5
votes
2answers
217 views

The meaning of RNA-seq data

many papers I read mentioned "RNA-seq data". While searching for the meaning of this word, I could not find any layman's definition. As far as I understand, RNA-seq data is the complete RNA ...
5
votes
1answer
6k views

Why does high pH result in the denaturation of DNA?

In the Southern blot method, for example, a solution of NaOH is used to denature the DNA in the sample. I find this counterintuitive since I expected that $\text{Na}^+$ cations would neutralize the ...
5
votes
3answers
1k views

How does vasoconstriction/vasodilation change blood pressure?

Background: I am coming at this question from an electrical engineering background, and I feel like I am missing certain assumptions that are going into the statement found in my physiology textbook, ...
5
votes
1answer
919 views

Besides hemoglobin, what proteins are present in red blood cells?

I knew that mature red blood cells (RBCs) lacked nuclei, but I wasn't aware until just now that they also lacked ribosomes and mitochondria. Most cells in the human body all contain a common laundry ...
5
votes
1answer
124 views

What is an operon?

What is an operon in a eukaryotic cell, and how does it regulate the expression of genes? I've already read Wikipedia, but it is not enough clear to me. Unfortunately my knowledge in genetics are very ...
5
votes
5answers
97 views

Can an influenza virus carry other infectors with it?

This idea came to my mind when thinking about how many people become ill in many locations with similar symptoms (fever and rough cough) from Influenza. There are of course different types of ...
5
votes
1answer
1k views

Like other animals, why humans don't have species & breeds?

Like other animals, why don't humans have species & breeds ? Even my pet dog is one type of breed, but we (humans) lack breeds & species? What is the main reason?
5
votes
2answers
2k views

Why is the DNA codon table “equal” to the RNA codon table

Before anything else please pay attention of the double quotes on the "equal" in the title - I know they are not equal, but you will understand in a bit. If I look at the DNA codon table here or in ...
5
votes
2answers
592 views

How do you visualize RNA on a gel?

I have run an in vitro transcription reaction and produced some RNA of a single species and definite size. I would like to visualize it to check if the reaction worked. Can I do this on an agarose ...
5
votes
2answers
4k views

Why doesn't the human skin grow back exactly how it was before being damaged?

Very simple: Why (and how) is regenerated skin different from original ? As we know we lose skin cells that becomes the dust in out homes and it always grows back to full thickness right ? So when I ...
5
votes
2answers
307 views

How many different protein coding genes are in the Human Biome?

There are approximately 20k protein-coding genes found in the human genome. This number is presumably very small when considering all the genomes found in the diverse microbes associated with the ...
5
votes
1answer
4k views

What is the definition of a stringent/relaxed plasmid?

I have found a publication which proposes some definitions, including a definition for strict and relaxed replication. The definitions are: Relaxed control of plasmid replication. Relaxed control ...
5
votes
2answers
82 views

Standard Classification of Disease

I am working on a project for health center. It involves the creation of a database of all diseases. Currently I want to classify disease on the base of their category based on international standard. ...
5
votes
3answers
224 views

How easy is it to carry out de novo sequence assembly?

Today a colleague of mine asked the following question: " Assuming I need to build from 0, a chromosome of a fish, with short reads but no other reference whatsoever [de novo assembly]: ...
5
votes
2answers
3k views

Are Asians more genetically homogenous than other races?

I've heard that Asians (I'm not entirely sure which subgroup was being referred to) tend to be more genetically homogenous than other races, with people of African lineage being on the other end of ...
5
votes
2answers
15k views

How does extracellular potassium ion concentration and calcium ion concentration affect the excitability of a cell?

When extracellular $K^+$ concentration increase by a certain amount, excitability of cells is higher because the resting potential shifts toward a higher equilibrium potential of $K^+$, therefore ...
5
votes
2answers
546 views

Do we have enough diversity of antibodies to overcome almost any antigen?

