3
votes
1answer
20 views

Changing time and rpm of centifuge

Inspired by this question, I want to ask a question about centrifugation. Suppose a protocol says : 10 min at 2500 rpm . Can we instead centifuge for 5 min at 5000 rpm or 20 min at 1250 rpm ?
1
vote
1answer
40 views

Missing 4$H_2O$s (per glucose) in Cellular Respiration… Where can they be?

I having trouble understanding the equation of the cellular respiration. The thing that bothers me is the number of $H_2O$ molecules. Generally, cellular respiration is written thus : $C_6H_{12}O_6 + ...
1
vote
1answer
43 views

What is the biological reason that some viruses cause sore joints (eg flu)?

Frequently I have experienced sore joints during a bad bout of influenza. I understand that during time of sickness, white blood cells are deployed in the blood stream. I also believe that white blood ...
1
vote
2answers
20 views

Concerning Apoptosis

What if cellular growth and repair is 'forced' to occur repeatedly in a region where it wouldn't normally happen , if the biological area was 'healthier'. Could this more aggressive cellular growth ...
3
votes
2answers
49 views

Is half-a-tablet of X a substitute for a tablet of X/2?

This may be anecdotal. At the pharmacy earlier, the prescription called for a drug with X mg of the target chemical. The pharmacy however only had stock of a higher potency of the same brand. E.g. ...
5
votes
0answers
28 views

E coli cotransformation efficiency

What is the E coli transformation efficiency for 2 plasmids? Are any studies that have looked at the correlation between number of plasmids transformed and transformation efficiency? Is there a ...
3
votes
1answer
38 views

Does yeast grow on LB agar plates?

I would like to grow E coli cells on cellophane on top of LB plate, and then grow yeast cells on the same LB plate. Does (or how well) yeast grow on LB plates? Is there a composition media that can be ...
1
vote
1answer
34 views

Why can't my body deal with cold-sores

I am not a biologist, but I have always wondered why my body hasn't learnt to deal with the HSV-1 virus. I understand it reamins dormant for a while and things like stress or the common cold can ...
0
votes
0answers
10 views

Fibrinous inflammation, exudate and scarring in monotonous movements and ageing

I have palpated many hard organs during my work and studies. I have seen many young and old people who have hard organs, like very hard shoulder - very common. I have interviewed some of them, some ...
1
vote
0answers
6 views

Which kit provides the highest yield and purity of extracted DNA from FFPE tissue samples?

I'm looking to extract DNA for sequencing from FFPE (Formalin fixed paraffin-embedded) tissue samples. I'd like to use a commercial kit that provides a high yield of good quality DNA.
1
vote
0answers
14 views

Do we really dream multiple time a night?

So Ive heard that when we sleep for a good 8 hours we dream quite a few times? But we only remember very little of them if any, is this true? How is it known that multiple dreams occur if most of them ...
3
votes
0answers
30 views

Burns' “To a mouse” - what species of mouse?

What's the most likely mouse species Robert Burns is referring to in his poem "To a Mouse, on Turning Her Up in Her Nest with the Plough". Burns farmed near Ayr, which is on the Firth of Clyde in ...
0
votes
1answer
32 views

Plastid and mitochondria

I am not biologist, so please bear with me for this basic question. Although I tried googling, I am confused. What is difference between plastid, chloroplast and mitochondria? Are there any plant ...
0
votes
0answers
11 views

To visualise Mycosis, Mycogenic hypersensitivity and Mycotoxicoses?

I am trying to visualise these three things first mycosis second hypersensitivity (probably related to sepsis somehow) Mycotoxicoses (probably occur only in SIRS) I am having this figure in my ...
3
votes
1answer
22 views

Knockout Rat Phenotype Database?

Is there a database where I might list the phenotype of a certain rat knockout and/or search for other similar phenotypes? There is a International Mouse Phenotyping Consortium, which has the goal ...
1
vote
1answer
39 views

What can cause the swelling in high protein diet of Whey proteins?

I am thinking what can cause the swelling of high protein diet of Whey proteins. Liver does breaks those proteins to branched chain amino acids (BCAA), which can cause this swelling. However, I am ...
1
vote
0answers
22 views

What is the possibility of retinal damage from light flashes? [closed]

During the process of Fluorescein angiography, flashes of light (which are intense enough to make one wince) are used to photograph the back of the eyes. For several minutes after the procedure, ...
2
votes
1answer
19 views

Can SIRS occur without Sepsis from Infection?

I am thinking this figure It suggests me that there has to be Sepsis that infection can lead to SIRS. I am thinking particularly the pathogenesis of Cryptococcus neoformans where respiratory ...
2
votes
0answers
30 views

Can animals distinguish other animals?

Are animals able to tell the difference between other animals. I dont mean like a cat seeing a dog and thinking "Oh no its a dog". I mean how does prey distinguish predator or threat? Say for instance ...
2
votes
0answers
32 views

Why don't birds in flocks fly into each other?

I've seen videos of hundreds of birds flying in the same general direction but creating incredible patterns in the sky seemingly instantaneously! How do they all know in what direction to turn at the ...
2
votes
2answers
59 views

Initial population when i count backwards?

My question is dealing with how many ancestors it took to produce me: --> to produce me it took 2 people (my parents) = ($2^1$) people = 2 --> one generation further included = ($2^1 + 2^2$) people ...
2
votes
1answer
44 views

Why don't we see new diseases more often? Or, ever?

It seems like every disease we ever hear about is something that's been around since ancient times, since thousands of years ago. Of course new diseases were catalogued over the course of the past ...
3
votes
2answers
32 views

Measuring reverse transcription yield?

