4
votes
1answer
25 views

How to make sure a stem cell culture is cancer free?

Say I take a blood sample from an adult, extract the white cells and apply to them the process described in <this article>. Assuming this actually turns the adult white cells into stem cells, which ...
5
votes
1answer
120 views

How does the cornea breathe during sleep?

The cornea is not supplied with blood vessels and so exchanges gases directly with the atmosphere. So how does it breathe during sleep when our eyes are closed?
0
votes
1answer
34 views

Is “Cisternal Twins” an actual classification of twin types and if so, describe it

I always assumed my brother and I were identical twins. Now my mother, in her eighties, says we are cisternal twins, but I cannot find any references on the matter. Is there such a thing? If so, what ...
9
votes
3answers
508 views

Do memories have mass?

If it were possible to live forever, would our brains grow infinitely with the number of memories that we store? Or would we remove old memories as we create new ones?
4
votes
1answer
124 views

Why should plants transform glucose into sucrose before transporting it to other parts?

I've learned that plants transform glucose into sucrose before sending it into phloem. But the process seems to be complex and energy comsuming. Why should plants do it? Is it really necessary?
2
votes
1answer
68 views

Carrying or packaging capacity of SAD B19 dG rabies virus

I'm wondering about the carrying capacity also referred to packaging capacity or loading capacity (how many base pairs can be packed efficiently into virions) of the pseudotyped rabies virus SAD-dG as ...
1
vote
0answers
35 views

Glutamine analysis

I use to run a method to analyse all the amino-acids in a food sample. For that I have to hydrololyse the sample and in the last stage of the method I read the amino acids with a ion exchange ...
1
vote
0answers
19 views

Which classes of drugs (filed by their pharmacology) induce a release of β-endorphin?

Which classes of drugs (filed by their binding sites) induce a release of β-endorphin? I know of agonists of the nAChRs and 5-HT1A and ethanol. Are there any others? Please cite journal articles ...
-1
votes
1answer
32 views

What is phenotyping of blood during transfusion process?

Thallaesemic patients are advised to have only "phenotypically matched blood".I mean the transfusion problems arise mainly due to "grouping problems" like mismatch b/w ABO & Rh+/- so what do this ...
2
votes
1answer
29 views

Does a bird know it's different? [closed]

I live in Northern Virginia, USA. As I am sitting here there is a large flock of small (blackish) birds in a tree. They are quite prevalent here. For the sake of brevity I will call these birds ...
0
votes
1answer
13 views

Rosetta strain with chaperones for protein expression?

I am trying to purify a protein, and I was wondering if it is reasonably straightforward to obtain E.coli cells containing: -pGroe plasmids expressing chaperones. -Rosetta plasmids with codons that ...
0
votes
1answer
19 views

Which three functional groups are found in the general structure of amino acids? [closed]

Yes, I know, not two, but THREE. EDIT: Yes, sorry but I did quite a bit of research, searching my notes and textbook for the answer, but all I could find was TWO functional groups as a opposed to the ...
0
votes
0answers
19 views

How to check HCV genomic sequences between Two samples is different or not? [on hold]

I am looking to determine if the Hepatitis C Virus genomic sequences (variant population structure) between two samples is different to a statistically significant degree. If the nucleotide ...
1
vote
1answer
13 views

how to re-sequence a few Mb of genomic sequences?

If I want to re-sequence a known part of the chromosome (we know the reference genome sequence) of ~1Mb to find the possible mutations (SNP or INDEL) that caused a certain phenotype. What is the best ...
3
votes
1answer
46 views

Effect of closed eyes on balance?

Suppose you want to stand on one leg. Doing it eyes open is not that difficult, but doing it eyes closed seems to be difficult. Why?
3
votes
1answer
22 views

What's up with all the vague protocols? [closed]

I have lost count of how many protocols I've seen, including those supposed to be professionally written (such as manuals that come with kits from well known brands, or methods sections of papers in ...
1
vote
1answer
20 views

Ebola immunosupression and infection

I was wondering... If Ebola attacks immune cells and causes immunosupression, then shouldn't we see death in Ebola patients due to secondary infection?
-2
votes
1answer
53 views

Can a rotten egg actually hatches to a real life?