I always wonder about the capability of diversity of our antibodies. Do we have enough antibodies to fit to every kind of molecule? Wont't it be easier if our antibodies are like clay and can be ...
5
votes
3answers
288 views

Can extinct animals be cloned?

Scientists have found mammoth blood, and are planning to clone a mammoth. How does one go from having its blood to a full blown living mammoth? Is it possible? Why does it matter if the blood is ...
5
votes
2answers
5k views

What is the function of nodes of Ranvier in axons?

In a neuroscience class I'm taking, it was explained that myelin covers axons in sections, the uncovered sections are called nodes of Ranvier, and signals propagate much faster in the covered ...
5
votes
3answers
246 views

What are the limitations of commercial-grade DNA genotyping compared to full sequencing?

I've heard about services like 23andme, which offer genetic testing to the general public. As a person who knows very little about genetics, I'm interested in the subject and would like to know what ...
5
votes
2answers
3k views

Do octopuses have better eyes than humans?

I've read that unlike humans, octopuses have eyes "designed" the "right way", i.e. with the nerve fibers behind the retina, thus getting rid of the blind spot we humans have as well as theoretically ...
5
votes
4answers
3k views

Why Do Ruminants Require A Multi-Compartment Stomach To Digest Food?

Cows, camels, sheep, goats, etc being ruminants must chew their food repeatedly by regurgitating their food from their first stomach compartment and chewing their 'cud'. This then finer chewed ...
5
votes
1answer
1k views

If proteins have an overall charge, how do membrane proteins traverse through the hydrophobic region of the plasma membrane?

These two concepts seem almost contradictory, proteins have a net negative charge due to the amino acids in them each having a small negative charge, yet membrane proteins are able to exist traversing ...
5
votes
1answer
172 views

Free-flowing cells and those that are stuck together?

I've been thinking about the development of an embryo from the zygote stage. How is it that when cytokinesis takes place at that stage, the cells all stick together in a little ball, but later in ...
5
votes
2answers
326 views

Energy use by muscles, actual work done by muscles and more

Lately, I've started exercising in the gym and outside. I've also started to look at the details of food I eat. Food usually has a label saying the amount of energy is inside it. For example, some ...
5
votes
3answers
636 views

When to measure resting heart rate and blood pressure for following day-to-day trend?

I'd like monitor my resting heart rate and blood pressure to see how they are affected by regular cardiovascular training and other events in life. When should I take the measurements in order to ...
5
votes
2answers
573 views

What did pangolin scales evolve from?

Were they developed anew or a heritage from fish?
5
votes
1answer
2k views

How does the enzyme ATP Synthase use a proton concentration gradient to make ATP?

I understand what the enzyme ATP synthase does, but I'm not exactly sure how it does it. I've heard that it uses rotary catalysis, but how exactly does this work? How is the energy from the H+ ion ...
5
votes
2answers
325 views

When does oxidation destroy prions?

It seems like a no-brainer than oxidation, playing the, er, role it does in the universe, would destroy prions just like it destroys everything else. But when does it do that? I assume this has been ...
5
votes
1answer
90 views

Why is it always Sodium that we talk of in blood pressure?

Why is that we emphasize so much on sodium in blood pressure? I understand that it is a major extracellular ion, but, it also is tightly regulated. So when something is that tightly regulated, won't ...
5
votes
1answer
151 views

Why are mice with a single X chromosome and no Y chromosome males?

I was searching online and I read this article Mice can be male without Y chromosome and this is a part of it: The experiments demonstrate that there are multiple ways to make males, says Richard ...
5
votes
1answer
181 views

When did mouth pipetting stop becoming a way to handle liquids in a lab?

Almost all modern lab protocols have an addendum prohibiting pipetting by mouth, instead mandating that a Gilson pipette, a rubber pipette ball, or a serological Pipet-Aid be used. However, it was ...
5
votes
3answers
138 views

Why are sight and sound prerequisites for intelligence?

