5
votes
2answers
34 views

How do you visualize RNA on a gel?

I have run an in vitro transcription reaction and produced some RNA of a single species and definite size. I would like to visualize it to check if the reaction worked. Can I do this on an agarose ...
6
votes
1answer
169 views

Ebola: cleaning of protective suit

As far as I know, in developed countries protection suits used by people who treat patients with Ebola are disposed of after a shift. From a rather ambitious costume last year, I still have a hazmat ...
1
vote
0answers
17 views

Does drinking spoiled milk that has been boiled upset the GI tract?

With water, boiling will kill harmful pathogens. Does this also hold true for milk ? If it does not, what in the milk will continue to cause gatrointestinal distress ? Shouldn't boiling kill the ...
3
votes
0answers
69 views

What are the psychodelic effects of (stellar) anise and how many people are affected?

Backstory: My girlfriend reacts heavily to chocolate and drinks containing stellar anise, in a way that seems comparable to psychodelic drugs. After consuming it, it takes approximately five minutes ...
3
votes
1answer
35 views

What equation to compare protein isoforms in a Western Blot?

The protein isoforms I am interested in comparing appear as distinct bands on the gel I have already run. I have an Excel sheet with optical density measurements I obtained using ImageJ; it looks ...
4
votes
2answers
165 views

Why proteinase K doesn't degrade itself?

Can anyone tell me why proteinase K doesn't degrade itself? If possible please provide me the source.
2
votes
1answer
52 views

How do animals handle light refraction in different media?

For a human, when diving underwater, everything is blurry because of the water's different refractive index compared to that of air, which we are accustomed to. How do animals, like turtles, seals or ...
-2
votes
0answers
32 views

What's the difference between meth and mdma so that the effect are vastly different?

One is methylene deoxy methampetamine. The other is methampetamine. They both contains methamphetamine. So why MDMA is far saver than meth?
2
votes
2answers
49 views

Are the DNA codon mappings arbitrary?

I am a Biology novice so I apologize in advance if I state anything incorrectly.... My question is, are the DNA codon mappings arbitrary? I understand that the DNA code is made up of codons which ...
2
votes
0answers
23 views

What is the allelic frequency at mutation-selection-drift equilibrium?

At mutation-selection equilibrium the expected frequency $f$ of a given locus is $$f ≈ \frac{\mu}{s\cdot h}$$ , where $\mu$ is the mutation rate, $s$ is the selection coefficient and $h$ is the ...
0
votes
0answers
14 views

Which trees/shrubs provide most food for wildlife in northeastern US?

Let's say I have an opportunity to plant a couple trees or shrubs in my backyard that is adjacent to a small 20 acre area of woodland surrounded by urban development. There are animals like rabbits, ...
1
vote
1answer
22 views

How does fluorouracil inhibit pyrimidine synthesis?

I was reading in my pharmacology textbook about fluorouracil, and my book mentioned that fluorouracil is an antimetabolite whose function is to inhibit pyrimidine synthesis. However, I was curious to ...
6
votes
2answers
62 views

Just how dangerous is ethidium bromide?

A common precaution for preparing EtBr-agarose gels is to add EtBr only after the gel solution has cooled somewhat, because EtBr is a dangerous mutagen. Supposedly, adding it to a hot liquid allows ...
8
votes
1answer
187 views

How do I put on a labcoat?

Let's assume I am wearing a labcoat for two reasons: Prevent the various bacteria, proteins, skin cells and substances on my clothes and my skin from contaminating my experiments. Prevent various ...
0
votes
0answers
11 views

Open Source Variant Caller comparable to GATK Haplotype Caller?

Is there a Variant Caller comparable algorithmically and in performance to GATK Haplotype Caller but Open Source Compliant (non-restrictive use)?
0
votes
1answer
26 views

What's the evolutionary reason behind decussation?

