All Questions

0
votes
0answers
72 views

Knowledge about Organelles [closed]

As we know, there are various organelles present inside a cell. Let's consider mitochondria for example. My questions are -> Do we understand the composition, structural and functional features of ...
4
votes
2answers
45 views

Is there a command line molecular cloning tool?

There are many softwares such as SnapGene, Geneious, Vector NTI, ApE, Gentle and others for assistance with cloning. Their key functions are: Making maps of plasmids Annotating plasmids with common ...
-2
votes
0answers
18 views

left to right hand eidetic memory affection [closed]

If a child who was left handed is made right handed, will it affect his eidetic memory ? I need to know please. No information from other sources. I tried
0
votes
0answers
24 views

Would a 3d monitor reduce eye strain from daily computer work? [closed]

I'm really not sure which SE community to post this to, but it seems most appropriate here... I work as a web developer. For 8-12 hours per day I stare at code 12 inches in front of my face. Beyond ...
1
vote
0answers
21 views

How does proximal centriole of human sperm function?

I've read that the human's unfertilized oocyte ( stuck at metaphase 2 of meiosis ) lacks centrioles and that it completes meiosis 2 after fertilization as sperm gives its proximal centriole. Then what ...
18
votes
4answers
1k views

Why do we need red blood cells?

From what I know, the main function of red blood cells is hemoglobin transport. So, why do we need cells packed with hemoglobin: why can't it travel freely in the bloodstream? My own thoughts were: ...
9
votes
2answers
580 views

Which sex has higher variance of reproductive rate in modern societies - male or females?

Who has a more varied reproduction rate in modern western societies - men or women? The average rate is the same of course, but I wonder which sex have higher variance - higher variance means that ...
1
vote
0answers
24 views

Loss in DNA band size extracted from polyacrylamide gel

I'm using cDNA AFLP teq.. I extracted 670 bp band from polyacrylamide (urea) gel and I have amplified with PCR but on agarose gel it stops at nearly 350 bp.it happened for most of other bands before. ...
9
votes
2answers
438 views

When writing about past research should I use the species name they employed or the modern version?

I am currently writing a literature review in which I am talking about the old research on the subject. When this research was carried out the species I'm talking about were classed under a different ...
2
votes
2answers
38 views

Are brain cells replaced over time?

You know how your cells die all the time and new ones are made to replace them, so you practically have a new body every maybe 5 years? Many people say you become a completely different person every ...
1
vote
1answer
28 views

Why are different lengths of nucleotides taken for structure prediction from an miRNA match area after BLAST analysis?

Generally in miRNA prediction most researchers do as Blast search with a set of miRNAs downloaded from miRBase with the parameters they require. Later on usually custom methods are utilized to get an ...
4
votes
1answer
47 views

How to search for organisms by criteria such as growth substrate and overflow metabolites?

I'm looking for a database or a systematic way to search for organisms which meet certain criteria. My criteria descending importance are: growth substrate chemolithoautotrophy (on which substrate) ...
3
votes
2answers
50 views

How to inactivate trypsin permanently by boiling?

I have to perform a hydrolysis of BSA with the enzyme trypsin. As a control I want to inactivate the trypsin enzyme. Can I inactivate it permanently by boiling (100oC) for 10 minutes, or does it it ...
4
votes
2answers
83 views

Does myopia tend to decrease the risk for later hyperopia?

It seems like being nearsighted for much of your life due to elongated eyes would make it easier in general to focus on near objects rather than far since the lens would not have to change much. Since ...
2
votes
2answers
37 views

regarding genetic disorders related to protein production

I am not completely familiar with biology, but i had a genetics course in college along with practicals. Forgive me if there is something wrong with my question. Is there a genetic disease which ...
1
vote
1answer
9 views

Can inverted microscope also be phase contrast microscope?

I've read somewhere that inverted microscope also allows phase contrast microscopy. Is that true? Do all inverted microscope have this ability? Do they have any other functions? Many thanks in ...
2
votes
1answer
71 views

How are arms different than legs?

