4
votes
2answers
186 views

What if a Point Mutation is seen in only half the coverage for its location?

I've been looking at some sequenced exomes and found an interesting point mutation that causes a Proline-to-Leucine amino acid change in the protein. This seems like it could have a big impact on the ...
2
votes
1answer
31 views

Why does the pET- expression vector contain a LacI gene additionally to the one in the genome?

The pET plasmid is used for protein expression with T7 promotor in expression strains, such as E.coli BL21(DE3) It contains a lacI gene which codes for the lac repressor protein, a protein of ...
2
votes
2answers
516 views

Animal altruism?

My first impression of http://www.youtube.com/watch?v=Za4uT1ob8qE is that the baboons were being altruistic and benevolent, but is this guess proven or affirmed by science? I sense there's more to ...
2
votes
2answers
38 views

Bohr effect in respiration

Would I be correct in saying that the Bohr effect is ONLY related to the concentration of [H+] in the tissues. Obviously the concentration of carbon-dioxide can contribute to this concentration by ...
6
votes
3answers
102 views

Possible reasons for DNA getting stuck in well

I am in the process of Library preparation for Illumina MI-Seq using mtDNA. Using NEB Hotstart LongAmp Polymerase, I was able to obtain upto 10kb amplicons of mtDNA. However, when I switched to a ...
1
vote
1answer
35 views

How to selectively download 3 fields from every record in UniProtKB?

I want to download a comprehensive table of protein names from uniprot.org. More precisely, I want to generate a tab-delimited table consisting of the id ...
7
votes
1answer
116 views

Why Did 6 Great Ape Species Survive But Only 1 Hominid Species?

My dad asked this question when I explained some circumstances surrounding Homo sapiens survival against all odds. We know there is only one hominid species left in the world, Homo sapiens. We also ...
10
votes
1answer
617 views

Can butterflies still fly after their wings are touched?

I have a question about butterflies: When I was a kid, I was told not to ever touch a butterflies wing, otherwise it would loose its ability to fly. This was connected to some dust which was supposed ...
3
votes
1answer
109 views

Is it true that viruses cannot be seen with phase-contrast microscopy?

There appears to be a lot of material on the internet claiming that viruses can be only seen with electron microscopes, and not with light microscopes. To the contrary, for example this old paper ...
7
votes
4answers
292 views

Extinction of species

I barely know anything about biology and realize that this might be a stupid question, but I'll ask anyway! I know that species "transform" into other species through the process of evolution. Many ...
6
votes
2answers
63 views

Which species were first described by Charles Darwin?

There are many plants and animals named for the naturalist Charles Darwin, such as Darwin's Frog (Rhinoderma darwinii), but which were named by him? I'm finding it difficult to find such a list.
7
votes
3answers
101 views

Where does extra blood come from to fill your muscles during exercise?

Let's say I go to the gym and lift some weights an hour. During this time my arms will grow due to the "pump" -- the extra blood rushing in to feed the muscles. For example, I've measured about 2-3 ...
0
votes
0answers
22 views

Are there any arboreal mammals which eat leeches?

Are there any tree-dwelling mammals which might be found taking a living leech and eating it? (And do any hollow-dwelling birds of prey eat leeches?)
4
votes
2answers
32 views

What is the biological principle of this “holotropic breathwork” technique?

Holotropic breathwork is a non-drug technique developed by Stanislav Grof used in psychotherapy. The therapy as a whole is usually called holotropic breathwork (at least by Grof himself) and will most ...
1
vote
1answer
22 views

Is this pCAMBIA co-transformation possible?

I would like to know whether the pCAMBIA1200 and pCAMBIA0380 be used for co-transformation of rice using Agrobacterium LBA4404.They probably have the same ori site so they belong to the same ...
1
vote
1answer
22 views

What is a “scutella of reindeer”?

In the film "The Perfect Human Diet" at 32:36, a scientist points to animal remains and states "we've got a scutella of reindeer there." What is scutella in this context?
2
votes
1answer
42 views

Leaves not liked by herbivores

I at times have many goats grazing in our locality. Recently a few cows were seen too. I just offered one of the cows a few leaves from Jasminum sambac plant growing in our garden. The cow smelled and ...
2
votes
3answers
60 views

Is there an evolutionary purpose to armpit perspiration?

