3
votes
1answer
88 views

Antibody-antigen database

Is there a database where I can find an affinity estimate if I provide a given antibody and a given antigen sequence ? Input : antibody + antigen sequence Output : quantitative binding/affinity ...
3
votes
1answer
367 views

Why re-label the strand ends in 3' DNA labelling?

I have a problem with a molecular biology question; I don’t understand how DNA 3’ labelling works. I took a diagram from my lesson and tried to understand with it; this is what I understood. If I’m ...
3
votes
1answer
21 views

How long does it take for the Opioids listed in the Description to induce Analgesia when Administered via IV?

How long does it take for the Opioids listed in the Description to induce Analgesia when Administered via IV? Now I don't mean how long it takes for euphoria to come on but analgesia. The opioids I ...
3
votes
1answer
108 views

How to wash the column during protein purification with GST tag?

I have been working with GST tagged proteins for the last 4 years and after loading the cell lysate into the column I was washing it with 20-30 column volumes of PBS and sometimes my proteins were ...
3
votes
1answer
100 views

Do serotonergic (5-HT2A-mediated) psychedelics elicit stimulant effects?

I know that 5-HT2A partial agonism is the key mechanism of action of serotonergic psychedelics but I also know that some research (e.g. the selective (no activity on any other known receptor sites) ...
3
votes
1answer
139 views

Which Receptors are Involved in the antidepressant effects of SSRIs?

From what I've read the major receptor subtypes involved in the antidepressant effects of SSRIs are: 5-HT1A 5-HT2C 5-HT3 5-HT6 Please cite journal articles to back up your claims, I don't want any ...
3
votes
1answer
44 views

How does Serotonergic (5-HT2A mediated) Psychedelia work?

How does serotonergic (5-HT2A specific) psychedelia work? I've read that there are some theories that it might involve the induction of a glutamate release in certain regions of the brain involved in ...
3
votes
2answers
1k views

Do neurons secrete multiple neurotransmitters, or just one type?

I know that neurons communicate between each other by filling the junction between dendrites with neurotransmitters. What interests me is if a single neuron only works with one type of ...
3
votes
1answer
103 views

Where does all the food go?

Food goes in, excrements come out. This seems obvious. But it seems like the mass of the excrements is less than the one of the food. Most people I asked answered: "There's a difference because it's ...
3
votes
1answer
78 views

Regeneration capabilities in humans?

To what extent can humans, (mammals in general), theoretically regenerate, and is their a way to speed up and/or exceed this original extent.
3
votes
1answer
35 views

Lateral, axional and temporal resolutions for imaging in vivo

What are the spatial (lateral and axional), and temporal resolutions that one is expected to get with the following optical imaging techniques? Optical Bioluminiscence Imaging (BLI) Optical ...
3
votes
3answers
1k views

Why does the apex of the human heart usually point to the left?

In the majority of human beings, the apex of the heart (left ventricle) points towards the left side of the body. Sometimes however (approx. 1/12000 births), a person is born with a condition known ...
3
votes
1answer
249 views

Are there records of monozygotic twins in which one experiences androgen insensitivity syndrome

I have given my high school biology students the thought experiment of "What would happen if a researcher induced twinning of a female zygote and then replaced one of the X Chromosomes with a copy of ...
3
votes
2answers
546 views

Physiological indicators of happiness and well being

If I understand it correctly, levels of serotonin in the brain can indicate if a person is happy. What other physiological measures indicate happiness or well being for a humans? I am looking for ...
3
votes
1answer
105 views

High protein turnover rate and protease inhibitors?

I work with mice, and I want to see what happens to some specific proteins in the mouse brain after IL-1b injection (intracerebroventricular). I have a problem: when I measure the mRNA and protein ...
3
votes
1answer
102 views

What to look for when buying a light microscope?

I am a science lover and want to buy a microscope to explore things around me. Like studying cell structures, microorganisms, blood and plants. It would be great if there is way to photograph slides. ...
3
votes
2answers
1k views

What is the inlet/outlet speed of air going in/out of our mouth during inhalation/exhalation

During inhalation, your alveoli expand, creating a pressure difference between the atmosphereic pressure and our lung sacks and therefore air will flow into the repspiratory airways. I am trying to ...
3
votes
1answer
109 views

What is the length of anaerobic biodigestion stages?

