2
votes
0answers
20 views

What is the role of RAGEs?

According to articles I read, AGEs (advanced glycation end products) activate RAGEs (receptors for AGEs). This activation increases the ROS (reactive oxygen species) levels in the cells. 2003 - ...
7
votes
2answers
113 views

Do butterflies see behind?

Butterflies, like many other insects, understand that I am getting near from behind. How do they feel this - through vision, hearing, or some other sense?
1
vote
0answers
19 views

Is (are) there any crucial gene(s) for the formation of flower in flowering plants? [closed]

I am interested in qualitative (flowers of some plants have petal or sepal, but some plants have not) and quantitative (number of flowers of plants) differences between flowers of different plants. ...
3
votes
1answer
107 views

What type of flask should I use to culture NTERA2 embryonic cancer stem cells?

I'm just starting my MSc research and I am in the process of making a list of equipments/consumables to order. Is there a specific flask in which I can culture NTERA2 (NTERA2/D1) cell line?
1
vote
3answers
262 views

When is the second polar body extruded from the egg nucleus?

When does the second polar body gets expelled from the egg nucleus during oogenesis ? I know that it occurs after the entry of the sperm into the secondary oocyte but does it occur before the ...
5
votes
1answer
62 views

Is hands-only CPR as effective as traditional CPR?

Is hands-only CPR as effective as traditional CPR ? Wouldn't 30 chest compressions + 2 rescue breaths be more effective if the person doesn't have a pulse AND not breathing? From my understanding ...
1
vote
1answer
41 views

Yeast absorption of different spectra of light

I'd like to monitor the growth of a yeast population by shining light through it and monitoring the change in light that gets through as it grows. I'm not sure if I should use IR, or any particular ...
4
votes
2answers
58 views

How can (or did) Deinococcus radiodurans continue to evolve after developing resistance to mutation?

Deinococcus radiodurans has a remarkable ability to resist damage to its DNA due to radiation, dehydration or (to my knowledge) any other source. It keeps multiple copies of its genome and has a ...
0
votes
1answer
98 views

What are genome wide functional linkages?

There are two types of interaction classifications used to describe Protein-protein interactions, namely physical and functional. Whilst physical interactions are obvious in nature and methodological ...
12
votes
2answers
337 views

Do transcription factors bind to both strands of DNA?

Do transcription factors (or generally proteins) bind to only single strand of DNA or both strands? Since it can have non covalent bonds to both strands in theory. I would like to know the mechanism. ...
7
votes
1answer
67 views

Do DNA repressors exist?

I know about enhancers and the mechanism that lead them to increase the gene expression of their targets but I was wondering if similarly DNA repressors exist. I know about protein repressors but I am ...
6
votes
1answer
68 views

“Enhancers” of enhancers?

I am looking for examples (if any) of genomic regions which regulates the activity of enhancers, either augmenting or reducing it. Essentially some kind of enhancers (or repressors) of enhancers to ...
6
votes
2answers
350 views

What physiological processes within a muscle cause the latent period in a muscle fiber?

This is a lab question I cant find the answer in the lab manual or in the text book. What physiological processes in a muscle cause the latent period in a muscle fiber? Please help. Edit: The latent ...
0
votes
2answers
58 views

books for beginners in research in mathematical biology [closed]

I am a M.SC(mathematics) student. I have no subject like mathematically modelling.But i'm interesting in research in mathematical biology.Initially which book i should study. Please guide ...
4
votes
2answers
72 views

Questions on DNA damage

I'm not strong in biology, so bear with me on this: I've been reading that as we age, our DNA is damaged by internal (e.g. errors during replication) and external (e.g. sun damage or radiation) ...
2
votes
1answer
60 views

How did bullying arise evolutionarily?

By bullying, I mean individuals harassing others with name-calling or violence but not for the purpose of gaining resources such as with extortion or theft. The only explanation I've thought of is ...
8
votes
1answer
145 views

What sample sizes are ideal for carrying out Bayesian Skyline Plots?

