0
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0answers
19 views

Are HEK293 cells a positive control for wild type p53 expression?

I have searched in the web and found that these two antibodies from abcam use hek293 as positive control. * ab154036 * ab1101 But I am using another antibody: * AM11182PU-S (OriGene Technologies Inc.)...
1
vote
0answers
29 views

How well can proteins discriminate between ATP and GTP? Can ATP act as a GTP mimetic?

GTP and ATP are similar structures with the adenosine and guanosine groups differing. Both are involved in a vast array of biological functions. However it has been shown that in certain cases, GTP ...
0
votes
0answers
48 views

Do male humans have a mons pubis?

Wikipedia writes: In human anatomy, and in mammals in general, the mons pubis (also known simply as the mons, and known specifically in females as the mons Venus or mons veneris), is a rounded ...
1
vote
1answer
37 views

Can salt-water frogs survive in normal water (A well)?

I don't know much about frogs, but there seem to be salt water frogs. 1) Are there such things as salt water frogs? 2) Are there frogs that live in the sea? (in reference to Vivekananda's quote) 3) ...
3
votes
1answer
90 views

When was it discovered XY chromosomes decide the sex of a child in humans?

Several stories are told from before-genetic-age (books and movies are my reference, the one present in my mind is Marie-Antoinette by Copola) in which we can always see that women are blamed for ...
0
votes
0answers
17 views

Can sugar alcohol be replacement of sugar in cola or soda?

I have read that cola in a can contains about 8 tea spoons of sugar. Can some of them be replaced by sugar alcohol such as xylitol or sorbitol? Because sometimes drinking soda is helpful to quench ...
4
votes
0answers
25 views

What is the conical mouthpart of a soldier termite and what does it do?

I'm trying to get a visual understanding of termite anatomy and I'm getting quite confused on the protruding mouthpart area (it looks like a beak) located between the pincers. What does it do / what ...
0
votes
0answers
5 views

Delta Mye and F/F after gene name

What do the delta Mye and F/F mean? For example in the following figure (https://www.researchgate.net/figure/45695903_fig5_Figure-2-Enhanced-plasma-inflammatory-cytokines-in-GRK2mye-mice-Mice-were-...
0
votes
0answers
22 views

Why do I drop my pen in a drowsy lecture?

I know that there is loss of muscle tone during REM sleep. Is this loss in muscle tone the reason I generally drop my pen in a sleepy lecture? But we were taught that sleep has 4 stages and REM sleep ...
0
votes
1answer
19 views

DNA Sequencing Types & Qualities

I've been searching for a list of the types of DNA sequencing (e.g. Sanger, Next-Generation) and how prone they are to sequencing errors (None, Somewhat, Very), but I haven't been able to find ...
0
votes
0answers
22 views

Can someone identify this snail? Thanks

Found in an almost dried out brook in Eastern Massachusetts. This is the lowest the brook has been in anyone's memory.
1
vote
0answers
10 views

Membrane resistance of a neuron

I am about to simulate a neuron activity with the "Leaky Integrate and Fire" neuron model. But for that I need the membrane resistance. I was really looking a lot online, but I just cant find a value. ...
0
votes
1answer
14 views

Allele-specific bisulfite sequencing

When evaluating methylation status at various CpG sites after sequencing, how much consideration should one give to random single base pair insertions and deletions. Suppose there is a CA dinucleotide;...
1
vote
1answer
50 views

In Darwinian evolutionary theory, does the environment has any role? [closed]

In Darwinian evolutionary theory, does the environment has any role? To state it clearly: Suppose I have an isolated system. First, I place several types of entities (organisms) in an environment. ...
0
votes
0answers
15 views

What are chemical candidates for replicator molecule?

I have read Selfish gene by Richard Dawkins and idea is that at random some molecule was synthesized that had a property if there is enough 'materials' to construct ...
0
votes
1answer
62 views

How did humans in different regions evolve so differently? [closed]

Google is about as helpful as a mouse trying to catch a piano, since it's listed websites that aren't very clear or informative. So: How did humans evolve to be so different from one another? Say, ...
1
vote
0answers
10 views

How can one measure the effect of a stem cell's matrix on the progeny of that stem cell?

