1
vote
0answers
41 views

Does malaria have a reservoir host?

Can warm blooded vertebrates other than humans act as reservoir hosts for malaria parasites? I'm mainly interested in Plasmodium vivax and possible reservoir hosts in the wider area of Europe/ ...
2
votes
0answers
19 views

Storing Taqman plates with polymerase in fridge before running

I'm going to be running some Taqman assays on cDNA generated from RNA collected from various samples, and will end up running a bunch of plates (right now the setup is 1/2 a plate per sample, ...
2
votes
1answer
25 views

Which is the guideline to choose a molecular target to identify vertebrate hosts from arthropod bloodmeals?

There are some molecular targets to identify vertebrate hosts from arthropod bloodmeals including the Cyt b gene and the COI gene. Which are the standards or characteristics that I have to bear in ...
2
votes
4answers
99 views

Why *morning walk*?

My PT teachers professes working-out/training in early morning hours is the best time to do so, a quick search on benefits of morning walk (or some similar phrase) reveals a lot of information which ...
1
vote
1answer
21 views

specific numbers of neucleotides in okazaki fragments

Why there is specific number of nucleotides in okazaki fragments...? Okazaki fragments are form in lagging strand during the process of DNA replication.
3
votes
1answer
35 views

Mechanism behind negative conductance of ion-channels

I am struggling to understand negative conductance shown on I-V curves on ion channels. Mechanistically negative conductance means that inward (or outward) current increases when voltage across ...
2
votes
1answer
40 views

In insects, does the Alanine repeat occur on the homeodomain sequence of the abdomen or does it occur on a different sequence?

By "Alanine repeat", I am referring to the suppression of the formation of extra insect legs due to Ubx gene suppression through Distal-less repression.
1
vote
1answer
38 views

An example for N50? Why do we need it?

I'm trying to understand N50 on the wikipedia. But I was unable to get a sense of the definition: Given a set of contigs, each with its own length, the N50 length is defined as the length for ...
6
votes
2answers
480 views

Solvent Accessibility, the 20% cut-off method

I'm reading the papers linked below and all three of them mention a 20% cut-off for buried/exposed residues, by calculating a relative solvent accessibility (RSA) value. I understand how the RSA is ...
1
vote
1answer
42 views

How is ATP involved in muscle contraction?

The sliding filament mechanism as explained by my text does not elaborate on how ATP is involved in the cross bridge binding and contraction process. How does muscle contraction utilize ATP? In my ...
2
votes
1answer
38 views

Genetic Imprinting and Cell differentiation

It does not seem possible that these two processes can coexist: 1) Genetic imprinting is the phenomenon where genes are expressed differently depending on the parent of origin: 1a. Methylated ...
13
votes
1answer
267 views

How does Sodium Valproate cause neural plasticity

I have been reading a fascinating paper: Valproate reopens critical-period learning of absolute pitch 18 individuals were given Sodium Valproate (VPA) for a fortnight during which they trained on a ...
2
votes
2answers
41 views

How to compute the regression of individual fitness on individual phenotype

Consider a population structured in groups of two individuals. Individuals' interactions follow an additive prisoner's dilemma: \begin{array}{c |c |c|} & C & D \\ \hline \text{Cooperate (} C ...
9
votes
2answers
8k views

Why do dogs try to cover their poop?

It may seem obvious to us humans to hide our excreta, but this is not quite normal behavior for animals. Interestingly, dogs have the habit to pee all over the place. It's know that they do so to ...
0
votes
1answer
24 views

Without help of Insects, can plants & trees bear fruits & flowers? [closed]

Without help of Insects, can plants & trees bear fruits & flowers?
4
votes
1answer
48 views

Are stem cell lines cryogenically preserved or commercially available like HeLa cells are?

There are HeLa cells, which were taken from sick cancer patient and now is growing worldwide in different laboratories for experiments. HeLa cells can be kept because they are immortal and so they are ...
1
vote
1answer
17 views

Possible to remove accumulated haze on fluorescence interference filters?