Is there any measurement I could perform after RT that allows me to check the efficiency that the procedure had? Nanodrop cannot be used as remnants of RNA and poly T primers mask measure, or?
2
votes
1answer
25 views

Single channel micro arrays

What does single channel microarray mean? When is it used? Thank you for your case in advance.
2
votes
1answer
14 views

How does HBeAg change to Anti-HBe in acute hepatitis

I am thinking this figure which can also be drawn like this How does HBeAg change to Anti-HBe? There are some triggers that stimulate Anti-HBe production after HBeAg level is done. I think ...
1
vote
0answers
13 views

What is the relation between HBeAG and Anti-HBc

I am analysing this picture about acute hepatitis, viral infection of HBV, where are standard antibodies and antigens of HBV: HBsAg serum antigen HBeAg some antigen in blood anti-HBc antibody in ...
1
vote
1answer
40 views

Understanding DNA supercoiling

While trying to understand DNA supercoiling, I came across these lines in the book Genetics by Ursula Goodenough : All natural DNA is superhelical. The axis of the duplex itself follows a ...
4
votes
1answer
49 views

Do cancer cells give off specific chemical signatures?

Do cancer cells give off specific chemical signatures? Are these signatures different from normal cells?
0
votes
1answer
27 views

Quantitative Traits Genetics

Can someone please help with this question? I'm not so sure whether part A should be 11 inches or something else. Also, is part B correct
1
vote
1answer
22 views

If a gene altered causes cancer and creates a protein for cancer can the new protein be isolated in some way?

In the above title question, can the protein thats altered not be isolated (to separate out from other proteins) somehow? Is there nothing that can bind to the specific cancer proteins that will not ...
4
votes
2answers
83 views

DNA of the human body?

If our DNA determines certain patterns within the human body such as hair color and eye color, if scientists were able to "turn off" such as the gene for eye color after the pattern was established, ...
1
vote
2answers
38 views

What's the fastest legal way for the nutrients in a dead body to re-enter the ecosystem?

Apologies for weirdness. Of the various legal methods of disposing of a human corpse, which one ensures the nutrients which compose the body get back into the wider ecosystem the fastest? Unless ...
3
votes
1answer
38 views

gene expression fold change threshold limit

When interpreting qRT-PCR results, which results are acceptable as fold changes, which are acceptable for housekeeping genes? e.j. a values of 0.8 raw fold changes for a "housekeeping" gene is ...
-4
votes
2answers
49 views

What would be the reaction to the flesh?

If an acid, salt, and alcohol solution were formed into a gel, what would be the reaction if injected into flesh and what would be the effect on the skin say for rough and cracked feet, acne, newly ...
1
vote
8answers
490 views

How does the creative process of Biological Evolution work?

In order for nature to go from microbes to man, there needs to be new information. Where does this new information come from? It is understood that Evolution basically involves three elements. ...
1
vote
2answers
41 views

Why do we find nature 'beautiful', from an evolutionary perspective?

Most people I know find nature beautiful and holidays generally involve a place that has 'natural beauty'.
3
votes
1answer
30 views

Do people who have been blind all their lives dream?

Do the people who have been blind all their lives dream and do they experience or report "imagery" in the dreams?
-1
votes
1answer
42 views

Bilayer synthesis? [closed]

If we want to design a bilayer from Myristic acid (14 carbon fatty acid). The average bond length between C-C is 1.5 A. What will be average thickness of the membrane? Edited to include the OP ...
1
vote
1answer
29 views

Consequence of touching Formaldehyde

I accidently touched formaldehyde some days ago. Skin on my hand got dehydrated as if I had placed it in salt solution. I washed it with water and it returned to normal state after 5 minutes. But now ...
1
vote
1answer
21 views

Concentration of DNA by isopropanol

I have read that DNA can be concentrated by addition of isopropanol. What does "concentrated" mean? What does isopropanol do on a molecular level to concentrate DNA?
1
vote
2answers
37 views

Functioning of EDTA

I know that EDTA chelates metal ions. It weakens bacterial cell wall and inactivates the DNases. What is the reason why it can do so ? I guess it can inactivate DNases by altering the ...
2
votes
1answer
42 views

Why does ear wax taste sour?

Why does ear wax taste sour? I am interested in both the physiochemical mechanisms and the evolutionary reasons behind the sour taste of earwax.
1
vote
3answers
42 views

What problems do invasive species cause ?

Why do we value species? What is so bad about invasive species? Why value biodiversity?
0
votes
1answer
42 views

Do chicks in eggs excrete?

I want to know if, as a baby chick grows inside the egg, does it excrete waste? If it does excrete waste, does the waste just sit inside the egg with the chick, until the chick cracks open the egg ...
1
vote
1answer
29 views

What happens when we dream?

When a human dreams does the brain activate the cones and rods in the eyes creating the images we see in dreams?
2
votes
1answer
16 views

How do B memory cells provide immunological memory? Do they differentiate into plasma cells?

My text book doesn't say anything about how B memory cells actually ensure a faster response the second time the antigen is encountered. My guess is that they differentiate into plasma cells which ...
0
votes
1answer
22 views

Fertilization of an egg by two sperm

What happens when two sperm fertilize an egg? This can be either naturally or by experiment.
1
vote
1answer
24 views

Mutated Sperm - What happens?

I saw on TV that not all sperm are the same. Some have mutations like 2 and 3 tails? There were other mutations as well. If one of these mutated sperm actually fertilized an egg, would the embryo ...
1
vote
1answer
16 views

Affect of the health of the male parent at the time of conceiving

Does it matter at all in determining the traits of a baby what the current health of the father is? For example, let's say I got diabetes at the age of 40 because of the poor lifestyle choices. Will ...
1
vote
1answer
38 views

What is the current theory of sexual orientations in Biology?

What is the current explanation of what causes the different sexual orientations in humans.

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