A number of recent papers that have used hydrogels to alter the ECM of mesenchymal stem cells to quicken their development to cells used specifically for cartilage or really any other type of cell. ...
1
vote
0answers
26 views

“Genotype structure”?

What does a geneticist mean when they write "genotype structure", e.g. the sentence "...represents continuous allele frequencies for both tumor and normal samples, while leveraging the expected ...
1
vote
0answers
7 views

mtDNA sequencing error rates

What is the method to estimate the error rate in mtDNA sequencing? E.g. we are given a profile like this: 16069T 16126C 73G 152C Is there a method, a table of known values, or any other way to say ...
0
votes
0answers
24 views

Distance between P5 and custom read 1 primer for Illumina HiSeq sequencing

I have a minigene library which is amplified with P5/P7 sites that looks like this [P7/barcode][constant region][ variable region ][18nt constant region][SP1][P5] (436nt total) We sequenced this ...
1
vote
1answer
24 views

Calling Alterations in Exome Data

What are the main technical differences between accurately calling somatic point mutations vs copy number variation (CNV) in exome data? Side note: would you need other -omic data to accurately infer ...
0
votes
0answers
17 views

Genetic screening understanding requisites

I am a mathematics and philosophy undergraduate student studying a bit of a biology as part of a philosophy research project on genetic screening. I have not studied biology since I was 16 and I was ...
1
vote
1answer
58 views

Why are bald heads are so “shiny”?

Why do males with baldness have shiny skin on their head, whereas we don't experience such "shiney skin" in other parts of body without hair.
-1
votes
0answers
14 views

Can LC-MS/MS and MRM act as a complementary to RT-PCR, Western blotting and ELISA?

I am working on protein quantification using Liquid Chromatography-tandom Mass Spectrometry with Multiple Reaction Monitoring (LC-MS/MS MRM), I am not sure if it can act as a complementary to RT-PCR, ...
-1
votes
1answer
29 views

Can ants survive a plane ride?

I just came back home a few hours ago from the airport and my aunt had peaches in her car full of ants. This made me wonder if the ants had joined us from the plane? This leads to the question can ...
2
votes
0answers
27 views

Do any non-human species have packed abs?

The classic "six-pack abs" in humans is due to tendons stretched across the abdominal muscles and low body fat. Do any other animals have a similar arrangement of tendons that could result in visible "...
3
votes
1answer
47 views

Damselfly identification

Can somebody identify this damselfly? Found near a stream about 15 miles west of Boston.
1
vote
1answer
34 views

Is every protein coded for by one gene or can it be coded by more than one?

The common knowledge is that one gene codes for one protein. There is a one to one correspondence. But are there proteins that can only be assembled from several genes?
4
votes
0answers
35 views

Are there diseases for which a placebo treatment is state of the art?

I was reading about the placebo effect, and I wondered if there exists or ever existed a disease with the following properties: There are no known treatments for the disease that perform better than ...
1
vote
1answer
34 views

Can RNA be extracted from tissue suspended in formalin?

There are two tumor samples floating in a 10% formaldehyde solution (i.e formalin). Is there a protocol for RNA extraction under these circumstances? I am concerned that using the protocol for ...
3
votes
1answer
69 views

Can anyone identify this dragonfly spotted outside of Boston, MA?

Can somebody identify this dragonfly please? It was seen about 15 miles west of Boston.
0
votes
0answers
8 views

Collecting Specific Microorganism Specimens Under Microscope

What are options for collecting individual specimens beneath a microscope? I have been looking at needled syringes on amazon, however I am not sure what gauge to be looking at before making a purchase,...
1
vote
1answer
69 views

What is the evolutionary advantage of having pubic hair?

Only humans have pubic hair. What is the evolutionary advantage in having it?
3
votes
1answer
24 views

Can a cleaved plasmid “close” without exact matching “sticky ends”?

