3
votes
0answers
47 views

Database of Medical Assertions

Can anyone point me toward a computationally-accessible database of medical assertions? I'm looking for something where each row in the database contains a single unit of knowledge. It could either be ...
3
votes
0answers
45 views

Why is this dry pepper fruit still “powered”?

Check out this photo of my pepper plant this cloudy morning: (click for full res) Usually (and this can be seen on 2 and a half of the 4 fruits in this pic) the fruits are "disconnected" from the ...
3
votes
0answers
38 views

Adenosine metabolism

Are adenosine or its catabolites increased in inflamed airways? How can I assess this? I am trying to use inhibitors for adenosine deaminase, xanthine oxidase, and purine nucleoside phosphorylase, but ...
3
votes
0answers
59 views

The Operation of tuning in the S1 layer of ventral model

According to my previous question in ventral Stream pathway and architecture, I want now to get a brief example about how the S1 layer is constructed. In other words, how all the simple units are ...
3
votes
0answers
30 views

Division of Polar Body

The first polar body formed after meiosis-I is completed in oocyte, may or may not divide. Why is the statement that "it may or may not divide"? Is it random ?
3
votes
0answers
61 views

Deducing synaptic strength from electron micrographs?

There are several promising techniques for connectomics based on iterative sectioning and imaging of tissue with scanning electron microscopes (e.g. FIB-SEM and ATUM) By looking at such micrographs, ...
3
votes
0answers
79 views

Can cats use ketone bodies as only source of energy?

I recall reading that much of the energy a cat produces from its food comes from proteins which I assume would produce energy via being catabolised into amino acids which in turn, if glucogenic, would ...
3
votes
0answers
301 views

Virus titre and copy number of integration

If I need to preform a genome-wide high throughput screen using pooled shRNA library infection followed by deconvolution by deep sequencing, how would I determine the optimal infection rate at which ...
3
votes
0answers
29 views

Are there any enzymes synthesised by humans that specifically catalyse the hydrolysis of non-cyclic Imides?

Imides or dicarbonyl amides are an interesting class of compounds that includes the pharmaceuticals thalidomide, aniracetam and a few other drugs. These compounds, however, are cyclic and I'm ...
3
votes
0answers
68 views

Iron metabolism in the brain

I did an expression profiling from publicly available RNAseq data for mouse tissues. While for liver and testes I am getting expected proteins in the top highly expressed mRNAs, for brain I am getting ...
3
votes
0answers
200 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
3
votes
0answers
76 views

Food Intake versus ability to flee among birds, particularly the hummingbird?

Logically speaking, if a hummingbird drinks too much nectar, it will be temporarily overweight and less able or unable to fly to escape danger. However if the same hummingbird doesn't drink enough ...
3
votes
0answers
97 views

Why do ants dig surface paths?

A few days ago I observed a corridor built and used by ants that went over a path in fields: Why do they build it? It seems it doesn't bring any considerable protection from the surrounding ...
3
votes
0answers
39 views

Can I purify polyhydroxyalkanoates by heating the cells extensively?

Traditional methods of purifying polyhydroxyalkanoates (PHAs) and other bioplastics made by bacteria involve washing the cells with harsh chemicals or strong bases.I'm interested in maintaining the ...
3
votes
0answers
111 views

What is the mechanism by which Melatonin increases dream vividness and intensity in humans?

I'm looking at the wikipedia article on Melatonin and noticed that it mentions vivid dreaming caused by melatonin. I have the "Melatonin and the Biological clock" pamphlet referenced in the article, ...
3
votes
0answers
44 views

What processes are responsible for focusing on an image only seen by one of the eyes?

When a person is spying from around a corner with only one eye, it's rather easy to ignore the other eye's image, since it's probably much darker, because one usually spies around a corner standing ...
3
votes
0answers
56 views

What are polytene bands, and why are they there?