I have a few different fluorescent microscopes, the two that use Mercury lamps are older than 7 years. I notice on those that the thin film filters in the filter cubes (green, red, blue) have ...
7
votes
1answer
60 views

Why are cell lines frozen in vapor phase?

For most of the cell lines I've come across, ATCC recommends storing them in the vapor phase of liquid nitrogen. I'm taking that to mean any place above the level of liquid in the nitrogen storage ...
1
vote
1answer
131 views

Interlocus Contest Evolution means suicide?

I was reading Matt Ridley's "Genome" book. I am trying to understand the Interlocus Contest Evolution in his X-Y chapter. I do not understand why the X and Y chromosome would want to kill each other. ...
4
votes
2answers
40 views

Dyes to bind to double stranded DNA?

Are there any commercially available fluorescent dyes that will bind only to double stranded DNA (not RNA, single stranded DNA etc.) for studying in vitro using confocal microscopy?
1
vote
2answers
95 views

Microscope scale bar

In some microscopic images, there is a bar scale at one of the corners or near a specific object. How are they added to the images? Does the slide have some markers, or are they digitally added? If ...
2
votes
1answer
20 views

What are the units of Phylogenetic Independent Contrasts (PICs)

PICs are a popular phylogenetic comparative method and I was wondering what units the actual contrasts are in. I want to say they are unitless but I am not entirely sure.
0
votes
0answers
5 views

Why low variant frequency in exomeseq data (ANNOVAR)?

I've got an annotated ANNOVAR file with 300.000 nucleotide variants of 4 genomes. There's one thing I don't understand when looking at the data: Why are the variant frequencies so low in comparison to ...
1
vote
1answer
61 views

History: Building a “Phylogenetic network” of famous evolutionary biologists [closed]

Seems like many of the famous evolutionary biologists are somehow related to each others. For example (and I might be mistaken), Ronald A. Fisher was mentored by a descendent of Charles Darwin. Joe ...
5
votes
1answer
66 views

Influence of eating at late night on sleep

I have often heard the saying that one shouldn't eat a mountain of food because blood will go to the stomach and it'll be difficult to sleep well because the stomach can't rest at night. I see that ...
3
votes
1answer
30 views

How could I identify whether given RNA-seq data is paired end or single end

I need to have a RNA-Seq dataset and therefore, I've visited the following site NCBI-geo C. Elegans In the Supplemantary file part, I clicked the SRP/SRP051/SRP051702 ftp and downloaded sra file. ...
1
vote
1answer
190 views

What's the difference between stabilizing selection and balancing selection?

I came across these terms in Darwin's "Origin of Species" and I wasn't sure what the difference is.
0
votes
1answer
18 views

What does this mutation notation mean: MAP2K41161fs*12

I received a report that had a mutation notated as: "MAP2K41161fs*12". I'm used to seeing this sort of thing: BRAF(V600E) or BRAF(T1799TA). Can someone tell me how to interpret "MAP2K41161fs*12" ?? Is ...
3
votes
5answers
459 views

Does any animal species have two sexes and more genders?

Does it exist any animal species that have two sexes and a kind of different internal diversification in two genders or more? I mean no something like different-task-based diversity but something ...
3
votes
0answers
36 views

Colored water uptake by plants [Rainbow Roses]

We all know cut flowers in a vase with water will drink the water. The water is transported by the xylem and by passing through the cellular walls reaches every plant cell and hydrates it or it ...
8
votes
2answers
26k views

What is the function of the RNA primer in DNA replication?

During DNA replication, RNA primase puts an RNA primer in the lagging strand. What is the function of this RNA primer? Why can't the enzymes put DNA fragments directly?
3
votes
1answer
141 views

Lentivector biosafety

Lentivectors are widely used in molecular biology, most commonly to transduce in a stable way a desired gene. These vector system takes advantage from the ability of viruses to introduce their own ...
2
votes
2answers
32 views

How to conceptualize the action potential?

