1
vote
1answer
34 views

Serious question about a piece of meat

Does anyone know some scientific rigorous way to look at a slice of meat. I am not asking what kind of meat it is or which part of the animal it came from. I wonder what is the different lining on ...
1
vote
1answer
28 views

What factors, other than its homochirality, make our “brand” of biology unique? [closed]

If humans were to discover organisms on another planet, it is supposed that (unless both we and they were seeded by the same source) we would have nothing to fear from alien pathogens, as they would ...
2
votes
1answer
21 views

Why are skin tactile receptors considered to be phasic receptors?

A phasic receptor by definition is one that quickly adapts to change. In terms of neuron firing, this means that it does not trigger another firing because the receptors are used to the stimulus. But ...
-2
votes
0answers
20 views

Punnet Square help [closed]

Just wanting to make sure I am doing this correctly. Given: R dominant allele for able to roll tongue r not able to roll tongue T dominant allele for taster of PTC t not able to ...
21
votes
6answers
5k views

Why is cold water more refreshing than warm water?

Obviously, the temperature of water does not affect its chemical composition. At least not in the ranges we are likely to drink it in. Yet it is clearly far more pleasant and refreshing to drink cool ...
1
vote
1answer
33 views

Does DNA Ligase look for complementarity in sticky ends?

Can DNA ligase seal two non-complementary sticky ends if the reaction is incubated at the optimum conditions ? I do almost 100% double digestion in the lab. However, during ligation, when I do a ...
9
votes
1answer
80 views

RNA migrating slower than DNA on Formaldehyde Gel?

So I ran into an interesting problem. I'm getting a linear DNA band that is twice as long (4x bases, but as denatured probably only 2x) as an RNA band running at the same size in a formaldehyde gel. ...
2
votes
0answers
50 views

Is the “concept of time” coded into the genome? [closed]

What I'm interested about is whether environmental changes' effects are coded into the genome, like metamorphosis, deciduous plants. Can it be explained by morphogens, or it's something completely ...
1
vote
0answers
21 views

How to gauge the clinical significance of specific cell type presence?

How does one decide whether the presence of certain cell types is clinically important or negligible? Would the presence of certain cells in conjunction with other symptoms be enough, or should it be ...
3
votes
1answer
26 views

Is there a genomic code for nucleosome positioning?

What does a genomic code for nucleosome positioning in eukaryotes actually mean? By the code is it right to think that specific DNA sequences favour nucleosomes and others don't? I see that there for ...
0
votes
0answers
42 views

Medical Uses of toxic venom

One interesting thing I recently learned is that venom has medical uses that can actually save lives! But from what I see so far this either applies to venoms from creatures that are not fatal to ...
9
votes
3answers
562 views

Why are fruits so large compared to their seeds?

Why do many plants produce such large fruits(apples and strawberries,for example) if those contain only relatively small seeds?
-1
votes
0answers
31 views

What is the difference between caspase and DNase? [closed]

Could somebody tell me what the difference between caspase and DNase is?
2
votes
1answer
141 views

When contracting a muscle will the muscle spindle stretch or contract?

When you contract your muscle, will your muscle spindle stretch or contract? And why? I was always under the impression that it was contracting your muscle spindle, but now I am not sure.
5
votes
1answer
52 views

Is using Hidden Markov Models to find homologues sensible in abstract, short sequences?

HMM alignment tools like hhpred excel at finding subtle homologues of folded proteins that simpler scoring techniques (such those used in BLAST algorithms) would miss. I am only looking at a small ...
0
votes
1answer
1k views

What determines the size and shape of a cell? [closed]

What determines the size and shape of a cell? They differ at different tissues / organs/ species
5
votes
1answer
95 views

Congenital blindness due to retinitis pigmentosa - does it exist?