Ok, this is a bit of a tangent question, but it came up yesterday and I didn't know the answer: How are arms and legs defined physiologically? For example, we say humans have two arms and two legs, ...
3
votes
2answers
70 views

What are the physiological roles of Hydrogen sulfide?

I am thinking why hydrogen sulfide has its effects in the body. For instance, it is one Salmonella's virulence factor. I am not sure if such a balance equations holds H2O + H2S ←→ ... Actually, I ...
2
votes
2answers
124 views

Can human survive falling from 30,000 ft without a chute?

If someone falls 30,000 feet without a parachute; what are the chances of survival if they landed in the water?
5
votes
2answers
6k views

Does sour food cause sweating?

While eating sour food or candy, I start to sweat if it's sour enough. My body feels much hotter although my actual temperature is the same, my forehead starts sweating a lot and I feel like it just ...
2
votes
0answers
26 views

How can eyeglasses improve vision beyond 100%?

The result of a vision test by my oculist yielded a result of more than 100% while wearing prescription glasses. I wonder, how can a person see better using eyeglasses?
0
votes
0answers
17 views

Why is drug clearance not affected by blood flow in case of low extraction ratios?

Background information: 1) Variation of organ blood flow: a) for drugs with low extraction ratio (<0.3): the venous drug concentration is virtually identical to the arterial concentration ...
1
vote
1answer
38 views

Can one use a hemocytometer on a compound upright microscope?

I'm posting this as a follow-up on What to look for when buying a light microscope?. The answerer states that you would need to use a an inverted microscope to count cell in the hemocytometer(counting ...
2
votes
1answer
33 views

How to prepare sample for SDS PAGE?

I usually take my protein sample 0.8ml and sample buffer(2X) 0.2ml for my sample preparation in SDS PAGE. Am I using correct proportion? my protein sample concentration is 4.1mg/ml. What is the ...
3
votes
1answer
28 views

How does the body really react to exposure to the vacuum of space?

I've seen sci-fi movies where the effects the body being unprotected in outer space are wildly different. In one movie, a guy radios for someone who's been ejected into space to breath all the air ...
4
votes
2answers
43 views

Do mosquito repellent sprays work on all insects?

I would like to know the mode of action of mosquito repellent aerosols. Are these specific to mosquito or are other insects affected too?
0
votes
1answer
36 views

What is this bizarre in-growth in this red bell pepper?

I just chanced upon a rather strange red bell pepper (or capsicum?): The green formation is bulbous to touch, just the like the outside of the bellpepper, and it has the same smell as one. I'm no ...
5
votes
1answer
27 views

What's the duration between the binding of adrenaline to β-AR and the first translated protein?

Binding of adrenaline (epinephrine) to the β-Adrenergic receptor leads to formation of cAMP (via G protein activation), activation of protein kinase A and subsequently to the expression of specific ...
1
vote
1answer
14 views

What is 'independent assortment'?

What is the definition of 'independent assortment'. I tried researching this term but came back with two results: alleles assort themselves independently of different alleles the alignment of ...
0
votes
0answers
13 views

When birds fly in V-formation, how is it decided which bird gets to be the 'lead bird'?

I've read on Wikipedia and in other sources that the lead position alternates due to flight fatigue, but does it alternate between a small cabal of of leadership birds, or does (almost) every bird get ...
0
votes
0answers
13 views

Congenital blindness due to retinitis pigmentosa - does it exist?

Retinitis pigmentosa (RP) patients typically become blind after a period of years in which their eye sight slowly deteriorates. Generally they develop night-blindness first, then tunnel vision, after ...
3
votes
1answer
44 views

Basic question about multiplex PCR

Let's say I have a DNA sequence with the following structure: $$ 5' - N_n - S_1 - N_{1000} - S_2 - N_{1000} - S_3 - N_n - 3' $$ Here, the $N$s represent stretches of arbitrary sequence of the ...
2
votes
2answers
128 views

Why would an Eadie-Hofstee Plot be non-linear?