Drosophila polytene chromosomes have been particularly useful in genetic research, as it made cytogenetic gene mapping possible with very little effort. This was primarily accomplished due to polytene ...
3
votes
0answers
29 views

A program for cell motility assessment with a batch process function?

Cell motility assessment is a branch of experimental biology or medical science. One example could be an assessment of treatment effects on sperm motility of an animal. The standard procedure involves ...
3
votes
0answers
103 views

How does LCR compare to Assembly PCR

The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
3
votes
0answers
125 views

Is there a standard definition for plant “maturity”?

Is there a species-agnostic metric for identifying plant maturity? There seems to be plenty of literature defining life-history stages for specific crops, but it is not clear if there is a generic ...
3
votes
0answers
54 views

Can the Human body Synthesise Vitamin D from Phytosterols?

I know that the human body can synthesise vitamin D from cholesterol, but can it do so from phytosterols?
3
votes
0answers
987 views

What are the factors to consider when determining the sample size for a given study?

Say I'm designing a retrospective study (where I'd collect data from various schools for the last 5 years) to see if consuming milk can contribute to childhood obesity. How do I decide on how many ...
3
votes
0answers
34 views

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments?

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments? Wikipedia says The actual role of bacteria in the low-temperature formation of ...
3
votes
0answers
43 views

Are all Autoreceptors Downregulated by their Respective Endogenous Ligands?

I know that the 5-HT1A and $\alpha$2 adrenoreceptors receptors serve as autoreceptors for serotonin (5-HT) and norepinephrine respectively and are down-regulated by repeat exposure to their respective ...
3
votes
0answers
73 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
3
votes
0answers
97 views

What are the physiological effects of wine?

I have read that alcohol in general and wine in particular can have advantageous as well as detrimental effects on human health. Is this true? What are the physiological effects of consuming wine in ...
3
votes
0answers
35 views

What Effect Does the Endocannabinoid System have on Monoaminergic Neurotransmission?

I know that Δ9-tetrahydrocannabinol and other cannabinoids increase dopaminergic neurotransmission in the nucleus accumbens and that this is what results in the euphoria experienced by cannabis users. ...
3
votes
0answers
23 views

Does Doxylamine have any Influence on the Monoaminergic System?

Does doxylamine have any affects on the monoaminergic system? Specifically I am interested in adrenergic or serotonergic activity but additional information on its dopaminergic activity won't hurt ...
3
votes
0answers
79 views

Experimental techniques to measure protein-protein and protein-ligand structure and dynamics

We would like to compare our simulation results with experimentation. When one wants to follow experimentally the dynamics (and possibly structure) of protein-protein or protein-ligand docking ...
3
votes
0answers
115 views

Cheapest Way to Measure Germ Density

I would like to measure the surface density of Staphylococcus bacteria and Rhinoviruses (only those two, to be specific) within my home. What's the cheapest way? The textbook procedure is to: rub ...
3
votes
0answers
122 views

RMSD during conformational transition in proteins

When I was investigating the differences between protein structures obtained by X-ray crystallography and NMR spectroscopy, I found the paper [1] compairing structures of several proteins resolved ...
3
votes
0answers
57 views

Physiological or molecular difference before and after sleep?

I know that the details of the process of sleep aren't entirely known, but have always wondered why I feel rested after sleep. Biologically, what differences can be directly observed in the human ...
3
votes
0answers
123 views

What controls the feeling of discomfort/comfort before and after sleep?

I'm interested in which biological systems or hormones are involved in the following phenomenon: Before sleep it may be difficult to find a comfortable position, and muscular aches and pains are more ...
3
votes
0answers
68 views

Are mature trees as susceptible to mutation from exposure to radiation as seedlings?

If a plant, say a ficus or a teak, as a sapling were exposed to radiation, and a tree of the same species were present at the same distance, which of the two would be more likely to mutate?
3
votes
0answers
159 views

Why are trichomes important for both the roots and shoots of plants?

