3
votes
0answers
42 views

Are all Autoreceptors Downregulated by their Respective Endogenous Ligands?

I know that the 5-HT1A and $\alpha$2 adrenoreceptors receptors serve as autoreceptors for serotonin (5-HT) and norepinephrine respectively and are down-regulated by repeat exposure to their respective ...
3
votes
0answers
72 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
3
votes
0answers
94 views

What are the physiological effects of wine?

I have read that alcohol in general and wine in particular can have advantageous as well as detrimental effects on human health. Is this true? What are the physiological effects of consuming wine in ...
3
votes
0answers
34 views

What Effect Does the Endocannabinoid System have on Monoaminergic Neurotransmission?

I know that Δ9-tetrahydrocannabinol and other cannabinoids increase dopaminergic neurotransmission in the nucleus accumbens and that this is what results in the euphoria experienced by cannabis users. ...
3
votes
0answers
23 views

Does Doxylamine have any Influence on the Monoaminergic System?

Does doxylamine have any affects on the monoaminergic system? Specifically I am interested in adrenergic or serotonergic activity but additional information on its dopaminergic activity won't hurt ...
3
votes
0answers
79 views

Experimental techniques to measure protein-protein and protein-ligand structure and dynamics

We would like to compare our simulation results with experimentation. When one wants to follow experimentally the dynamics (and possibly structure) of protein-protein or protein-ligand docking ...
3
votes
0answers
115 views

Cheapest Way to Measure Germ Density

I would like to measure the surface density of Staphylococcus bacteria and Rhinoviruses (only those two, to be specific) within my home. What's the cheapest way? The textbook procedure is to: rub ...
3
votes
0answers
118 views

RMSD during conformational transition in proteins

When I was investigating the differences between protein structures obtained by X-ray crystallography and NMR spectroscopy, I found the paper [1] compairing structures of several proteins resolved ...
3
votes
0answers
57 views

Physiological or molecular difference before and after sleep?

I know that the details of the process of sleep aren't entirely known, but have always wondered why I feel rested after sleep. Biologically, what differences can be directly observed in the human ...
3
votes
0answers
123 views

What controls the feeling of discomfort/comfort before and after sleep?

I'm interested in which biological systems or hormones are involved in the following phenomenon: Before sleep it may be difficult to find a comfortable position, and muscular aches and pains are more ...
3
votes
0answers
68 views

Are mature trees as susceptible to mutation from exposure to radiation as seedlings?

If a plant, say a ficus or a teak, as a sapling were exposed to radiation, and a tree of the same species were present at the same distance, which of the two would be more likely to mutate?
3
votes
0answers
158 views

Why are trichomes important for both the roots and shoots of plants?

In what ways are trichomes vital to the plant? Why have them on the plant shoots as well?
2
votes
0answers
18 views

How long can Cholera bacteria survive in a dead host?

How long can cholera bacterium survive inside a dead host? Can they remain dormant in such conditions? BACKGROUND On a hill not far from where I live, there was a hospital operating since 16th until ...
2
votes
0answers
27 views

Sensitivity of eyes to flashing lights

My eyes are very sensitive to flashing lights - for example I'm always the first person to notice that a fluorescent tube is about to fail because I see it flickering when other people can't. When ...
2
votes
0answers
21 views

Degree of dosage compensation in Drosophila melanogaster

In this paper the authors state that the dosage compensation seen in Drosophila is approximately twofold, but they do not provide any source or numbers (as far as I can see) for this. What is the mean ...
2
votes
0answers
18 views

Protein-Bound Drugs in Kwashiorkor

Kwashiorkor is characterised by decreased serum protein (including serum albumin) due to poor dietary protein intake. What is the effect of this lowered serum protein on highly-protein-bound drugs? ...
2
votes
0answers
35 views

What's the (or some of the) minimum(s) amount of atmospheric carbon dioxide needed by plants?