First of all, I'm no expert in biology. I failed a lot in high school. Please bear with it if it's a stupid question. For the past few days, I got a rotten smell as if an animal died in my kitchen. I ...
3
votes
1answer
79 views

Elevated position effect on recovery times from upper respiratory infections at rest?

In nursing school, they advice for people with upper respiratory infections to be in a slightly elevated position at the head region when sleeping. My intuition of the reason is that the lymphatic ...
1
vote
1answer
29 views

PCR directly on DNA binds to Ampure XP

During the library preparation it's possible to conserved Ampure beads during the different enzymatic reaction (it's the with beads strategy for improving yield, Fisher et al. Genome Biology 2011). ...
1
vote
1answer
38 views

Could someone link me a picture of interstitial fluid? [closed]

Doing a quick Google image search doesn't return much and I'm curious to know what it looks like. Am I correct when I say, since it contains primarily lymph I'd expect it to be clear ? Thanks.
1
vote
0answers
13 views

What is the best way to perform ultra-deep targeted sequencing of few samples?

I performed recent whole genome/exome sequencing in tumor samples and now I would like to take a few of these variants (say maximum 50 amplicons) to: 1) Validate them in the original samples 2) ...
1
vote
1answer
36 views

Is there any way to make pymol find the closest distance between RESIDUES? [closed]

This seems like an obvious utility, but I can only make it find the closest distance between ATOMS, which makes a big mess on the screen. Edit: To be clear, I want to find the closest distance ...
4
votes
2answers
69 views

How does the eye know whether to focus further out or nearer in order to bring a blurry object into focus?

If the eye sees an object, but the object is out of focus, the eye could be focusing too near or too far. How does the eye (or the brain) distinguish one situation from the other? How does it know ...
5
votes
1answer
249 views

Do the right-handed people tend to use the right side teeth of their jaw to chew food more often than the left-handed people?

And vice versa, do the left-handed people tend to use the left side teeth of their jaw to chew food more often than the right-handed people?Or the frequency of food chewing distribute fairly to both ...
5
votes
1answer
47 views

Examples of genes involved in plastic responses

Adaptive plasticity involves sensing the environment and responding adaptively to it. Intuitively, I would think that this process may ask for a more or less complex genetic machinery of regulation of ...
1
vote
0answers
14 views

how to get truseq's gel size selection when preparing libraries for exome enrichment?

i would normally use a 6 minute fragmentation as quoted by agilent but this gives post exome enrichment libraries of around 300bp or slightly over. Truseq want libraries to be 100bp larger. i am ...
3
votes
1answer
44 views

Do we know if RHEB is more sensitive to some amino acids than other amino acids?

RHEB (Ras homolog enriched in brain) senses amino acid levels in order to recruit mTOR. See this review describing the process of how RHEB recruits mTOR. So does RHEB have differential selectivity ...
2
votes
1answer
42 views

Like size and colour constancy, why does the brain not maintain focus constancy?

I have progressive myopia. I grew up with perfectly normal eyes but slowly started getting myopic. The thing is, my brain knows (or should know) what the world looks like -- it knows that far away ...
2
votes
1answer
24 views

Separation of closely-sized isoforms

I have to separate two proteins of 86kDa and 80kDa respectively, however, I just cannot get a decent separation even in 6% polyacrylamide gel. To make matters worse, these two proteins are isoforms ...
2
votes
1answer
67 views

Domains in cell membrane

How is movement of proteins and lipids between different domains of cell membrane prevented? Why is the noncytosolic layer not able to do lateral movements between domains but cytosolic layer is able ...
5
votes
1answer
38 views

Are there any online resources one can use to access raw clinical trial data without having to contact those involved in the study?

