1
vote
0answers
33 views

Common Metrics of Assessing DNA Sequence Quality

What are commonly accepted metrics for assessing DNA sequence quality (platform-specific answers are fine)? I am relatively new to this topic, and I want to either find or code (in Python or C++) ...
2
votes
2answers
51 views

When glucose production is low, the brain turns into keto acid as an energy source, how does this work?

Can someone very generally describe how the brain consumes keto acid when blood sugar has been completely depleted?
1
vote
1answer
22 views

What is the so called “hepatic glucose production”

I came across this term in my readings but I do not understand how this works. Can someone direct me to some reference as to what this "hepatic" glucose production is all about?
0
votes
1answer
35 views

Survival with only water but no food [duplicate]

How long can the average, healthy adult human survive with only water?
1
vote
1answer
29 views

Given an EC50 value, how do I reproduce the sigmoidal curve from which this was calculated?

All-trans retinoic acid (atRA) is a potent ligand for a nuclear receptor called retinoic acid receptor alpha (RARa). The concentration of atRA at which RARa is half maximal is 19nM. The dose-response ...
1
vote
2answers
30 views

Can leukopenia in a person with an otherwise normal immune system lead to non-infectious diseases in the long-term?

If a person has developed leukopenia as a side-effect of long-term use of anti-convulsant medications and his/her immune system appears normal otherwise (does not get infections any more often than a ...
1
vote
0answers
10 views

Why is there only adrenoreceptors and no active adrenergic innervation in bronchus and uterus?

Our bronchus and uterus has beta adrenoreceptors, but they have no active sympathetic nervous system innervation in these organs. Was there a sympathetic innervation in trachea and uterus, earlier, ...
0
votes
0answers
40 views

Where to find gene manipulation video (or photos)

I hope Im not asking a dumb question! Where can i find video of gene manipulation in action under microscope? Like extracting a gene from a cell, fixing gene mutations, putting the gene in a vector, ...
4
votes
2answers
25 views

Transformation efficiency

I accidentally spun down the cells after heat shock treatment before adding the media into the tubes while doing transformation? will the transformation work?
1
vote
0answers
12 views

Liver - Regeneration in Cirrhosis

Liver is the most resilient of the human organ (on par with or next to skin). A very interesting experiment on liver regeneration is here. Even if two-thirds of the liver is removed, the remaining ...
1
vote
0answers
53 views

Swapping genes?

So, gene therapy is to take out a gene, correct its mutation, and put the corrected one back into the organism, right? Is it also possible to take out a gene from an organism and put in a totally ...
3
votes
1answer
20 views

DNA content doubling in interphase

Why does the DNA content of a cell get doubled in interphase? Why doesn't it become tripled or quadrupled? What's stopping it from doing so?
0
votes
2answers
31 views

Cellular Immunity Response

What response of cellular immunity would appear after complementary protein activation to keep pathogenic bacteria away from our body? Notes: I do appreciate your explanation. Though I am facing ...
4
votes
0answers
27 views

Case Study and speculations of Edward Mordake

I am very interested in the case of the man named Edward Mordake who lived in the 19th century. In particular, he had two faces. If you have not heard of this man, please, search this up as there are ...
4
votes
1answer
36 views

How can human infants express chymosin with only a pseudogene at their disposal?

I read on the Wikipedia article about Chymosin http://en.m.wikipedia.org/wiki/Chymosin It stated that chymosin is produced by gastric chief cell in human infants. But it also stated that human only ...
0
votes
1answer
29 views

ratio between heat transfer coefficient and thermal conductivity [closed]

Consider the Robin boundary condition for the diffusion/heat equation $\mathrm{u_t=a(t)u_{xx}+f(x,t)}$: $$\mathrm{-k(t)u_x(0,t)=h(t)u(0,t)}$$ or $$\mathrm{u_x(0,t)+\frac{h(t)}{k(t)}u(0,t)=0}$$ ...
6
votes
1answer
45 views

Why differentiated cells can't “undifferentiate” (under normal conditions)?

