0
votes
0answers
30 views

How do you amplify a specific AFLP marker?

I'm trying to clone a specific AFLP marker out of Medicago truncatula. I know the selective bases I need for the end of the primers. My problem is that all the AFLP cloning kits I have found are ...
0
votes
0answers
55 views

Analytical skill: Identify possible predator from grotesque collection of desiccated animal carcasses

Not sure this may be the right site for the question. Background: A resident of central india, parts of the house & annexe are a century old. The house is surrounded by a near copse - may be ...
0
votes
1answer
60 views

How does a smoker's pleasure work?

I smoke. For some reason, I've always had this idea that the pleasure of smoking works more or less like this: from the lungs, the nicotine reaches the blood, with which it travels to the brain, and ...
0
votes
1answer
49 views

Physiology of skin and leather: why do salt borders occur?

I discussed a leather store about the white border aka salt border on leather shoes. They said that it is due to inner features of leather and you could try to add some fat/cream to push it back ...
-1
votes
1answer
57 views

Can a certain part of human dna can be replaced with other animals dna?

Human DNA consists of adenine, thymine, guanine and cytosine. Can thymine of other animal can be placed with humans thymine?
0
votes
1answer
54 views

Difficulty in developing certain vaccines

I have a college level background in Biology, say at the level of Campbell. I am very curious to know why it's extremely difficult to develop vaccines for certain diseases. Two cases which I am really ...
4
votes
3answers
398 views

Are humans the only animal that requires “clean water”?

I've seen a number of animals - dogs, cats, squirrels, ducks and geese, etc drink from puddles, some of them were muddy, others had green flora growing under water. Same goes for lakes and rivers. A ...
1
vote
0answers
30 views

Referencing the homologous chromosomes

There exist a co-ordinate system from chromosomes like "12p11.3". In this system, first integer range from 1 to 23 i.e it takes homologous chromosomes as a pair. If we want to distinguish among ...
4
votes
1answer
106 views

What constitutes the determination of a normal blood glucose level?

Several on-line resources (e.g. MedlinePlus) suggest: Normal range for non-diabetic people who are fasting should range between: 70 and 100 milligrams per deciliter (mg/dL) Non-diabetic people who ...
1
vote
1answer
592 views
3
votes
1answer
64 views

What's the use of RNA editing in kinetoplast?

The genes of the mitochondrion (kinetoplast) of some Kinetoplastida - e.g. Trypanosoma brucei, the parasite which causes African sleeping sickness - are "encoded" in a way that the pre mRNA requiries ...
1
vote
1answer
49 views

Why PPD test in patients without M.tuberculosis often slightly positive?

I got this question which I interpret as Why PPD test in patients without M. tuberculosis often have slightly positive PPD reaction i.e., a lifelong slightly positive skin test reaction? because ...
1
vote
3answers
73 views

Why HIV patients have greater virulence of M.tuberculosis?

I am reading Murray Microbiology book. Some facts M. tuberculosis is an intracellular pathogen. At the time of exposure, M. tuberculosis enters the respiratory airways and infectious particles ...
1
vote
1answer
72 views

Transcribing DNA to mRNA with introns

I have a problem in my bioinformatics class that I thought I was doing right, but someone else is getting a different answer. Here is the problem: Given the following DNA sequence, ...
0
votes
1answer
76 views

Some trophoblast excreting digestive enzymes in implantation

I found this paragraph in my study materials about implantation Original Simplest trophoblast excretes trypsin related substances and embedding consists of 3 stages: The blastocyst ...
4
votes
1answer
107 views

Why plants (eg. parsley) can keep vitamin C despite all the sun?

I have read that vitamin C is highly sensitive to light. So, how could parsley, for instance keep its vitamin C as it's flooded with sunlight?
-1
votes
1answer
53 views

What is the impact of prolonged electromagnetic exposure on human health?

