5
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1answer
62 views

What regulates these two methods of splicing?

Alternative splicing can shuffle the exon order and select from the exon sequence of a single gene site to provide a combinatorial selection of possible transcription products from the one gene. Can ...
5
votes
1answer
154 views

Do lunar rhythms have impact on human biology, if so, how?

I'm approaching this question from the circadian rhythm perspective. A circadian rhythm is a 24 hour cycle. While doing research on deep sea circadian rhythms, I found this abstract which seemed to ...
5
votes
1answer
394 views

Are there eukaryotes without introns?

This question on the function of introns in eukaryotic genes made me think: I know that more basal organisms have smaller introns and fewer alternatively spliced exons compared to mammals. But are ...
5
votes
1answer
252 views

Are descriptive statistics sometimes more useful than tests of significance?

How does the biology community currently feel with regards to publishing descriptive and effect size statistics rather than significance stats? Almost every journal article I read in the cell biology ...
5
votes
1answer
104 views

What controls leaf senescence in deciduous tree species, and how can I predict it?

If I want to predict litterfall, what data do I need to collect, and what statistical model might I use? For example, I might use the following coding to record phenological stage every other day, or ...
5
votes
1answer
109 views

Is 1 g/l living biomass for a Biogas fermenter plausible?

A usual number for a healthy biogas fermenter is $10^9 - 10^{10}/mL$ Bacteria, 10-15% of which would be methanogenic archae. Exceptionally healthy fermenters have more total bacteria and up 25% ...
5
votes
1answer
922 views

Is human face more resistant to cold than other body parts?

Walking outside tonight in -15˚C I realized an interesting observation: While I felt cold on most part of my body, my face (although the only uncovered part) didn't really feel cold. It registered the ...
5
votes
1answer
152 views

functional hypocholesterolemia due to very high HDL or low LDL?

Nutritional and medical recommendations about cholesterol seem to focus exclusively on hypercholesterolemia, and as such, they provide only a maximum desirable level for LDL and a minimum for HDL. ...
5
votes
3answers
401 views

What is the inbreeding coefficient for the female offspring of a sib-mating in a haplodiploid system?

The inbreeding coefficient of a sibling mating for a diploid organism is 0.25 (each allele has a 25% of being identical by descent in the offspring). In haplodiploid organisms, males result from ...
5
votes
1answer
626 views

Using ion-exchange chromatography to purify DNA from a cell extract - Is DNA more negatively charged then RNA?

When applying this method we have a glass or plastic column of resin which is positively charged. Then we pour cell extract into the column in order to capture the negatively charged particles which ...
5
votes
2answers
632 views

Sparking during electroporation of plasmid DNA into bacterial cells

During electroporation of bacterial cells (I work with Mycobacterium tuberculosis, but I think this applies to E. coli as well), sometimes I get sparking. I've read this is due to salts present, ...
5
votes
1answer
175 views

Why was polyploidy not lethal in certain octodontid rodents?

As discussed in Why is polyploidy lethal for some organisms while for others is not?, polyploidy is normally lethal in mammals. However, two species of Octodontidae (South American rodents), are ...
5
votes
3answers
60 views

Do you know of any disease where HLA / MHC association discovery has led to better treatment or management?

If we discover an association between a disease and a particular HLA type (MHC class I or class II molecule) then it may provide us with some insight into the disease in a very basic way. However, ...
5
votes
1answer
126 views

Is extreme cladism a mainstream position in the species debate?

In the philosophy of biology it has been claimed many times that a popular position regarding the question of what species are, among biologists, is cladism. For my current purposes, the defining ...
5
votes
1answer
125 views

What is the distance between the 3' 18s rRNA (the Kozak consensus sequence) and the A-site of eukaryotic ribosomes during protein translation?

Reposted from Quora The Kozak sequence is shown to be important in translation initiation, and the A-site is the anti-codon recognition site. If the distance is not known, how could I find out what ...
5
votes
3answers
2k views

Fetal development, gastrulation and embryonic disc

I am completely confused by the images circulating on the internet of human gastrulation. First, lets see how it happens in deuterostomes. This image depicts the process: (image is from Wikipedia) ...
5
votes
0answers
63 views

How to fix a small pond that has become stagnant? [migrated]

I'm working in a park and we have a small pond a part of a river system that has become stagnant, mucky and smelly. The river that previously ran into the pond is no longer there and we'd like to be ...
5
votes
1answer
83 views

Is it theoretically possible to restore the Tiger population to 100,000?

