2
votes
0answers
17 views

How long can a human neuron cell live outside the body in a controlled environment?

Have there any experiments been to keep neurons alive (stationary) without preserving methods such as freezing? If yes, for how long?
2
votes
0answers
16 views

Cockroaches and magnetic fields

Aloha, I live in Hawaii working with sharks and their sensitivity to magnetism but here we also have large cockroaches. Has anyone worked with cockroaches and magnetism? I wonder if placing high ...
2
votes
0answers
40 views

How long does it take to form a peptide bond?

What is the time taken to form a peptide bond in vivo or in vitro? It isn't mentioned in my course on protein structures. I was just curious to find out if any time scale is known? Given that ...
2
votes
0answers
6 views

How Many Generation We can Run a Mycelium of Pleurotus ostreatus/Pearl Oyster?

I am able to grow mycelium successfully on the spent coffee grounds. And mycelium is successfully fruiting once/twice on the same substrate. I am cloning by taking mycelium from the existing one ...
2
votes
0answers
17 views

Why are riboswitches mostly present in bacteria and not in eukaryotes?

Riboswitches are a rather elegant way to regulate gene expression without any additional machinery. A small ligand binds to the mRNA and directly influences transcription or translation. Most of the ...
2
votes
0answers
29 views

Can protein sample be made 2% Triton X-100 free?

The protein I am purifying needed an elution buffer with 2% Triton X-100. I formulated the elution buffer not keeping the CMC in mind. My goal is to make my protein sample triton free to check its ...
2
votes
0answers
20 views

What has to duplicate before cell enters M phase?

What has to duplicate before cell enters M phase? DNA histones centrosome mitochondria (any other organelles?) condensins (not sure?) have I forgotten anything? Thank you in advance!
2
votes
0answers
17 views

What are the biological mechanisms of candy causing nausea?

As I sit here after eating too much chocolate, I wonder: What are the biological mechanisms behind eating too much candy candy causing nausea in a healthy individual? Is it a spike in blood sugar, ...
2
votes
0answers
19 views

How to prevent e coli from clumping (for FACS)?

I'm performing FACS on e coli, but the cells are clumping together so each event is multiple cells. I ran a control where I had one flask of e coli expressing GFP, and one flask expressing RFP. Run ...
2
votes
0answers
28 views

Is it possible that a set of functionally related proteins in a pathway fulfill different functions?

Could it be that a given pathway of enzymes (or proteins in general) may fulfill different purposes in a cell by for shifting partners? Say protein A activates B, B activates C and C has a specific ...
2
votes
0answers
24 views

Double stranded is more 'durable' than single stranded stuff?

I'm struggling with a question I've been asked. "Why is double stranded genetic material more 'durable' than single stranded one?" I know that double stranded genetic material is more stable due to ...
2
votes
0answers
37 views

Why do we find some things disgusting and others delicious?

For example, why do I love paprika while my girlfriend hates it? Why do I find broccoli disgusting and she adores it? As humans, there are things we'd all agree on being awful, like rotten meat, for ...
2
votes
0answers
21 views

How does the population fitness changes after a change in mutation rate

The mean population fitness as given by mutation load theory depends only on the genome-wide mutation rate ($U$). My question is: how many generations is needed to reach a new mutation load ...
2
votes
0answers
17 views

Color perception vs shade perception demo

I can't seem to find one of the best demos I've seen of color vs brightness perception. It consisted of a rotating animation of earth made of red of bright red points on a dark green background. As ...
2
votes
0answers
24 views

How do I get recombinant proteins into the nucleus of mammal cells?

I know that there are Nuclear Localisation Sequencenes (NLS). They can be taken from endogenous or viral proteins and fused to the N or C terminus of my recombinant protein. Which is the best one? ...
2
votes
0answers
13 views

Resource for finding Repressive/Inhibitory factors for a given gene?

I have a list of genes for each of which I'd like to find: A list of transcription factors that up-regulate the gene A list of inhibitory factors that down-regulate it. I used this tool on ...
2
votes
0answers
42 views

What are the common methods to estimate additive genetic variance?

