0
votes
0answers
16 views

How can site-directed mutagenesis be used to suppress the production of anti-antibodies?

In a previous post of mine, I asked how to supress the creation of anti-antibodies in vivo. In the answer, it was mentioned that site-directed mutagenesis could be used. Currently, I can't find mcuh ...
3
votes
0answers
23 views

Well to well variation in thermal cycler fluorescence?

We have an old BioRad ICycler Thermal Cycler with MyIQ single color fluorescent detector. While it's meant for RT-PCR, we've been using it for melt curves and binding assays for different types of RNA ...
-1
votes
0answers
29 views

site directed mutagenesis [closed]

I have read about site directed mutagenesis (a method used to edit genes and DNA) and had some questions about it. How is site-directed mutagenesis done? I know they are a couple methods so what are ...
6
votes
1answer
54 views

Number of transcription factor genes in the human genome

What is the number of the transcription factor genes present in the human genome? Does this value differ compared to Mus musculus, Drosophila melanogaster, Arabidopsis thaliana, C. elegans and S. ...
3
votes
1answer
59 views

How to attract spiders to my house?

I've been living in my apartment for a year now, and noticed something lately. During spring and summer I have quite a lot of midges indoors, and even now there are some. But there are no spiders! So ...
2
votes
0answers
13 views

Resource for finding Repressive/Inhibitory factors for a given gene?

I have a list of genes for each of which I'd like to find: A list of transcription factors that up-regulate the gene A list of inhibitory factors that down-regulate it. I used this tool on ...
-1
votes
1answer
38 views

Effect of Western chicken and GH [closed]

I heard this clause from my teacher one week ago: Chicken are grown in half a year until eaten in Baltic countries - Estonia, Latvia and Lithuania, for instance, [probably in other parts of ...
1
vote
0answers
26 views

How does a bacterial colony, grown in a nutrient medium, change the chemical composition of that medium over time? [closed]

In order to culture cells, the nutrient medium they are grown in must contain certain chemicals and trace elements (e.g. Hams medium, for culturing human cells, and a less demanding solution for ...
0
votes
1answer
54 views

Can swallowed fingermails, hair, or skin get caught in your appendix? [closed]

There is a common saying in my place: If you eat your skin, hair or nails, it will be deposited in the cecal (Vermiform) appendix, and can cause appendicitis. (This is mostly told to children to ...
5
votes
2answers
84 views

Why deoxyribose for DNA and ribose for RNA?

Why is DNA made out of deoxyribose and RNA made of ribose? Why can't they both use ribose or deoxyribose? I think that the deoxyribose gives an advantage in storing genes, the job of DNA and ribose is ...
9
votes
1answer
112 views

Why don't McDonald's fries decompose?

So I was cleaning out my car and found a McDonalds French fry. as I don't eat anything in my car I know exactly who and when this fry is a result of. The when is + 10 months old and it could pass off ...
5
votes
1answer
62 views

Why does DNA have its name?

Why is DNA called deoxyribonucleic acid and not something else? I get the nucleic acid part (because that's what DNA is made of) but what about the deoxyribo- part, especially the ribo- part. Maybe ...
0
votes
0answers
15 views

Would biotechnology in agriculture actually decrease biodiversity, as many people say? Does it have to?

I've heard the claim that because farmers would be using the same seed, you would end up with an entire crop population that could be susceptible to a disease. Like if you had a diverse population of ...
2
votes
3answers
95 views

fastq-dump using mkfifo to stream data

So, fastq-dump has the ability to be run on just an SRA file accession number, such that the SRA is converted to FASTQ on-the-fly, and the SRA doesn't have to be written to disk. I'm curious whether ...
2
votes
1answer
29 views

Western blot alternatives

Given an in vivo sample from an experimental infection, I would like to see if a bacterial protein is present. I've been thinking about Western Blot using sera from animals infected with the ...
1
vote
0answers
12 views

Public Pacemaker database online?

I am looking for any database about pacemakers with the leads atria A right ventricle VR left ventricle VL I did not find any in Physionet. Is there any anonymous data about different ...
4
votes
3answers
49 views

Can an influenza virus carry other infectors with it?