We currently have a problem of increasing CO2 in the atmosphere. But assuming we find a way to carbon sink it, what is the minimum CO2 we need to leave in the atmosphere to provide a source for ...
2
votes
0answers
16 views

protein with highest affinity to lactose

I was wondering: What is the protein with the highest affinity to lactose ? Are there some databases with values for affinity ? Thanks in advance
2
votes
0answers
33 views

Parasites with both plant and animal hosts?

Are there animals (or plants) that are parasitic on both animals and plants? Especially such that they rely on their plant host being eaten by their animal host?
2
votes
0answers
25 views

What makes substances allergenic?

Is it possible to tell or rule out (potentially a priori) whether any given substance or compound is likely and how likely to cause allergies with high confidence, without the need to conduct ...
2
votes
0answers
17 views

Immediate stroke/seizure Therapy

Can an oncoming stroke/seizure, be stopped by a setup consisting of a brain-computer-interface (BCI) that immediately detects the variation in wave-form, and then another machine that neutralizes the ...
2
votes
0answers
22 views

Normal ECG/EKG Measurement?

I'm getting started using ECG using a 3 lead system by iWorx. I have place the leads on my two wrists and the ground on my ankle and have recorded some data into the provided LabScribe 3 software ...
2
votes
0answers
48 views

Help needed in fish identification

Please help me identify these fish. I looked around but wasn't able to determine its species.
2
votes
0answers
32 views

Human leukocytes (re)circulation/migration in homeostatic state

One can easily find information on the topic of leukocytes trafficking between vessels and peripheral tissues during inflammation. But what happens during normal states when there is no pathology? ...
2
votes
0answers
38 views

Why did the Brain develop in the front in most organisms?

I was wondering my most, well pretty much all organism with a brain have it right in front of their bodies or at the top.
2
votes
0answers
74 views

What's Incomplete atrioventricular heart block (AV)?

This is unclear for me and I can not understand differences between complete and incomplete atrioventricular heart block.also I want an electrocardiograph of incomplete atrioventricular heart block.
2
votes
0answers
25 views

Neural correlates of the function of the cardiovascular system?

Are there any known neural correlates of the function of the cardiovascular system? I'm looking for research results that use standard brain imaging techniques (e.g. electroencephalography, ...
2
votes
0answers
20 views

Protein Design - Target Structure Specification

I'm curious about how protein structures are defined in general, but in particular, I'm wondering how a target structure can be specified without knowing the amino acid sequence. For example, in ...
2
votes
0answers
18 views

background extraction in microscope analysis

I have taken microscope pictures for proteins in HeLa cells, in order to quantify the intensity (immunofluorescence). The proteins are both at the nuclear envelope, and in order to quantify the ...
2
votes
0answers
26 views

Expression/Mechanism of ROR1 in healthy tissue

ROR1 is currently under investigation as a therapeutic target for cancer (1). A number of studies show different cancers may have their metastatic potential reduced, or become apoptotic through ...
2
votes
0answers
29 views

Is there a standard formula/calculation for sexual dimorphism?

Is there a standard, or most accepted, way to present a value for sexual dimorphism? For example, one could use $$\frac{\text{Female value}}{\text{Male value}} -1$$ This would give negative values ...
2
votes
0answers
33 views

How to get a pdb file of a single atom?

I am trying to get a pdb file that contains a single atom, it doesn't matter if it's Hydrogen or another atom. How can I obtain this? Are there any pdb files with just a single atom available to ...
2
votes
0answers
43 views

Up to date, extensive documentary about dinosaurs?

I just finished reading the Wikipedia page about dinosaurs, and I very much enjoyed it. I knew that much of what I learned in the 80's as a child is (and was) incorrect, and now that I am a parent, I ...
2
votes
0answers
20 views

What is the most complex biological organism (or precursors) that we have been able to synthesize from raw materials?

