3
votes
0answers
57 views

Is it possible to isolate cells harboring a specific DNA sequence?

Suppose on cell subpopulation harbours within its genome a specific DNA sequence that do not exist in other cell subpopulations. Is it technically possible to isolate such subpopulation ? and if yes, ...
3
votes
0answers
25 views

Fur genetics - what are the causes of fur colour decision?

This Australian Shepherd puppy's father is a black-tri; mother is a blue merle. What would cause this dog to have so much white color? Are there any general explanations as to why this might ...
3
votes
0answers
32 views

Why are kidney discard rates so high?

A recent report from UNOS states: The kidney discard rate has returned to pre-KAS levels, dropping from 20.2 percent in the first six months to 18.4 percent in months 7-10. To me, this seems ...
3
votes
0answers
33 views

Games with non-uniform interaction rates

Background: Many models in evolutionary game theory assume uniform interaction rates. For instance, consider the $2\times 2$ game: \begin{array}{l c c} & A & B \\ A & a & b \\ ...
3
votes
0answers
60 views

How can I find the mRNA sequence for a specific prokaryotic gene?

What I want to find out is the start of the transcription for a specific gene, how long the UTR is before the actual coding sequence starts. I've looked at various databases like NCBI Gene, Refseq or ...
3
votes
0answers
21 views

What are the metabolic consequences of negative mechanical work in muscle contractions?

When a muscle contracts while it shortens (concentric), it consumes ATP I believe primarily due to cross-bridge shortening to remove the myosin head from it's attached state. However, when a muscle ...
3
votes
0answers
21 views

Access and decay time of long-term memory

As for STM (short-term memory), access time is 70 ms and decay time is 200 ms. Is there any calculated time for LTM (long-term memory) also? And does LTM really decay with the passage of time?
3
votes
0answers
24 views

How do I test if microbes have survived after dehydration?

I have a solution containing various bacteria and fungi. My aim is to place solution on filter paper, and wait until it dries. I then wish to test if the organisms have survived, either on the dried ...
3
votes
0answers
32 views

Relative densitometry from SDS PAGE

I'd like to perform densitometry on a Coomassie stained SDS PAGE gel to compare a recombinant protein's expression levels under two conditions. I'm using BioRad's Image Lab software. My questions are ...
3
votes
0answers
32 views

Can body temperature affect the quality of sleep?

If so, how does it influence our sleep? And what can we do to improve the quality of sleep by adjesting our body tempurature?
3
votes
0answers
25 views

Why do teeth ache after fillings?

I had a filling which involved drilling the tooth, but the drilling did not touch the nerve. According to the x-ray taken at the same time, the nerve is in fact some distance from where the filling ...
3
votes
0answers
41 views

How long a dog can remember its owner's smell?

Can dog remember multiple smells? and how long it can remember a smell?
3
votes
0answers
48 views

What is a common body composition in mammals?

The Physiology Of The Racing Greyhound states that most species have about a 40% muscle mass to body mass ratio. Understanding Body Composition puts "optimal fitness" in humans at 16-25% body fat for ...
3
votes
0answers
47 views

What are the sizes of the cells that make up human hair?

The question is in the title, but I'll explain why the question arose. I'm curious about the rates that various cells in the body divide, and have found various information relating to this, but ...
3
votes
0answers
66 views

What is the essence of difference of how different chemicals affect the same receptor?

It is known that various chemicals can bind to the same receptor type, producing different effects. Be these chemicals agonists or antagonists, there are more variations in how they influence the ...
3
votes
0answers
43 views

Caterpillar identification

It has since cocooned I think but it was a weird circumstance. I kept adding leaves for it to eat, and it decided it was done, and cocooned under a wilted leaf. I'm wondering if anyone can ID the ...
3
votes
0answers
59 views

Did I just watch a spider die?

So I'm sitting in my office and all of a sudden a small spider is gliding down on a web string from the ceiling, right in front of my eyes, and down onto my desk. It proceeds to make a few awkward ...
3
votes
0answers
21 views

What techniques can be used to link environmental conditions to crop yield?

