-1
votes
1answer
32 views

What is phenotyping of blood during transfusion process?

Thallaesemic patients are advised to have only "phenotypically matched blood".I mean the transfusion problems arise mainly due to "grouping problems" like mismatch b/w ABO & Rh+/- so what do this ...
1
vote
0answers
13 views

What is the best way to perform ultra-deep targeted sequencing of few samples?

I performed recent whole genome/exome sequencing in tumor samples and now I would like to take a few of these variants (say maximum 50 amplicons) to: 1) Validate them in the original samples 2) ...
2
votes
1answer
28 views

Does a bird know it's different? [closed]

I live in Northern Virginia, USA. As I am sitting here there is a large flock of small (blackish) birds in a tree. They are quite prevalent here. For the sake of brevity I will call these birds ...
-2
votes
1answer
53 views

Can a rotten egg actually hatches to a real life?

First of all, I'm no expert in biology. I failed a lot in high school. Please bear with it if it's a stupid question. For the past few days, I got a rotten smell as if an animal died in my kitchen. I ...
5
votes
1answer
249 views

Do the right-handed people tend to use the right side teeth of their jaw to chew food more often than the left-handed people?

And vice versa, do the left-handed people tend to use the left side teeth of their jaw to chew food more often than the right-handed people?Or the frequency of food chewing distribute fairly to both ...
0
votes
1answer
19 views

Which three functional groups are found in the general structure of amino acids? [closed]

Yes, I know, not two, but THREE. EDIT: Yes, sorry but I did quite a bit of research, searching my notes and textbook for the answer, but all I could find was TWO functional groups as a opposed to the ...
6
votes
1answer
72 views

Can rapid eye blinking induce epileptic seizures?

Photosensitive epilepsies are characterized by visually-induced seizures. Flashing-light stimuli are known to induce seizures in some (but not all) patients. My question is whether people with this ...
2
votes
1answer
227 views

Is sexual selection evolutionarily advantageous?

Our biology teacher says that peacocks will soon become extinct because of bigger and bigger (and harder and harder) tails. Why natural selection did not eliminate sexual selection?
1
vote
0answers
13 views

how to get truseq's gel size selection when preparing libraries for exome enrichment?

i would normally use a 6 minute fragmentation as quoted by agilent but this gives post exome enrichment libraries of around 300bp or slightly over. Truseq want libraries to be 100bp larger. i am ...
1
vote
1answer
29 views

PCR directly on DNA binds to Ampure XP

During the library preparation it's possible to conserved Ampure beads during the different enzymatic reaction (it's the with beads strategy for improving yield, Fisher et al. Genome Biology 2011). ...
1
vote
1answer
13 views

how to re-sequence a few Mb of genomic sequences?

If I want to re-sequence a known part of the chromosome (we know the reference genome sequence) of ~1Mb to find the possible mutations (SNP or INDEL) that caused a certain phenotype. What is the best ...
2
votes
1answer
24 views

Separation of closely-sized isoforms

I have to separate two proteins of 86kDa and 80kDa respectively, however, I just cannot get a decent separation even in 6% polyacrylamide gel. To make matters worse, these two proteins are isoforms ...
0
votes
1answer
13 views

Rosetta strain with chaperones for protein expression?

I am trying to purify a protein, and I was wondering if it is reasonably straightforward to obtain E.coli cells containing: -pGroe plasmids expressing chaperones. -Rosetta plasmids with codons that ...
-2
votes
1answer
34 views

How does a multicellular, eukaryotic body treat heat-resistant pathogenic bacteria?

How does the said body treat heat-resistant pathogenic bacteria? If fever is the body's response to foreign microbial invasion, then what happens if the bacteria is heat-resistant but the body's own ...
0
votes
0answers
19 views

General time reversible model of evolution and Felsenstein model

I would like to get suggestions for the books related to General time reversible model of evolution and Felsenstein model etc. Specifically the mathematical treatment of these topics and concepts ...
0
votes
1answer
21 views

Intro Bio question: dihybrid punnet square

Today we had our exam. There was a punnet square for two genes. and when I multiplied , for example, Ry × rY I wrote the answer as ...
0
votes
0answers
36 views

From a list of protein accessions, how can I retrieve DNA sequences from GenBank?

