1
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0answers
85 views

Which mutagen is responsible for pancreatic cancer [closed]

Which mutagen, aflatoxin or acridine orange (an intercalating dye) would most likely lead to an increased incidence of pancreatic cancer. Why?
1
vote
1answer
61 views

Is there any documented case in which floating substances led to the introduction of a new species?

Floating substances in the marine or freshwater environment often carry hitch hiking organisms. It is argued that many species may use this dispersal vector to reach new habitats, however to my ...
1
vote
2answers
31 views

Tapeworms and their effect on humans

I've read that some people in some countries actually use tapeworms as a form of losing weight. What are the dangers to these people?
0
votes
0answers
22 views

Why not use CIE color scheme for descriptions of mold colonies?

Reading a mycology paper recently I noticed that the authors refer to Ridgeway nomenclature, which as I understand it entails 1115 colors with distinct names like 'Artemisia.' My question is, ...
1
vote
0answers
18 views

condensed protocol for sequencing a portion of human DNA from buccal sample

Anyone have a short & sweet protocol for PCR amplifying a region of human DNA (chromosomal or mt, I don't care) extracted from a buccal sample: including validated primer sequences and preferred ...
1
vote
1answer
45 views

Do you have experience with PacBio?

I prepare a experiment and I found $PacBio SMRT$ as the great way to sequence my PCR products. I find the cost: library preparation 655 dollars + sequencing 435 dollars. It seems very low. Do you have ...
2
votes
0answers
37 views

Book recommendation on selective breeding?

I am looking for a good book that gives a nice overview of the science and technics of selective breeding. I am particularly interested in the use of population genetics to support decision in this ...
2
votes
3answers
97 views

What exactly is Tinnitus?

What exactly is tinnitus? What is it's cause, and why do some people hear it and others don't?
1
vote
0answers
17 views

Triune brain model

I have heard about the triune brain model in biology class, but my teacher does not know much about it. What I'm curious about, is this model accepted by most of the neuroscientists, or is it just a ...
3
votes
1answer
35 views

Literature about industrial enzyme application

Can someone point me out to literature (reviews or better workbooks) describing industrial chemical reactions catalysed by enzymes? What I'm most interested in are case studies of how the output of a ...
2
votes
2answers
41 views

Can excessive carbonated drink consumption lead to elevated red blood cell levels?

I've recently had a blood test and the results displayed elevated levels of erythrocytes as well as hemoglobin. (As a result my hematocrit levels were also above average) At my workplace there is a ...
0
votes
0answers
6 views

What is CT of tertiary villus in placental barrier?

Context Placental barrier consists of CT of the tertiary villi. It something Chorionic ... I think it cannot be cytotrophoblast because it does not have tertiary villus. If it was, it would ...
0
votes
0answers
6 views

Does maternal part of placenta have tertiary villus?

I am thinking between these two definitions Maternal placenta = decidua basalis + placental septas and Maternal placenta = decidua basalis + tertiary villus I think placental septas do ...
2
votes
1answer
47 views

What is the energy consumption of the brain?

What is the energy consumption of the brain, and is there a difference in consumption when waking and sleeping?
0
votes
0answers
17 views

To show smooth chorion and villous chorion from these pictures

Can you see smooth chorion and/or villous chorion in the pictures below? I am trying to visualise them. Some definitions: Smooth chorion = Chorion leave. Villous chorion = Chorion frondosum. ...
1
vote
1answer
46 views

Population Genetics Question

Can someone please help with this question? Here is my working (just in case it is not clear: 1/300*1/30*1/2) but is this actually correct or do I need to multiply by 0.5 once again? I appreciate ...
2
votes
1answer
26 views

How does DNA resolve on size exclusion resin?

We generally have a good idea of how DNA separates using agarose gel electrophoresis, how well does DNA resolve on a SEC resin like superose? I get the impression that salt influences where it elutes. ...
1
vote
0answers
24 views

What is TILLING?

How does TILLING (Targeting Induced Local Lesions in Genomes) work? What is needed to perform a TILLING experiment, and what kind of information can we get out of it?
0
votes
0answers
15 views

Normal Frequencies in Heart

I am trying to visualise possible ranges of frequencies from different structures of heart: different valves and their possible frequencies heart sounds I - IV and their normal frequencties what can ...
0
votes
0answers
10 views

How do the symptoms of Sepsis affect heart function?

I have intuition that Sepsis with infection can cause spastic functioning of heart during systole heart working spontaneously in snatches during systole atrial fibrillation first contraction of ...
1
vote
1answer
73 views

What does it mean for a chemical pathway to be conserved?

In many papers the MAPK pathway, (along with many others) is referred to as being conserved: Example: "The mitogen-activated protein kinase (MAPK) cascades are ubiquitous in eukaryotic signal ...
2
votes
1answer
50 views

How to measure quality and quantity of DNA?

I would like to mesure DNA. I quantify the concentration with Qubit fluorometer, but I would like to know also quality of DNA. I try BioAnalyzer (Agilent),but without success. Bioanalyzer measure DNA ...
2
votes
1answer
67 views

dopamine paradox in Schizophrenia

If there is more dopamine action in the mesocortical pathway in Schizophrenia, then the patients should always be in euphoric state. But instead patient lacks motivation and don't want pleasure. Why?
3
votes
1answer
56 views

How do I get a brighter DNA bands

I've been trying to a good contrast for my DNA bands, however I haven't been so successful. I've been running the ladders only to make sure I get a good contrast before further experimentation. I ...
1
vote
1answer
20 views

How can a YAC be screened against a cDNA library and a cosmid library of genomic DNA to find a gene?