In a process called differentiation, the cells of the developing organism undergo huge changes, which result in cells, functioning "completely" differently. Two cells are considered to be of different ...
1
vote
0answers
40 views

Closing a Plasmid by Ligation

I have been having trouble with what should be a fairly simple ligation. I had a plasmid that I needed to cut part out of, so I designed a couple PCR primers to do that. The plasmid already had a ...
2
votes
0answers
29 views

Do The Traces Genetic Diseases Remain in families?

I know that there are certain diseases that are predominant on genes. But, is there any sort of surety that if parents are suffering from a disorder then their offspring has to suffer from the same. ...
6
votes
1answer
29 views

What Goes on in Photosystems at the Molecular/Atomic Level?

In light-harvesting complexes, how is the energy of a photon transferred from one pigment molecule to another? And in reaction-center complexes, how does the primary electron acceptor capture an ...
7
votes
1answer
498 views

Identifying an insect looking like a crumle

Recently a little insect crawled over the carpet. First I did not notice, as its appearance is quite similar to the coloring of the carpet. Then I understood that it is not a rolling crumble, as the ...
3
votes
0answers
23 views

Blood as newtonian flow and Poiseuille law [closed]

For a prep exam: A healtly pacient has a carotid with the following characteristics: intern ratio: 𝑅= 2.8 mm, 𝑃2 = 89 π‘šπ‘šπ»π‘”, 𝑃1 = 90 π‘šπ‘šπ»π‘”, πœ‡blood = 3.5 cp, 𝐿=10.0 cm. Using the ...
2
votes
1answer
33 views

How to define drug-resistant or -sensitive cell line when knowing the IC50 values?

I have got the IC50 data for a drug on different cell lines. How to define if the cell line is sensitive or resistant towards this drug? Could anyone tell me how to define this?
-1
votes
0answers
21 views

which microscope and approach best for bacterial, fungal, yeast, mold research [closed]

I am looking for the best approach to look/identify bacterial, yeast, fungal, and ideally biofilm synergy, coming from mucus membranes for allergy and infection research. Budget is around 3K. ...
2
votes
1answer
31 views

Genetic Imprinting and Cell differentiaion

It does not seem possible that these two processes can coexist: 1) Genetic imprinting is the phenomenon where genes are expressed differently depending on the parent of origin: 1a. Methylated ...
5
votes
1answer
374 views

What produces the strong odor of decaying dead animals?

Are there specific chemicals responsible for producing the strong, putrid odor that is present after an animal deceases? Also, how long does this odor last?
0
votes
1answer
17 views

Beta oxidation of saturated fats

In my text book there is an example of a 18 carbon long fat and it says that it can run 8 laps of oxidation. I don't understand why it doesn't run 9? 2 are removed each cycle? Thanks.
0
votes
0answers
25 views

Why do four spermatids have different genomes?

It's been a while since my last class in genetics, so I've forgot most of it. However, I found myself studying human reproduction and I've the following doubt concerning spermatogenesis: the process ...
1
vote
1answer
32 views

Is the motor cortex identical to the sensorimotor cortex?

As far as I understand, the primary motor cortex (M1) and primary sensorimotor (SM1) are notations for the same cortical area in the brain. Am I right that there is no dedicated motor cortex, and that ...
1
vote
0answers
28 views

Compare Proteins - Structural / Sequence parameters

I have been working on algorithms that extract co evolutionary signatures from protein sequence. As a result of my work I got some evolutionary information which possibly explains cotranslational ...
2
votes
1answer
55 views

Are genotypes with the same two alleles equivalent even if the alleles come from different parents?

I am looking at the following question If $m$ alleles may occur at a given locus, how many distinct diploid genotypes are possible at that locus? The obvious answer is that there their is $m$ ...
0
votes
0answers
21 views

What are comparative advantage of different NGS platforms?

