1
vote
1answer
36 views

Plasmid Expression Vector [closed]

If I am going to transform a plasmid into a bacterium, which will then be fed to a C. elegans, the expression vector needs to be bacterial correct? The other option is to have a worm expression ...
3
votes
2answers
246 views

How can some animals see ultraviolet or infrared light?

I know that some animals like birds, bees, and fish can see ultraviolet and infrared light. Whether it to detect flowers that bare nectar, or the urine trails of prey. But what I don't understand is ...
1
vote
1answer
38 views

ATP yield of fermentation: study to cite?

Everywhere I find the ATP yields of respiratory and fermentative metabolism of glycolysis. While that of oxidative phosphorilation I could find in Stryer to cite one study where this was addressed ...
3
votes
0answers
406 views

What's the technical difference between fainting (and being unconscious) and falling asleep (and sleeping)?

This is actually a popular question along google results but the answers usually sound like: sleep is voluntary (oh is it?) and healthy fainting is result of accident and is not normal I read ...
0
votes
1answer
93 views

Do individual neurons communicate with the origin of thoughts?

When mapping the different neural pathways in the brain, often pictures such as these are drawn: Or similar versions. Clearly these sketches draw the neural pathways as being a two-sided ...
1
vote
0answers
34 views

Wash adsorbed protein without destroying Biotin-Avidin-Mechanism

I am looking for reliable protocols to wash protein (e.g. Fibronectin) adsorbed onto glass surfaces. According to Protein immobilization literature, people usually use 1% SDS and some incubation time. ...
5
votes
1answer
62 views

Do saltwater fish need to drink? [duplicate]

In my biology class we got the question if saltwater fish need to drink. We need to use the term osmosis, but I cannot think of a valid answer and we were allowed to ask help.
0
votes
0answers
12 views

Easiest way to digitally store pathlab reports,blood reports and other reports

I'm engineering student, and working on cheap way to store medical records of a person. I want to know way to partially digitize variety of reports, by partial I mean that I would store photo of ...
1
vote
1answer
39 views

Spread of malaria from an infected person

If a person is infected with malaria, how can that person be a part of spreading malaria? As in, if a female anopheles mosquito sucks blood out of that infected individual, how is it possible that ...
0
votes
0answers
39 views

Is it possible to measure in vivo enzyme kinetics?

I was wondering is there way in which we can measure enzyme kinetics in vivo specifically for enzymes acting on lipid substrates (where substrate is restricted to 2D membrane as oppose to freely ...
4
votes
0answers
74 views

What is this spider, and what is this object that emerged from its rear end?

I was observing this spider (caught in a Petri dish) under my microscope, when I noticed that a small, round object emerged from its rear end. It appeared to be black and white in appearance. I am ...
4
votes
1answer
77 views

Bacteria resistance to natural antibiotics? [duplicate]

It is a known fact that bacteria develop immunity to antibiotics. Why then did not all bacteria develop immunity to natural antibiotics like penicillin in the fungi? It is for sure that fungi did not ...
5
votes
1answer
39 views

What is the name of this moth found in Virginia USA?

Found in Virginia, USA on Nov 1 2015, 10:34 AM.
2
votes
1answer
73 views

All-trans-retinal being converted back to 11-cis-retinal or vitamin A

There are two pathways all-trans-retinal can take after detaching from the scotopsin: (1) it can convert back to 11-cis-retinal, or (2) it can convert to all-trans-retinol (form of vitamin A), which ...
0
votes
0answers
33 views

How long does it take for a six month old kitten to decompose to bones?

I loved my 6 month old cat very much but she passed away just yesterday after a prolonged illness. She was buried a couple feet deep wrapped in a blanket that she loved very much in New England, which ...
0
votes
0answers
7 views

Is there sex specific recombination data from the HapMap project?

I am looking at the recombination data the HapMap project makes available. I noticed that despite the fact that recombination occurs more frequently in females, the only data releases available that I ...
1
vote
1answer
31 views

How is the enzyme glycogen synthase regulated?