I want to do some statistical analysis and I have a preference for real data so I was wondering if there's somewhere I can get such data for free without a heap of bother in phoning other those ...
5
votes
0answers
102 views

Model for fluctuating selection

Is there any mathematical model to predict the behaviour and long-term consequence of counter-acting selection at different time scale? For example, let's consider the bi-allelic gene A, with alleles ...
0
votes
1answer
21 views

Intro Bio question: dihybrid punnet square

Today we had our exam. There was a punnet square for two genes. and when I multiplied , for example, Ry × rY I wrote the answer as ...
0
votes
0answers
20 views

General time reversible model of evolution and Felsenstein model

I would like to get suggestions for the books related to General time reversible model of evolution and Felsenstein model etc. Specifically the mathematical treatment of these topics and concepts ...
4
votes
1answer
70 views

Is eating cooked food an evolved behavior or rather an intelligent one, passed down via culture?

I was just eating a rather rare steak when I started wondering whether eating foods cooked was something I would instinctively want to do if the practice hadn't been taught to me. So, is cooking food ...
-2
votes
1answer
35 views

How does a multicellular, eukaryotic body treat heat-resistant pathogenic bacteria?

How does the said body treat heat-resistant pathogenic bacteria? If fever is the body's response to foreign microbial invasion, then what happens if the bacteria is heat-resistant but the body's own ...
0
votes
1answer
13 views

Exclusive microtubule minus-end labeling

Like the title explains I am looking for a way to exclusively label microtubule minus end in vivo. Looking through the literature I could not find any techniques yet. Do you have an idea?
0
votes
0answers
18 views

Why does some skin hold perfume longer than others

I was wondering my girlfriend and I used the same perfume for a while and we sprayed the same place. And after a day her skin still smells nice while mine smells normal. Will different types of ...
3
votes
1answer
69 views

Is a single x-ray photon capable of doing enough damage to the DNA of a cell to kill that cell? [closed]

Ionizing radiation is damaging to DNA, but is there such a thing as a safe dose below which no DNA damage could occur? I'm not asking about a minimum safe dose overall, just whether or not there's an ...
1
vote
1answer
61 views

Why can't people talk while inhaling?

Why do we have to exhale in order to talk? From looking on Wikipedia, it seems like it has something to do with the glottis, but I'm not clear on the mechanism that makes speech sound so different ...
5
votes
3answers
108 views

What makes DNA sticky-ends sticky?

When restriction enzymes jaggedly cut double stranded DNA it results in so called sticky ends. What is the substance that makes the DNA sticky?
3
votes
2answers
108 views

What is the scientific opinion on plus lens therapy?

Plus lens therapy is the theory that most myopia is environmentally triggered and that conventional corrective minus lenses increase nearsightedness over time. Thus the hypothesis that applying plus ...
1
vote
0answers
53 views

To define the total Resistance of Systemic Blood Circulation

Some systems are in parallel. For instance, the resistance of lungs $R_{\text{lungs}}$. Assume here for simplicity that these parallel systems can be handled linearly. We also consider only Total ...
1
vote
1answer
33 views

Clonidine's adrenonergic nature?

I am little confused here. I used the term adrenoagonist and sympatholytic to describe the compound. However, my teacher says that the correct term here is adrenomimetic -term. My understanding of ...
2
votes
1answer
63 views

Fast standing and the heart's insufficient accommodation of the increased venous return

Assume you are 45 minutes on the supine position. Furthermore: you stand all of a sudden and fast and without sympaticus activity. The venous return (smooth musculature of vessels) accommodates ...
3
votes
2answers
253 views

Why does methylation not occur in viral DNA?

Why does methylation not occur in viral DNA? Can viral DNA undergo the process of methylation? If not then why does this process does not occur in viruses?
4
votes
0answers
45 views

Why do some internal organs regenerate?

I have been reading about the human liver and zebra fish heart muscle having the ability to regenerate. It seems to me that these organs have very little chance to become damaged or worn out. At the ...
1
vote
1answer
69 views

Possibility of Human cloning

Technology has evolved so much as to clone animals. But, what about human cloning? Is it really possible to create humans in lab? Or is it that technology has not advanced to that level..? Why cant ...

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