2
votes
1answer
67 views

NMR spectroscopy detection methods for proteins

While studying, I've stumbled upon a table comparing the sensitivities of different detection methods in NMR spectroscopy: "Direct", "DEPT" and "Inverse", where "Inverse" seems to be the most ...
1
vote
1answer
31 views

How to identify the genes that distal enhancers pair?

I am writing a project proposal and I have to talk about this problem: how to identify the genes that distal enhancers pair? I am really new to this topic and I don't know what it is all about. I ...
3
votes
1answer
40 views

How do nicks in the DNA strand affect the success of Long Range PCR?

Long Range PCR using NEB Master Mix - Hot Start Taq was working fine for me (amplicon sizes of ~10kb) but stopped working all of a sudden. Is it possible that many freeze-thaw cycles on the DNA ...
-2
votes
1answer
53 views

What prevents this blue lobster from becoming dominant?

Like everyone else in the world I saw the blue crustacean from becoming dominant? I would think the blue camo would be an evolution advantage.
2
votes
0answers
24 views

What should be the distance between plant promoter and gene?

I am trying to clone a rice gene under a different endogenous rice promoter.I will be cloning the CDS of the rice gene.So I wanted to know what is the minimum or maximum distance I should put between ...
2
votes
1answer
216 views

Enzyme Assay, absorbance change to concentration change

I have a few issues with this question. An enzyme assay is performed and the kinetic data graphed. The y-intercept of a Lineweaver-Burk plot is 0.6 min/ΔA. The substrate being assayed has a molar ...
4
votes
2answers
648 views

What happens if a person has the right side of the body totally removed (including the brain)? [closed]

I guess humans could live without just the right lobe of the liver or the right brain. Could a person without the whole right side of body survive? The left brain controls the right side of the ...
0
votes
1answer
38 views

Global Acidification or Warming [closed]

Which poses the greater threat to ecology Global Ocean Acidification or Warming?
3
votes
1answer
65 views

Looking for protocol to produce a drosophila primary cell line

I'm looking for a general (and easy, this is going to be for an undergrad class) protocol to isolate and culture primary cells from fruit flies.
3
votes
1answer
98 views

Main mechanism of the evolution of sexual dimorphism

Sexual dimorphism is very common and (normally) requires the evolution of sex-specific genetic variation/regulation so the genetic correlation between the sexes can be <1. Is there consensus as to ...
0
votes
1answer
66 views

How long does it take for commercial fertilizers to break down? [closed]

Sometimes we buy carrots that are abit bitter, it's the taste of fertilzer. Once i put a tomato in the fridge, and when i ate it, it was bitter like shweppes, and i figured that was fertilizer also. ...
1
vote
1answer
77 views

Calories burn rate calculation — scientific studies

There are many online calculators for measuring walking/running/bicycling calories burn rate. They differ in input parameters like some might need my weight as well as height and some don't. Almost ...
1
vote
2answers
29 views

Deduce length order of 4 decapeptides based on the Ramachandran plot

I have stumbled upon the following past exam question: 4 decapeptides A, B, C, D, made only of glycine residues, have different conformations, and the phi and psi angles of individual peptides ...
9
votes
2answers
95 views

Is mammalian vision processed as a sequence of frames?

I often read that people believe that human vision has an inherent frames-per-second rate (FPS) that causes stroboscopic effects - such as seeing the spokes of a rotating wheel apparently rotating at ...
3
votes
3answers
74 views

Western Blot: Fischer ECL substrate 2:1 instead of 1:1?

i recently developed a b-actin-ab-incubated PVDF-WB-Membrane with the Thermo Scientific Pierce ECL Kit. According to the manufacterers orders i mixed Reagent 1 and Reagent 2 1:1. But unfortunately i ...
1
vote
2answers
74 views

what is the modern, molecular explanation of independent assortment of alleles?

in case it isn't obvious i'll begin by stating that i'm a layman, trying to understand something about this subject from reading books. the books i've looked at so far give murky and confusing ...
0
votes
2answers
64 views

Rhizosphere vs. Endorhiza?