I have 1000's of protein sequences in FASTA/their accession numbers. I want to go back into the whole genome shotgun database and retrieve all DNA sequences that encode for a protein greater than or ...
4
votes
2answers
69 views

How does the eye know whether to focus further out or nearer in order to bring a blurry object into focus?

If the eye sees an object, but the object is out of focus, the eye could be focusing too near or too far. How does the eye (or the brain) distinguish one situation from the other? How does it know ...
5
votes
3answers
107 views

What makes DNA sticky-ends sticky?

When restriction enzymes jaggedly cut double stranded DNA it results in so called sticky ends. What is the substance that makes the DNA sticky?
1
vote
0answers
34 views

Modified Mendelian Ratios Problem

I'm studying modified Mendelian ratios. I've got a theoretical problem that I'm stumped on: A species of bird may have normal wings, or a mutation that causes frazzled wings. I do 15 identical ...
2
votes
1answer
50 views

Why do we have two of some organs, but not all? [closed]

We have two eyes, but we don't have two hearts. Why do humans have two of some organs, but not all?
4
votes
1answer
31 views

A few questions regarding immunology [closed]

I know that there is a variable region on antibodies which can recognize a wide variety of antigens, and that germinal centers create more "fit" antibodies to respond to an infection. So I was just ...
7
votes
3answers
68 views

If my brain lost all of its activity for a moment, would my memory be gone too?

I can't remember which question it was, but in the past here, it was explained to me that memory is actively stored in the brain, rather than "physically". Meaning, memories are made up of the brain's ...
1
vote
1answer
61 views

Why can't people talk while inhaling?

Why do we have to exhale in order to talk? From looking on Wikipedia, it seems like it has something to do with the glottis, but I'm not clear on the mechanism that makes speech sound so different ...
1
vote
1answer
31 views

Anesthetics, specifically inhaled anesthetics

I have had a look at previous inhaled anesthetics and many of them appear to be fluorocarbons. What could be the mechanism behind fluorine's anesthetic properties? Is it the specific bonding pattern ...
2
votes
1answer
48 views

Duration of selection and survival of the fittest

I feel there is a part of the theory of evolution I'm not understanding correctly or maybe I'm missing it, but would like cleared up. From what I understand, over time random mutations occur which in ...
9
votes
3answers
153 views

What causes feeling of feet falling asleep to be delayed?

This answer explains the sensation of extremities "falling asleep" as a result of sustained pressure on a nerve. The answer links to an external web page that says that the feeling "quickly goes away ...
4
votes
0answers
45 views

Why do some internal organs regenerate?

I have been reading about the human liver and zebra fish heart muscle having the ability to regenerate. It seems to me that these organs have very little chance to become damaged or worn out. At the ...
0
votes
1answer
20 views

If gene differs between chromosome pair does individual get a mixture of protein shapes?

Do diploids such as humans end up with a mixture of two different shaped proteins where the appropriate gene is expressed differently in the two halves of a chromosome pair or is there some governing ...
3
votes
1answer
33 views

What are limitations on the number of objects the eye determine at a glance?

Take two people Sam (average human) and Jack. Jack says to Sam "I'm going to show you some skittles (or other object) and I want you to tell me as quick as you can how many I'm holding." He opens ...
1
vote
1answer
62 views

(how) is it possible to choose the biological sex of a child? [closed]

I recently read of a study that investigated the ethics of child's biological sex choice in humans, and now I want to find out how this can/could be done. Does anyone know of scientific papers or ...
3
votes
1answer
157 views

How is satiety measured?

I am aware of satiety only through experience, rules-of-thumb and possibly myths. For example: white bread is not filling, a pasta meal can be filling but because we "burn" carbs quickly pasta itself ...
0
votes
0answers
34 views

Are ipratropium and tolterodine parasympatholytic?