I am reading fw2.2: A Quantitative Trait Locus Key to the Evolution of Tomato Fruit Size (doi: 10.1126/science.289.5476.85). The authors are trying to find the gene that makes tomatoes bigger. As far ...
2
votes
0answers
12 views

Cosmid genomic library, or genomic cosmid library?

Which usage is better for referring to a "cosmid library of genomic DNA fragments"?
0
votes
1answer
23 views

How does mutation work natural population? and is it common in protists or prokaryotes?

Why is it inevitable that evolution by mutations alone should be a common cause of evolutionary change in most natural populations? And do you expect mutation-driven evolution to be more common in ...
5
votes
1answer
39 views

Is a sequential double transformation acceptable?

Standard protocol states having two compatible vectors being transformed simultaneously during the same procedure. I've come across a situation in which transforming one vector, obtaining results, and ...
2
votes
0answers
17 views

What are the roles of guanidine-HCl and ethanol in binding of DNA to silica?

I'm trying to understand how exactly the binding to silica gel (in kits) step works and I cannot find any papers which provide an explanation of the physics or chemistry; especially on the way that ...
8
votes
3answers
799 views

Was the mitochondrion or chloroplast first?

I still don't know if the mitochondrion or chloroplast was first? I've looked for it on the internet and in various books but haven't found anything. Does anyone have the answer and a theory which ...
1
vote
1answer
40 views

Life on other planets [duplicate]

first of all I'm not sure if I should post this here or in the astronomy stack. I think biologist are more likely to answer my question. To make it short: Why when looking for any sort of life on ...
1
vote
1answer
58 views

What does min mean?

I read that "trp operon is located at 27 min on E.coli chromosome." What does "min" mean ?
0
votes
1answer
26 views

How do I determine the parental type here?

Please consider the following question: You cross Drosophilia and receive the following offspring: 965 wild-type, 944 black- vestigial, 206 gray- vestigial, and 185 black- normal. Explain your ...
0
votes
0answers
9 views

What causes human hair to go gray? [duplicate]

What causes the graying of human hair? Additionally, why does extreme stress bring it on faster?
1
vote
1answer
37 views

How do Gram + bacteria use a proton gradient for F-type ATPase?

Does anyone know of any papers showing how Gram positive Fermiculates or Actinobacteria use a H+ gradient for an F-type ATPase, It seems impossible since there is no outer LPS membrane to maintain ...
0
votes
1answer
22 views

Property of E.coli harboring F element

From Genetics by Ursula Goodenough E.coli harboring an F element are endowed with a number of phenotypic traits : 1.They are sensitive to infection by ssRNA phages and certain ssDNA phages. ...
2
votes
2answers
69 views

Effect on fitness of mutations

What is the distribution/probability density function (PDF) of impacts on fitness of new mutations? I very welcome any partial answer that does not give the whole PDF but just some information ...
2
votes
1answer
30 views

Dominance/recessivity of new mutations

What is the distribution/probability density function (PDF) of recessivity/dominance of new mutations? I very welcome any partial answer that does not give the whole PDF but just some information ...
2
votes
1answer
45 views

Additive genetic variance with $n$ loci

The genetic variance of a quantitative trait (the quantitative trait in question is fitness) can be express as the sum of two components, the dominance and additive variance: $$\sigma_D^2 + ...
1
vote
1answer
72 views

Additive genetic variance with $n$ alleles

The genetic variance of a quantitative trait (the quantitative trait in question is fitness) can be express as the sum of two components, the dominance and additive variance: $$\sigma_D^2 + ...
3
votes
1answer
34 views

Brainbow fluorescence labeling technique

I have this picture describing the Brainbow system here: The paper I am reading says it uses Cre-lox recombination to express a certain color. If the cell in question was expressing a yellow ...
1
vote
2answers
48 views

In what ways can mechanisms of apoptosis be damaged?

How many ways can an Apoptosis mechanism be made disfunctional or irreparably damaged? If a cell has damaged Apoptosis mechanisms and it divides will its daughter cells have such damage?
0
votes
0answers
19 views

People say the cardiac cycle depends on blood pressure? How so?

I know what the cardiac cycle is. There are two phases diastole and systole. One is the relaxed, and the other is the contracted form of the heart. The diastole is where blood is pumped to the ...
2
votes
2answers
33 views

Role of Melatonin in Circadian Rhythm

Melatonin regulates the wake-sleep cycle by causing drowsiness and lowering body temperature, but which specific chemical pathways lead to this drowsiness?
1
vote
3answers
49 views

Does DNA influence death (e.g. by cell aging)

I know that we age because of errors in the cell replication process and these errors eventually leads to death. If we exclude environmental factors, are there any other factors that can cause these ...
2
votes
2answers
47 views

Real time PCR normalization algorithm

When performing normalization of real time PCR results, I found two ways of doing it: In my lab they follow the next layout: $efficiency^{-(CT\ interest\ gene - CT\ housekeeping)}$ each time ...
1
vote
1answer
27 views

Nervous System : Organ or Gland Failure

If an organ was to start failing, not total failure, such as the thyroid gland, is it possible that the fault may not lie in the failure of the organ but in the nerve signals coming from the brain?
1
vote
1answer
23 views

Nervous System : Signal Translation

If a device were created to monitor the nervous signals from the heart, including stable heartbeats, rapid heartbeats, etc., and this device were capable of transmitting the "stable heartbeat" to the ...
1
vote
1answer
21 views

Nervous system : Nerve signals [duplicate]

If the electrical signals from all the various organs throughout the body eventually connect to the nerves in the spinal column traveling up to the brain, how does the brain differentiate the ...
1
vote
1answer
28 views

Nervous system: Pain and Pleasure?

If you have a constant pain in your body, why does a greater pain or pleasure make you not feel the original pain?

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