Additive genetic variance can be estimated in a number of ways, and is a key concept in evolutionary biology and quantitative genetics. What are the typical methods (experimental designs) used to ...
2
votes
0answers
33 views

What is the biological basis of “energy” that extraverted humans draw from social engagements and introverts expend?

This question is related to this question about introverts becoming exhausted after extended social events on cognitive sciences stack exchange. As I read more on the topic of human ...
2
votes
0answers
54 views

What proportion of salt in water would make it not dehydrate nor hydrate someone?

Drinking sea water can be deadly as it contains too much salt, basically de-hydrating the body. Normal tap water contains little salt and is good for re-hydration. My question: How much salt needs to ...
2
votes
0answers
33 views

Rosemary room temperature at winter

I consistently read in non-scientific resources that I have to put my rosemary to low temperature (about 10°C) in winter (unless I don't want it to freeze). I googled, but I fail to find a scientific ...
2
votes
0answers
27 views

Which agricultural crops attract rodents?

Are there any data on rodent populations per acre of different types of agricultural crops?
2
votes
0answers
52 views

Pink E.coli cell pellet. Reasons?

I've harvested IPTG induced E.coli BL21DE3 cells' suspension culture by spinning at 5000 rpm/15 min/4 degrees C. The pellet after spin looked pale pink colored. What could be the reasons?
2
votes
0answers
39 views

Imperfect dosage compensation

Dosage compensation is used to make up for different copy numbers of the major sex chromosomes in males and females (One X or Z chromosome in one and two in the other). Two main mechanisms of dosage ...
2
votes
0answers
13 views

Nucleosome wrapping direction

Does anyone know which direction the nucleosomes are wrapped? I wonder: Relative to the B-DNA double helix twist direction (right-hand) Relative to the neighbor nucleosomes. Do they alternate to ...
2
votes
0answers
21 views

Why is gynodioecy common and androdioecy rare?

Gynodioecy is when a population features individuals that produce both male and female gametes and individuals that produce just female gametes. Androdioecy is when a population features individuals ...
2
votes
0answers
38 views

Ancestral states of sex determination system

Most (maybe all?) species that reproduce sexually have either genders (anisogamy) or mating types (isogamy). There exist today many different type of sexual determination system. There is a whole ...
2
votes
0answers
62 views

When the mind is highly active, roughly how many neurons become excited in one second?

I'm curios to know how many times neurons send signals in one second when the brain is highly active (Highly active meaning during difficult problem solving, or any task that might bring the brain to ...
2
votes
0answers
53 views

Does the Jungian notion of collective unconsciousness have any legitimacy in the light of modern neurobiology and epigenetics?

Carl Jung has long ago proposed a rather controversial notion of collective unconsciousness [1, 2, 3], a form of the unconscious (that part of the mind containing memories and impulses of which ...
2
votes
0answers
71 views

Mutation-Selection-Drift Equilibrium

mutation-selection-drift equilibrium is one of the most important concept of population genetics. I could easily find the calculations for mutation-secltion equilibrium and for mutation-drift ...
2
votes
0answers
81 views

How many proteins are synthesized in human body throughout life?

My idea to calculate this is: Define up to what age will do the calculation. (for instance 60) Determine how many cells born and died up to that point in life. (by type of cell) Determine how many ...
2
votes
0answers
114 views

Questions about insects eating flour and living in the cracks of a wooden cutting boards

I have a large wooden cutting board which is used in kitchen mainly for preparing egg pasta. The wood type is Populus (poplar or aspen or cottonwood). The main ingredients used with the cutting board ...
2
votes
0answers
28 views

Can Galactose be synthesized with photosynthesis?

I know that galactose can be found in sugar beets and some gums so I thought plants could synthesize galactose but in my book the answer to the question "Which of these can be synthesized with ...
2
votes
0answers
39 views

Biology Experiment Data (Hodgkin-Huxley)

I'm doing research into the Hodgkin-Huxley Model from an electronics/mathematics perspective and I'm looking to find actual numerical results from experiments on squid axons. I want to compare the ...
2
votes
0answers
22 views

Some sort of Lymantriidae caterpillar?