How is the enzyme glycogen synthase regulated in regards to glycogen synthesis? I think I understand that phosphorylation decreases its activity (through glycogen synthase kinase?), but what role do ...
3
votes
1answer
140 views

What happens to blood after a person dies?

After a human dies, what happens to their blood? I know that it tends to accumulate in the lower areas of the body, whichever parts are closest to the ground, but I am wondering about coagulation. ...
3
votes
1answer
90 views

Do lunar rhythms have really impact on plant growth?

It is commonly known that lunar rhythms have influence on plant growth, and there are even lunar calendars to know when planting, pruning, transplanting, harvesting, etc. I know that the lunar cycles ...
1
vote
2answers
277 views

What is the difference between fixation and loss of alleles in a population?

When running a simulation in PopG, with parameters: Population size: 100 AA fitness: 1.0 Aa fitness: 1.0 aa fitness: 1.0 Mutation A to a: 1.0E-6 Mutation a to A: 1.0E-6 Migration rate between ...
11
votes
2answers
681 views

Is global life expectancy normally distributed?

I am trying to find out what the world distribution of life expectancy looks like.
3
votes
1answer
85 views

What is the shortest mRNA the ribosome can read to produce a peptide?

This question came as a comment on a previous question regarding non-ribosomal peptide synthesis, and why Glutathione cannot be synthesized by the ribosome. In general, Glutathione has a "gamma" ...
0
votes
0answers
14 views

Steppe peoples method for healing horses' wounds

There was a custom among nomadic steppe peoples like Huns or Mongols of putting pieces of saltened meat in wounds of their horses to make them heal faster. Did it work? If so, how? I suppose it has ...
2
votes
1answer
37 views

What is the mechanism of folic acid deficiency and neural tube defects?

I am having difficulty finding the mechanism by which folic acid reduces the risk of neural tube defects. I know that it does so, but what in particular actually occurs with folic acid deficiency to ...
1
vote
0answers
36 views

What evidence is there that common PCR DNA purification kits can isolate DNA from pathogens engulfed in macrophages?

I have previously asked a more general question about how PCR sequencing reaches all possible DNA targets here. I want to ask more specifically now: what evidence is there that common PCR DNA ...
2
votes
0answers
19 views

Limits of brain neuronal spatial mapping

It is being claimed that brain maps the world space in (roughly) 1-to-1 correspondance of a place to a neuron (link1, link2). My question is: as the mapping happens in 1-to-1 manner, then is it ...
7
votes
1answer
190 views

Why and how is DNA synthesis so much faster then RNA synthesis?

DNA synthesis in E. coli is 20x faster than RNA synthesis at 1000nt/s vs 50nt/s. (Mirkin'05) I find that perplexing since DNA polymerization has better proofreading than the RNA variety, which ...
0
votes
1answer
21 views

Absorption of Topical Magnesium

Can Magnesium be absorbed through the skin? Some athletes believe that Homeopathic magnesium lotions can reduce muscle pain. I've tried this and it doesn't work nor can I see how it can.
3
votes
1answer
1k views

The mystery of coconut tree

Could anyone please explain to me, why the nail which is nailed in a coconut tree is not raised from ground level, when the tree is growing up?
4
votes
0answers
73 views

Why a particular species of bacteria give rise to particular type of colony? [closed]

Bacterial colony varies in form, elevation, margin, opacity, chromogenesis etc. What gives definite character to a colony and what is the source of all the diversity? Is the reason similar to that of ...
1
vote
1answer
47 views

Is there some definition of life which makes viruses undeterminable?

There are many different definitions of life (RNA, something that comes through evolution) but not one I have seen which could not determine wheter are viruses living things (even though there are ...
3
votes
0answers
27 views

How do I test if microbes have survived after dehydration?

I have a solution containing various bacteria and fungi. My aim is to place solution on filter paper, and wait until it dries. I then wish to test if the organisms have survived, either on the dried ...
1
vote
1answer
45 views

What are known methods of non-surgically disabling neural tracts/pathways (esp. Corpus Callosum)?

I think I've read something about usage of TTX and optogenetics, but I cannot find the papers for either anymore. Any reference would be much appreciated, especially those concerning mice and the ...
1
vote
0answers
23 views

How do furniture beetle larvae drink?