I am interested in creating Bayesian Skyline Plots to look at demographic changes in certain population groups. However, these populations have very little within population variation. Around 5 ...
5
votes
2answers
60 views

Help with STRING database data

I'm working with data downloaded from STRING database (string-db.org) for protein-protein interactions. My idea is to compare the topology of connections of the same protein on different organisms. ...
5
votes
2answers
91 views

What happens during kefir fermentation process?

I’ve found many sources about the positive effects of kefir for the digestive system. However I haven’t found any information about the fermenting process. What is the exact biology (chemistry?) ...
12
votes
2answers
451 views

What is the explanation for the smaller number of tRNA than codons?

Translation, or decoding, of the four-nucleotide language of DNA and mRNA into the 20–amino acid language of proteins requires tRNAs and enzymes called aminoacyl-tRNA synthetases. To ...
5
votes
1answer
218 views

Why is E.coli used as a model?

Is there a reason for the choice of E.coli as a model for many bacterial systems? Other bacteria such as B.subtilis are also used, but why is E. coli preferred?
1
vote
1answer
24 views

How to correlate the pattern by which CAP activator from E.coli binds to DNA and its mechanism of action?

The catabolite activator protein (CAP) activates the expression of more than 100 genes involved in secondary sugar metabolism in E.coli. Apparently, it always binds in sites that are away from -10 and ...
3
votes
1answer
99 views

What causes the line patterns in the palm of the hand?

The line patterns on the hand are unique to each individual, but what causes these lines and re they advantageous in anyway?
3
votes
2answers
210 views

Is there a specific mechanism for the delivery of pain medication?

For example, when one takes aspirin or ibuprofen does the chemical get dispersed to all pain receptor? My question really is, how does the chemical know where to target in the body? I figure wherever ...
4
votes
1answer
82 views

Is there a DNA analogue to ribozymes? [duplicate]

If not, is it impossible for DNA to have enzymatic activity?
1
vote
1answer
38 views

How long the homologous arms of the “repair” sequence must be to do homologous recombination? [duplicate]

I am trying to figure out the details of the homolougous recombination in order to be able to insert a certain piece of DNA in the host genome after double strand break.. Does anybody know how long ...
3
votes
1answer
105 views

How would you affect bulk DNA gene therapy for a human?

Let's imagine that we understood DNA programming and our genome very well and realized that there were some significant flaws (we die, we need sleep, etc.) And let's imagine that we understand how to ...
7
votes
3answers
61 views

Mechanism by which $lacI^{d}$ is a dominant mutation, impairing the function of normal copies of the Lac Repressor

Jacob-Monod model for the lac Operon was based on experiments using two strands of bacteria which constitutively expressed $\beta$-gal: $I^{c}$(mutation in the gene lacI , which encodes the repressor) ...
0
votes
1answer
57 views

Incubation time of Antarctic Phosphatase in lab [closed]

Antarctic Phosphatase is used to catalyze the removal of 5' phosphate from DNA. If I'm using it in the lab on a sample of 1-5 micrograms of digested DNA, how would I figure out how long to incubate ...
5
votes
1answer
148 views

Is consuming proteins different vs. consuming amino-acids and how?

Yesterday I had a discussion with a friend. He said that consuming proteins and amino-acids is different. He said that those who grow muscles would agree on that. I wanted to argue against that ...
2
votes
1answer
46 views

Atelectasis due to decreased surfactant in lungs

Surfactant is a protein lipid mixture produced by alveolar pneumocytes composed of Dipalmitoyl Phosphatidyl Choline lipid, apoproteins and calcium ions. This surface lines the alveolar epithelium ...
8
votes
3answers
82 views

What triggers the migration of robins?

I live in central Massachusetts, and have begun seeing robins, as we generally do in early March. The temperature is well below normal, though, and three feet of snow are covering the still-frozen ...
5
votes
2answers
193 views

What is the IC50 exactly?

I am reading the paper "Activity of the bcr-abl kinase inhibitor PD180970" but I don't understand how IC50 works on table 1. Can you tell with simple words and give me an easy example?
0
votes
0answers
24 views

Why is acid alcohol used as a decorizer in the acid fast stain procedure, as opposed to a neutral alcohol?