Sanger and Maxam-Gilbert introduced first two methods to sequence DNA. Sanger's Method had great comparative advantages and led the sequencing projects in its time. As I know, currently we have three ...
3
votes
1answer
51 views

Hardy-Weinberg Color-blind

In a city, 4% of male population have color blindness. How many of the female are (a) color blind carrier, (b) color blind? Suppose the city holds Hardy Weinberg equilibrium. My progress: 4% of male ...
0
votes
1answer
26 views

What pattern can be learned from the data of RNA seq counts and HiC matrix? [closed]

I now have some data on the RNA seq counts and related Hi-C matrix of gene segment on a chromosome. My concern is, basically, what can we do with these data so as to establish the connection between ...
0
votes
1answer
34 views

Can we use the HIV antibody test to detect HIV in newborns? [closed]

Adults and elder children can use HIV rapid test to test HIV infection. Can we use the HIV antibody test to detect HIV in newborns too?
1
vote
0answers
18 views

Why is Pilobolus phototropic?

Pilobolus is a fungal genus whose fruiting bodies consist of a stalk ending in a spore mass that is launched via turgor pressure to disperse the spores. The fruiting bodies grow towards light, which ...
1
vote
0answers
17 views

Question about cytoskeleton coordination

I am trying to study for a biology and I am having some confusion over the following topic. Can anyone help explain/ shed some light on the concepts of Rho family GTPases. Is it true that we have Rho ...
7
votes
1answer
48 views

Reasons for the HIV-1 epidemic

So, the HIV-1 jump to humans occurred as early as the 1920s, but the AIDS epidemic didn't start until the early 1980s. Some things I don't understand about this: Why the delay? What is needed for a ...
4
votes
0answers
77 views

Phylogenetic method to detect shift in mean of continuous variable

I am currently working on a project where I study the change of a continuous morphological variable with body size (measured as body weight) across diverse taxa (insects, spiders, lizards, frogs). In ...
1
vote
1answer
37 views

Why use creatine phosphate?

We use creatine phosphate as an energy storage to resupply ADP with a phosphate group as our muscle cells only contain about 2-5 mM ATP. But why doesn't the muscle cells just keep 20-30 mM ATP instead ...
0
votes
0answers
25 views

Why doesn't the body optimize how many fatty acids it burns?

Ketone bodies are produced due to an excess of fatty acids being burned (accumulation of acetyl-CoA) so my question is: Why doesn't the body simply regulate how many fats it burns so it doesn't have ...
5
votes
2answers
37 views

Time required for RNA precipitation in ethanol

I precipitated bacterial RNA during 1 hour at -80ΒΊC after depleting rRNA with Ribo-Zero kit. Does more time lead to better results?
2
votes
1answer
29 views

How are ketone bodies used?

While searching for literature on ketone bodies, I can only seem to find how they are synthesized, but not how they are broken down. I'm looking for the series of events with enzyme names and ...
9
votes
1answer
269 views

Why gluconeogenesis?

It seems quite pointless to me. Why would I want to use energy to create a molecule that I can then use again for less energy? It seems only to serve as a complete waste of energy to me? Can someone ...
2
votes
1answer
26 views

NADH shuttles - Why cytosol?

Why is the malate-aspartate and the glycerol-3-phosphate shuttles located in the cytosol and intermembrane space? The krebs cycle happens in the matrix so why wouldn't they push electrons from the ...
5
votes
2answers
42 views

Why is 5S-rRNA is different from other rRNAs in place of transcription and usage of RNA polymerase?

While transcription of rRNAs happens in nucleolus mediated by RNA Polymerase-I, we see that 5S-rRNA is transcribed elsewhere by RNA Polymerase-III. What is the cause and why?
2
votes
1answer
18 views

How is circadian rhythm of gastric acid secretion regulated?

Gastric acid secretion shows a circadian rhythm where acid production in the morning is minimal and it reaches a maximum towards the evening. How is this process regulated?
3
votes
0answers
27 views

What percentage of cells in the human body are infected during a non-fatal viral infection?

I understand that this would be a very rough estimate, but approximately how many cells in the human body are infected during an average non-fatal viral infection before the infection is eliminated by ...
1
vote
1answer
126 views

What is the chemical composition / empirical formula of Herpes Simplex 1?

Viruses such as polio are so well documented that a search for "empirical formula polio" gives you something like ...
1
vote
0answers
37 views

Further progression with neuroscience education [closed]

Question for you I hope this is in the right area and all if not please advise on where to put it, Having taken biological neuroscience for a year now as a masters program I feel like I want to go ...

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