A bunch of stuff in the human nervous system decussates. Optical information inputs from the eyes cross over in the optical chiasm. Multiple sensory and motor pathways cross-over before ascending the ...
0
votes
1answer
34 views

Fruit colour related to the dissolving of pectin

What does the dissolving of pectin have to do with the colour of a fruit?
4
votes
1answer
172 views

How many RNA-binding proteins can simultaneously bind on a single mRNA?

Typically, how many RNA-binding proteins can simultaneously bind to a single mRNA? Or said differently, how many "binding sites" does an mRNA have? What order of magnitude? I am interested in RNA ...
1
vote
0answers
64 views

Are humans the only living species that have the concept of exercise? [closed]

Humans have the concept of working out and exercising to keep themselves healthy. I have not heard of any other species that does the same, but I am just being ignorant. Are humans the only living ...
8
votes
1answer
224 views

What's the difference between growing cells in culture and cloning them?

The wikipedia page on Hela cells refers to George Gey being able "to isolate one specific cell, multiply it, and start a cell line." Later it says, "In 1955 HeLa cells were the first human cells ...
3
votes
1answer
54 views

About sharks and how they find fish

Other than olfactory senses do sharks use some kind of sense that uses electromagnetic waves? Is this similar to an electric eel? I saw a book with the cover statement 'Sharks never get cancer'. IS ...
4
votes
2answers
55 views

Is chloroplasts per cell indicative of the photosynthetic power of a plant?

Plant-A has on average N chloroplasts per cell. Plant-B has on average M chloroplasts per cell. If N > M, is plant-A more efficient in turning carbon dioxide into oxygen? Also, is it possible to see ...
1
vote
1answer
29 views

PSI-BLAST website algorithm parameters

http://blast.ncbi.nlm.nih.gov/Blast.cgi In this website, when I want to apply the psi-blast algorithm on a sequence, under the section of algorithm parameters , what does PSI-BLAST threshold mean? ...
2
votes
1answer
67 views

How does an embryo know where to grow limbs etc

For example you have a cell or already a bunch of cells. Those cell(s) divide and after several week you have a grown organism, for example a human with limbs, several different organs etc. However, ...
2
votes
0answers
56 views

How many proteins are synthesized in human body throughout life?

My idea to calculate this is: Define up to what age will do the calculation. (for instance 60) Determine how many cells born and died up to that point in life. (by type of cell) Determine how many ...
0
votes
1answer
47 views

Whats the difference between voltage gated and ligand gated neurons? [closed]

How are they similar and how are they different?
2
votes
0answers
47 views

What makes living beings alive? [closed]

To clarify, I'm not asking about the common properties of living organisms, such as reproducing and having a metabolism, etc. What I'm curious about is, what is the difference between organic ...
0
votes
0answers
31 views

What is a good source that will help me classify protists?

I am a 15 year old that enjoys looking at the micro world. The only issue that I have is that I can't find a good online source that will help me classify the name of the micro organism that I'm ...
2
votes
2answers
82 views

To get Standard ECG from PhysioNet?

I am trying to get standard ECG by using PhysioNet's ATM with parameters but I get It should be something like How can you get complete standard 12-lead ECG out of Physionet database?
1
vote
0answers
25 views

Why does K+ have more concentration inside of the cell compared to outside of the cell? Why is Na+ and Cl- the opposite?

Can someone tell me the fundamental reason why K+ has low concentration outside of the cell and more inside of the cell?
0
votes
1answer
40 views

Can anyone remind me of the name for the inter-scapular space on the back?

It's one of those names which are often said not to exist, like philtrum. Thanks
4
votes
1answer
33 views

protein-binding sRNAs

I am actually a computational biologist, pardon me if my question description is a bit off-note, my curiosity is driving me on A bit of background and context: In bacteria, a regulatory protein ...
12
votes
1answer
67 views

What is the mechanism responsible for the periodicity of hiccups?