Im interested in how we can determine what are the optimal conditions for crops and how deviations from these optimal conditions affect yield. In the literature I have found two main approaches and I ...
3
votes
0answers
35 views

Do ginko trees grow well in Eastern North America (specifically Ontario,Canada)

I have recently noticed many Ginko (Ginko biloba) saplings planted in my Eastern Ontario city (in Canada), and they are looking a bit sickly, with yellow leaves. They seem to be a popular choice in ...
3
votes
0answers
38 views

dominance coefficient, why (1+k)?

I'm trying to get a better understanding of quantitative genetics by reading Lynch and Hill 1986. I'm getting stuck with some notation that's used right at the beginning referring to the mutant and WT ...
3
votes
0answers
73 views

Cause-and-effect questions about growth and development of plant

I need to solve some cause-and-effect problems. The problems are related to growth and development of plant. "Growth and Development" chapter is the first chapter in third level of high school (senior ...
3
votes
0answers
86 views

L-tryptophan in mammalian cell culture

Why must the concentration of L-tryptophan be kept to a relatively low level when culturing mammalian cells? It's an essential amino acid, so I cannot fathom why low concentrations as opposed to ...
3
votes
0answers
23 views

How is atrophied muscle different than normal muscle in a chemical or biological way?

I am a writer with a question for a story I'm working on. In it, the character suffers from muscle atrophy due to spinal damage. So that I can portray this accurately, what would cause this type of ...
3
votes
0answers
72 views

What is this ground dwelling, funnel web spider in Montana?

I've killed dozens of what appears to be the same type of spider. I've included pictures of 2 individuals. I've seen them in all sizes from 1-4". I'm in Missoula Montana. They're aggressive, they ...
3
votes
0answers
50 views

What Chemical Trigger Causes Ectomycorrhiza To Change From Asexual To Sexual?

I am searching for the catalyst behind the change of asexual to sexual ectomycorrhiza when symbiosis with a tree root is formed. As ectomycorrhiza attaches itself to a root, they form a relationship ...
3
votes
0answers
24 views

Photosynthetic efficiency and light harvesting efficiency

Is there some sort of measure of the light harvesting efficiency (possibly like transfer time) that is directly related to the photosynthetic efficiency in some sort of equation? Also, how important ...
3
votes
0answers
217 views

Can HIV mutate to become airborne?

HIV has a very high mutation rate due to its inability to correct errors in DNA replication and it is very adaptable to its environment. Hence I wonder whether is it possible for it to mutate such ...
3
votes
0answers
81 views

How long does conception take?

I know that conception is a multi-step process. A sperm fuses with an egg. Its contents diffuse into the cytoplasm. Dad's DNA is transported to the nucleus. Proteins are gradually made from Dad's ...
3
votes
0answers
35 views

Looking for a dichotomous key to identify primates

I'm looking for a dichotomous key to identify primates by physical and possibly geographical traits. Any format is fine (print, web, mobile app, etc.). Other than classroom activities and genetic ...
3
votes
0answers
40 views

How large might a paper wasp nest become?

About two weeks ago I noticed a new wasp nest in my back yard. I generally leave them as long as they aren't interfering with anything, but this nest has suddenly grown quite large and they are ...
3
votes
0answers
33 views

How do paper wasp larvae not fall from their nest?

There is now a paper wasp nest hanging from the eaves of my bedroom. Not very big, I supposed about a hundred chambers and about 7 to 10 adults in total. I observe it every now and then. It just ...
3
votes
0answers
44 views

Do High Triglycerides Invalidate Tests for Infections?

Blood donation websites tell you to avoid eating fats a few days before the donation, because when there is a lot of fat in the blood, they can't test for infectious diseases and must discard the ...
3
votes
0answers
38 views

Negative association between habitat quality and reproductive success in birds

I'm looking for bird studies that have found either a negative correlation or no correlation between habitat quality and reproductive success. Or in other words, bird studies that have found that ...
3
votes
0answers
144 views

What is the name of this bird?