In what ways are trichomes vital to the plant? Why have them on the plant shoots as well?
2
votes
0answers
13 views

How to quantify the Abundance of a particular gene in Bacteria and Archaea?

For 16s rDNA metagenomic analysis, do I need to do the following steps? DNA extraction-> Purification-> PCR amplification-> Clone -> Pick every single colony-> PCR amplify -> Sequence -> ...
2
votes
0answers
20 views

Membrane potential in gram negative bacteria

Does the membrane potential usually quoted for gram negative bacteria (e.g. E. coli) refer to the potential across both membranes? - If yes, then does the potential fall more over the inner or outer ...
2
votes
0answers
19 views

Can encoding of miRNAs in introns not cause dysregulation of gene expression?

For example, consider a gene X which has an intron which encodes a miRNA that downregulates expression of another gene Y. When the gene X is translated, this miRNA will appear as a byproduct of ...
2
votes
0answers
25 views

Contact Inhibition of Cell Division: Signaling Pathway

The following article refers to contact inhibition of cell division in epithelial cells, specifically MDCK cells: Collective and single cell behavior in epithelial contact inhibition. In their review ...
2
votes
0answers
33 views

How to get only perfect matches with blastn?

I have installed Blast locally and I've configured the nucleotide database to use. I want to search some sequences (from a .fastafile) in this genome. So I used blastn: ...
2
votes
0answers
23 views

Finding confidence level of miRNA disease associations

I'm an undergraduate computer engineering student, and I have a project about bioinformatics. In this manner, I need to find prediction( or association I'm not sure the correct terminology) confidence ...
2
votes
0answers
29 views

DNA-DNA cross-linking with formaldehyde?

The 3C (chromosome conformation capture) technology for studying chromatin 3D organization starts by a cross-linking step using formaldehyde to find segments of DNA interacting. In my understanding ...
2
votes
0answers
30 views

Are there sterilisation methods that do not denature proteins as heat does?

Context: Most countries require milk to be sterilised through radiation or heat to remove possible harmful bacteria. Both of these processes denature the proteins in the milk (ref). Are there methods ...
2
votes
0answers
16 views

What is the role of RAGEs?

According to articles I read, AGEs (advanced glycation end products) activate RAGEs (receptors for AGEs). This activation increases the ROS (reactive oxygen species) levels in the cells. 2003 - ...
2
votes
0answers
19 views

Atelectesis due to decresed surfactant in lungs

Surfactant is a protein lipid mixture produced by alveolar pneumocytes composed of Dipalmitoyl Phosphatidly Choline lipid,apoproteins and calcium ions. This surface lines the alveolar epithelium ...
2
votes
0answers
31 views

What is NK-cell compartments?

with respect to the paper: Adaptive reconfiguration of the human NK-cell compartment in response to cytomegalovirus: A different perspective of the host-pathogen interaction What is meant by ...
2
votes
0answers
27 views

How does de-myelination occur in multiple sclerosis?

From what I understand, only the oligodendrocytes are affected in multiple sclerosis, and they are attacked by T cells which cross the blood-brain barrier. This leads me to two questions: How is the ...
2
votes
0answers
5 views

MTHFR recessive alleles and B-vitamins

I know that being heterozygous or homozygous recessive for the MTHFR gene prevents that person from being able to process and use folate and B-12. It causes an unhealthy level of homocysteine to ...
2
votes
0answers
36 views

Sensitivity of eyes to flashing lights

My eyes are very sensitive to flashing lights - for example I'm always the first person to notice that a fluorescent tube is about to fail because I see it flickering when other people can't. When ...
2
votes
0answers
21 views

Degree of dosage compensation in Drosophila melanogaster

In this paper the authors state that the dosage compensation seen in Drosophila is approximately twofold, but they do not provide any source or numbers (as far as I can see) for this. What is the mean ...

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