In the Miller–Urey experiment they produced several amino acids. I'm not sure if there were other similar experiments that got further. ...
2
votes
0answers
38 views

eradicating h.pylori naturally

This question is not to discuss whether substances with anti-h.pylori agents works or not, or the pros and cons of such but from a medical perspective. Assuming that one manage to control, reduce, or ...
2
votes
0answers
37 views

How long can a human neuron cell live outside the body in a controlled environment?

Have there any experiments been to keep neurons alive (stationary) without preserving methods such as freezing? If yes, for how long?
2
votes
0answers
31 views

Does the metabolic rate determine how fast the telomeres shorten?

In many papers one can read that telomeres may play an important role in longevity. According to Calado et al.1 the telomeres of mice are much longer than the telomeres of humans. However, mice have ...
2
votes
0answers
17 views

Cockroaches and magnetic fields

Aloha, I live in Hawaii working with sharks and their sensitivity to magnetism but here we also have large cockroaches. Has anyone worked with cockroaches and magnetism? I wonder if placing high ...
2
votes
0answers
30 views

How Many Generation We can Run a Mycelium of Pleurotus ostreatus/Pearl Oyster?

I am able to grow mycelium successfully on the spent coffee grounds. And mycelium is successfully fruiting once/twice on the same substrate. I am cloning by taking mycelium from the existing one ...
2
votes
0answers
20 views

Why are riboswitches mostly present in bacteria and not in eukaryotes?

Riboswitches are a rather elegant way to regulate gene expression without any additional machinery. A small ligand binds to the mRNA and directly influences transcription or translation. Most of the ...
2
votes
0answers
38 views

Can protein sample be made 2% Triton X-100 free?

The protein I am purifying needed an elution buffer with 2% Triton X-100. I formulated the elution buffer not keeping the CMC in mind. My goal is to make my protein sample triton free to check its ...
2
votes
0answers
21 views

What has to duplicate before cell enters M phase?

What has to duplicate before cell enters M phase? DNA histones centrosome mitochondria (any other organelles?) condensins (not sure?) have I forgotten anything? Thank you in advance!
2
votes
0answers
20 views

What are the biological mechanisms of candy causing nausea?

As I sit here after eating too much chocolate, I wonder: What are the biological mechanisms behind eating too much candy candy causing nausea in a healthy individual? Is it a spike in blood sugar, ...
2
votes
0answers
47 views

How to prevent e coli from clumping (for FACS)?

I'm performing FACS on e coli, but the cells are clumping together so each event is multiple cells. I ran a control where I had one flask of e coli expressing GFP, and one flask expressing RFP. Run ...
2
votes
0answers
27 views

Why does human hair grow and behave differently depending on its location?

Why does human hair behave differently depending on its location? For instance, head hair grows to an indefinite length, but eyebrows maintain a particular length and appearance. Head hair and ...
2
votes
0answers
28 views

Double stranded is more 'durable' than single stranded stuff?

I'm struggling with a question I've been asked. "Why is double stranded genetic material more 'durable' than single stranded one?" I know that double stranded genetic material is more stable due to ...
2
votes
0answers
44 views

Why do we find some things disgusting and others delicious?

For example, why do I love paprika while my girlfriend hates it? Why do I find broccoli disgusting and she adores it? As humans, there are things we'd all agree on being awful, like rotten meat, for ...
2
votes
0answers
30 views

How does the population fitness changes after a change in mutation rate

The mean population fitness as given by mutation load theory depends only on the genome-wide mutation rate ($U$). My question is: how many generations is needed to reach a new mutation load ...
2
votes
0answers
20 views

Color perception vs shade perception demo

I can't seem to find one of the best demos I've seen of color vs brightness perception. It consisted of a rotating animation of earth made of red of bright red points on a dark green background. As ...
2
votes
0answers
27 views

How do I get recombinant proteins into the nucleus of mammal cells?

I know that there are Nuclear Localisation Sequencenes (NLS). They can be taken from endogenous or viral proteins and fused to the N or C terminus of my recombinant protein. Which is the best one? ...

15 30 50 per page