What are the health effects of being exposed to a very strong electromagnetic field caused by two resonating superconductive coils (not static magnets) for a period of years?
1
vote
0answers
22 views

recombinant gametes

Suppose you are able to observe under a microscope the total number of meiotic divisions occurring in one gonad of a given individual and to outnumber exactly the crossovers between two given ...
2
votes
1answer
99 views

Transition/Transversion ratio

I'm taking a bioinformatics class and at this point we are just going over basic stuff about molecular biology. Half of the class is doing reading/research on our own, so there isn't much class time ...
2
votes
1answer
50 views

Reduction of size in diatoms due to asexual reproduction

From this we can see that diatoms reduce their size after asexual reproduction. This would ultimately lead to nonviable small size. Is there any mechanism to avoid this ?
2
votes
2answers
55 views

Connection between genes and pathways

I am reading about a paper about inferencing pathway information in cancer cells. Authors refer to ERBB2 as a gene and a pathway. I don't have solid biology background. What exactly means when we ...
3
votes
2answers
66 views

Detecting cancer or a genetic predisposition based on DNA sequencing

I am not by any means a biologist - so go easy. What would be a method for determining whether or not a patient has cancer based only on a genomic sequence? Update Thanks for the help in revising ...
1
vote
1answer
28 views

At what cancer stage do tumors release circulating tumor cells into the blood?

And would a very accurate sensitive system for detecting circulating tumor cells (which detects 1 cell per 50 billion) be useful as a screening tool ?
3
votes
1answer
57 views

Is there a database containing sequences of human cell lines?

I'm looking for the whole genome sequences of several human cell lines, e.g., A549 or Ea.hy.926. Is there a database specifically dedicated to human cell lines?
0
votes
1answer
44 views

Is “monosexual” still a valid term in biology?

I read an older article where they talk about species being strictly monosexual as opposite of being hermaphrodite, etc [1]. After an extensive googling and reading about how everybody has to be at ...
3
votes
2answers
121 views

Question about Autosomal Recessive Alleles

I had a homework question that I could not figure out. It states: A woman has cystic fibrosis in her family and did not want to have a child that suffered from the disease. She and her spouse ...
3
votes
2answers
72 views

Why are dolphins so smart?

It seems that dolphins have (among?) the highest encephalization quotients found outside of humans, higher even than chimpanzees. By the "use it or lose it" principle this indicates that intelligence ...
5
votes
1answer
146 views

What's the recommended maximum passage number for mammalian cells (NCI-H441)?

Is there a generally accepted maximum number of times you should split and reseed mammalian culture cells before restarting cultures from new stock?
1
vote
0answers
31 views

Pharmacodynamics of Ethinyl estradiol + Drospirenone and interactions with istotertinoin

Does pharmacodynamics of Ethinyl estradiol + Drospirenone in OCP pills like (Yaz, Yasminelle etc.) while using isotretinoin (Axotret, Roaccutane etc.) is changed? Does isotretinoin weakens the OCP ...
3
votes
1answer
402 views

Classification of glycosidic anomeric bonds (alpha vs. beta)

In the process of studying for my upcoming biochemistry exam, I have stumbled over the classification of glycosidic bonds. I want to be able to distinguish $\alpha$- from $\beta$-glycosidic bonds. ...
1
vote
1answer
52 views

Botany related question: liverwort sporophyte

Is perianth part of the sporophyte or gametophyte in liverworts (Marchantiophyta)?
1
vote
1answer
121 views

Determining a patients biological age?

I am looking for an algorithm that shows the exact coefficients some variables that have on a hospital patient's chronological age versus their biological age. I understand a lot of the variables ...
-2
votes
2answers
78 views

Could a normative population ever be non-heterosexual? [closed]

In the modern documentary called "Bi the way", one American geneticist said that if modern American youngsters may as well be functionally bisexual, then some generations ahead Americans could be ...
-1
votes
1answer
70 views

Can pheromones in perfume be formulated to cause agitation? [closed]

I generally don't care for female perfume. I have very sensitive olfactory receptors and in general, I just find perfume overwhelming. A couple weeks ago, my wife of almost 14 years and I were ...
1
vote
2answers
54 views

Removing a restriction site and introducing other at its place

What would you do if you want to remove an EcoRI restriction site and introduce BamHI restriction site at apprx. the same location ? One of the answers in my textbook was : To construct a DNA ...
2
votes
2answers
90 views

Is it possible to create an experiment in house conditions to prove the existence of germs?