I have built an experimental portable batch biodigester to process organic garbage. A 50L drum filled half with garbage, half with water and with some cow dung added for inoculation, is connected from ...
3
votes
1answer
94 views

Cell growth conditions for preparing electrocompetent cells

Usually the protocol for preparing electrocompetent E. coli cells calls for growing the cells at 37deg and 225rpms until they reach OD of 0.3. I was wondering, is there any reason they should grow at ...
3
votes
1answer
47 views

mutant down but not out

I am interested in a gene which is null lethal but I need to temporary induce diminished capacity. If a cell is homozygous is it possible to induce heterozygous phenotypes or a partial knockout from ...
3
votes
2answers
198 views

Does the speed of electrical impulses through neurones decrease with age?

From what I read on the NatGeo app, it stated that the speed of the electrical impulses that are sent by a neurone will be approximately 332 kilometers per hour. Will the speed of this electrical ...
3
votes
1answer
264 views

Can Naegleria fowleri enter through the eyes ( example rinsing/splashing eyes with water)

I understand that it primarily enters the body through the nose by by "feeding on the olfactory neurons in the nose" as answered here in a different question. Would the nerve cells in the eyes present ...
3
votes
1answer
57 views

Brain response frequencies while sleeping

I'm not sure if this is the right place to ask this but Biology seemed to fit best. I'm trying to find out what frequencies the brain responds to best while sleeping. The reason for this is recently ...
3
votes
2answers
145 views

Why is most tissue cellular?

Most tissue is comprised of cells. Why? It would seem inefficient to have so many individual nucleus, membranes, etc.? Specifically: Not all tissue is cellular. Much tissue is extracellular matrix. ...
3
votes
1answer
105 views

Optimization of annealing temperature

I am trying to optimize annealing temperature for some primer pairs. I have tried optimization using cDNA, genomic DNA, Taq polymerase, phusion polymerase etc., but every time I am either getting ...
3
votes
2answers
126 views

What is the biological basis for a human's ability to think clearly and be aware while “fighting/competing”?

I'm interested in learning more about the biological systems or hormones or parts of the brain that affect short term (<15 minutes) ability to concentrate attention on the task and be aware of the ...
3
votes
1answer
48 views

Is there such thing as “biological cycles” of activity/performance/etc in mammals?

I'm looking for some information about the cycles of increased/decreased activity, mental performance or endurance that are related to a mammal's age and the time of a season. First of all, I'm not ...
3
votes
2answers
218 views

Short-term Lamarckism in asexual single cell organisms

I was reading through the Karr et al. (2012) whole-cell computational model. One of the things they did was to induce single-gene disruptions in their model. They observed several to be fatal, but: ...
3
votes
1answer
56 views

What are treatable foetal conditions during pregnancy?

What are different foetal conditions during pregnancy that could be treated with appropriate prenatal genetic testing? I only know that pregnant women are encouraged to take certain supplements to ...
3
votes
1answer
432 views

Is collagen supplementation useless?

When collagen is digested is it broken up into usable components that the body can use to produce its own collagen? What evidence is there that supplementing with collagen type I & II etc.. can ...
3
votes
1answer
495 views

Open protocol for Ligase Independent Cloning

Ligase Independent Cloning is a protocol that allow an insert to be integrated into a vector without ligation. It uses T4 DNA polymerase with only ATP to first chew back from blunt ends to create ...
3
votes
1answer
169 views

What is the DNA/protein charge ratio?

To study DNA-protein interaction, I want to do a DNA retardation test by mixing the protein with DNA and afterwarts loading it on an agarose gel to see if the DNA migrates slower. I've found some ...
3
votes
1answer
98 views

Cucumber on the eyes

In many spas and salons I have seen people place a cold cucumber on their eyes. I think this might be for coolness, but why a cucumber? And Is it actually for coolness or for something else (like ...
3
votes
1answer
209 views

Predicting progeny of recessive mutations using recombination

I was asked this question on a test and got it wrong, but I'd like to know how to do it. The answers are shown in the blanks below: You are studying two recessive mutations in the fruit fly D. ...
3
votes
1answer
906 views

Is secondary follicle or Graafian follicle arrested in the second metaphase of oogenesis?