In my AP Biology class, we were taught that action potentials are not electrical impulses in the same way current travels through a wire. Rather, we were taught that action potentials are changing ...
1
vote
2answers
59 views

Trying to understand the big picture behind DNA sequencing, alignment and searching

I'm about to start a bioinformatics research project but I haven't any biological background. I know my project is in regards to a performance analysis of DNA sequencing and searching "weapons" like ...
37
votes
4answers
2k views

What exactly are computers used for in DNA sequencing?

I've thoroughly read the Wikipedia article on DNA sequencing and can't get one thing. There's some hardcore chemistry involved in the process that somehow splits the DNA and then isolates its parts. ...
25
votes
4answers
6k views

What does the human body use oxygen for besides the final electron acceptor in the electron transport chain?

My biology teachers never explained why animals need to breathe oxygen, just that we organisms die if we don't get oxygen for too long. Maybe one of them happened to mention that its used to make ATP. ...
1
vote
1answer
23 views

Are there fish with a double circulatory system?

Our courseware materials say there are such fish, but the internet suggests fish only have a single circulatory system. Thanks!
0
votes
0answers
13 views

Linkage Repulsion

"Segregation distorters such as meiotic drivers, involve two loci—a killer (drive) locus and a sensitivity (target) locus—being in strong linkage repulsion to ensure that the drive and ...
1
vote
0answers
38 views

Drosophila biarmipes gene annotation, transposable element?

When doing an annotation of a contig31 of Drosophila biarmipes genome, the gene predictions(in orange, red, green, tan, blue, brown, and gray) picked up numerous possible genes in the area circled in ...
1
vote
1answer
77 views

How do you convert mtDNA sequences in FASTA to FSTAT format?

I've got control region sequence data from a population of shark and I'm looking to convert this from FASTA to FSTAT in order to calculated the effective population size of females. The software I ...
0
votes
1answer
52 views

Mutations/deletions with CRISPR

I need to stop some protein from being active and searching for some universal way to do so. In mammalians. With CRISPR it is possible to knock-out the entire gene. But it's a little complicate ...
2
votes
1answer
58 views

How is protein cavity centre related to binding?

I am confused and I have the following questions: 1. What are (in the context of article below) protein cavity centres? 2. How are they related to binding? (Automated identification of protein-ligand ...
0
votes
1answer
31 views

Genetic notations

Genetic testing revealed these two mutations (hypothetically): IVS11+6G>A and IVS11-4G>A Could you please explain every part of this notation, especially "+" and "-" signs.
3
votes
2answers
53 views

What physical force attracts the anti-codon on tRNA to the codon on mRNA during translation?

What physical force attracts the anti-codon on tRNA to the codon on mRNA during translation? I know that these two bond together, but what actually makes the tRNA move through the cytoplasm to the ...
0
votes
3answers
82 views

How does frame-shift mutation not absolutely ruin the organism?

I'm a bit curious as to how frame-shift mutation works. If you shift one amino acid towards another. How does this not affect the entire chromosome? Wouldn't this mean that the organism would be ...
7
votes
1answer
145 views

Why do only humans commit suicide?

Why is it that only humans commit suicide? Many animals do 'brave' things to protect their children or family, and some male spiders sacrifice themselves so that they can impregnate the females. ...
3
votes
2answers
37 views

Macrophages and what they consume

Do macrophages in the dermis consume any foreign invader to the body, or simply pathogens and cellular debris? For example, would macrophages consume vitamins or medications injected into the dermal ...
7
votes
0answers
67 views

Biopython - Big Discrepancy Calculating RNA melting Temperature over Literature

I experience big discrepancies when calculating melting temperature of RNA 7-mers with Biopython over values generated by a popular algorithm. I tried the nearest neighbour algorithm with RNA and ...
8
votes
2answers
819 views

Do males of all sexual species have Y chromosomes?

I know for instance some cells are sexual, so, this got me wondering, do the males of all species that have distinct sexes have Y chromosomes?
2
votes
1answer
57 views

Are there any examples of how to apply evolutionary models?

Apologies if this question it too open-ended; evolutionary biology is not my primary field. I have been reading a lot about the use of statistical mechanics in analyzing evolutionary dynamics. As an ...

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