They are nonselective cholinoblockers and antimuscarinic. Other cholinoblockers of parasympaticus, which I know, are parasympatholytic such as atropine, butylscopolamine, trihexyphenidyl, titropium ...
1
vote
1answer
38 views

Low copy numbers of plasmids

I have a plasmid with the P15A origin which apparently has a low copy number (see here). This would explain why my purification yeilds for subsequent digestion are low (gel shows the plasmid after ...
0
votes
1answer
13 views

Differentiation of norepinephrine and epinephrine in indications

Norepinephrine is less beta2 adrenomimetic than epinephrine so more selective so less bronchospasm so may be therofore better in treatment of cardiac failure and different shocks. However, I am not ...
0
votes
0answers
24 views

How do you accurately measure anguiliform (eel) locomotion?

In one of my courses, we were discussing a paper by Muller and van Leeuwen (2006) which made a lot of comparisons the differences between carangiform (mullet) and anguilliform (eel) locomotion. While ...
0
votes
1answer
23 views

Is there a stem cell dye for education purposes?

I need a dye that will stain stem cells (for this purpose the stem cells will proquired from chicken bone marrow). This will be an educational hands on activity for young teenagers so ideally the dye ...
1
vote
3answers
116 views

Is there an online gene library? [closed]

Is there an online library that includes all known genes in stored in their nucleotide form of all living organisms? Note I am not necessarily looking for a human only database. If such a database ...
0
votes
2answers
56 views

Purifying a linear plasmid after restriction digest?

I expressed a yeast vector in E.coli and purified about 13µg of it. I then linearized it using a restriction enzyme, and attempted to gel purify it. I attempted this twice. The gel showed a clear ...
0
votes
1answer
17 views

How can Pyridostigmine have the indication of Myasthenia?

I am thinking how pyridostigmine can be used in the treatment of myasthenia gravis. Its similar compound (neostigmine) is also anticholine esterase. This compound has the indication of myasthenia ...
4
votes
2answers
43 views

Can the human eye distinguish colors in the periphery?

In the back of my mind I have the idea that human eyes can't notice the color of objects in the far periphery, and that any subjective perception of colors is done by the brain that tries to fill in ...
1
vote
2answers
115 views

Theranos blood test's Specificity and Accuracy?

I am studying the limitations of Theranos device. They have 238 documents in Google Patents Search (Inassignee as Theranos), here. Which patents are relevant for their device? They have 70 tests ...
1
vote
1answer
12 views

allogeneic organ transplant and the immune response

What happens when an allogeneic organ transplant is performed on an immunocompetent recipient vs. when it is performed on an immunosuppresed recipient using a donated organ with immunocompetent cells? ...
1
vote
0answers
9 views

What mechanism prevent Colchicum Autumnale from being affected by Colchicine

Colchicine is a chemical usually used to cause polyploidy mutant by inhibiting chromosome segregation. It is synthesized in the Colchicum Autumnale. But what mechanism prevent C. Autumnale from being ...
0
votes
1answer
33 views

How does anger relate to blood pressure?

Anger is an emotion generated by neural processes in the brain and is associated with elevated blood pressure. How can an emotion, which is totally related to brain, result in blood pressure changes?
12
votes
2answers
2k views

How was birth control realized in ancient days? [closed]

In ancient days, there were no birth control methods like condoms or contraceptive pills available. How did they control pregnancy?
3
votes
1answer
77 views

How to inhibit formation of specific antibodies (to antisera)?

Is there a way to inhibit an antibody response to a specific antigen using immunosupression? I am interested in reducing the anti-antibody formation to animal antibodies such as murine antibodies in ...
6
votes
1answer
75 views

How big should the human population be, as predicted by body mass?

I would guess there is a theory in biology which states that the population size for a given species is inversely proportional to the body mass of individuals in that species. In other words, there ...
9
votes
1answer
67 views

Is our color vision calibrated to sky, vegetation, and blood?

Our color vision is based on three types of receptors (cones) which are sensitive to three distinct locations on the spectrum: 420–440 nm, 534–555 nm, and 564–580 nm. We label them "red", "green", ...
1
vote
2answers
53 views

human anti-mouse antibody

I have heard about human anti-mouse antibodies (HAMAs) and read that HAMAs neutralize murine antibodies, therefore decreasing the effectiveness of those murine antibodies. Is this true that HAMAs ...

15 30 50 per page