Besides cooperativity between multiple active sites on an enzyme, what are the other reasons for the Eadie-Hofstee plot to be non-linear?
2
votes
2answers
50 views

How hot can a beverage get without burning the tongue?

What is the maximum temperature that a beverage can be without burning the tongue? I suspect there's a maximum temperature that the surface tissue of a tongue can withstand without being damaged ...
4
votes
0answers
23 views

Why does a pacemaker have a negative pulse?

Why is the first phase of the pulse from a pacemaker cathodic?
0
votes
2answers
29 views

Resource for finding what proteins bind?

I have two lists of proteins (including phosphorylated forms). I want to determine which members of each list act on which members of the other list. What online resources exist for finding out what a ...
2
votes
0answers
23 views

Which agricultural crops attract rodents?

Are there any data on rodent populations per acre of different types of agricultural crops?
6
votes
1answer
123 views

Find protein homologs with BLASTp

I'm trying to find homologs of a set of proteins using BLASTp. I'm working with custom databases. I'm using evalue of 0.00001 as threshold. I would like to filter queries having hits with >90% ...
0
votes
0answers
22 views

Is “Cisternal Twins” an actual classification of twin types and if so, describe it

I always assumed my brother and I were identical twins. Now my mother, in her eighties, says we are cisternal twins, but I cannot find any references on the matter. Is there such a thing? If so, what ...
13
votes
1answer
1k views

Why do we have five fingers?

Humans (and other humanoid mammals) have five fingers in each hand. Curious to know, why five? Wouldn't more fingers be more useful? Is there any evidence that it used to be different and natural ...
0
votes
0answers
12 views

Where can I find the details of kinematics data, physical parameters, and anatomical data of the forelimbs and hindlimb of a kind of horse?

Now, I am doing a simulation of horses, and some basic data, such as weight, height, species should be obtained to model a virtual horse. I am a student of engineering, and want to find some ...
0
votes
1answer
24 views

Apoptosis vs necroptosis

I understand that apoptosis and necroptosis share the same upper part of the pathway, but I cannot seem to distinguish when is each one activated? From my readings, it seems that when procaspases 8 or ...
0
votes
1answer
25 views

Clarification on BMI formula

In the BMI formula= (Weight) X 703) / (Height^2) , what does 703 represent? What is a reliable source that I can cite for this answer? weight= lbs height= inches
-3
votes
0answers
36 views

tBLASTn can't find some annotated proteins. Is it possible?

Seems tBLASTn can't find some of the proteins that were annotated using PGAAP pipeline. Is it possible tBLASTn can't find some proteins? Here is the code and the results: BLASTp proteins against ...
1
vote
1answer
70 views

Difference between thylakoids and lamellae in a chloroplast?

I'm slightly confused as to the difference between thylakoids and lamellae. My understanding was that thylakoids are 'discs' that are stacked into grana and there is a membrane between the grana ...
1
vote
2answers
53 views

Book Recommendations: GRE Subject Test In Biochemistry, Cell And Molecular Biology

There are probably a lot of really good answers that may vary significantly in terms of content. I'm looking for a set of books that I can read in preparation for the GRE Subject Test In ...
2
votes
1answer
30 views

What are the features on a microscope one needs in order to do lab work?

By lab work I mean urinalysis, blood work(live as well), fecals, cytologies, histologies and all other. I have read(partly) a book(from 2002) on lab diagnostics and the author did not mention ...
0
votes
1answer
16 views

What is the most common way of administering loop diuretics and what is their time course of action?

I am reviewing some material on loop diuretics, and I am curious to know how these drugs are administered. Also, I am interested in knowing their time course of action once they are administered into ...
0
votes
0answers
13 views

How do I label tendon cells?

How could I label tendon cells in drosophila and observe the cells using a fluorescent microscope? If possible please be specific in the answer.
2
votes
1answer
61 views

What are some of the general characteristics of the DH5 alpha strain?

I can not find some useful sources unfortunately. Please tell me about some important characteristics of DH5 alpha. What makes DH5 alpha suitable for the gene cloning?

15 30 50 per page