In microbiology and the naming of the various areas of the plant as it relates to microbial inhabitance, I am confused as to the difference between the terms endorhiza and rhizosphere. In this case I ...
1
vote
2answers
31 views

Does bioluminescence communcation occur on macroscopic level?

Some bacteria are known to communicate using bioluminescence (quorum sensing). Do we observe the same phenomena in any macroscopic species?
0
votes
5answers
139 views

How much does a typical biologist know about signal processing, machine learning, and (theoretical) computer science? [closed]

How much does a typical biologist know about signal processing, machine learning, and (theoretical) computer science? Coming from a neuroscience background where knowing about algorithms and computer ...
2
votes
2answers
40 views

How much are phase-contrast and dark-field microscopy used in biological research? [closed]

How much are phase-contrast and dark-field microscopy used in biological research today? It seems these were invented a long time ago, so I am thinking would it be a good idea to learn these ...
1
vote
1answer
57 views

How to measure the total number of cells in an average human body?

I have got one assignment to calculate the approximate sum total of all cells in the human body. How to tackle this problem? I know that the current statistics is $10^{13}$ cells. I wanted some hints. ...
2
votes
0answers
29 views

Subtypes of Acute myeloid leukemia

I am a computer scientist with no biological background and working on analyzing lab results of patients with Acute myeloid leukemia. They have been tagged with following subtypes of AML: AML with ...
25
votes
2answers
2k views

Why was disease transfer to the Americas one-way?

It is well known that the European colonists brought many infectious diseases to the Americas, and that these had a deadly effect on the native populations, because they had no immunity to them. Were ...
1
vote
1answer
48 views

Applying drugs to brain via scalp (transdermal)

Many medications are intended only for the brain, but are taken orally. Hence <10% finds its intended target, while the remaining >90% is at best wasted, and at worst causes unwanted side-effects. ...
2
votes
1answer
114 views

How do I interpret this graph regarding introduced genes and virus-infected cells?

This graph appeared in a practice test for the MCAT. I am trying to interpret it, but it confuses me a bit. On the x-axis we have some introduced genes, and on the y-axis we have % of cells ...
2
votes
1answer
36 views

Sansevieria species specification needed

What are the botanical differences between the species S. parva and S. senegambica? Please highlight the main ones. I have 2 groups of plants. One with label "Sansevieria dooneri" (a synonym of S. ...
1
vote
1answer
76 views

Do alternative start codons code for methionine after transcription?

There is some literature which shows that all start codons code for methionine. However, in the standard genetic code, the alternative start codons clearly code for leucine. Does that mean these ...
3
votes
3answers
255 views

Why has grey hair evolved?

A vast majority of humans get at least some grey hair as they age. As far as I know this applies to both genders and all races. Presumably this means that at least some grey haired humans have ...
2
votes
2answers
58 views

Why isn't Bubonic Plague as virulent as it once was?

Although I am admittedly not anywhere near an expert on any topic, I still have a hard time understanding how Black Death and Bubonic plague are one in the same. Since plague still exists, with news ...
3
votes
1answer
1k views

What is the ratio of breathing rate to heart rate?

Breathing rate and heart rate are directly connected. This is because the heart is connected to the lungs via the pulmonary artery. I first thought that breathing rate would be half of the heart rate ...
7
votes
2answers
108 views

(How) can a pink grasshopper exist?

I saw this foto on Reddit recently: Is this possible? How can a grasshopper become like this? Is this just natural genetic mutation?
3
votes
1answer
50 views

multiple HIV infection in same T cell

I was wondering can multiple HIV virus infecting same T cell ? Coz in flu virus they have SA to cleave of those sialic acid residue preventing re-infection of the same cell by other viruses to ...
1
vote
1answer
70 views

Regarding the difference between cDNA library and RNA sequencing (Biochem. technique)

I was wondering why establishment of cDNA library required the step of reverse transcription (i.e. turning the sequence into DNA) why not directly using the extracted RNA for sequencing and converting ...
1
vote
1answer
89 views

How to estimate the DNA density in human sperm head? [closed]

I have got an estimate of sperm head volume from internet.Like consider it as a disk of order 4-5µm. Now I wanted to find the DNA density in the sperm head. How to find that?
1
vote
1answer
29 views

What triggers the release of gonadotrophin-releasing hormone with the onset of puberty?