I found this larva in Sweden in July. It was about 15 mm long. It reminded me of Orgyia antiqua that I've seen before, but smaller and with a somewhat different colouration.
2
votes
0answers
66 views

How likely is it that through dog breeding, tail-wagging has been disassociated with happiness?

I am not a biologist, so sorry to anybody for misinformation in this post. If I understand correctly, dogs evolved the ability to show emotions through various sounds and tail-wagging in order to ...
2
votes
0answers
38 views

Where can I upload non-human Genotype data?

I have genotype data from few chicken population and I want to (need to) upload them somewhere online with free access. I have searched the web but I haven't found any place for non-human genotype ...
2
votes
0answers
35 views

TV's so loud when I turn over

Scenario: I'm about to fall asleep with the T.V. on. Having completely forgotten that it was, no longer even hearing it, just before falling asleep I turn over with a 'fresh' ear and it's blaring. If ...
2
votes
0answers
21 views

Mutation-drift equilibrium and among loci variance in heterozygosity

At mutation-drift balance, the increased heterozygosity brought by new mutations is exactly equal to the loss of heterozygosity due to genetic drift. At equilibrium, the expected heterozygosity for a ...
2
votes
0answers
19 views

Can two Hfr strains of E. coli conjugate?

Genetics textbooks (and some internet searching) yield abundant examples of Hfr strains conjugating with F- cells, but these sources are surprisingly silent regarding the results of an Hfr ...
2
votes
0answers
16 views

Metabolism that generates methanol

I've been looking for help topics about methanol in metabolism. Specifically I wish to know What is a common dietary component that generates methanol following metabolism and comment on its ...
2
votes
0answers
25 views

What is Colloidal biology and does it have any scientific background?

There is the following bulletin published for the History of Chemistry Vol. 32: 105-118 in 2007: “MOLECULAR” VERSUS “COLLOIDAL”: CONTROVERSIES IN BIOLOGY AND BIOCHEMISTRY, 1900–1940* written by PhD ...
2
votes
0answers
35 views

Why Hasn't my Cactus Grown Deadly Spines?

I recently purchased a small cactus plant from my local store for about $5. I've been giving the cactus lots of sunlight and a good watering every week. I've noticed that since I bought it, the ...
2
votes
0answers
61 views

Origin of the 260/280 ratio?

This is not a duplicate of all the other 260/280 ratio questions, I already know that DNA is supposed to be 1.8 and RNA is supposed to be 2.0. However, this might be more appropriate for chemistry, ...
2
votes
0answers
32 views

Fatty Acid Synthesis of Fats Longs than 16 C

I understand that the human body when performing Fatty Acid Synthesis can synthesize only until C16 (palmitate). However the ER has desaturases and elongases. I know that desaturases are used to add ...
2
votes
0answers
29 views

Genome-wide methylation analysis: sources of technical error?

We are doing a genome-wide analysis of methylation via bisulfite sequencing for an insect species. Previous experimental techniques have suggested the presence of methylation in this organism, ...
2
votes
0answers
22 views

What should be the distance between plant promoter and gene?

I am trying to clone a rice gene under a different endogenous rice promoter.I will be cloning the CDS of the rice gene.So I wanted to know what is the minimum or maximum distance I should put between ...
2
votes
0answers
23 views

Subtypes of Acute myeloid leukemia

I am a computer scientist with no biological background and working on analyzing lab results of patients with Acute myeloid leukemia. They have been tagged with following subtypes of AML: AML with ...
2
votes
0answers
34 views

Do bees make honey from fallen fruit?

I know bees make honey from nectar, and I know they drink the juice of fallen fruit, but do they actually make honey from fruit, or do they only eat from it for themselves?
2
votes
0answers
24 views

Assembly reconciliation

We have some bacterial genomes that were assembled using Spades, they were sequenced with and IonTorrent PGM. There are many assemblers and they give different results. I was interested in a tool ...
2
votes
0answers
30 views

Can ants learn things?

I have an ant nest in a jar. Whenever I want to observe them, I have to remove the cover and therefore put them in light - that scares them. They also can see shadow cast by myself. I often throw in ...

15 30 50 per page