This idea came to my mind when thinking about how many people become ill in many locations with similar symptoms (fever and rough cough) from Influenza. There are of course different types of ...
6
votes
3answers
518 views

Should I dilute DNA with water or elution buffer?

I've extracted DNA using a kit and final step is eluted with buffer. If I must dilute my DNA samples, do I use the same elution buffer or can I use milliq water?
1
vote
1answer
46 views

How many possible DNA strands in a single chromatin? [closed]

If a chromosome is replicating but not yet finished is it considered to be a single chromatin with two DNA strands? My question stems from this general biology testing question: Suppose a biologist ...
0
votes
0answers
27 views

Cigarette consumption dose-response function WRT health outcomes

I'm curious how health risks (mortality, lifetime probability of cancer, etc) change with cigarette consumption. Specifically, treatring cigarette consumption like a continuous variable, rather than ...
1
vote
0answers
10 views

How does air-liquid interface (ALI) culture work?

For studying diseases, such as chronic obstructive pulmonary disease (COPD), cells are often grown in air liquid interface. I understand that the common way of establishing this these days is to grow ...
0
votes
0answers
15 views

Terrestrial Vertebrate Limbs

This question is regarding the conversation that took place here: Why don't mammals have more than 4 limbs?. I'm not sure if someone mentioned this and I just missed it, but everyone is talking ...
1
vote
0answers
43 views

What is the origin of DNA? [closed]

Deoxyribose nucleic acid underpins most life on this earth. It seems to me that the structure of DNA is already reasonably complicated and a certain degree of evolution may have already occured rather ...
3
votes
2answers
113 views

Why do we have to exhale (or inhale) in order to speak?

Every time we speak, sing, or make any other kind of advanced noise with our throats, we exhale, or to put it that way, blow air through our throats. Why is this required? After all, speakers do not ...
1
vote
1answer
24 views

Are more animal species edible than plants?

While grocery shopping I was thinking that we restrict ourselves in regard to which animals we eat. I surely don't want to eat spiders and ants, for example, but I know that this is just because of my ...
1
vote
0answers
18 views

What do you NEED for a 100 meter sprint? [closed]

I was discussing with my friends the 100 meter sprint. Somehow, the topic changed to decreasing the time that the sprint would require: What parts of the human body could you feasibly remove to set a ...
4
votes
1answer
60 views

Why estimate linear and full (linear, quadratic, and correlational) selection coefficients separately?

"We then fitted a linear regression including all three life-history traits to estimate the vector of linear selection gradients, β, for each sex (Lande and Arnold 1983). A quadratic regression ...
0
votes
0answers
11 views

What's the more abundant biopolymer: chitin or (ligno)cellulose?

Chitin is considered the most abundant polymer after cellulose (e.g., Rinaudo, 2006). Does anyone know of specific biomass estimates in literature, probably also differentiating between terrestrial ...
2
votes
2answers
40 views

Hydrophobia Outside of Rabies?

RELATED: Why does rabies cause hydrophobia? Agony, Hydrophobia and viruses in the light of evolutionary principles Has hydrophobia been found outside of rabies? I have only seen it ...
0
votes
1answer
25 views

Deeper Learning in Physiology? [closed]

I am a Pharmacology and Toxicology major at a large state school. I was only required to take physics I and II. (algebra based) At first I thought that was fine, but I soon began to realize that I ...
1
vote
1answer
29 views

Is this a carpenter ant?

I stumbled upon a few hundred of these swarming around two different places in my living room today. They kind of look like a carpenter ant to me, but I'm not sure. Also, I'd love to know how you ...
2
votes
3answers
34 views

Long term storage of agarose-ethidium bromide gels that have already been cast

I have come up with what I thought was a clever idea: Store the agarose gels I pour, and only cut as many lanes as I need to run later, minimizing wasted agarose (and wasted effort/time) when I need ...
4
votes
0answers
57 views

Long-term effects of antihistamines on mind and CNS [closed]

Can maybe someone share knowledge or guesses on the following questions: Does the prolonged use of antihistamines cause long-term effects on mind or CNS? Are there any known evidences of their ...
1
vote
1answer
47 views

Does physical exercise following lack of sleep increase the risk of sudden cardiac death (SCD)?