Given that most Americans seem to do fine using anti-perspirant, it got me wondering: is there an evolutionary purpose to underarm perspiration? I can't imagine it acts to attract the opposite sex. ...
0
votes
0answers
36 views

Poly Unsaturated Fatty Acid

PUFAs spontaneously oxidize, speed up the process of glycation, since too much glucose and they also decrease mitochondrial respiration - more oxygen and CO2, less lactate and PUFA also promote ...
1
vote
1answer
23 views

What is the specific use of a capsule in E.coli?

In evolutionary terms, will the capsule cease to exist someday or will it improve ? Does it provide any boost to the organism in any way which harms us ? Can't we remove capsules or engineer E.coli ...
2
votes
1answer
58 views

Why do humans seem so much more prone to disease than animals? [closed]

It seems like when we observe animals in the wild, the occurrence of noticeable disease in adult individuals is much lower than in humans. Why? There are a number of reasons that this could happen, ...
3
votes
1answer
42 views

Cicada Killer Behavior

I've had a giant wasp hanging around outside my window the past few days, so I've resolved to learn more about it. I believe it is a male Cicada Killer, because it displays the following behavior: ...
3
votes
2answers
192 views

Why doesn't the human skin grow back exactly how it was before being damaged?

Very simple: Why (and how) is regenerated skin different from original ? As we know we lose skin cells that becomes the dust in out homes and it always grows back to full thickness right ? So when I ...
2
votes
0answers
14 views

How are HSPs identified in UCLUST

I am new in Bioinformatics and recently studying UCLUST algorithm by Robert Edgar(2010). It is a fast sequence searching and clustering algorithm. The algorithm is heuristic based. I studied that ...
3
votes
1answer
43 views

Why don't animals produce alcohol

As I understand during anaerobic respiration, the electrons received from the decomposition of glucose are transferred to nicotinamide adenine dinucleotide and flavin adenine dinucleotide. As there is ...
2
votes
1answer
38 views

naturally occuring fatty acids

The double bonds in naturally occurring fatty acids present in our body are in cis configuration while the enzymes for beta oxidation can act on trans double bond. Then what will be the significance ...
6
votes
4answers
152 views

Books for biology,popular but rigorous? [closed]

There are very goods in physics and mathematics (like Feynman lectures for exemple) that give an elementry but but broad view of their,therefore I'd like for help to find help to find a biology book ...
4
votes
1answer
49 views

How to measure trajectory regularity?

I have two animal running trajectories. A regular one with repeated back and forth running between point A and B, like the one on top in the figure. The other one is very irregular, animal paused and ...
2
votes
3answers
506 views

DNA is charged negative. Where is all the positive charge in my body?

DNA is charged negative because of its phosphate backbone. Since charges need to be balanced (so that there are no charges building up somewhere), what is the positive charge which neutralizes this ...
1
vote
0answers
30 views

Based on which criteria should i choose the control sample to calculate delta_delta_Ct

I want to calculate the gene expression of an experiment with the Delta delta Ct method. I have these results from two runs of a real time PCR (one for the GOI(Gene of Interest) and one for the ...
0
votes
2answers
62 views

Questions about the Evolution Theory [closed]

I'm trying to understand more about evolution theory. (I am coming from a mathematical background) What i understand till now is that the "improvement" of the dna is a purely random development and ...
6
votes
1answer
50 views

How do I read a sequence logo?

I'm looking at a paper that uses several sequence logos to illustrate the consensus sequence of certain sites. Here is the most important of the sequence logos I'm interested in: The explanations ...
2
votes
1answer
31 views

What makes certain obligate anaerobes viable in fermentation starter cultures?

If Propionibacterium are obligate anaerobes (to wit, poisoned by oxygen), what makes 'Dairy' or classical propionibacteria (e.g. P. shermanii, P. jensenii, P. acidicpropionici, et al) viable in ...
4
votes
1answer
56 views

Which turtle species was on board the Iranian Space Agency launch in 2010?