Say if you have a multiple restriction site and use two restriction enzymes on it to cleave a plasmid, can it recombine with ligase? My concern is that without the small sequence of nucleotides ...
0
votes
1answer
35 views

Is there a difference in the energy produced from the hydrolysis of GTP and ATP?

Is the energy produced from the hydrolysis of GTP greater than/less than or the same. I know that GTP can be converted to ATP through a pathway and so my thinking was that GTP would be less.
1
vote
1answer
57 views

what do I need to use for protein protein interaction network

I would like to know what i should use to be able to build a PPI network. I have seen different structure for different techniques. for example sometimes the input look like this , each protein with ...
0
votes
0answers
12 views

Relative difficulty of plant cell culture vs yeast on Industrial scale

I often read about industrial synthesis of an originally plant derived secondary metabolite (e.g. Artemesinin) and it is mostly done via fermentation with transgenic organisms optimized to express the ...
4
votes
1answer
75 views

Reason behind using inducible promoter for heterologous expression

Protocols often seem to use regulatable promoters when expressing a heterologous gene in a host like yeast where they add something like IPTG to induce expression from (say) the lac promoter. Is ...
2
votes
1answer
77 views

What chemicals/substances exist in green capsicum (peppers) that do not exist in red capsicum?

I have known 3 people in my life that have incredibly adverse reactions (one called it an "allergy" and his symptoms did resemble such) to consuming any form of green pepper (capsicum) but have no ...
-1
votes
0answers
10 views

Question on my Bradford Pear Tree [migrated]

We have 2Bradford Pear trees out front,this spring the one on the right was full of Bloom's,the left one was very scarce of blooms, the right tree is full of leaves,the left one is almost bare. We are ...
0
votes
0answers
30 views

What's this insect?

Found on my indoor egg plant. I also have aphids, spider mites, and psyllids on it, and am hoping it's a predator like a ladybug larva but the colours are wrong. It's about 6 mm long. Found in New ...
0
votes
0answers
5 views

How common is it for environmentally occurring bacillus strain to contain some sort of plasmid?

I am designing a method for testing whether two new bacillus isolates, that are ionic silver resistant, store the silver resistance mechanism on a plasmid or on the chromosome of the bacteria. In my ...
0
votes
0answers
21 views

map list of proteins into an already built network

I have a built reliable network which can be downloaded from here http://www.filedropper.com/mynetwork This network is bulit using Cytoscape. Now I have a list of proteins IDs, for example ...
0
votes
2answers
33 views

How does sodium ion increase blood pressure?

So I have been reading about the RAAS system and I was wondering how natrium (sodium ) ions increase blood pressure.I have been looking up on the internet and have yet to find an answer.Help
0
votes
0answers
6 views

Which of the following is an accumulation and release centre of neurohormones?

The options are : a)posterior pituitary b)hypothalamus According to me, it should be 'posterior pituitary' because though neurohormones are produced in hypothalamus but they are temporarily stored in ...
0
votes
1answer
15 views

Enzootic vs Epizootic?

I'm studying microbiology and I see these words - epizootic and enzootic, often but there are no clear explanations for them online. Can someone help please?
0
votes
0answers
24 views

Are there any bacteria with a protective calcified ‘skeleton’?

Some algae like the coccolithophores form coccoliths which are individual plates of calcium carbonate. But are there also bacteria known that can make such a skeleton?
1
vote
0answers
24 views

clades on a tree - biopython

this is the situation: Let's suppose we have an alignment. When a phylogeny tool (PhyML in this case) is used on this aln, a tree consisting of 5 clades is returned. The thing is: suppose we have ...
0
votes
0answers
31 views

Genus names unique?

I have read that binomial names are unique, and that species in different genera can have the same species epithet. Are genus epithets unique? That is, can two different families have genera with the ...
0
votes
0answers
11 views

History of species identification before 1930 [migrated]

I am trying to research how new species have been recorded over time, but specifically in the period before the 1930s. Where would an academic who had discovered a new species get their discovery ...

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