Edward O. Wilson, in The Diversity of Life wrote (emphasis mine): Ninety-nine percent of the animals find their way by chemical trails. […] Animals are masters of this chemical channel, ...
5
votes
3answers
90 views

Why is there a seeming dichotomy between mobility and photosynthesis?

At least among more complex organisms, I cannot think of any examples of highly mobile species (like animals) that also incorporate photosynthesis. Perhaps there are examples that I'm unaware of, but ...
5
votes
1answer
78 views

Identify the plant

Can anyone tell me which plant or flower is this. I have found it in Darjeeling, West Bengal, India.
5
votes
1answer
172 views

Do humans have skin cells that trigger oxytocin release in the brain?

I was reading an article by Montgomery on the therapeutic effect of pets (Boston Globe, January 12, 2015), and I found this quote: All animals appear to have cells directly under the skin that ...
5
votes
2answers
64 views

What ways are there to determine how big genera are?

I want to look at genera as a whole, across the animal kingdom to determine the range of sizes of genera. I have examined species richness and genetic diversity (pairwise distance from sequence data ...
5
votes
2answers
444 views

Why cannot there be multiple sources for same species origins?

We often associate Africa as the geographical location of the origin of humans. Why cannot there exist multiple geographic locations of origin (given same environmental conditions)? The same ...
5
votes
1answer
138 views

Is it possible to express a mutant gene only in a specific tissue?

Imagine that someone tries to develop a knockout mouse for a gene, but this result in lethality for the homozygous. Is it possible to express that mutant gene only in a specific tissue of interest to ...
5
votes
1answer
289 views

Biological origin of shuddering during sleep

What is the biological origin of the following behavior: Sometimes, during the early, formative phase of sleep (when you have a gradually dimming consciousness of what all is happening around), you ...
5
votes
3answers
185 views

Can we knock out Caspase-9 *and* avoid breast cancer phenotype in our mouse model?

I am trying to design a wet lab experiment with no wet lab experience to name. Right now, in my dream land, it would be excellent if it were possible to create a Caspase-9 knockout mouse (damage to ...
5
votes
1answer
2k views

What is our skin made up of?

Again, it is a basic question. What is our skin made up of? is it made up of many cells arranged in a systematic way or is it just like any layer say of a book?? what is the difference? where is the ...
5
votes
1answer
245 views

Plasmid in the nucleus and gene expression

If we insert a plasmid into a human nucleus that contains exact copy of gene and all relevant promoters to produce some human protein, will the cell create functional protein from that plasmid only ...
5
votes
1answer
67 views

How much of the weight of yoghurt is due to bacteria?

I recently started making all kinds of bacteria-processed foods by myself (such as yoghurt, but also Sauerteig and so on), and I found myself wondering how much of the weight of these products is ...
5
votes
2answers
2k views

Can the human eye distinguish colors in the periphery?

In the back of my mind I have the idea that human eyes can't notice the color of objects in the far periphery, and that any subjective perception of colors is done by the brain that tries to fill in ...
5
votes
1answer
4k views

What is the H+ gradient in mitochondria?

I would like to understand what the term H+ Gradient means. I googled this question and found terms such as chemiosmosis and ion gradient being tossed around. I am very new to biology and I do not ...
5
votes
1answer
944 views

Are fish and reptilian scales homologous?

Wikipedia: Fish scales are dermally derived, specifically in the mesoderm. This fact distinguishes them from reptile scales paleontologically. So aren't reptilia scales also dermally derived?
5
votes
1answer
196 views

Does gray/white hair turn gray/white from a normal hair? or does a gray/white hair actually grow gray/white originally?

I am really curious if hair actually tuns white/gray from default color, or does it have a defect as it is made and grows out of your head a different color?
5
votes
3answers
515 views

If someone were to die on the moon, would their body decay?

I heard that the footprints of Neil Armstrong are still there, so I was wondering if someone were to die there, would they remain preserved, too? If not how long would it take for them to decay?
5
votes
2answers
356 views

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