My note about which I am not sure: Secondary oocyte is arrested in the metaphase II monthly after puberty until fertilization with sperm. The following picture also satisfies my sentence: . So ...
3
votes
1answer
257 views

Where does meiosis II of oogenesis end exactly in tuba uterina - uterus?

I know that metaphase II ends at ampulla tuba uterina, but I am not completely sure where the telophase II ends. Is it in the triangular section of cervix uterii? I just have an intuition that the ...
3
votes
1answer
182 views

How do you see the stage of the second meiotic arrest in oogenesis in the given video?

My old question raised this new question. After reading this page I can say now that metaphase is the stage in which the second meiotic arrest occurs within oogenesis: The oocyte is arrested again ...
3
votes
0answers
13 views

Scale up of Bacterial growth from 250mL to 1-5L?

I am currently growing up a specific strain of E.Coli with a knockout in 40mL of growth medium (LB) in a 250mL shaker flask... My ultimate goal is to scale up this process to a 1-5L large scale setup ...
3
votes
0answers
13 views

Why are particulate matter toxic to humans?

There are many studies on fine particles (PM2.5) and their fully negative effect on human health. There doesn't seem to have any positive aspect of inhaling particles, except very particular ones, but ...
3
votes
0answers
22 views

Hypersensitivity Simultaneously in Ear canals

I got experimental data where hypersensitivity (i.e. tonic pain when touching ear canal) was recognised for about 100 minutes. I do not know which type of hypersensitivity is this one. IgM can ...
3
votes
0answers
18 views

Does a presence of ants on a plant mean that the plant has been infested by aphids?

Ants protect aphids from their predators (such as ladybirds): Aphids and other hemipteran insects secrete a sweet liquid called honeydew, when they feed on plant sap. The sugars in honeydew are a ...
3
votes
0answers
19 views

Cross section of actin network in neurites

for a simulation I am developing I would like to know how the actin network in neurites is distributed. Is actin confined to the periphery or is the whole neurite shaft containing actin with a rather ...
3
votes
0answers
11 views

How to make sure a stem cell culture is cancer free?

Say I take a blood sample from an adult, extract the white cells and apply to them the process described in <this article>. Assuming this actually turns the adult white cells into stem cells, which ...
3
votes
0answers
82 views

Why doesn't honey spoil quickly? [duplicate]

Why is it that honey can last for decades and not spoil like other foods? Is there any chemical in honey which gives rise to this amazing feat?
3
votes
0answers
18 views

To measure Na/K channel activation in membrane level?

Assume that there are two different signals going on in the ECG - during depolarisation and repolarisation in the standard ECG. I got an advice that do not use the standard ECG in measuring exactly ...
3
votes
0answers
41 views

Intuitive explanation for Kin- and Group- selection

It is known from theoretician in the field of kin selection that kin selection (inclusive fitness theory) and group selection are actually two sides of the same coin. In other words, these two ...
3
votes
0answers
25 views

DNA barcoding, querry regarding tree based (cluster) methods for species identification

I am working on success rate of DNA barcoding in identification of species using distance and tree based methods. Regarding distance based method, I have used Adhoc and species identifier and ...
3
votes
0answers
45 views

Why does pasta really boil over? [migrated]

I was making pasta, and I noticed the pasta boiling over. I thought about it some more, and I realized I had no idea why this was happening. When the lid is on, the foam rises. When the lid is off, ...
3
votes
0answers
48 views

Accuracy of genome size estimation by flow cytometry

I'm working on a genome project and using an in silico k-mer analysis to estimate the size of our genome based on the available Illumina reads. The k-mer based estimate is consistent across a wide ...
3
votes
1answer
41 views

how can a pseudo coelomate like ascaris lay 200k eggs in a single day

as per this site the dimensions of eggs of ascaris lumbricoides are 78-105 microns and as it is shown in the pic down here only half of the body cavity would be used for storage of all the stages of ...

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