Retinitis pigmentosa (RP) patients typically become blind after a period of years in which their eye sight slowly deteriorates due to photoreceptor degeneration. Generally RP patients develop ...
2
votes
0answers
23 views

Finding confidence level of miRNA disease associations

I'm an undergraduate computer engineering student, and I have a project about bioinformatics. In this manner, I need to find prediction( or association I'm not sure the correct terminology) confidence ...
2
votes
1answer
31 views

What is the difference between myopia and hyperopia?

In myopia the lens is bending the light too much even in the most relaxed state. Thus the image is produced in front of the retina. If the object comes closer the lens just adjusts a bit less than a ...
6
votes
1answer
143 views

How is color information transmitted from the eye to the brain?

Is color information sent from the eye to the brain frequency-modulated, or are different colors transmitted by different axons? I know that each ganglion cell is connected to multiple ...
5
votes
1answer
47 views

What is the mechanism behind tinnitus?

I seem to have come across two contrasting explanations for tinnitus induced by loud noises- i.e. damage to the hair cells in the cochlea. On the one hand, I have read that damage to the hair cells ...
3
votes
2answers
31 views

Why can we use mouse-produced antibodies on mice tissues?

I have seen biologists use mouse grown primary antibodies in mouse tissue, and they've told me that if the blood is perfused well then there is no problem with this method. How does the secondary ...
2
votes
0answers
30 views

Are there sterilisation methods that do not denature proteins as heat does?

Context: Most countries require milk to be sterilised through radiation or heat to remove possible harmful bacteria. Both of these processes denature the proteins in the milk (ref). Are there methods ...
3
votes
0answers
21 views

Can't resolve protein with native PAGE

This is a native gel. Let's call the left 2 lanes protein A and the right 2 lanes protein B. B is the same as A except it has a FLAG tag. They are both homotetramers of about 65 kDa. After ...
3
votes
1answer
20 views

Oligonucleotide purification with desalting

I have ordered 36bp oligonucleotides that anneal to each other and create sticky ends to be cloned in a vector afterwards. I have tried cloning many times with different methods and I failed. Now I ...
0
votes
0answers
14 views

How are Stem Cells Produced for Stem Cell Based Therapies?

I was doing an initial search on google but couldn't come up with anything I'm looking for. I know were stem cells come from and a bit about their levels of differentiation. For example, embryonic ...
-1
votes
0answers
37 views

Is technological intelligence a trait not important for survival given that it evolved only once (humans)? [closed]

And by technological intelligence I mean the ability to write down information and pass it along to children that would be able to use it in whichever way. This is probably what distinguishes us from ...
3
votes
1answer
64 views

Nomenclature of genes and proteins

With respect to this paper: Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen In the abstract, I found names such as Rab6 and Vps35 and TNPO3. So Rab6 ...
0
votes
3answers
36 views

Why autosomal trisomy has more adverse effects than sex choromosome monosomy or trisomy?

Autosomal Trisomy syndromes are usually lethal but sex chromosome number related abnormalities do not lead to that much fatality. why?
3
votes
1answer
21 views

Obvious phenotypic inheritance markers in humans

In humans, are there phenotypic markers that prove parenthood? For example, if mother and father have <trait characteristic> (let's say, specific ear lobe ...
8
votes
1answer
9k views

how to close a severed artery/vein?

This is a medicine-related question that I've been wondering about. I've been watching medical lectures on YouTube regarding the human body and was wondering about how surgeons treat a severed artery ...
1
vote
0answers
24 views

Should gene therapy safety protocol include isolation?

In the case of a gene therapy trial where viral vectors are used to deliver genes into mammalian cells, including humans, should biosafety and ethical protocols include isolation of the patient as a ...
2
votes
0answers
30 views

DNA-DNA cross-linking with formaldehyde?

The 3C (chromosome conformation capture) technology for studying chromatin 3D organization starts by a cross-linking step using formaldehyde to find segments of DNA interacting. In my understanding ...
6
votes
1answer
151 views

Grapefruits and CYP3A4

Grapefruit juice contains furanocoumarins, which irreversibly inhibit CYP3A4. For this reason, when one is taking certain medications it is necessary to not eat grapefruits because the inhibition of ...
0
votes
1answer
17 views

What is an apomorphy? What is a plesiomorphy? Can someone confirm these definitions?