I realise that the best way to prove the existence of germs or single-celled organisms is by looking at them through a microscope. But I don't have a microscope at my home, how can I design an ...
2
votes
0answers
40 views

Are there baby drinks closer to breast-milk?

Many women cannot or will not feed their children with breast-milk. It is my understanding breast-milk has many advantages over formula. Some psychological like oxytocin and some physiological like ...
2
votes
4answers
96 views

Does a phenotype include the presence of a recessive gene

I am trying to understand what exactly a phenotype is to try and understand a genetics database. (Ie I don't really know very much about genetics at all) I've been reading through wikipedia on what a ...
2
votes
1answer
74 views

What is the name of the smallest self-replicating thing?

Some time last year, I found an article on Wikipedia about the smallest something to be able to reproduce. I don't remember exactly what it was, but I am fairly certain that after the initial ...
2
votes
1answer
58 views

How good is information transfer between the hemispheres of the brain?

I sometimes listen to lectures on my phone using ear buds, but occasionally with only one ear bud in. Is the other half of my brain losing out on any aspect of the lecture? Just how good is the corpus ...
2
votes
1answer
42 views

maximum # of generations possible in a population of cannibalistic rats

Assume a sufficiently large population of rats (to allow genetic variation and facilitate inbreeding) in a place with abundant water, sunlight, shelter but no food. For how many generations can this ...
1
vote
1answer
31 views

Constitutive mutation in operator gene

If a constitutive mutation happens in the operator of an inducible operon, does that mean that repressors won't be able to bind them ? Or does it mean that even if repressors are bound, they will not ...
3
votes
0answers
64 views

Why don't surfactants destroy or incorporate into cell membranes?

How do surfactants remain on the surface of pneumocytes without (1) acting as a detergent or (2) the phospholipids getting incorporated into the membranes of pneumocytes... I'm guessing the answer ...
3
votes
1answer
171 views

For people who work in microbiology labs, what software do you use to manage your strains?

It's just a small microbiology lab that currently records everything on paper, and there's quite few mutants as well. Is Excel commonly used for this sort of thing? Or is there a better software to ...
0
votes
0answers
30 views

How to predict the position of the femoral head center given surface anthropometric data?

Given surface measurements such as hip breadth and hip depth, how would you predict the location of the femoral head center?
1
vote
0answers
59 views

DNA content in seeds vs. fruit flesh

Is there a publication comparing DNA yield and / or PCR-amplifiability after extraction from fruit flesh (like apples, oranges, cherries etc) in comparison to seeds of the same fruits ? I would prefer ...
1
vote
1answer
77 views

Insect identification

It is found in my apartment in Finland. I had to kill it to stop it from getting away before looking for the camera, but it looked pretty much the same alive and intact. It's length is about half a ...
2
votes
1answer
48 views

Evolutionary conservativeness

List the following proteins in the order of decreasing evolutionary conservativeness of their primary structure: Somatotropin. Catalytic subunit of a DNA – polymerase. Histone H1. ...
1
vote
0answers
14 views

Phylogentic Footprinting Project

I'm working on performing phylogentic footprinting to figure out how many TRE sequences there are in the TWIST1 gene. (Sequence motifs). I'm trying to use ENSEMBL to gather orthologues of species in ...
5
votes
2answers
554 views

Why do we assume that the first humans were dark-skinned?

According to the article Dark skin and blue eyes: How Europeans once looked: It is widely accepted that Man's oldest common forefather was dark skinned, and that people became more pale as they ...

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