I have read that the furniture beetle (Anobium punctatum) has larva that will burrow for three to four years in wood before emerging and pupating. How do they obtain water during that time if they are ...
5
votes
2answers
2k views

Why do snails come out after the rain?

Many times, when there is rain after a long time, snails come out afterwards, and you can see snails and slugs everywhere! I have two questions: Why do they come out after the rain? Where are they ...
1
vote
2answers
58 views

Does common PCR amplify genes regardless of what cells / barriers they are in?

I have some understanding of how PCR testing works. What I have always been wondering: how can we be sure that a primer reacts with the targeted gene(s) regardless of where¹ the genes are inside a ...
3
votes
0answers
51 views

Relative densitometry from SDS PAGE

I'd like to perform densitometry on a Coomassie stained SDS PAGE gel to compare a recombinant protein's expression levels under two conditions. I'm using BioRad's Image Lab software. My questions are ...
3
votes
1answer
89 views

Needle-like crystals observed in wild-grape wine under microscope. Potentially harmful?

I recently crushed and strained about thirty pounds of wild grapes to ferment into wine. About a month in, I did a visual inspection of the wine under microscope to try and identify some yeast and ...
0
votes
0answers
20 views

Organ secretions

If an animal secrets an enzyme from an organ that is entirely dedicated for the same secretion, can one or cannot one just use the gene coding for that protein to obtain the protein in vitro. How much ...
1
vote
1answer
201 views

Problem on Probabilty of a restriction enzyme cutting a random DNA sequence

I think its a silly question to ask here. When I came to this site all I could see were the questions which asked detailed explanation behind a phenomenon and reasoning was there at first place. I am ...
0
votes
0answers
5 views

How much potential from heart reaches the chest

I learn about the ECG waveform of heart potential.How much voltage will actually reach our skin which is developed in the heart(from Sinoatrial node).What will be the approximate voltage range when it ...
1
vote
1answer
33 views

Conjugated deoxyribonucleotides

I'm currently learning about using PCR techniques to make fluorescently labelled DNA probes, and the textbook mentions "conjugated deoxyribonucleotides" Can someone explain what these are? Nothing ...
0
votes
0answers
12 views

Do you know of any scratch assay video databases?

It would be of great help for a statistical work we have been asked to carry out at our University. Thank you very much in advance.
2
votes
1answer
233 views

How do neurons inverse an action potential?

See the following figure (source): The pathway on the right (SN $\rightarrow$ interneuron $\rightarrow$ F Neuron $ \rightarrow $ flexor muscle), is explained as follows: The action potential in ...
7
votes
4answers
322 views

How does Darwinian Evolution work?

Let me explain... A friend and I read some articles, part of a Biology book, and watched a video on evolution. We then tried to explain what Evolution is to each other. My friend said that Natural ...
1
vote
1answer
112 views

Is a variable domain in immunoglobulin's heavy chain different from the one in light chain?

I guess yes, there is difference in amino acid sequences of $V_L$ and $V_H$. And so we have 6 different complementarity determining regions (CDRs) per monomeric immunoglobulin as two heavy chains are ...
3
votes
1answer
33 views

Brandywine Maple?

I'm collecting and classifying leaves for biology. Teacher took us on a 'walk' to help. She let us pick and told us one leaf was a Brandywine Maple and another later on, a different leaf, was a Red ...
5
votes
2answers
256 views

Why does depolarisation by high intracellular K+ trigger calcium channels opening?

I have learnt that in pancreatic beta cells, glucose being metabolised in the cell causes a high ATP level, which triggers ATP-dependent potassium channels to close. This means that potassium can't ...
0
votes
0answers
36 views

Distribution of synaptic connections

What is the roughly the distribution of the various synaptic connections in the brain. Consider the following types: axoaxonic synapse between the axon of one neuron and the axon of another ...
3
votes
1answer
162 views

Differences between synaptic connections

Consider the following synaptic connections (from here): axodendritic - A term pertaining to an excitatory or inhibitory synaptic connection between the presynaptic axon of a transmitting neuron ...

15 30 50 per page