The current global Tiger population is roughly 5100 according to Wikipedia. With habitat destruction and poaching continuing and no effective improvement in conservation, most likely the Tiger ...
5
votes
0answers
68 views

What is basis of multifunctionality of “master glands” in the endocrine system?

I have just started reading about the endocrine system and I am having some difficulty understanding the basis of distribution of glands and associated hormones. I am using multifuntionality to ...
5
votes
0answers
67 views

Why do some medicines induce sleep? [closed]

I have seen lot of people sleep or say that they are feeling sleepy after taking some medicines. Why? I mean, if a medicine acts on some cell of the body because that cell has got its receptors and ...
5
votes
1answer
48 views

Is there a karyotype database for human cell lines?

I'm looking for the karyotype of a specific cell line. Some karyotype are well known, such as HeLa or some carcinomas, but some are very hard to find, such as LG2 (B cells). Is there a database ...
5
votes
0answers
43 views

How pronounced is the difference if we would change the running direction for runners?

I was jogging when I asked myself this question: In running contests the standard running direction is counterclockwise; I also believe to remember that the regulation is very old (right back to the ...
5
votes
1answer
132 views

How does the brain cool itself?

Thoughout life everyone tells you that brain is essentially a computer but just like computers your brain would create immense amounts of heat by being in use, so if that's the case how does it cool ...
5
votes
0answers
71 views

SDS Laemmli Gel that did not solidify [closed]

In my lab class this week my partner and I were making a SDS-PAGE Laemmli Gel. We made the resolving gel solution which was made of 30% Acrylamide, DI water, 1.5 M Tris-HCL pH8.8, and 20% SDS. We ...
5
votes
0answers
41 views

Do blood glucose “spikes” contribute to insulin resistance, or is prolonged elevation required?

There's increasing public discussion about the health risks of chronic insulin resistance* (IR). In many cases there's a focus on high glycemic index foods that "spike" your blood sugar, which implies ...
5
votes
0answers
88 views

Contact Inhibition of Cell Division: Signaling Pathway

The following article refers to contact inhibition of cell division in epithelial cells, specifically MDCK cells: Collective and single cell behavior in epithelial contact inhibition. In their review ...
5
votes
0answers
54 views

What were the first neural systems like?

I'm curious about the origin of the neural network. I'm thinking perhaps once life evolved beyond the single cell organism, it needed a simple neural network to coordinate those cells, and cell ...
5
votes
0answers
57 views

Statistical method for characterizing the relationship between body mass and metabolic rate

There is a dataset that contains body mass ($x$) and metabolic rate ($y$) from many different organisms. It is common to fit the data to the model of the form $y=ax^b$ and estimate the parameters $a$ ...
5
votes
0answers
18 views

Would constant light or fading light be registered as more wakefulness promoting by the Supra Chiasmatic Nucleus?

During the fall and winter season, a number of people are experiencing Seasonal Affective Disorder. One of the way it has traditionally been treated is with a dedicated lightbox - a very high ...
5
votes
0answers
57 views

Can microdialysis be made in Drosophila melanogaster?

I've asked this before in stack overflow's cognitive science community, and someone recommended me to ask here: I've found a couple of studies using microdialysis on insects, but didn't found any in ...
5
votes
0answers
85 views

Where can I get numerical data for the PAR (photosynthetically active radiation) curve?

I'm trying to do some rough figuring to calculating efficiencies of various light sources for growing plants. I'm hampered, however, by the lack of a curve to multiply by the spectrum of light ...
5
votes
0answers
236 views

Genetic expression in interspecific hybrids

Referring to interspecific hybrids, I have the following two questions:- Quoting from wikipedia:- The offspring of an interspecific cross are very often sterile; thus, hybrid sterility ...
5
votes
0answers
55 views

Honeybee decision process: new food, new home, new threat [closed]

I have heard that honeybees send out scouts, then based on the scout reports more bees go then come back and report and so they are supposed to be efficient decision makers. Can you give as complete ...
5
votes
0answers
117 views

Comparative cost of RNA-seq vs sequencing full length cDNAs

I am in the process of assembling and annotating the genome of a non-model organism, using almost exclusively short read (paired-end Illumina) data. Throughput is one obvious benefit of these data ...
5
votes
0answers
79 views

effect of background selection on promoter regions compared to distant enhancers?