Is there any scientific evidence supporting the hypothesis that physical exercise following lack of sleep increases the risk of sudden cardiac death (SCD)? The only relevant research I could find is: ...
1
vote
1answer
20 views

Why are nausea and dizziness such common side effects from medication?

Why are nausea and dizziness such common side effects from medication? If you go through your medicine cabinet and look at side effects, those might just be on every single bottle. Is there some ...
4
votes
1answer
67 views

How was the resemblance between apes and humans explained in pre-Darwinian biology?

Humans and apes have somewhat obvious similarities, these must have been apparent to natural philosophers before the possibility of a common ancestry was first proposed in the mid-1800's. These ...
3
votes
1answer
136 views

How long does it take for a cricket to die at subzero temperatures?

I am on a number of reptile groups and I often hear freezing recommended as a humane way to get rid of surplus feeder insects. However, they can also revive if you don't leave them in there long ...
2
votes
1answer
38 views

What percent of the population has some form of synesthesia?

Data I am trying to get my hands on: What types of synesthesia (e.g. Grapheme-color synesthesia, Chromesthesia, etc.) are most common across the population? What percent of population has that ...
0
votes
1answer
27 views

PCR cycle problem [closed]

IF I began a PCR cycle with 5 copies of a particular DNA section, and copied the section by PCR, for 6 cycles, how many copies of the DNA (include the originals) would I have by the end of these ...
0
votes
0answers
13 views

To measure potentials of peripheral nervous system

I am trying to find a standardised method to measure potentials in peripheral nervous system. Just a device. I am mostly interested in forearm because little activities to interfere the measurement. ...
0
votes
1answer
19 views

Neuron Growth and Regeneration

Are nerves and/or neurons able to grow and repair after damage, but not regenerate? Is this specific to humans? I have heard of people being paralyzed, but after much therapy are able to use their ...
0
votes
0answers
6 views

Differences in Karr cell model and E-Cell

Recently Karr et al. published in Cell their model of Mycoplasma genitalium and published the complete code. Also, the E-Cell project allows to simulate a complete cell. Can anyone comment on ...
5
votes
1answer
126 views

How does the cornea breathe during sleep?

The cornea is not supplied with blood vessels and so exchanges gases directly with the atmosphere. So how does it breathe during sleep when our eyes are closed?
4
votes
1answer
127 views

Why should plants transform glucose into sucrose before transporting it to other parts?

I've learned that plants transform glucose into sucrose before sending it into phloem. But the process seems to be complex and energy comsuming. Why should plants do it? Is it really necessary?
2
votes
1answer
30 views

Prevent biofilm formation on moist surfaces

I have an indoor fountain with lots of water. What are the most common microorganisms in this kind of moist environment, and what are the standard way to prevent biofilm formation by them? I thought ...
2
votes
1answer
22 views

Why does the 'Positive-inside Rule' exist?

Gunnar von Heijne's Positive-inside Rule seems to have been around for a couple of decades and underpins a lot of what we know about transmembrane topology. It is used to predict the topology of a ...
3
votes
1answer
22 views

What's up with all the vague protocols? [closed]

I have lost count of how many protocols I've seen, including those supposed to be professionally written (such as manuals that come with kits from well known brands, or methods sections of papers in ...
1
vote
1answer
20 views

Ebola immunosupression and infection

I was wondering... If Ebola attacks immune cells and causes immunosupression, then shouldn't we see death in Ebola patients due to secondary infection?
7
votes
1answer
2k views

Can a human acquire a memory of an animal? [closed]

I would like to know if it's possible for a human to acquire an animal memory. I mean, animals store their memories in their brain, right? And so do we, humans, store our memory. So, is it possible ...
2
votes
3answers
47 views

Increasing/decreasing signal intensity in Western blotting

So I was in class and my professor was explaining western blotting. It seems as though there's more than one way to increase or decrease the signal intensity. He presented us the challenge to discover ...

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