3
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0answers
74 views

A day in Beijing is like smoking Only one sixth of a cigarette

This is the title of this blog post followed by this other good post. I'm particularly anti-tobacco-smoke, at the point of not always considering other pollution, and this article shows how this kind ...
3
votes
0answers
138 views

Gibson assembly using NeBuilder

I am supposed to construct a plasmid that contains features from two other plasmids. My strategy is to generate three fragments form the two other plasmids. I was encouraged to try Gibson assembly, ...
3
votes
0answers
222 views

Do Asian women have more difficult births than Europeans?

I recently heard a claim that Asians in general have more difficult births than Europeans. Is this true? I have found a few studies(1,2) on the effect of ethnicity on birth outcomes but they seem to ...
3
votes
0answers
84 views

Why don't surfactants destroy or incorporate into cell membranes?

How do surfactants remain on the surface of pneumocytes without (1) acting as a detergent or (2) the phospholipids getting incorporated into the membranes of pneumocytes... I'm guessing the answer ...
3
votes
0answers
25 views

What determines the thickness of Cornea?

In eye-operations such as Lasik and Femto, the thickness of cornea is key decision factor whether the operation is feasible. A normal thickness is between 500-600 µm. Cornea consists of a very special ...
3
votes
0answers
74 views

Could miniaturization be used to protect endangered large species?

In some animals (like dogs) size seems like it's controlled mostly by just a few genes (IGF1, and the genes that repress it). I'm curious: (1) Does other miniaturization takes a similar route (for ...
3
votes
0answers
48 views

Database of Medical Assertions

Can anyone point me toward a computationally-accessible database of medical assertions? I'm looking for something where each row in the database contains a single unit of knowledge. It could either be ...
3
votes
0answers
45 views

Why is this dry pepper fruit still “powered”?

Check out this photo of my pepper plant this cloudy morning: (click for full res) Usually (and this can be seen on 2 and a half of the 4 fruits in this pic) the fruits are "disconnected" from the ...
3
votes
0answers
38 views

Adenosine metabolism

Are adenosine or its catabolites increased in inflamed airways? How can I assess this? I am trying to use inhibitors for adenosine deaminase, xanthine oxidase, and purine nucleoside phosphorylase, but ...
3
votes
0answers
55 views

Nocioception (pain perception) in subcutaneous tissue

There seem to be no nocioceptors (pain and temperature sensitive nerve endings) in subcutaneous fat. there are nocioceptors and other receptors in veins running through fat, but these can be avoided. ...
3
votes
0answers
59 views

The Operation of tuning in the S1 layer of ventral model

According to my previous question in ventral Stream pathway and architecture, I want now to get a brief example about how the S1 layer is constructed. In other words, how all the simple units are ...
3
votes
0answers
342 views

Distinguishing between mitotic metaphase and metaphase II of meiosis

I'm sorry if this seems like a silly question, but I was brushing up on some cell cycle dynamics and realized that everyone was talking about the differences between metaphase I and II, and since ...
3
votes
0answers
30 views

Division of Polar Body

The first polar body formed after meiosis-I is completed in oocyte, may or may not divide. Why is the statement that "it may or may not divide"? Is it random ?
3
votes
0answers
62 views

Deducing synaptic strength from electron micrographs?

There are several promising techniques for connectomics based on iterative sectioning and imaging of tissue with scanning electron microscopes (e.g. FIB-SEM and ATUM) By looking at such micrographs, ...
3
votes
0answers
80 views

Can cats use ketone bodies as only source of energy?

I recall reading that much of the energy a cat produces from its food comes from proteins which I assume would produce energy via being catabolised into amino acids which in turn, if glucogenic, would ...
3
votes
0answers
318 views

Virus titre and copy number of integration

If I need to preform a genome-wide high throughput screen using pooled shRNA library infection followed by deconvolution by deep sequencing, how would I determine the optimal infection rate at which ...
3
votes
0answers
29 views

Are there any enzymes synthesised by humans that specifically catalyse the hydrolysis of non-cyclic Imides?

Imides or dicarbonyl amides are an interesting class of compounds that includes the pharmaceuticals thalidomide, aniracetam and a few other drugs. These compounds, however, are cyclic and I'm ...
3
votes
0answers
59 views

Is there a realtime molecular clock within the genome to co-ordinate the developmental sequences in an embryo?

It is difficult to assume that the massive number of co-ordinated developmental sequences in a developing embryo is controlled by molecular signalling alone. Is anyone aware of a molecular or ...
3
votes
0answers
68 views

Iron metabolism in the brain

I did an expression profiling from publicly available RNAseq data for mouse tissues. While for liver and testes I am getting expected proteins in the top highly expressed mRNAs, for brain I am getting ...
3
votes
0answers
124 views

Good poly-A filtering rules or tools

I am aligning a large number of ESTs. It seems poly-A tails show in many different ways. In addition to occurring at the very end, they can be flanked by the cloning sequence one one end, or have ...
3
votes
0answers
208 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
3
votes
0answers
90 views

Eye color genetics

I am trying to find a model to link the phenotype eye color to its genotypes. I know that there exists a simple model from Davenport, which explains {brown,blue} eyes. Further, there is an extended ...
3
votes
0answers
76 views

Food Intake versus ability to flee among birds, particularly the hummingbird?

Logically speaking, if a hummingbird drinks too much nectar, it will be temporarily overweight and less able or unable to fly to escape danger. However if the same hummingbird doesn't drink enough ...
3
votes
0answers
99 views

Why do ants dig surface paths?

A few days ago I observed a corridor built and used by ants that went over a path in fields: Why do they build it? It seems it doesn't bring any considerable protection from the surrounding ...
3
votes
0answers
39 views

Can I purify polyhydroxyalkanoates by heating the cells extensively?