I am interested to know the process of development of puberty. During puberty, when boys/girls reache 12-14 (puberty) years of their age, their hypothalamus releases gonadotropin releasing hormone ...
6
votes
1answer
352 views

Alfred Jost's rabbit experiment: how did he actually do it?

I was watching a Crash Course on Biology YouTube video by Hank Green: Great Glands - Your Endocrine System. Hank humorously describes a sex determination experiment by Alfred Jost as follows ...
3
votes
2answers
43 views

Is the water transpired by plants pure H₂O, or does it contain trace minerals?

I'm considering the idea of drinking water that I trap from what is transpired by trees. Interestingly, is this water a fairly (or maybe even extraordinarily) pure form of H₂O, a remarkably pure ...
1
vote
1answer
49 views

Why do pine stands exist?

I live in the midwestern United States. I cycle to work, sometimes on heavily wooded roads, and I can't help but notice that in most places within the surrounding deciduous forests Pine and evergreen ...
4
votes
1answer
243 views

What is this beetle?

During a pit stop on the road from North Carolina to New York (somewhere in Delaware) I found the following (dead) insect in the car park: As you can see from the quarter next to it (25 mm ...
1
vote
1answer
49 views

What is the name of this big plant with a purple trunk and green to purple berries? [duplicate]

I have photographed the following plant in our garden in Pittsburgh, PA. Do you know the name of the plant?
1
vote
1answer
53 views

How do Type I restriction enzymes work?

According to NCBI, type I REases have a specific target sequence but randomly make cuts at sites far from the target sequence. How does the restriction enzyme travel from the target sequence to the ...
5
votes
1answer
77 views

Is oxidative phosphorylation less efficient in gram positive bacteria?

Gram negative bacteria perform oxidative phosphorylation in their periplasmic regions, between the inner and outer membrane where a proton gradient is maintained and used by the ATP synthase to make ...
4
votes
1answer
133 views

Null distribution of Blomberg's K

I've noticed different functions in different R libraries have different ways of assessing significance for Blomberg's K. For instance, picante's phylosignal looks at a distribution of PIC variance ...
9
votes
1answer
181 views

When did CRISPR/Cas9 evolve and what is the likelihood that a superior system for live cell genome editing has already evolved on earth since then?

I've read that CRISPR/Cas9 is currently being implemented and tested for its ability to edit genomes in live cells, and that it is supplanting other genome editing tools in labs, such as TALENs and ...
5
votes
2answers
160 views
49
votes
11answers
11k views

Can humans survive without consuming life? [closed]

Some people say that it's awful that humans eat animals. They feel that it's barbaric, because you're killing life and then on top of that, you're eating it, and that you should eat vegetation ...
5
votes
1answer
139 views

What are the atmospheric requirements for large dinosaurs?

What are the atmospheric requirements for large dinosaurs? and are the atmospheric constituents for supporting large dinosaurs any different from the atmosphere today?
3
votes
3answers
62 views

sex limited genome transmission

In general, for dioecious species, a large portion of the genome passed from parents to offspring of both sexes - in mammals the X-chromosomes and autosomes are passed from a mother to both daughters ...
3
votes
1answer
174 views

What is a protective epitope?

What is a protective epitope? An epitope is basically a part of antigen. So does it mean that when the epitope combines with an antibody, it helps in the functioning of the antibody instead of going ...
2
votes
0answers
51 views

How can oxygen production during photosynthesis be measured? [duplicate]

I'm curious how people measure quantitatively photosynthetic rate in labs. I've seen many qualitative examples with direct observations of bubbles forming in water due to photosynthetic output of ...

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