Has anyone looked at the effect of background selection on the levels of conservation of promoter regions compared to distant enhancers? Do promoter regions have a higher conservation due to ...
5
votes
0answers
47 views

Serological assays not detecting native proteins [closed]

Is there anyone out there who has done much work with serological assays? We have antiserum for a manufactured viral protein but no luck so far getting it to detect native protein (unless today's ...
5
votes
1answer
86 views

Why would we overexpress Sir2 by overexpressing its hypomorph (dSir2-EP2300) in C. elegans?

Can't we just overexpress regular Sir2 in the paper? Rather than overexpress a reduced-function gene? The paper is Burnett C, Valentini S, Cabreiro F, Goss M, Somogyvári M, Piper MD, Hoddinott M, ...
4
votes
2answers
2k views

What is the contribution of the cockroach to the ecological environment?

I've read a lot about cockroaches but was unable to find how they contribute to the ecological environment. Does anyone know?
4
votes
2answers
1k views

Is pork poisonous?

Besides religious prohibition, there are several non-religious arguments against eating pork. A few of which are: Pigs and swine are so poisonous that you can hardly kill them with strychnine or ...
4
votes
3answers
986 views

Why is wind cold for humans? [closed]

Coming out of the pool the other day, I felt very cold because I was wet and the wind picked up. My idea is that a "cold" feeling should be associated with low temperatures, but it's indeed wrong. ...
4
votes
5answers
588 views

What is a good miniprep protocol for the class room?

I'm trying to find a good protocol for plasmid minipreps and I'm looking at 3 preps I've found: Using phenol/chloroform extract with phenol:chloroform:isoamylalcohol, isopropanol precipitation, ...
4
votes
2answers
409 views

Why is the strength of genetic drift inversely proportional to the population size?

I saw a concept on the Internet that says "the strength of genetic drift is inversely proportional to the population size". I don't know why they are inversely proportional? Can somebody explain? ...
4
votes
2answers
803 views

If DNA has a half life of about 500 years, how can old seeds be planted?

According to this article, DNA has a half life of 512 years Wikipedia claims that there are seeds planted as old as 31000 years Doing the basic math $100 / (2^{30000/512})$ we get that only about ...
4
votes
4answers
1k views

Do male animals visually perceive females as more/less attractive?

If we somehow remove pheromones, do animals experience a phenomenon similar to human "visual beauty" when looking at members of the opposite sex? For example, given a set of 20 female ducks observed ...
4
votes
3answers
10k views

Evolutionarily speaking, why do humans have 46 chromosomes

In humans, each cell normally contains 23 pairs of chromosomes, for a total of 46. Monkeys, chimpanzees, and Apes have 24 pairs (twenty-four pairs), for a total of 48. What caused humans to have 46? ...
4
votes
3answers
174 views

Gametes of two different species

I always wondered why gametes from two different species dont fuse together to form an offspring. eg a donkey (sperm) and a female dog (egg) I know this is not possible but I'm just curious.
4
votes
3answers
3k views

What if rB=C in hamilton's rule?

Hamilton's rule states that if rB>C then a gene giving altruistic behaviour will increase in frequency in the population. What would happen if rB=C? Will an individual perform the altruistic act?
4
votes
3answers
427 views

How many different kinds of polypeptides, each composed of 12 amino acids, could be synthesized using the 20 common amino acids?

How many different kinds of polypeptides, each composed of 12 amino acids, could be synthesized using the 20 common amino acids? The book's answer is $20^{12}$. However, I disagree. This result ...
4
votes
4answers
299 views

Biodiversity is restricted by genome combinatorics?

Me and some friends are interested in opinions for the following: Conjecture The maximum number of species must be limited by the maximum combinatorial/permutational space that can be occupied ...
4
votes
2answers
1k views

Is there an organism which has a common English name which overlaps with another organism's scientific name (or vice versa?)

Many organisms have their common names identical to their scientific names. For example, there exist apes of the genus Gorilla known commonly as gorillas, and plants of the genus Delphinium known as ...

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