Traditional methods of purifying polyhydroxyalkanoates (PHAs) and other bioplastics made by bacteria involve washing the cells with harsh chemicals or strong bases.I'm interested in maintaining the ...
3
votes
0answers
113 views

What is the mechanism by which Melatonin increases dream vividness and intensity in humans?

I'm looking at the wikipedia article on Melatonin and noticed that it mentions vivid dreaming caused by melatonin. I have the "Melatonin and the Biological clock" pamphlet referenced in the article, ...
3
votes
0answers
44 views

What processes are responsible for focusing on an image only seen by one of the eyes?

When a person is spying from around a corner with only one eye, it's rather easy to ignore the other eye's image, since it's probably much darker, because one usually spies around a corner standing ...
3
votes
0answers
56 views

What are polytene bands, and why are they there?

Drosophila polytene chromosomes have been particularly useful in genetic research, as it made cytogenetic gene mapping possible with very little effort. This was primarily accomplished due to polytene ...
3
votes
0answers
29 views

A program for cell motility assessment with a batch process function?

Cell motility assessment is a branch of experimental biology or medical science. One example could be an assessment of treatment effects on sperm motility of an animal. The standard procedure involves ...
3
votes
0answers
109 views

How does LCR compare to Assembly PCR

The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
3
votes
0answers
130 views

Is there a standard definition for plant “maturity”?

Is there a species-agnostic metric for identifying plant maturity? There seems to be plenty of literature defining life-history stages for specific crops, but it is not clear if there is a generic ...
3
votes
0answers
56 views

Can the Human body Synthesise Vitamin D from Phytosterols?

I know that the human body can synthesise vitamin D from cholesterol, but can it do so from phytosterols?
3
votes
0answers
1k views

What are the factors to consider when determining the sample size for a given study?

Say I'm designing a retrospective study (where I'd collect data from various schools for the last 5 years) to see if consuming milk can contribute to childhood obesity. How do I decide on how many ...
3
votes
0answers
167 views

Resuspending Cells from a filter plate

My experiment involves pulling down cells on to a filter plate for a variety of assays. However, I want to ensure that my cells are alive so I am trying to resuspend them so that I can do an accurate ...
3
votes
0answers
34 views

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments?

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments? Wikipedia says The actual role of bacteria in the low-temperature formation of ...
3
votes
0answers
44 views

Are all Autoreceptors Downregulated by their Respective Endogenous Ligands?

I know that the 5-HT1A and $\alpha$2 adrenoreceptors receptors serve as autoreceptors for serotonin (5-HT) and norepinephrine respectively and are down-regulated by repeat exposure to their respective ...
3
votes
0answers
73 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
3
votes
0answers
99 views

What are the physiological effects of wine?

I have read that alcohol in general and wine in particular can have advantageous as well as detrimental effects on human health. Is this true? What are the physiological effects of consuming wine in ...
3
votes
0answers
37 views

What Effect Does the Endocannabinoid System have on Monoaminergic Neurotransmission?

I know that Δ9-tetrahydrocannabinol and other cannabinoids increase dopaminergic neurotransmission in the nucleus accumbens and that this is what results in the euphoria experienced by cannabis users. ...
3
votes
0answers
24 views

Does Doxylamine have any Influence on the Monoaminergic System?

Does doxylamine have any affects on the monoaminergic system? Specifically I am interested in adrenergic or serotonergic activity but additional information on its dopaminergic activity won't hurt ...
3
votes
0answers
79 views

Experimental techniques to measure protein-protein and protein-ligand structure and dynamics

We would like to compare our simulation results with experimentation. When one wants to follow experimentally the dynamics (and possibly structure) of protein-protein or protein-ligand docking ...
3
votes
0answers
115 views

Cheapest Way to Measure Germ Density

I would like to measure the surface density of Staphylococcus bacteria and Rhinoviruses (only those two, to be specific) within my home. What's the cheapest way? The textbook procedure is to: rub ...
3
votes
0answers
124 views

RMSD during conformational transition in proteins

When I was investigating the differences between protein structures obtained by X-ray crystallography and NMR spectroscopy, I found the paper [1] compairing structures of several proteins resolved ...
3
votes
0answers
58 views

Physiological or molecular difference before and after sleep?

I know that the details of the process of sleep aren't entirely known, but have always wondered why I feel rested after sleep. Biologically, what differences can be directly observed in the human ...
3
votes
0answers
124 views

What controls the feeling of discomfort/comfort before and after sleep?

I'm interested in which biological systems or hormones are involved in the following phenomenon: Before sleep it may be difficult to find a comfortable position, and muscular aches and pains are more ...
3
votes
0answers
69 views

Are mature trees as susceptible to mutation from exposure to radiation as seedlings?

If a plant, say a ficus or a teak, as a sapling were exposed to radiation, and a tree of the same species were present at the same distance, which of the two would be more likely to mutate?
3
votes
0answers
162 views

Why are trichomes important for both the roots and shoots of plants?

In what ways are trichomes vital to the plant? Why have them on the plant shoots as well?
2
votes
0answers
19 views

How long do the periods of systole and diastole last?

The background to this is i'm doing some work using ECGs for an animation of the heart and i'm having trouble defining the end points of these periods, particularly diastole. Currently, the only ...
2
votes
0answers
17 views

What is this frog?

(Man, there's a lot of 'What is this X?' questions here...) I saw this while walking in Cyprus in early April. It was at about 100m altitude, in woodlands, sitting in the sun on a concrete step ...
2
votes
0answers
11 views

Is emergence connected to nature's transition between fractal regimes at different size scales?

A fractal algorithm like Mandelbrot is self-similar in all size scales. This is not the case in nature. A tree is fractal in the sense that each branch is similar to the tree as a whole. But that is ...

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