem solving, or any task that might bring the brain to ...
2
votes
0answers
23 views

What do we know about the cellular structure, processes, environment, and immediate ancestors of the last universal common ancestor (LUCA)?

I am up for all scientifically sound speculations, and sources are highly welcome. I've looked into this quite a bit myself via scholar.google, the wiki article, and /r/askscience. I'm really looking ...
2
votes
0answers
39 views

Does the Jungian notion of collective unconsciousness have any legitimacy in the light of modern neurobiology and epigenetics?

Carl Jung has long ago proposed a rather controversial notion of collective unconsciousness [1, 2, 3], a form of the unconscious (that part of the mind containing memories and impulses of which ...
2
votes
0answers
34 views

What is the allelic frequency at mutation-selection-drift equilibrium?

mutation-selection equilibrium At mutation-selection equilibrium the expected frequency $f$ of a given locus is $$f ≈ \frac{\mu}{s\cdot h}$$ , where $\mu$ is the mutation rate, $s$ is the selection ...
2
votes
0answers
64 views

How many proteins are synthesized in human body throughout life?

My idea to calculate this is: Define up to what age will do the calculation. (for instance 60) Determine how many cells born and died up to that point in life. (by type of cell) Determine how many ...
2
votes
0answers
92 views

Questions about insects eating flour and living in the cracks of a wooden cutting boards

I have a large wooden cutting board which is used in kitchen mainly for preparing egg pasta. The wood type is Populus (poplar or aspen or cottonwood). The main ingredients used with the cutting board ...
2
votes
0answers
26 views

Can Galactose be synthesized with photosynthesis?

I know that galactose can be found in sugar beets and some gums so I thought plants could synthesize galactose but in my book the answer to the question "Which of these can be synthesized with ...
2
votes
0answers
28 views

Biology Experiment Data (Hodgkin-Huxley)

I'm doing research into the Hodgkin-Huxley Model from an electronics/mathematics perspective and I'm looking to find actual numerical results from experiments on squid axons. I want to compare the ...
2
votes
0answers
18 views

Some sort of Lymantriidae caterpillar?

I found this larva in Sweden in July. It was about 15 mm long. It reminded me of Orgyia antiqua that I've seen before, but smaller and with a somewhat different colouration.
2
votes
0answers
23 views

Where can I upload non-human Genotype data?

I have genotype data from few chicken population and I want to (need to) upload them somewhere online with free access. I have searched the web but I haven't found any place for non-human genotype ...

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