Is it to neutralize the negative components of the cell membrane, thus preventing the positively charged dye from adhering to the cell membrane?
9
votes
1answer
171 views

RNA migrating slower than DNA on Formaldehyde Gel?

So I ran into an interesting problem. I'm getting a linear DNA band that is twice as long (4x bases, but as denatured probably only 2x) as an RNA band running at the same size in a formaldehyde gel. ...
1
vote
0answers
24 views

Why do contrasts during CT scans make the body feel warm? [closed]

Why do the contrasts used during CT scans make the body feel hot/warm? I think they use iodine, so why would the bodies response to iodine be a warm sensation? In addition, I think they make one's ...
3
votes
1answer
34 views

Why is knowledge of bacterial pathogenesis important? [closed]

Why is knowledge of pathogenesis increased more and more for common bacterial pathogens? Most of these studies lack a near application in disease control such as vaccines or antibiotics. Examples: ...
3
votes
1answer
70 views

Is it possible to insert DNA without cutting the recognition site with CRISPR/Cas9?

We are looking for a way to insert DNA into a genome, but we would like to do it in a way that the recognition site stay intact to be able to add again DNA at the same location. Do you know if it is ...
5
votes
1answer
81 views

Doubt on genomic code for nucleosome positioning?

I was reading "A genomic code for nucleosome positioning" (by Eran Segal et al). And I am having 2 doubts. The figure(b) in this image from the paper shows the graph of fraction (3-bp moving ...
4
votes
1answer
72 views

DNA sequencing problem

First off, let me start by outlining the problem: Your laboratory has established a technique for examining DNA replication in a cellular extract. To the cellular protein extract, you add ...
5
votes
1answer
115 views

Can human mRNA be translated in vitro by prokaryotes?

As the genetic code is universal, can mRNA from a human cell be correctly translated by a prokaryote in a in vitro translation system?
2
votes
1answer
71 views

How does alternative splicing work?

I am trying to find out what controls what exons are spliced out, and I keep coming across the term cis regulator, but I cannot seem to find a clear explanation of what happens... Thank you in ...
0
votes
1answer
117 views

What are microRNA, siRNA and antisense RNA?

From what I understand, microRNA binds to proteins which can cut certain mRNA strands do that this protein is not synthesised. This seems like gene silencing to me, however I have also come across the ...
1
vote
1answer
22 views

RNA-Polymerase III transcribed genes

I have a list of differentially regulated genes from a high-throughput experiment of the human genome. I would like to find out which of these genes are transcribed by a RNA polymerase III and which ...
4
votes
2answers
653 views

What's a protein pulldown assay?

I'm reading a paper and the authors mention a "protein pulldown" assay. I've never done this before, and googling doesn't bring up much. Could I get a rundown of the basic theory behind it? Also ...
1
vote
1answer
27 views

What are the roles of cadherins in epithelial mesenchymal transitions? [closed]

I know that cadherins control the expression of cancer cells involved in the epithelial-mesenchymal transitions, but I was wondering exactly how the process worked.
2
votes
1answer
61 views

Relationship between leaf structure and Photosynthesis rate

We can see numerous leaf structures in plant kingdom. As the leaves are designed for photosynthesis, their structure must be a factor for any optimization in photosynthesis or chlorophyll ...
4
votes
1answer
82 views

Nomenclature of genes and proteins

With respect to this paper: Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen In the abstract, I found names such as Rab6 and Vps35 and TNPO3. So Rab6 ...
2
votes
1answer
24 views

Introducing novel genes to an adult multicellular organism?

Is it possible to introduce a novel gene into the genome of every cell (or at least most cells) of an adult multicellular organism? How would this be done? Thanks, CDB
0
votes
1answer
65 views

What, if any, diseases do larder beetles carry?

I found one of these beetles on my bed as I was attempting to get in. I'm 80% sure my cat carried it in (I found it in the exact same space he had been sitting ten minutes previously). My question is ...

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