They are seen briefly in this video: https://www.youtube.com/watch?v=nZoNdf6hlII The launch made international news, but there was no mention of the species. Here's the entirety of Wikipedia's ...
4
votes
2answers
199 views

Why is assembling paired end illumina without any input parameters an important problem?

In one of the comments in this question about multiple sequence alignment, it was stated @5heikki: btw if you want a good bioinformatics problem, come up with an assembler that assembles any ...
-1
votes
1answer
56 views

What organism most efficiently converts a given quantity of mass into heat?

Question I am trying to answer: In the popular film The Matrix, heat given off by humans is harvested as an energy source. I wondered, if this were possible, would humans be a good organism to ...
8
votes
1answer
292 views

What kind of skull is this?

We found this skull on a beach in southwestern Sweden, and are curious as to what kind of animal it was.
2
votes
2answers
45 views

Conservation Genetics - Book recommendations

Can you please give me some advice for a book in (evolutionary) conservation genetics that offers an in-depth review of the mathematical formulations used in this field. I read the book Evolutionary ...
5
votes
1answer
60 views

No digested product bands, but markers are visible. What could be the reasons?

I had to digest my insert containing plasmid and another vector into which my insert has to be ligated, each with BamHI and NotI. Upon digestion, I ran the gel to check for results. The digestion ...
6
votes
3answers
329 views

What exactly does adaptive mean?

This is a quote from Dey et al 2014: Hatching asynchrony is thought to be adaptive because... What exactly does adaptive mean here? Does it mean hatching asynchrony has fitness benefits? Or does ...
1
vote
1answer
50 views

Is the human collarbone supposed to break?

I've read it in a book and have heard people mention it, but I can't find any documentation about it. But the point being made is this: The human collarbone acts as a shock absorber for impact on ...
2
votes
1answer
44 views

Standard datasets for testing new multiple sequence alignment algorithms?

Are there any open and freely distributed standard datasets for testing new algorithms for multiple sequence alignment?
3
votes
1answer
83 views

What is the state-of-the-art algorithm for multiple sequence alignment?

Which algorithm or algorithms are considered the standard or state-of-the-art for multiple sequence alignment? How big is the need for better algorithms? How many sequences need to be alignment in a ...
3
votes
1answer
54 views

Mammal body density

Human body density is approximately 1 kg/l (same as fresh water) - which is not surprising (at first) given that we are 70% water, and is surprising (on a second thought), given that the other 30% can ...
2
votes
0answers
21 views

What does salt do to styes?

Styes formed in the eyes are cured by dabbing at them with lukewarm water(I saw this being done many a times as a home remedy.) At times, common salt is added to the water before dabbing. My mother ...
3
votes
1answer
23 views

Is there a realistic “family tree” model for the human population?

I would like to do some gene inheritance simulations, and I was wondering if there is an accepted model for what mating looks like in the general human population? I am not necessarily looking for the ...
3
votes
1answer
45 views

Is there revolution in humans since the first homo sapiens sapiens

In a thinking mood, I was wondering if the human species has changed since they have first become homo sapiens sapiens. I asked myself this question because I was thinking about how we will evolve ...
1
vote
2answers
40 views

Recommended sequence clustering algorithm for transcriptome data

I'm working on a project where I'm going to analyze a large amount of transcriptome data. After assembling our RNA-Seq reads into contigs using Trinity, it looks like I'm going to have about 10GB of ...
3
votes
1answer
70 views

How to remove bad lanes in ImageJ Westernblot analysis

I use ImageJ to do an analysis of a Westernblot Image. If everything goes as wanted the workflow is fine. But if I do something wrong creating a lane there is no undo for a lane and also no way to ...
1
vote
0answers
44 views

Can two women have a daughter? [duplicate]

In vitro fertilization is a pretty routine procedure in which a female egg is fertilized by sperm outside the body. Recently apparently there has also been something similar in which the genetic ...

15 30 50 per page