I want to know the correct definitions for the terms apomorphy and plesiomorphy. Unfortunately, there seems to be a variety of ways to define these terms depending on the language one uses. Thus, it ...
2
votes
1answer
24 views

What does these $\Delta \Delta G$ numbers signify?

I was reading a paper and came across this table showing $ \Delta \Delta G$ numbers of different nucleotide sequences in DNA/RNA. I know that $\Delta G$ is free energy and $ \Delta \Delta G$ is ...
1
vote
1answer
34 views

Blast output - program to count the number of hits [duplicate]

I have to use the old version of Blast (not Blast+) to compare DNA sequences and I have to know the number of all hits. They are not given in the header and I have to know them for many many of blast ...
1
vote
0answers
58 views

How did birds become so intelligent?

I have seen a Discovery Channel video of a bird throwing stones in a pot to raise the water level so it is able to reach and drink it. A similar experiment in this paper from Current Biology shows ...
0
votes
0answers
20 views

Founders of Cnidarian colony

I know that colonial Cnidaria enlarge their colony by the means of asexual reproduction and sexual and asexual reproduction can originate individuals that will be part of other colonies. ...
2
votes
1answer
14 views

Does mung bean nuclease cleave a phosphate group when it's chewing off 5' or 3' ssDNA ends?

I'm looking to create blunt ends from sticky ends with mung bean nuclease for subsequent ligation. Does anyone know full mechanism by which mung bean nuclease will do this? In particular after the ...
4
votes
1answer
34 views

How can I reduce the background gradient in phase contrast microscopy?

I'm looking for ways to set up a phase contrast microscope to reduce the brightness gradient that appears across the background of my images. I use phase contrast to find the outlines of cells in my ...
3
votes
1answer
31 views

Why do mitochondria fuse together?

Contrary to all of the textbook images of mitochondria that I have seen over the years, I had just learned that the mitochondria within a cell form a dynamic branching network along microtubule ...
3
votes
2answers
65 views

Why can't the human eye focus blue light?

I recently noticed that it is hard to focus on blue light sources, especially at night. When observing a blue light source, e.g. a neon sign, it looks somewhat blurry. A sign with a different colour ...
3
votes
0answers
56 views

Are there any de-novo genome assembly programs for HADOOP?

I have access to a small (approx 20 nodes) cluster that has HADOOP running on it, and I need to do some de-novo genome assembly. I'd like to ask is anyone knows of any program that can do this on a ...
3
votes
2answers
41 views

Are restriction enzymes active at −20 °C?

I have digested my DNA with NotI enzyme and put it in the −20 °C freezer without heat inactivating it. Can restriction enzymes work at −20 °C? Should I expect STAR activity?
7
votes
1answer
85 views

Why do some vaccines last longer than others?

After reading an answer to the question of How Do White Blood Cells Learn? Or Do They?, I came to wonder something. Specifically, The effect of this is that every new B and T cell that your bone ...
6
votes
2answers
44 views

Can molecular genetics make a boolean variable from a continuous variable?

In the same kind of idea than this question. Gene expression are regulated through complex interactions. The concentration of enhancers and repressors is an important aspect that dictate the level of ...
2
votes
2answers
68 views

Local BLAST Copy Number per Hit

I generated a series of local BLAST databases using makeblastdb of metagenomic data and am searching for the presence of a particular gene. While I can do the normal BLAST analysis looking at ...
5
votes
2answers
94 views

Are unilaterally deaf people able to determine where sound comes from?

My question is on people deafened in one ear, but normal hearing in the other. Time and level differences between the two ears are only part of how the human body can localize the source of the ...
2
votes
2answers
3k views

What is a pA(2) value?

I saw this article http://www.ncbi.nlm.nih.gov/pubmed/16710314 and it mentioned pA(2) values and I had no idea what they were. What are they? What do they mean? If possible it'd be just dandy if you ...

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