Does anyone know the name of the bird in the pictures? It was found in the south of Spain in the month of June. (it is certainly neither a house sparrow nor a swallow nor a robin). Thanks for the ...
3
votes
0answers
70 views

How to understand relatedness in an infinite island model?

My understanding is that the relatedness coefficient in kin selection models measures positive assortment. That is, altruism is more likely to evolve if altruists tend to interact with other ...
3
votes
0answers
76 views

How stable is linear DNA transfected in eukaryotic cells?

I would like to know, with references from the literature, what is the half life of a linear dsDNA transfected in a mammalian cells. For example, if I transfect human cells with a PCR product ...
3
votes
0answers
38 views

Expected reproduction rate of a dandelion and/or fern

I am working a basic mathematical/computer simulation of an ecosystem, and I need to know the birth rate of each organism in my simulation. However, I could not find any data for how often a small, ...
3
votes
0answers
18 views

Gene silencing: relative or absolute number of methylated CpG sites?

CpG content varies a lot across the human genome. What does determine a promoter to be silenced through DNA methylation: the fraction of CpG sites that are methylated or the total number of methylated ...
3
votes
0answers
104 views

Can I leave BL21(DE3) cells in room temperature?

I am preparing competent cells, and I finished inoculating a single colony in SOB. It has been incubating at 37 degrees Celsius for almost 16 hours since, and it's getting very late where I live. I ...
3
votes
0answers
94 views

Mating types in fungi (and somatogamy)

I would have some related questions about the mating types of fungi. Does a single spore generate a mycelium possessing only one mating type? If it does not in general, do Ascomycota and ...
3
votes
0answers
55 views

What is effectiveness of methyl-alcohol as sporicide?

Methyl-alcohol is the weakest alcohol in terms of bactericidal properties. However, I have read that it also works as sporicide. What is the effectiveness of methyl-alcohol in killing the spores of ...
3
votes
0answers
51 views

When and How do pacemaker cells develop during the cell aggregation process of Dictyostelium discoideum?

I was reading a paper by Tang & Othmer about oscillations and waves in Dictyostelium discoideum. Under certain condition like starvation period in the life cycle of a Dictyostelium discoideum ...
3
votes
0answers
99 views

Pits and Pores in Vascular Tissue

What purpose is served by the pits and pores in xylem and phloem cells? I cant find what purpose that these components serve in the vascular tissues in plants
3
votes
0answers
243 views

E. coli not growing in liquid medium

We are regularly doing bacterial transformation and subculture from plate to liquid media to extract DNA. This usually goes very well and is straightforward, but occasionally, the colonies that grew ...
3
votes
0answers
270 views

Storing Taqman plates with polymerase in fridge before running

I'm going to be running some Taqman assays on cDNA generated from RNA collected from various samples, and will end up running a bunch of plates (right now the setup is 1/2 a plate per sample, ...
3
votes
0answers
70 views

Neural coupling between Pinsky-Rinzel (reduced Traub) neuron with a Wang-Buzsaki neuron?

I have read two papers related to modeling neurons. The first one is the paper by Pinsky and Rinzel and the second one by Wang and Buzsaki. The first mentioned neuron model is a excitatory neuron ...
3
votes
0answers
66 views

Is the number of stamens always 9 for geranium?

We are studing in class the reproduction of plants. We start the lesson by observing some components of flowers, it was the Géranium. First of all we start counting the number of sepals , petals and ...
3
votes
0answers
37 views

Multiplex PCR, shorter amplicon inhibiting longer amplicon?

I want to run multiplex PCRs for my genotyping, with a primer pair targeting my construct and a primer pair targeting some housekeeping gene (sort of a built-in control). I designed the control ...
3
votes
0answers
29 views

Generate T2/T1 Phage Resistant E Coli

I would like to generate T2/T1 phage resistant Stbl3 E Coli to use in virus production. Is there a plasmid somebody has used that confers resistance, or is this done another way?
3
votes
0answers
103 views

From where is the pollen kit material secreted?

From where is the pollen kit material secreted? I think it the tapetum would be a good candidate, since it is provides nutrition to the pollen grains.

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