Hiccups (singultus) are caused by involuntary spasm of the intercostal muscles and diaphragm, producing a sudden inspiration with associated glottic closure. It seems the neurobiology of the reflex ...
2
votes
0answers
83 views

Questions about insects eating flour and living in the cracks of a wooden cutting boards

I have a large wooden cutting board which is used in kitchen mainly for preparing egg pasta. The wood type is Populus (poplar or aspen or cottonwood). The main ingredients used with the cutting board ...
1
vote
0answers
39 views

Possibility of Human cloning

Techonology has evolved so much as to clone animals. But, what about human cloning? Is it really possible to create humans in lab? Or is it that technology has not advanced to that level..? Why cant ...
2
votes
0answers
17 views

What is the scientific opinion on plus lens therapy?

Plus lens therapy is the theory that most myopia is environmentally triggered and that conventional corrective minus lenses increase nearsightedness over time. Thus the hypothesis that applying plus ...
2
votes
1answer
18 views

Mechanisms of Flagella

I know that dynein arms and microtubules are involved in the contraction of certain parts of the flagella to produce a wave motion, but I don't understand they are related to the 9+2 structure of the ...
3
votes
3answers
85 views

Stardust or the elements in our bodies

How do we know we are made of stardust? As our cells divide, are our atoms repurposed from existing materials or spontaneously generated? Do we consume materials after we are born that contain ...
4
votes
2answers
33 views

What happens when you mix type A with type B blood (and v.v)

What happens when you mix different blood types that are not compatible? For example, if a patient has type A blood and receives type B blood, or v.v.?
2
votes
2answers
97 views

are the spontaneous generation and evolution the same idea but in a different time scale?

I was in my biology class, learning the history of spontaneous generation and how this idea was discarded. I told my teacher that is the same as evolution but in a different timeline. I Argue that ...
1
vote
1answer
30 views

programmatic fast search of 100-1000 short reads to a public server and obtain list of nearby genes

What are the options for programmatic fast searching of 100-1000 short reads to a public server and obtain list of nearby genes where the reads map? Input: ~100-1000 short reads Output: GFF list of ...
2
votes
0answers
24 views

Can Galactose be synthesized with photosynthesis?

I know that galactose can be found in sugar beets and some gums so I thought plants could synthesize galactose but in my book the answer to the question "Which of these can be synthesized with ...
2
votes
1answer
38 views

How to identify genes?

You and me are different at DNA level. My eye color gene is different from yours. So, my DNA is different from yours. How can a scientist identify a certain gene in a chromosome (and its function) if ...
0
votes
0answers
26 views

How TCGA CNV values are calculated?

When I download CNV SNP array data for ovarian cancer from TCGA data portal, I see some very small numbers like -5, -6 in the "Segment_Mean" column of the segmentation data files. I am very new to ...
3
votes
1answer
75 views

Is ebola non-contagious until symptoms appear? Why?

There's been a lot of talk lately about the man who recently came down with ebola in Dallas, Texas. While I live elsewhere, I keep hearing quotes from CDC and state officials that ebola is not ...
9
votes
1answer
635 views

Horned Mohawk looking insect. What is it?

Any ideas? I didn't know any other way to explain it. I live in Indiana, USA if that helps. Someone said it might be a "stink bug" but I'm not sure and not willing to find out!
0
votes
0answers
26 views

Calculate generations since divergence

Can the formula $\mu = k/(2t + 4Ne)$ be used to calculate the divergence time for any two species given a mutation rate, generation time and sequence divergence value?
4
votes
2answers
210 views

What determines the autumn leaf color?

It is autumn out there. Green, brown, red, yellow, and mixed-colored leaves drop from the trees to the ground. What determines the colors of the leaves? Wikipedia tells me Leaf senescence is the ...
1
vote
0answers
53 views

Suggestions of famous experiments with plant cells? [closed]

I am looking for examples of famous experiments with plant cells. Ideally, the experiment would use: bright-field microscopy staining include several compared groups, so that e.g. one-factor ...
7
votes
1answer
58 views

Can you get enough water by eating only fish?

Scenario: In a boat in the middle of the sea, no freshwater or food stores, no desalination equipment, no rain, but you can catch fish and eat it raw. Can you get enough water this way to survive, ...

15 30 50 per page